SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells
Novel biomaterials can be used to provide a better environment for cross talk between vessel forming endothelial cells and wound healing instructor stem cells for tissue regeneration. This study seeks to investigate if a collagen scaffold containing a proangiogenic gene encoding for the chemokine st...
Gespeichert in:
| Veröffentlicht in: | Journal of materials science. Materials in medicine 2021-03, Vol.32 (3), p.26-26, Article 26 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 26 |
|---|---|
| container_issue | 3 |
| container_start_page | 26 |
| container_title | Journal of materials science. Materials in medicine |
| container_volume | 32 |
| creator | Laiva, Ashang L. O’Brien, Fergal J. Keogh, Michael B. |
| description | Novel biomaterials can be used to provide a better environment for cross talk between vessel forming endothelial cells and wound healing instructor stem cells for tissue regeneration. This study seeks to investigate if a collagen scaffold containing a proangiogenic gene encoding for the chemokine stromal-derived factor-1 alpha (SDF-1α GAS) could be used to enhance functional responses in a coculture of human umbilical vein endothelial cells (HUVECs) and human adipose-derived stem/stromal cells (ADSCs). Functional responses were determined by (1) monitoring the amount of junctional adhesion molecule VE-cadherin released during 14 days culture, (2) expression of provasculogenic genes on the 14th day, and (3) the bioactivity of secreted factors on neurogenic human Schwann cells. When we compared our SDF-1α GAS with a gene-free scaffold, the results showed positive proangiogenic determination characterized by a transient yet controlled release of the VE-cadherin. On the 14th day, the coculture on the SDF-1α GAS showed enhanced maturation than its gene-free equivalent through the elevation of provasculogenic genes (SDF-1α—7.4-fold, CXCR4—1.5-fold, eNOS—1.5-fold). Furthermore, we also found that the coculture on SDF-1α GAS secretes bioactive factors that significantly (
p
|
| doi_str_mv | 10.1007/s10856-021-06499-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7936958</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2498990877</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c8566c4c429086d46b9908166db6ddeb0a1572a5b5ecc775befa1e5a89dac8e53</originalsourceid><addsrcrecordid>eNp9Uctu1DAUjRCIDoUfYIEssWFjsBM_4g0SKhSQKrEA1pZj30xcJXawk6n4GD6CH-k31cNMy2PBypbP497jU1VPKXlJCZGvMiUtF5jUFBPBlMLiXrWhXDaYtU17v9oQxSVmvCEn1aOcLwkhTHH-sDppGiGl5HRT_fj89hzT659oCwGwsYvfmQUcsnEcTXlD2Zq-j6NDEAYTLGQ0p7gz2a5jLLi3KEGeY8iAfECmCAuyrAlQ7NGwTiYUpYvLAKM3I7Iwjhld-WU4gsb5OWbADpLflcF5SXG6JT6uHvRmzPDkeJ5WX8_ffTn7gC8-vf949uYCWybZgm35BmGZZbUirXBMdKpcqBCuE85BR0z5ldrwjoO1JXcHvaHATaucsS3w5rR6ffCd124CZyEsyYx6Tn4y6buOxuu_keAHvY07LVUjFG-LwYujQYrfVsiLnnzeRzAB4pp1zVS730nKQn3-D_UyrimUeHuWFFRxygqrPrBsijkn6O-WoUTv29eH9nVpX_9qX4sievZnjDvJbd2F0BwIuUBhC-n37P_Y3gDRosBW</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2497619514</pqid></control><display><type>article</type><title>SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells</title><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>Laiva, Ashang L. ; O’Brien, Fergal J. ; Keogh, Michael B.</creator><creatorcontrib>Laiva, Ashang L. ; O’Brien, Fergal J. ; Keogh, Michael B.</creatorcontrib><description>Novel biomaterials can be used to provide a better environment for cross talk between vessel forming endothelial cells and wound healing instructor stem cells for tissue regeneration. This study seeks to investigate if a collagen scaffold containing a proangiogenic gene encoding for the chemokine stromal-derived factor-1 alpha (SDF-1α GAS) could be used to enhance functional responses in a coculture of human umbilical vein endothelial cells (HUVECs) and human adipose-derived stem/stromal cells (ADSCs). Functional responses were determined by (1) monitoring the amount of junctional adhesion molecule VE-cadherin released during 14 days culture, (2) expression of provasculogenic genes on the 14th day, and (3) the bioactivity of secreted factors on neurogenic human Schwann cells. When we compared our SDF-1α GAS with a gene-free scaffold, the results showed positive proangiogenic determination characterized by a transient yet controlled release of the VE-cadherin. On the 14th day, the coculture on the SDF-1α GAS showed enhanced maturation than its gene-free equivalent through the elevation of provasculogenic genes (SDF-1α—7.4-fold, CXCR4—1.5-fold, eNOS—1.5-fold). Furthermore, we also found that the coculture on SDF-1α GAS secretes bioactive factors that significantly (
p
< 0.01) enhanced human Schwann cells’ clustering to develop toward Bünger band-like structures. Conclusively, this study reports that SDF-1α GAS could be used to produce a bioactive vascularized construct through the enhancement of the cooperative effects between endothelial cells and ADSCs.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-021-06499-6</identifier><identifier>PMID: 33677751</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biological activity ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Cadherins ; Cell culture ; Ceramics ; Chemistry and Materials Science ; Chemokines ; Clustering ; Collagen ; Composites ; Controlled release ; Crosstalk ; CXCR4 protein ; Endothelial cells ; Gene expression ; Genes ; Glass ; Materials Science ; Natural Materials ; Nitric oxide ; Polymer Sciences ; Regeneration ; Regenerative Medicine/Tissue Engineering ; Scaffolds ; Schwann cells ; SDF-1 protein ; Stem cells ; Stromal cells ; Surfaces and Interfaces ; Thin Films ; Tissue engineering ; Tissue Engineering Constructs and Cell Substrates ; Umbilical vein ; Wound healing</subject><ispartof>Journal of materials science. Materials in medicine, 2021-03, Vol.32 (3), p.26-26, Article 26</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c8566c4c429086d46b9908166db6ddeb0a1572a5b5ecc775befa1e5a89dac8e53</citedby><cites>FETCH-LOGICAL-c474t-c8566c4c429086d46b9908166db6ddeb0a1572a5b5ecc775befa1e5a89dac8e53</cites><orcidid>0000-0003-3810-8044</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-021-06499-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10856-021-06499-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,864,885,27924,27925,41120,41488,42189,42557,51319,51576</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33677751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laiva, Ashang L.</creatorcontrib><creatorcontrib>O’Brien, Fergal J.</creatorcontrib><creatorcontrib>Keogh, Michael B.</creatorcontrib><title>SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Novel biomaterials can be used to provide a better environment for cross talk between vessel forming endothelial cells and wound healing instructor stem cells for tissue regeneration. This study seeks to investigate if a collagen scaffold containing a proangiogenic gene encoding for the chemokine stromal-derived factor-1 alpha (SDF-1α GAS) could be used to enhance functional responses in a coculture of human umbilical vein endothelial cells (HUVECs) and human adipose-derived stem/stromal cells (ADSCs). Functional responses were determined by (1) monitoring the amount of junctional adhesion molecule VE-cadherin released during 14 days culture, (2) expression of provasculogenic genes on the 14th day, and (3) the bioactivity of secreted factors on neurogenic human Schwann cells. When we compared our SDF-1α GAS with a gene-free scaffold, the results showed positive proangiogenic determination characterized by a transient yet controlled release of the VE-cadherin. On the 14th day, the coculture on the SDF-1α GAS showed enhanced maturation than its gene-free equivalent through the elevation of provasculogenic genes (SDF-1α—7.4-fold, CXCR4—1.5-fold, eNOS—1.5-fold). Furthermore, we also found that the coculture on SDF-1α GAS secretes bioactive factors that significantly (
p
< 0.01) enhanced human Schwann cells’ clustering to develop toward Bünger band-like structures. Conclusively, this study reports that SDF-1α GAS could be used to produce a bioactive vascularized construct through the enhancement of the cooperative effects between endothelial cells and ADSCs.</description><subject>Biological activity</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Cadherins</subject><subject>Cell culture</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Chemokines</subject><subject>Clustering</subject><subject>Collagen</subject><subject>Composites</subject><subject>Controlled release</subject><subject>Crosstalk</subject><subject>CXCR4 protein</subject><subject>Endothelial cells</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glass</subject><subject>Materials Science</subject><subject>Natural Materials</subject><subject>Nitric oxide</subject><subject>Polymer Sciences</subject><subject>Regeneration</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Scaffolds</subject><subject>Schwann cells</subject><subject>SDF-1 protein</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><subject>Tissue engineering</subject><subject>Tissue Engineering Constructs and Cell Substrates</subject><subject>Umbilical vein</subject><subject>Wound healing</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9Uctu1DAUjRCIDoUfYIEssWFjsBM_4g0SKhSQKrEA1pZj30xcJXawk6n4GD6CH-k31cNMy2PBypbP497jU1VPKXlJCZGvMiUtF5jUFBPBlMLiXrWhXDaYtU17v9oQxSVmvCEn1aOcLwkhTHH-sDppGiGl5HRT_fj89hzT659oCwGwsYvfmQUcsnEcTXlD2Zq-j6NDEAYTLGQ0p7gz2a5jLLi3KEGeY8iAfECmCAuyrAlQ7NGwTiYUpYvLAKM3I7Iwjhld-WU4gsb5OWbADpLflcF5SXG6JT6uHvRmzPDkeJ5WX8_ffTn7gC8-vf949uYCWybZgm35BmGZZbUirXBMdKpcqBCuE85BR0z5ldrwjoO1JXcHvaHATaucsS3w5rR6ffCd124CZyEsyYx6Tn4y6buOxuu_keAHvY07LVUjFG-LwYujQYrfVsiLnnzeRzAB4pp1zVS730nKQn3-D_UyrimUeHuWFFRxygqrPrBsijkn6O-WoUTv29eH9nVpX_9qX4sievZnjDvJbd2F0BwIuUBhC-n37P_Y3gDRosBW</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Laiva, Ashang L.</creator><creator>O’Brien, Fergal J.</creator><creator>Keogh, Michael B.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0W</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3810-8044</orcidid></search><sort><creationdate>20210301</creationdate><title>SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells</title><author>Laiva, Ashang L. ; O’Brien, Fergal J. ; Keogh, Michael B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c8566c4c429086d46b9908166db6ddeb0a1572a5b5ecc775befa1e5a89dac8e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biological activity</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Cadherins</topic><topic>Cell culture</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Chemokines</topic><topic>Clustering</topic><topic>Collagen</topic><topic>Composites</topic><topic>Controlled release</topic><topic>Crosstalk</topic><topic>CXCR4 protein</topic><topic>Endothelial cells</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Glass</topic><topic>Materials Science</topic><topic>Natural Materials</topic><topic>Nitric oxide</topic><topic>Polymer Sciences</topic><topic>Regeneration</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Scaffolds</topic><topic>Schwann cells</topic><topic>SDF-1 protein</topic><topic>Stem cells</topic><topic>Stromal cells</topic><topic>Surfaces and Interfaces</topic><topic>Thin Films</topic><topic>Tissue engineering</topic><topic>Tissue Engineering Constructs and Cell Substrates</topic><topic>Umbilical vein</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laiva, Ashang L.</creatorcontrib><creatorcontrib>O’Brien, Fergal J.</creatorcontrib><creatorcontrib>Keogh, Michael B.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>DELNET Engineering & Technology Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of materials science. Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laiva, Ashang L.</au><au>O’Brien, Fergal J.</au><au>Keogh, Michael B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>32</volume><issue>3</issue><spage>26</spage><epage>26</epage><pages>26-26</pages><artnum>26</artnum><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>Novel biomaterials can be used to provide a better environment for cross talk between vessel forming endothelial cells and wound healing instructor stem cells for tissue regeneration. This study seeks to investigate if a collagen scaffold containing a proangiogenic gene encoding for the chemokine stromal-derived factor-1 alpha (SDF-1α GAS) could be used to enhance functional responses in a coculture of human umbilical vein endothelial cells (HUVECs) and human adipose-derived stem/stromal cells (ADSCs). Functional responses were determined by (1) monitoring the amount of junctional adhesion molecule VE-cadherin released during 14 days culture, (2) expression of provasculogenic genes on the 14th day, and (3) the bioactivity of secreted factors on neurogenic human Schwann cells. When we compared our SDF-1α GAS with a gene-free scaffold, the results showed positive proangiogenic determination characterized by a transient yet controlled release of the VE-cadherin. On the 14th day, the coculture on the SDF-1α GAS showed enhanced maturation than its gene-free equivalent through the elevation of provasculogenic genes (SDF-1α—7.4-fold, CXCR4—1.5-fold, eNOS—1.5-fold). Furthermore, we also found that the coculture on SDF-1α GAS secretes bioactive factors that significantly (
p
< 0.01) enhanced human Schwann cells’ clustering to develop toward Bünger band-like structures. Conclusively, this study reports that SDF-1α GAS could be used to produce a bioactive vascularized construct through the enhancement of the cooperative effects between endothelial cells and ADSCs.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33677751</pmid><doi>10.1007/s10856-021-06499-6</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3810-8044</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0957-4530 |
| ispartof | Journal of materials science. Materials in medicine, 2021-03, Vol.32 (3), p.26-26, Article 26 |
| issn | 0957-4530 1573-4838 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7936958 |
| source | DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; SpringerLink Journals - AutoHoldings |
| subjects | Biological activity Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Cadherins Cell culture Ceramics Chemistry and Materials Science Chemokines Clustering Collagen Composites Controlled release Crosstalk CXCR4 protein Endothelial cells Gene expression Genes Glass Materials Science Natural Materials Nitric oxide Polymer Sciences Regeneration Regenerative Medicine/Tissue Engineering Scaffolds Schwann cells SDF-1 protein Stem cells Stromal cells Surfaces and Interfaces Thin Films Tissue engineering Tissue Engineering Constructs and Cell Substrates Umbilical vein Wound healing |
| title | SDF-1α gene-activated collagen scaffold enhances provasculogenic response in a coculture of human endothelial cells with human adipose-derived stromal cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T07%3A23%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=SDF-1%CE%B1%20gene-activated%20collagen%20scaffold%20enhances%20provasculogenic%20response%20in%20a%20coculture%20of%20human%20endothelial%20cells%20with%20human%20adipose-derived%20stromal%20cells&rft.jtitle=Journal%20of%20materials%20science.%20Materials%20in%20medicine&rft.au=Laiva,%20Ashang%20L.&rft.date=2021-03-01&rft.volume=32&rft.issue=3&rft.spage=26&rft.epage=26&rft.pages=26-26&rft.artnum=26&rft.issn=0957-4530&rft.eissn=1573-4838&rft_id=info:doi/10.1007/s10856-021-06499-6&rft_dat=%3Cproquest_pubme%3E2498990877%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2497619514&rft_id=info:pmid/33677751&rfr_iscdi=true |