High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The nu...
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| Veröffentlicht in: | Scientific reports 2021-03, Vol.11 (1), p.5874-5874, Article 5874 |
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| creator | Kim, Jieun Park, Kye-Yeung Park, Hoon-Ki Hwang, Hwan-Sik Seo, Mi-Ran Kim, Bongyoung Cho, Youna Rho, Mina Pai, Hyunjoo |
| description | To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low ( |
| doi_str_mv | 10.1038/s41598-021-84974-4 |
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| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7955109</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2501262189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3444-16e8bf23ff26cbf24a4d551bdd97955090a84a0bef5c589b8c38b2425e85f3d83</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhSMEolXpC7CyxIZFU_ybcTZIaAS0ohWbIsHKunFuMq4ce2onSPMWPDKemYq_Bd74SD7ns69PVb1k9JJRod9kyVSra8pZrWW7krV8Up1yKlXNBedP_9An1XnO97QsxVvJ2ufViRArxhvVnFY_rty4IQNa8MRCSg5GJHEgnYf13df69uKgbr_V_IJA6MnWQ55cX0_YO5ixJw-LC9HHgCRhdnmGYJGMGDATmGIYyQbBz5sd-RQTQiBbjFuPxAUCZMK5XBfi5GyBg98VwIvq2QA-4_njflZ9-fD-bn1V33z-eL1-d1NbIaWsWYO6G7gYBt7YIiTIXinW9X27apWiLQUtgXY4KKt022krdMclV6jVIHotzqq3R-526cowFsOcwJttchOknYngzN8nwW3MGL-bPZ7RtgBePwJSfFgwz2Zy2aL3EDAu2XBFyx9zpvfWV_9Y7-OSysAHF210o7QoLn502RRzTjj8egyjZt-5OXZuSufm0LmRJSSOoVzMYcT0G_2f1E-bQ66h</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2500686583</pqid></control><display><type>article</type><title>High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis</title><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kim, Jieun ; Park, Kye-Yeung ; Park, Hoon-Ki ; Hwang, Hwan-Sik ; Seo, Mi-Ran ; Kim, Bongyoung ; Cho, Youna ; Rho, Mina ; Pai, Hyunjoo</creator><creatorcontrib>Kim, Jieun ; Park, Kye-Yeung ; Park, Hoon-Ki ; Hwang, Hwan-Sik ; Seo, Mi-Ran ; Kim, Bongyoung ; Cho, Youna ; Rho, Mina ; Pai, Hyunjoo</creatorcontrib><description>To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low (< 60 GPM) ARG groups. Individuals in the high ARG group tended to visit hospitals more often (
P
= 0.065), particularly for upper respiratory tract infections (
P
= 0.066), and carried more
bla
CTX-M
(
P
= 0.008). The targeted metagenome approach for
bla
and plasmid-mediated quinolone resistance (PMQR) genes revealed a high fecal carriage rate; 23% or 13.1% of the subjects carried
bla
CTX-M
or
bla
CMY-2
, respectively. Regarding PMQR genes, 59% of the subjects carried PMQR, and 83% of them harboured 2‒4 PMQR genes (
qnrB
44.3%,
qnrS
47.5% etc.). The presence of
bla
CTX-M
correlated with ARG abundance in the gut resistome, whereas PMQR genes were irrelevant to other ARGs (
P
= 0.176). Fecal carriage of
bla
CTX-M
and PMQR genes was broad and multiplexed among healthy individuals.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-84974-4</identifier><identifier>PMID: 33712656</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/22 ; 631/326/41/2142 ; Abundance ; Antibiotic resistance ; Feces ; Humanities and Social Sciences ; Intestinal microflora ; Metagenomics ; Microbiomes ; multidisciplinary ; Respiratory tract ; Respiratory tract diseases ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2021-03, Vol.11 (1), p.5874-5874, Article 5874</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3444-16e8bf23ff26cbf24a4d551bdd97955090a84a0bef5c589b8c38b2425e85f3d83</citedby><cites>FETCH-LOGICAL-c3444-16e8bf23ff26cbf24a4d551bdd97955090a84a0bef5c589b8c38b2425e85f3d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955109/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955109/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids></links><search><creatorcontrib>Kim, Jieun</creatorcontrib><creatorcontrib>Park, Kye-Yeung</creatorcontrib><creatorcontrib>Park, Hoon-Ki</creatorcontrib><creatorcontrib>Hwang, Hwan-Sik</creatorcontrib><creatorcontrib>Seo, Mi-Ran</creatorcontrib><creatorcontrib>Kim, Bongyoung</creatorcontrib><creatorcontrib>Cho, Youna</creatorcontrib><creatorcontrib>Rho, Mina</creatorcontrib><creatorcontrib>Pai, Hyunjoo</creatorcontrib><title>High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low (< 60 GPM) ARG groups. Individuals in the high ARG group tended to visit hospitals more often (
P
= 0.065), particularly for upper respiratory tract infections (
P
= 0.066), and carried more
bla
CTX-M
(
P
= 0.008). The targeted metagenome approach for
bla
and plasmid-mediated quinolone resistance (PMQR) genes revealed a high fecal carriage rate; 23% or 13.1% of the subjects carried
bla
CTX-M
or
bla
CMY-2
, respectively. Regarding PMQR genes, 59% of the subjects carried PMQR, and 83% of them harboured 2‒4 PMQR genes (
qnrB
44.3%,
qnrS
47.5% etc.). The presence of
bla
CTX-M
correlated with ARG abundance in the gut resistome, whereas PMQR genes were irrelevant to other ARGs (
P
= 0.176). Fecal carriage of
bla
CTX-M
and PMQR genes was broad and multiplexed among healthy individuals.</description><subject>631/326/22</subject><subject>631/326/41/2142</subject><subject>Abundance</subject><subject>Antibiotic resistance</subject><subject>Feces</subject><subject>Humanities and Social Sciences</subject><subject>Intestinal microflora</subject><subject>Metagenomics</subject><subject>Microbiomes</subject><subject>multidisciplinary</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAUhSMEolXpC7CyxIZFU_ybcTZIaAS0ohWbIsHKunFuMq4ce2onSPMWPDKemYq_Bd74SD7ns69PVb1k9JJRod9kyVSra8pZrWW7krV8Up1yKlXNBedP_9An1XnO97QsxVvJ2ufViRArxhvVnFY_rty4IQNa8MRCSg5GJHEgnYf13df69uKgbr_V_IJA6MnWQ55cX0_YO5ixJw-LC9HHgCRhdnmGYJGMGDATmGIYyQbBz5sd-RQTQiBbjFuPxAUCZMK5XBfi5GyBg98VwIvq2QA-4_njflZ9-fD-bn1V33z-eL1-d1NbIaWsWYO6G7gYBt7YIiTIXinW9X27apWiLQUtgXY4KKt022krdMclV6jVIHotzqq3R-526cowFsOcwJttchOknYngzN8nwW3MGL-bPZ7RtgBePwJSfFgwz2Zy2aL3EDAu2XBFyx9zpvfWV_9Y7-OSysAHF210o7QoLn502RRzTjj8egyjZt-5OXZuSufm0LmRJSSOoVzMYcT0G_2f1E-bQ66h</recordid><startdate>20210312</startdate><enddate>20210312</enddate><creator>Kim, Jieun</creator><creator>Park, Kye-Yeung</creator><creator>Park, Hoon-Ki</creator><creator>Hwang, Hwan-Sik</creator><creator>Seo, Mi-Ran</creator><creator>Kim, Bongyoung</creator><creator>Cho, Youna</creator><creator>Rho, Mina</creator><creator>Pai, Hyunjoo</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210312</creationdate><title>High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis</title><author>Kim, Jieun ; Park, Kye-Yeung ; Park, Hoon-Ki ; Hwang, Hwan-Sik ; Seo, Mi-Ran ; Kim, Bongyoung ; Cho, Youna ; Rho, Mina ; Pai, Hyunjoo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3444-16e8bf23ff26cbf24a4d551bdd97955090a84a0bef5c589b8c38b2425e85f3d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/326/22</topic><topic>631/326/41/2142</topic><topic>Abundance</topic><topic>Antibiotic resistance</topic><topic>Feces</topic><topic>Humanities and Social Sciences</topic><topic>Intestinal microflora</topic><topic>Metagenomics</topic><topic>Microbiomes</topic><topic>multidisciplinary</topic><topic>Respiratory tract</topic><topic>Respiratory tract diseases</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jieun</creatorcontrib><creatorcontrib>Park, Kye-Yeung</creatorcontrib><creatorcontrib>Park, Hoon-Ki</creatorcontrib><creatorcontrib>Hwang, Hwan-Sik</creatorcontrib><creatorcontrib>Seo, Mi-Ran</creatorcontrib><creatorcontrib>Kim, Bongyoung</creatorcontrib><creatorcontrib>Cho, Youna</creatorcontrib><creatorcontrib>Rho, Mina</creatorcontrib><creatorcontrib>Pai, Hyunjoo</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jieun</au><au>Park, Kye-Yeung</au><au>Park, Hoon-Ki</au><au>Hwang, Hwan-Sik</au><au>Seo, Mi-Ran</au><au>Kim, Bongyoung</au><au>Cho, Youna</au><au>Rho, Mina</au><au>Pai, Hyunjoo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2021-03-12</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><spage>5874</spage><epage>5874</epage><pages>5874-5874</pages><artnum>5874</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low (< 60 GPM) ARG groups. Individuals in the high ARG group tended to visit hospitals more often (
P
= 0.065), particularly for upper respiratory tract infections (
P
= 0.066), and carried more
bla
CTX-M
(
P
= 0.008). The targeted metagenome approach for
bla
and plasmid-mediated quinolone resistance (PMQR) genes revealed a high fecal carriage rate; 23% or 13.1% of the subjects carried
bla
CTX-M
or
bla
CMY-2
, respectively. Regarding PMQR genes, 59% of the subjects carried PMQR, and 83% of them harboured 2‒4 PMQR genes (
qnrB
44.3%,
qnrS
47.5% etc.). The presence of
bla
CTX-M
correlated with ARG abundance in the gut resistome, whereas PMQR genes were irrelevant to other ARGs (
P
= 0.176). Fecal carriage of
bla
CTX-M
and PMQR genes was broad and multiplexed among healthy individuals.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33712656</pmid><doi>10.1038/s41598-021-84974-4</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/326/22 631/326/41/2142 Abundance Antibiotic resistance Feces Humanities and Social Sciences Intestinal microflora Metagenomics Microbiomes multidisciplinary Respiratory tract Respiratory tract diseases Science Science (multidisciplinary) |
| title | High fecal carriage of blaCTX-M, blaCMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis |
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