Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones
DNA methylation is known as a biomarker for age with applications in forensics. Here we describe the VISAGE (VISible Attributes through GEnomics) Consortium's enhanced tool for epigenetic age estimation in somatic tissues. The tool is based on eight DNA methylation markers (44 CpGs), bisulfite...
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| Veröffentlicht in: | Aging (Albany, NY.) NY.), 2021-03, Vol.13 (5), p.6459-6484 |
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| creator | Woźniak, Anna Heidegger, Antonia Piniewska-Róg, Danuta Pośpiech, Ewelina Xavier, Catarina Pisarek, Aleksandra Kartasińska, Ewa Boroń, Michał Freire-Aradas, Ana Wojtas, Marta de la Puente, Maria Niederstätter, Harald Płoski, Rafał Spólnicka, Magdalena Kayser, Manfred Phillips, Christopher Parson, Walther Branicki, Wojciech |
| description | DNA methylation is known as a biomarker for age with applications in forensics. Here we describe the VISAGE (VISible Attributes through GEnomics) Consortium's enhanced tool for epigenetic age estimation in somatic tissues. The tool is based on eight DNA methylation markers (44 CpGs), bisulfite multiplex PCR followed by sequencing on the MiSeq FGx platform, and three statistical prediction models for blood, buccal cells and bones. The model for blood is based on six CpGs from
,
,
,
,
and
, and predicts age with a mean absolute error (MAE) of 3.2 years, while the model for buccal cells includes five CpGs from
,
,
,
and
and predicts age with MAE of 3.7 years, and the model for bones has six CpGs from
and
and predicts age with MAE of 3.4 years. The VISAGE enhanced tool for age estimation in somatic tissues enables reliable collection of DNA methylation data from small amounts of DNA using a sensitive multiplex MPS assay that provides accurate estimation of age in blood, buccal swabs, and bones using the statistical model tailored to each tissue. |
| doi_str_mv | 10.18632/aging.202783 |
| format | Article |
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,
,
,
,
and
, and predicts age with a mean absolute error (MAE) of 3.2 years, while the model for buccal cells includes five CpGs from
,
,
,
and
and predicts age with MAE of 3.7 years, and the model for bones has six CpGs from
and
and predicts age with MAE of 3.4 years. The VISAGE enhanced tool for age estimation in somatic tissues enables reliable collection of DNA methylation data from small amounts of DNA using a sensitive multiplex MPS assay that provides accurate estimation of age in blood, buccal swabs, and bones using the statistical model tailored to each tissue.</description><identifier>ISSN: 1945-4589</identifier><identifier>EISSN: 1945-4589</identifier><identifier>DOI: 10.18632/aging.202783</identifier><identifier>PMID: 33707346</identifier><language>eng</language><publisher>United States: Impact Journals</publisher><subject>Research Paper</subject><ispartof>Aging (Albany, NY.), 2021-03, Vol.13 (5), p.6459-6484</ispartof><rights>Copyright: © 2021 Woźniak et al.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-961e93e6f4bc1a75e297ddcff2d2d9c5a7f9103e83a4c716e66349206ed7e5d93</citedby><cites>FETCH-LOGICAL-c453t-961e93e6f4bc1a75e297ddcff2d2d9c5a7f9103e83a4c716e66349206ed7e5d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993733/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993733/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33707346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woźniak, Anna</creatorcontrib><creatorcontrib>Heidegger, Antonia</creatorcontrib><creatorcontrib>Piniewska-Róg, Danuta</creatorcontrib><creatorcontrib>Pośpiech, Ewelina</creatorcontrib><creatorcontrib>Xavier, Catarina</creatorcontrib><creatorcontrib>Pisarek, Aleksandra</creatorcontrib><creatorcontrib>Kartasińska, Ewa</creatorcontrib><creatorcontrib>Boroń, Michał</creatorcontrib><creatorcontrib>Freire-Aradas, Ana</creatorcontrib><creatorcontrib>Wojtas, Marta</creatorcontrib><creatorcontrib>de la Puente, Maria</creatorcontrib><creatorcontrib>Niederstätter, Harald</creatorcontrib><creatorcontrib>Płoski, Rafał</creatorcontrib><creatorcontrib>Spólnicka, Magdalena</creatorcontrib><creatorcontrib>Kayser, Manfred</creatorcontrib><creatorcontrib>Phillips, Christopher</creatorcontrib><creatorcontrib>Parson, Walther</creatorcontrib><creatorcontrib>Branicki, Wojciech</creatorcontrib><creatorcontrib>VISAGE Consortium</creatorcontrib><title>Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones</title><title>Aging (Albany, NY.)</title><addtitle>Aging (Albany NY)</addtitle><description>DNA methylation is known as a biomarker for age with applications in forensics. Here we describe the VISAGE (VISible Attributes through GEnomics) Consortium's enhanced tool for epigenetic age estimation in somatic tissues. The tool is based on eight DNA methylation markers (44 CpGs), bisulfite multiplex PCR followed by sequencing on the MiSeq FGx platform, and three statistical prediction models for blood, buccal cells and bones. The model for blood is based on six CpGs from
,
,
,
,
and
, and predicts age with a mean absolute error (MAE) of 3.2 years, while the model for buccal cells includes five CpGs from
,
,
,
and
and predicts age with MAE of 3.7 years, and the model for bones has six CpGs from
and
and predicts age with MAE of 3.4 years. The VISAGE enhanced tool for age estimation in somatic tissues enables reliable collection of DNA methylation data from small amounts of DNA using a sensitive multiplex MPS assay that provides accurate estimation of age in blood, buccal swabs, and bones using the statistical model tailored to each tissue.</description><subject>Research Paper</subject><issn>1945-4589</issn><issn>1945-4589</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpVkc1P3DAQxa2qqNClx14rH3sg4G_Hl0po-SgSEgdarpZjT7KpEnsbZ5E48L_jZbeInsYa_-bNPD2EvlJySmvF2Znr-tidMsJ0zT-gI2qErISszcd370P0Oec_hCgphfqEDjnXRHOhjtDzBTzCkNYjxBmnFs8rwA839-fXlxjiykUPAc8pDdjFgPPs5j7PvXcDHlOAIeM2TRjWfQcRSh-7DjAUYixgiriPuBlSCie42fjtlIehDG21mhQhH6OD1g0ZvuzrAv2-uvy1_Fnd3l3fLM9vKy8knyujKBgOqhWNp05LYEaH4NuWBRaMl063hhIONXfCa6pAKS4MIwqCBhkMX6AfO931phkh-GJ2coNdT-XQ6ckm19v_f2K_sl16tNoYrjkvAt_3AlP6uykO7djnrRkXIW2yZZJQpmotVEGrHeqnlPME7dsaSuxrZPY1MruLrPDf3t_2Rv_LiL8AmsaVcQ</recordid><startdate>20210311</startdate><enddate>20210311</enddate><creator>Woźniak, Anna</creator><creator>Heidegger, Antonia</creator><creator>Piniewska-Róg, Danuta</creator><creator>Pośpiech, Ewelina</creator><creator>Xavier, Catarina</creator><creator>Pisarek, Aleksandra</creator><creator>Kartasińska, Ewa</creator><creator>Boroń, Michał</creator><creator>Freire-Aradas, Ana</creator><creator>Wojtas, Marta</creator><creator>de la Puente, Maria</creator><creator>Niederstätter, Harald</creator><creator>Płoski, Rafał</creator><creator>Spólnicka, Magdalena</creator><creator>Kayser, Manfred</creator><creator>Phillips, Christopher</creator><creator>Parson, Walther</creator><creator>Branicki, Wojciech</creator><general>Impact Journals</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210311</creationdate><title>Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones</title><author>Woźniak, Anna ; 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Here we describe the VISAGE (VISible Attributes through GEnomics) Consortium's enhanced tool for epigenetic age estimation in somatic tissues. The tool is based on eight DNA methylation markers (44 CpGs), bisulfite multiplex PCR followed by sequencing on the MiSeq FGx platform, and three statistical prediction models for blood, buccal cells and bones. The model for blood is based on six CpGs from
,
,
,
,
and
, and predicts age with a mean absolute error (MAE) of 3.2 years, while the model for buccal cells includes five CpGs from
,
,
,
and
and predicts age with MAE of 3.7 years, and the model for bones has six CpGs from
and
and predicts age with MAE of 3.4 years. The VISAGE enhanced tool for age estimation in somatic tissues enables reliable collection of DNA methylation data from small amounts of DNA using a sensitive multiplex MPS assay that provides accurate estimation of age in blood, buccal swabs, and bones using the statistical model tailored to each tissue.</abstract><cop>United States</cop><pub>Impact Journals</pub><pmid>33707346</pmid><doi>10.18632/aging.202783</doi><tpages>26</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Research Paper |
| title | Development of the VISAGE enhanced tool and statistical models for epigenetic age estimation in blood, buccal cells and bones |
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