Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus
The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of ra...
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| Veröffentlicht in: | The journal of physical chemistry letters 2021-03, Vol.12 (8), p.2166-2171 |
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| container_title | The journal of physical chemistry letters |
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| creator | Pramanik, Avijit Gao, Ye Patibandla, Shamily Mitra, Dipanwita McCandless, Martin G Fassero, Lauren A Gates, Kalein Tandon, Ritesh Ray, Paresh Chandra |
| description | The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer–spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus. |
| doi_str_mv | 10.1021/acs.jpclett.0c03570 |
| format | Article |
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Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer–spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.</description><identifier>ISSN: 1948-7185</identifier><identifier>EISSN: 1948-7185</identifier><identifier>DOI: 10.1021/acs.jpclett.0c03570</identifier><identifier>PMID: 33629859</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Angiotensin-Converting Enzyme 2 - metabolism ; Antigens, Viral - analysis ; Antigens, Viral - metabolism ; Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - metabolism ; Biosensing Techniques - methods ; COVID-19 - diagnosis ; COVID-19 Testing - methods ; Energy Transfer ; Gold - chemistry ; Humans ; Limit of Detection ; Metal Nanoparticles - chemistry ; Physical Insights into Materials and Molecular Properties ; Protein Binding ; SARS-CoV-2 - drug effects ; SARS-CoV-2 - metabolism ; Spike Glycoprotein, Coronavirus - analysis ; Spike Glycoprotein, Coronavirus - metabolism</subject><ispartof>The journal of physical chemistry letters, 2021-03, Vol.12 (8), p.2166-2171</ispartof><rights>2021 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a445t-e167707efdf050697831d3e82b9b7120e712e46ab8dafcf425184ee78e728dd33</citedby><cites>FETCH-LOGICAL-a445t-e167707efdf050697831d3e82b9b7120e712e46ab8dafcf425184ee78e728dd33</cites><orcidid>0000-0002-4413-0195 ; 0000-0002-4623-2099 ; 0000-0001-5398-9930</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.0c03570$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jpclett.0c03570$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33629859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pramanik, Avijit</creatorcontrib><creatorcontrib>Gao, Ye</creatorcontrib><creatorcontrib>Patibandla, Shamily</creatorcontrib><creatorcontrib>Mitra, Dipanwita</creatorcontrib><creatorcontrib>McCandless, Martin G</creatorcontrib><creatorcontrib>Fassero, Lauren A</creatorcontrib><creatorcontrib>Gates, Kalein</creatorcontrib><creatorcontrib>Tandon, Ritesh</creatorcontrib><creatorcontrib>Ray, Paresh Chandra</creatorcontrib><title>Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus</title><title>The journal of physical chemistry letters</title><addtitle>J. Phys. Chem. Lett</addtitle><description>The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer–spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.</description><subject>Angiotensin-Converting Enzyme 2 - metabolism</subject><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - metabolism</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Aptamers, Nucleotide - metabolism</subject><subject>Biosensing Techniques - methods</subject><subject>COVID-19 - diagnosis</subject><subject>COVID-19 Testing - methods</subject><subject>Energy Transfer</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Physical Insights into Materials and Molecular Properties</subject><subject>Protein Binding</subject><subject>SARS-CoV-2 - drug effects</subject><subject>SARS-CoV-2 - metabolism</subject><subject>Spike Glycoprotein, Coronavirus - analysis</subject><subject>Spike Glycoprotein, Coronavirus - metabolism</subject><issn>1948-7185</issn><issn>1948-7185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UdtuGyEURFWrJk37BZUqfmAd2IthXyqldppGitqHJH1Fx3BwsdawAtaSPyl_WWq7UfrSF-AcZoYzDCEfOZtxVvNL0Gm2GfWAOc-YZk0n2CtyzvtWVoLL7vWL8xl5l9KGsXnPpHhLzppmXvey68_J09WYYYuRLoLfTGvIaOhNGAz9Dj6kDLH6Aqn0lq4UXmO1xBG9QZ8PiBFidmUEej9FCxrptce43tOHCD7ZIns_os4xJB3GPbUh0schx6Lok8tuh3SJuQBc8BS8obceSrGDQyPYMlQMHuhPF6f0nryxMCT8cNovyOPX64fFt-rux83t4uqugrbtcoV8LgQTaI1lXTEsZMNNg7Je9SvBa4ZlwXYOK2nAatvWHZctopAoamlM01yQz0fdcVpt0ehiNcKgxui2EPcqgFP_3nj3S63DTom-LhKsCDRHAV18p4j2mcuZ-pOcKsmpU3LqlFxhfXr57DPnb1QFcHkEHNhhir78wn8lfwPrza4s</recordid><startdate>20210304</startdate><enddate>20210304</enddate><creator>Pramanik, Avijit</creator><creator>Gao, Ye</creator><creator>Patibandla, Shamily</creator><creator>Mitra, Dipanwita</creator><creator>McCandless, Martin G</creator><creator>Fassero, Lauren A</creator><creator>Gates, Kalein</creator><creator>Tandon, Ritesh</creator><creator>Ray, Paresh Chandra</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4413-0195</orcidid><orcidid>https://orcid.org/0000-0002-4623-2099</orcidid><orcidid>https://orcid.org/0000-0001-5398-9930</orcidid></search><sort><creationdate>20210304</creationdate><title>Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus</title><author>Pramanik, Avijit ; 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| ispartof | The journal of physical chemistry letters, 2021-03, Vol.12 (8), p.2166-2171 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Angiotensin-Converting Enzyme 2 - metabolism Antigens, Viral - analysis Antigens, Viral - metabolism Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Biosensing Techniques - methods COVID-19 - diagnosis COVID-19 Testing - methods Energy Transfer Gold - chemistry Humans Limit of Detection Metal Nanoparticles - chemistry Physical Insights into Materials and Molecular Properties Protein Binding SARS-CoV-2 - drug effects SARS-CoV-2 - metabolism Spike Glycoprotein, Coronavirus - analysis Spike Glycoprotein, Coronavirus - metabolism |
| title | Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus |
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