COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative...
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| Veröffentlicht in: | International journal of environmental research and public health 2021-03, Vol.18 (5), p.2650 |
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| creator | Vetrugno, Giuseppe La Milia, Daniele Ignazio D'Ambrosio, Floriana Di Pumpo, Marcello Pastorino, Roberta Boccia, Stefania Ricci, Rosalba De-Giorgio, Fabio Cicconi, Michela Foti, Federica Pascucci, Domenico Castrini, Francesco Carini, Elettra Cambieri, Andrea D'Alfonso, Maria Elena Capalbo, Gennaro Fantoni, Massimo Moscato, Umberto Staiti, Domenico De Simone, Francesco Maria Berloco, Filippo Damiani, Gianfranco Zega, Maurizio Cattani, Paola Posteraro, Brunella Sanguinetti, Maurizio Laurenti, Patrizia |
| description | Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (
< 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test's predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress. |
| doi_str_mv | 10.3390/ijerph18052650 |
| format | Article |
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< 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test's predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18052650</identifier><identifier>PMID: 33800721</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antibodies ; Antibodies, Viral ; Blood ; Blood tests ; Confidence intervals ; Coronaviruses ; COVID-19 ; Disease transmission ; Epidemics ; Health care ; Health Personnel ; Hospitals ; Humans ; Immunoassay ; Immunoglobulin G ; Infections ; Laboratories ; Medical personnel ; Personal protective equipment ; Polymerase chain reaction ; Population ; Rome ; SARS-CoV-2 ; Sensitivity ; Sensitivity analysis ; Seroepidemiologic Studies ; Serologic Tests ; Serological tests ; Serology ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Telecommuting</subject><ispartof>International journal of environmental research and public health, 2021-03, Vol.18 (5), p.2650</ispartof><rights>2021. 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Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (
< 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test's predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress.</description><subject>Antibodies</subject><subject>Antibodies, Viral</subject><subject>Blood</subject><subject>Blood tests</subject><subject>Confidence intervals</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Disease transmission</subject><subject>Epidemics</subject><subject>Health care</subject><subject>Health Personnel</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoglobulin G</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Medical personnel</subject><subject>Personal protective equipment</subject><subject>Polymerase chain reaction</subject><subject>Population</subject><subject>Rome</subject><subject>SARS-CoV-2</subject><subject>Sensitivity</subject><subject>Sensitivity analysis</subject><subject>Seroepidemiologic 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are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (
< 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test's predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33800721</pmid><doi>10.3390/ijerph18052650</doi><orcidid>https://orcid.org/0000-0002-5804-2284</orcidid><orcidid>https://orcid.org/0000-0002-9422-3285</orcidid><orcidid>https://orcid.org/0000-0003-3028-6188</orcidid><orcidid>https://orcid.org/0000-0002-0347-088X</orcidid><orcidid>https://orcid.org/0000-0001-5752-0064</orcidid><orcidid>https://orcid.org/0000-0001-6913-8460</orcidid><orcidid>https://orcid.org/0000-0001-7433-0924</orcidid><orcidid>https://orcid.org/0000-0002-9780-7059</orcidid><orcidid>https://orcid.org/0000-0002-1864-749X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1660-4601 |
| ispartof | International journal of environmental research and public health, 2021-03, Vol.18 (5), p.2650 |
| issn | 1660-4601 1661-7827 1660-4601 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7967358 |
| source | PubMed (Medline); MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Antibodies Antibodies, Viral Blood Blood tests Confidence intervals Coronaviruses COVID-19 Disease transmission Epidemics Health care Health Personnel Hospitals Humans Immunoassay Immunoglobulin G Infections Laboratories Medical personnel Personal protective equipment Polymerase chain reaction Population Rome SARS-CoV-2 Sensitivity Sensitivity analysis Seroepidemiologic Studies Serologic Tests Serological tests Serology Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Telecommuting |
| title | COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T10%3A13%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=COVID-19%20Seroprevalence%20among%20Healthcare%20Workers%20of%20a%20Large%20COVID-19%20Hospital%20in%20Rome%20Reveals%20Strengths%20and%20Limits%20of%20Two%20Different%20Serological%20Tests&rft.jtitle=International%20journal%20of%20environmental%20research%20and%20public%20health&rft.au=Vetrugno,%20Giuseppe&rft.date=2021-03-06&rft.volume=18&rft.issue=5&rft.spage=2650&rft.pages=2650-&rft.issn=1660-4601&rft.eissn=1660-4601&rft_id=info:doi/10.3390/ijerph18052650&rft_dat=%3Cproquest_pubme%3E2500003643%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2500003643&rft_id=info:pmid/33800721&rfr_iscdi=true |