In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts
This study evaluated in vitro effect of different concentrations of Aloe vera ( A. vera ) ethanol extract and honey against Acanthamoeba spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of Acanthamoeba infection) at different incubation periods. Four different concentrat...
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| Veröffentlicht in: | Journal of parasitic diseases 2021-03, Vol.45 (1), p.159-168 |
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| creator | Kadry, Ghada Mohamed Ismail, Mousa A. M. El-Sayed, Nagwa Mostafa El-Kholy, Hanan S. El-Akkad, Dina M. Hamdy |
| description | This study evaluated in vitro effect of different concentrations of
Aloe vera
(
A. vera
) ethanol extract and honey against
Acanthamoeba
spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of
Acanthamoeba
infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 μg/ml for
A. vera ethanol extract
and 25, 50, 100, and 200 μg/ml for honey. Isolated
Acanthamoeba
spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of
A. vera
extract and honey against
Acanthamoeba
cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both
A. vera
ethanol extract and honey showed a concentration and time-dependent effect on the viability of
Acanthamoeba
cysts. In comparison with chlorhexidine (the drug control),
A. vera
ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 μg/ml which recorded the lower inhibitory effect. With increasing the dose of
A. vera
ethanol extract to 200, 400, 600 µg/ml, the recorded inhibitory percentages of
Acanthamoeba
cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50–100 µg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 µg/ml) gave an inhibitory effect by 47.7–67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 µg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope,
Acanthamoeba
cysts treated by
A. vera
ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes |
| doi_str_mv | 10.1007/s12639-020-01292-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7921262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2494911656</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3898-c6630cc76b49de0081f5d1b8157f33fd848229cb196f47d09de1467d95908cc33</originalsourceid><addsrcrecordid>eNp9kUlvHCEQhZGVKN7yB3yIkHLxpZ1iaZZLpJEVL5IlX-wzommYaasHJtAz8vz7YI-XJAefQLyvXlXxEDohcEYA5I9CqGC6AQoNEKppo_bQAWjZNiCBfXq-k0YSLfbRYSkPAG19V1_QPmOSCw7kAHXXEW-GKSdsl8l3gxt6O2IfgncTTgHPxuTxxmeL_bSwMVXtccq2ijb2eJGi32I7t0MsE545Gyv05GNxWa3OsNuWqRyjz8GOxX99OY_Q_cWvu_Or5ub28vp8dtM4prRqnBAMnJOi47r3AIqEtiedIq0MjIVecUWpdl1dJ3DZQ4UIF7LXrQblHGNH6OfOd7Xulr53PtZBR7PKw9LmrUl2MP8qcViYedoYqWn9SVoNTl8Mcvq99mUyy6E4P442-rQuhrbAhOBa8Yp-_w99SOsc63qGcs01IaIVlaI7yuVUSvbhbRgC5ilCs4vQ1AjNc4RG1aJvf6_xVvKaWQXYDihVinOf33t_YPsHHaOnAg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2494911656</pqid></control><display><type>article</type><title>In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts</title><source>Springer Nature - Complete Springer Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Kadry, Ghada Mohamed ; Ismail, Mousa A. M. ; El-Sayed, Nagwa Mostafa ; El-Kholy, Hanan S. ; El-Akkad, Dina M. Hamdy</creator><creatorcontrib>Kadry, Ghada Mohamed ; Ismail, Mousa A. M. ; El-Sayed, Nagwa Mostafa ; El-Kholy, Hanan S. ; El-Akkad, Dina M. Hamdy</creatorcontrib><description>This study evaluated in vitro effect of different concentrations of
Aloe vera
(
A. vera
) ethanol extract and honey against
Acanthamoeba
spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of
Acanthamoeba
infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 μg/ml for
A. vera ethanol extract
and 25, 50, 100, and 200 μg/ml for honey. Isolated
Acanthamoeba
spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of
A. vera
extract and honey against
Acanthamoeba
cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both
A. vera
ethanol extract and honey showed a concentration and time-dependent effect on the viability of
Acanthamoeba
cysts. In comparison with chlorhexidine (the drug control),
A. vera
ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 μg/ml which recorded the lower inhibitory effect. With increasing the dose of
A. vera
ethanol extract to 200, 400, 600 µg/ml, the recorded inhibitory percentages of
Acanthamoeba
cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50–100 µg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 µg/ml) gave an inhibitory effect by 47.7–67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 µg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope,
Acanthamoeba
cysts treated by
A. vera
ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes of
Acanthamoeba
cysts.
A. vera
ethanol extract and honey had considerable cysticidal effects on
Acanthamoeba
cysts. They may give promising results for treatment of
Acanthamoeba
keratitis.</description><identifier>ISSN: 0971-7196</identifier><identifier>EISSN: 0975-0703</identifier><identifier>DOI: 10.1007/s12639-020-01292-8</identifier><identifier>PMID: 33746401</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Acanthamoeba ; Aloe vera ; Chlorhexidine ; Cysts ; Drug dosages ; Ethanol ; Health Promotion and Disease Prevention ; Honey ; Infectious Diseases ; Keratitis ; Medicine ; Medicine & Public Health ; Morphology ; Original ; Original Article ; Scanning electron microscopy</subject><ispartof>Journal of parasitic diseases, 2021-03, Vol.45 (1), p.159-168</ispartof><rights>Indian Society for Parasitology 2020</rights><rights>Indian Society for Parasitology 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3898-c6630cc76b49de0081f5d1b8157f33fd848229cb196f47d09de1467d95908cc33</citedby><cites>FETCH-LOGICAL-c3898-c6630cc76b49de0081f5d1b8157f33fd848229cb196f47d09de1467d95908cc33</cites><orcidid>0000-0001-6880-2147</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921262/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921262/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33746401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kadry, Ghada Mohamed</creatorcontrib><creatorcontrib>Ismail, Mousa A. M.</creatorcontrib><creatorcontrib>El-Sayed, Nagwa Mostafa</creatorcontrib><creatorcontrib>El-Kholy, Hanan S.</creatorcontrib><creatorcontrib>El-Akkad, Dina M. Hamdy</creatorcontrib><title>In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts</title><title>Journal of parasitic diseases</title><addtitle>J Parasit Dis</addtitle><addtitle>J Parasit Dis</addtitle><description>This study evaluated in vitro effect of different concentrations of
Aloe vera
(
A. vera
) ethanol extract and honey against
Acanthamoeba
spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of
Acanthamoeba
infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 μg/ml for
A. vera ethanol extract
and 25, 50, 100, and 200 μg/ml for honey. Isolated
Acanthamoeba
spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of
A. vera
extract and honey against
Acanthamoeba
cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both
A. vera
ethanol extract and honey showed a concentration and time-dependent effect on the viability of
Acanthamoeba
cysts. In comparison with chlorhexidine (the drug control),
A. vera
ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 μg/ml which recorded the lower inhibitory effect. With increasing the dose of
A. vera
ethanol extract to 200, 400, 600 µg/ml, the recorded inhibitory percentages of
Acanthamoeba
cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50–100 µg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 µg/ml) gave an inhibitory effect by 47.7–67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 µg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope,
Acanthamoeba
cysts treated by
A. vera
ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes of
Acanthamoeba
cysts.
A. vera
ethanol extract and honey had considerable cysticidal effects on
Acanthamoeba
cysts. They may give promising results for treatment of
Acanthamoeba
keratitis.</description><subject>Acanthamoeba</subject><subject>Aloe vera</subject><subject>Chlorhexidine</subject><subject>Cysts</subject><subject>Drug dosages</subject><subject>Ethanol</subject><subject>Health Promotion and Disease Prevention</subject><subject>Honey</subject><subject>Infectious Diseases</subject><subject>Keratitis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Morphology</subject><subject>Original</subject><subject>Original Article</subject><subject>Scanning electron microscopy</subject><issn>0971-7196</issn><issn>0975-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kUlvHCEQhZGVKN7yB3yIkHLxpZ1iaZZLpJEVL5IlX-wzommYaasHJtAz8vz7YI-XJAefQLyvXlXxEDohcEYA5I9CqGC6AQoNEKppo_bQAWjZNiCBfXq-k0YSLfbRYSkPAG19V1_QPmOSCw7kAHXXEW-GKSdsl8l3gxt6O2IfgncTTgHPxuTxxmeL_bSwMVXtccq2ijb2eJGi32I7t0MsE545Gyv05GNxWa3OsNuWqRyjz8GOxX99OY_Q_cWvu_Or5ub28vp8dtM4prRqnBAMnJOi47r3AIqEtiedIq0MjIVecUWpdl1dJ3DZQ4UIF7LXrQblHGNH6OfOd7Xulr53PtZBR7PKw9LmrUl2MP8qcViYedoYqWn9SVoNTl8Mcvq99mUyy6E4P442-rQuhrbAhOBa8Yp-_w99SOsc63qGcs01IaIVlaI7yuVUSvbhbRgC5ilCs4vQ1AjNc4RG1aJvf6_xVvKaWQXYDihVinOf33t_YPsHHaOnAg</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Kadry, Ghada Mohamed</creator><creator>Ismail, Mousa A. M.</creator><creator>El-Sayed, Nagwa Mostafa</creator><creator>El-Kholy, Hanan S.</creator><creator>El-Akkad, Dina M. Hamdy</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6880-2147</orcidid></search><sort><creationdate>20210301</creationdate><title>In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts</title><author>Kadry, Ghada Mohamed ; Ismail, Mousa A. M. ; El-Sayed, Nagwa Mostafa ; El-Kholy, Hanan S. ; El-Akkad, Dina M. Hamdy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3898-c6630cc76b49de0081f5d1b8157f33fd848229cb196f47d09de1467d95908cc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acanthamoeba</topic><topic>Aloe vera</topic><topic>Chlorhexidine</topic><topic>Cysts</topic><topic>Drug dosages</topic><topic>Ethanol</topic><topic>Health Promotion and Disease Prevention</topic><topic>Honey</topic><topic>Infectious Diseases</topic><topic>Keratitis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Morphology</topic><topic>Original</topic><topic>Original Article</topic><topic>Scanning electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kadry, Ghada Mohamed</creatorcontrib><creatorcontrib>Ismail, Mousa A. M.</creatorcontrib><creatorcontrib>El-Sayed, Nagwa Mostafa</creatorcontrib><creatorcontrib>El-Kholy, Hanan S.</creatorcontrib><creatorcontrib>El-Akkad, Dina M. Hamdy</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of parasitic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kadry, Ghada Mohamed</au><au>Ismail, Mousa A. M.</au><au>El-Sayed, Nagwa Mostafa</au><au>El-Kholy, Hanan S.</au><au>El-Akkad, Dina M. Hamdy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts</atitle><jtitle>Journal of parasitic diseases</jtitle><stitle>J Parasit Dis</stitle><addtitle>J Parasit Dis</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>45</volume><issue>1</issue><spage>159</spage><epage>168</epage><pages>159-168</pages><issn>0971-7196</issn><eissn>0975-0703</eissn><abstract>This study evaluated in vitro effect of different concentrations of
Aloe vera
(
A. vera
) ethanol extract and honey against
Acanthamoeba
spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of
Acanthamoeba
infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 μg/ml for
A. vera ethanol extract
and 25, 50, 100, and 200 μg/ml for honey. Isolated
Acanthamoeba
spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of
A. vera
extract and honey against
Acanthamoeba
cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both
A. vera
ethanol extract and honey showed a concentration and time-dependent effect on the viability of
Acanthamoeba
cysts. In comparison with chlorhexidine (the drug control),
A. vera
ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 μg/ml which recorded the lower inhibitory effect. With increasing the dose of
A. vera
ethanol extract to 200, 400, 600 µg/ml, the recorded inhibitory percentages of
Acanthamoeba
cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50–100 µg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 µg/ml) gave an inhibitory effect by 47.7–67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 µg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope,
Acanthamoeba
cysts treated by
A. vera
ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes of
Acanthamoeba
cysts.
A. vera
ethanol extract and honey had considerable cysticidal effects on
Acanthamoeba
cysts. They may give promising results for treatment of
Acanthamoeba
keratitis.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>33746401</pmid><doi>10.1007/s12639-020-01292-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6880-2147</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of parasitic diseases, 2021-03, Vol.45 (1), p.159-168 |
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| source | Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acanthamoeba Aloe vera Chlorhexidine Cysts Drug dosages Ethanol Health Promotion and Disease Prevention Honey Infectious Diseases Keratitis Medicine Medicine & Public Health Morphology Original Original Article Scanning electron microscopy |
| title | In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts |
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