Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure
The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytet...
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| Veröffentlicht in: | International journal of environmental research and public health 2021-03, Vol.18 (6), p.3218 |
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| creator | Almeida, Ana Rita Tacão, Marta Soares, Joana Domingues, Inês Henriques, Isabel |
| description | The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of
genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with
and
. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the
gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS. |
| doi_str_mv | 10.3390/ijerph18063218 |
| format | Article |
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genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with
and
. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the
gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18063218</identifier><identifier>PMID: 33804606</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiotensin Receptor Antagonists ; Angiotensin-Converting Enzyme Inhibitors ; Animals ; Anti-Bacterial Agents - toxicity ; Antibiotic resistance ; Antibiotics ; Aquaculture ; Bacteria ; Bacteria - genetics ; Contamination ; Danio rerio ; Drug resistance ; Embryos ; Experiments ; Exposure ; Food ; Genes ; Genes, Bacterial ; Membranes ; Multidrug resistance ; Multidrug resistant organisms ; Oxytetracycline ; Pathogens ; Selective media ; Statistical analysis ; Statistical methods ; Surface water ; TetA gene ; Water ; Zebrafish</subject><ispartof>International journal of environmental research and public health, 2021-03, Vol.18 (6), p.3218</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-a91b90266683c11a88c54645d3f06eb520d93c976522a5f66fff77eaee796d903</citedby><cites>FETCH-LOGICAL-c418t-a91b90266683c11a88c54645d3f06eb520d93c976522a5f66fff77eaee796d903</cites><orcidid>0000-0003-1123-9167 ; 0000-0001-7965-4928 ; 0000-0001-7717-4939</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003806/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003806/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33804606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Almeida, Ana Rita</creatorcontrib><creatorcontrib>Tacão, Marta</creatorcontrib><creatorcontrib>Soares, Joana</creatorcontrib><creatorcontrib>Domingues, Inês</creatorcontrib><creatorcontrib>Henriques, Isabel</creatorcontrib><title>Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>The emergence of antibiotic-resistant pathogens due to worldwide antibiotic use is raising concern in several settings, including aquaculture. In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of
genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with
and
. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the
gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. 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In this work, the selection of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) was evaluated after exposure of zebrafish to oxytetracycline (OTC) for two months, followed by a recovery period. The selection of ARB in water and fish was determined using selective media. The abundance of
genes was estimated through qPCR. Higher prevalence of ARB was measured in all samples exposed to the antibiotic when compared to control samples, although statistical significance was only achieved five days after exposure. Isolates recovered from samples exposed to the antibiotic were affiliated with
and
. Various antibiotic susceptibility profiles were detected and 37% of the isolates displayed multidrug resistance (MDR). The selection of the
gene was confirmed by qPCR at the highest OTC concentration tested. Two MDR isolates, tested using zebrafish embryos, caused significant mortality, indicating a potential impact on fish health and survival. Overall, our work highlights the potential impact of antibiotic contamination in the selection of potential pathogenic ARB and ARGS.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33804606</pmid><doi>10.3390/ijerph18063218</doi><orcidid>https://orcid.org/0000-0003-1123-9167</orcidid><orcidid>https://orcid.org/0000-0001-7965-4928</orcidid><orcidid>https://orcid.org/0000-0001-7717-4939</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Angiotensin Receptor Antagonists Angiotensin-Converting Enzyme Inhibitors Animals Anti-Bacterial Agents - toxicity Antibiotic resistance Antibiotics Aquaculture Bacteria Bacteria - genetics Contamination Danio rerio Drug resistance Embryos Experiments Exposure Food Genes Genes, Bacterial Membranes Multidrug resistance Multidrug resistant organisms Oxytetracycline Pathogens Selective media Statistical analysis Statistical methods Surface water TetA gene Water Zebrafish |
| title | Tetracycline-Resistant Bacteria Selected from Water and Zebrafish after Antibiotic Exposure |
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