A clinical investigation into the microbiological status of 'locally infected' leg ulcers
Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG. A clinical investigation into the microbiological status of ‘locally infected’ leg ulcers The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date hav...
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| Veröffentlicht in: | International wound journal 2009-12, Vol.6 (6), p.453-462 |
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| creator | Cooper, Rose A Ameen, Hanar Price, Patricia McCulloch, Dorothy A Harding, Keith G |
| description | Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG. A clinical investigation into the microbiological status of ‘locally infected’ leg ulcers
The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date have dealt with either those organisms that cause infections or those that establish long‐term colonisations. The objective of this open, prospective pilot study was to quantitatively and qualitatively assess the microbiological status of ‘locally infected’ venous leg ulcers. Three different sampling techniques were used to recover organisms from 20 venous leg ulcers that had failed to progress towards healing and comparisons made to explore the distribution of colonised flora within the wound. In total, 116 isolates were recovered (mean of 5·8 per ulcer) with highest recovery rates seen with swabs. Greatest agreement of colonisation and counts was found between swabs and absorbent polyvinyl acetate (PVA) foam disc, and also between PVA discs and biopsies. Lowest agreement was between the distribution of anaerobes and coryneforms in swabs and biopsies, suggesting uneven vertical distribution within ulcers. No justification for using routine biopsies in locally infected venous leg ulcers was found. |
| doi_str_mv | 10.1111/j.1742-481X.2009.00640.x |
| format | Article |
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The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date have dealt with either those organisms that cause infections or those that establish long‐term colonisations. The objective of this open, prospective pilot study was to quantitatively and qualitatively assess the microbiological status of ‘locally infected’ venous leg ulcers. Three different sampling techniques were used to recover organisms from 20 venous leg ulcers that had failed to progress towards healing and comparisons made to explore the distribution of colonised flora within the wound. In total, 116 isolates were recovered (mean of 5·8 per ulcer) with highest recovery rates seen with swabs. Greatest agreement of colonisation and counts was found between swabs and absorbent polyvinyl acetate (PVA) foam disc, and also between PVA discs and biopsies. Lowest agreement was between the distribution of anaerobes and coryneforms in swabs and biopsies, suggesting uneven vertical distribution within ulcers. No justification for using routine biopsies in locally infected venous leg ulcers was found.</description><identifier>ISSN: 1742-4801</identifier><identifier>EISSN: 1742-481X</identifier><identifier>DOI: 10.1111/j.1742-481X.2009.00640.x</identifier><identifier>PMID: 20051097</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aged ; Aged, 80 and over ; Bacteria - isolation & purification ; Bacterial Infections - microbiology ; Bacterial Infections - pathology ; Bioburden ; Biopsy ; Diagnosis, Differential ; Female ; Humans ; Leg Ulcer - microbiology ; Leg Ulcer - pathology ; Localised wound infection ; Male ; Middle Aged ; Original ; Venous leg ulcers ; Wound Infection - microbiology ; Wound Infection - pathology ; Wound swabs</subject><ispartof>International wound journal, 2009-12, Vol.6 (6), p.453-462</ispartof><rights>2009 The Authors. Journal Compilation © 2009 Blackwell Publishing Ltd and Medicalhelplines.com Inc</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5100-d3278b417ecc2255b2b481562ff02eb7ef9c178c968402c7ebbbb733b7583e83</citedby><cites>FETCH-LOGICAL-c5100-d3278b417ecc2255b2b481562ff02eb7ef9c178c968402c7ebbbb733b7583e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951578/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951578/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1742-481X.2009.00640.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20051097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cooper, Rose A</creatorcontrib><creatorcontrib>Ameen, Hanar</creatorcontrib><creatorcontrib>Price, Patricia</creatorcontrib><creatorcontrib>McCulloch, Dorothy A</creatorcontrib><creatorcontrib>Harding, Keith G</creatorcontrib><title>A clinical investigation into the microbiological status of 'locally infected' leg ulcers</title><title>International wound journal</title><addtitle>Int Wound J</addtitle><description>Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG. A clinical investigation into the microbiological status of ‘locally infected’ leg ulcers
The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date have dealt with either those organisms that cause infections or those that establish long‐term colonisations. The objective of this open, prospective pilot study was to quantitatively and qualitatively assess the microbiological status of ‘locally infected’ venous leg ulcers. Three different sampling techniques were used to recover organisms from 20 venous leg ulcers that had failed to progress towards healing and comparisons made to explore the distribution of colonised flora within the wound. In total, 116 isolates were recovered (mean of 5·8 per ulcer) with highest recovery rates seen with swabs. Greatest agreement of colonisation and counts was found between swabs and absorbent polyvinyl acetate (PVA) foam disc, and also between PVA discs and biopsies. Lowest agreement was between the distribution of anaerobes and coryneforms in swabs and biopsies, suggesting uneven vertical distribution within ulcers. No justification for using routine biopsies in locally infected venous leg ulcers was found.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Infections - microbiology</subject><subject>Bacterial Infections - pathology</subject><subject>Bioburden</subject><subject>Biopsy</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>Humans</subject><subject>Leg Ulcer - microbiology</subject><subject>Leg Ulcer - pathology</subject><subject>Localised wound infection</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Venous leg ulcers</subject><subject>Wound Infection - microbiology</subject><subject>Wound Infection - pathology</subject><subject>Wound swabs</subject><issn>1742-4801</issn><issn>1742-481X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUV1v1DAQtBCIlsJfQH67pwTbieNEQkil0FJUgYQqtfCycnybqw9f3MZOufv3OL0SwRv74o-dmV3NEEI5y3mqN-ucq1JkZc2vc8FYkzNWlSzfPiGHc-PpfGf8gLwIYc2YaKRUz8lB4kjOGnVIvh9T42xvjXbU9vcYol3paH2fXtHTeIN0Y83gW-udXz3AQtRxDNR3dOF8-nC7hO3QRFwuqMMVHZ3BIbwkzzrtAr56PI_I5enHy5NP2cXXs_OT44vMpBVYtiyEqtuSKzRGCClb0abtZSW6jglsFXaN4ao2TVWXTBiFbSpVFK2SdYF1cUTe7WVvx3aDS4N9HLSD28Fu9LADry382-ntDaz8PahGcqkmgcWjwODvxmQAbGww6Jzu0Y8B0izFm4qzhKz3yORHCAN28xTOYMoF1jBZDpP9MOUCD7nANlFf_73lTPwTRAK83QN-WYe7_xaG86vP6ZLo2Z5uQ8TtTNfDT6hUoSRcfTmDH9eV4h--vYfT4jcp7q0U</recordid><startdate>200912</startdate><enddate>200912</enddate><creator>Cooper, Rose A</creator><creator>Ameen, Hanar</creator><creator>Price, Patricia</creator><creator>McCulloch, Dorothy A</creator><creator>Harding, Keith G</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200912</creationdate><title>A clinical investigation into the microbiological status of 'locally infected' leg ulcers</title><author>Cooper, Rose A ; Ameen, Hanar ; Price, Patricia ; McCulloch, Dorothy A ; Harding, Keith G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5100-d3278b417ecc2255b2b481562ff02eb7ef9c178c968402c7ebbbb733b7583e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Infections - microbiology</topic><topic>Bacterial Infections - pathology</topic><topic>Bioburden</topic><topic>Biopsy</topic><topic>Diagnosis, Differential</topic><topic>Female</topic><topic>Humans</topic><topic>Leg Ulcer - microbiology</topic><topic>Leg Ulcer - pathology</topic><topic>Localised wound infection</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Venous leg ulcers</topic><topic>Wound Infection - microbiology</topic><topic>Wound Infection - pathology</topic><topic>Wound swabs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cooper, Rose A</creatorcontrib><creatorcontrib>Ameen, Hanar</creatorcontrib><creatorcontrib>Price, Patricia</creatorcontrib><creatorcontrib>McCulloch, Dorothy A</creatorcontrib><creatorcontrib>Harding, Keith G</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International wound journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Cooper, Rose A</au><au>Ameen, Hanar</au><au>Price, Patricia</au><au>McCulloch, Dorothy A</au><au>Harding, Keith G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A clinical investigation into the microbiological status of 'locally infected' leg ulcers</atitle><jtitle>International wound journal</jtitle><addtitle>Int Wound J</addtitle><date>2009-12</date><risdate>2009</risdate><volume>6</volume><issue>6</issue><spage>453</spage><epage>462</epage><pages>453-462</pages><issn>1742-4801</issn><eissn>1742-481X</eissn><abstract>Cooper RA, Ameen H, Price P, McCulloch DA, Harding KG. A clinical investigation into the microbiological status of ‘locally infected’ leg ulcers
The complex interactions between patients and the microbial species that reside in their wounds are not yet fully characterised. Investigations to date have dealt with either those organisms that cause infections or those that establish long‐term colonisations. The objective of this open, prospective pilot study was to quantitatively and qualitatively assess the microbiological status of ‘locally infected’ venous leg ulcers. Three different sampling techniques were used to recover organisms from 20 venous leg ulcers that had failed to progress towards healing and comparisons made to explore the distribution of colonised flora within the wound. In total, 116 isolates were recovered (mean of 5·8 per ulcer) with highest recovery rates seen with swabs. Greatest agreement of colonisation and counts was found between swabs and absorbent polyvinyl acetate (PVA) foam disc, and also between PVA discs and biopsies. Lowest agreement was between the distribution of anaerobes and coryneforms in swabs and biopsies, suggesting uneven vertical distribution within ulcers. No justification for using routine biopsies in locally infected venous leg ulcers was found.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20051097</pmid><doi>10.1111/j.1742-481X.2009.00640.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Aged, 80 and over Bacteria - isolation & purification Bacterial Infections - microbiology Bacterial Infections - pathology Bioburden Biopsy Diagnosis, Differential Female Humans Leg Ulcer - microbiology Leg Ulcer - pathology Localised wound infection Male Middle Aged Original Venous leg ulcers Wound Infection - microbiology Wound Infection - pathology Wound swabs |
| title | A clinical investigation into the microbiological status of 'locally infected' leg ulcers |
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