Variation of DNA Methylation in Newborns Associated with Exhaled Carbon Monoxide during Pregnancy
Fetal exposure to tobacco smoke is an adverse risk factor for newborns. A plausible mechanism of how this exposure may negatively impact long term health is differential methylation of deoxyribonucleic acid (DNAm) and its relation to birth weight. We examined whether self-reported gestational smokin...
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| Veröffentlicht in: | International journal of environmental research and public health 2021-02, Vol.18 (4), p.1597 |
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| creator | De Queiroz Andrade, Ediane Gomes, Gabriela Martins Costa Collison, Adam Grehan, Jane Murphy, Vanessa E Gibson, Peter Mattes, Joerg Karmaus, Wilfried |
| description | Fetal exposure to tobacco smoke is an adverse risk factor for newborns. A plausible mechanism of how this exposure may negatively impact long term health is differential methylation of deoxyribonucleic acid (DNAm) and its relation to birth weight. We examined whether self-reported gestational smoking status and maternal exhaled carbon monoxide (eCO) during early pregnancy were associated with methylation of cytosine by guanines (CpG) sites that themselves predicted birth weight. We focused first on CpGs associated with maternal smoking, and secondly, among these, on CpGs related to birth weight found in another cohort. Then in 94 newborns from the Breathing for Life Trial (BLT) DNAm levels in cord blood were determined using Infinium Methylation EPIC BeadChip measuring >850K CpGs. We regressed CpGs on eCO and tested via mediation analysis whether CpGs link eCO to birth weight. Nine smoking related CpG sites were significantly associated with birth weight. Among these nine CpGs the methylation of cg02264407 on the
gene was statistically significant and linked with eCO measurements. eCO greater than six ppm showed a 2.3% decrease in infant DNAm (
= 0.035) on the
gene. A 1% decrease in methylation at this site resulted in decreased birth weight by 44.8 g (
= 0.003). None of the nine CpGs tested was associated with self-reported smoking. This is the first study to report potential mediation of DNA methylation, linking eCO measurements during early pregnancy with birth weight. |
| doi_str_mv | 10.3390/ijerph18041597 |
| format | Article |
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gene was statistically significant and linked with eCO measurements. eCO greater than six ppm showed a 2.3% decrease in infant DNAm (
= 0.035) on the
gene. A 1% decrease in methylation at this site resulted in decreased birth weight by 44.8 g (
= 0.003). None of the nine CpGs tested was associated with self-reported smoking. This is the first study to report potential mediation of DNA methylation, linking eCO measurements during early pregnancy with birth weight.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18041597</identifier><identifier>PMID: 33567599</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Age ; Asthma ; Birth Weight ; Carbon Monoxide ; Cord blood ; CpG islands ; Cytosine ; Deoxyribonucleic acid ; DNA ; DNA Methylation ; Epigenesis, Genetic ; Exposure ; Female ; Fetuses ; Gene expression ; Guanines ; Humans ; Infant, Newborn ; Maternal Exposure ; Mediation ; Neonates ; Newborn babies ; Pregnancy ; Prenatal exposure ; Risk analysis ; Risk factors ; Smoking ; Socioeconomic factors ; Software ; Statistical analysis ; Tobacco ; Womens health</subject><ispartof>International journal of environmental research and public health, 2021-02, Vol.18 (4), p.1597</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-8203cdb190888dc5511eba34ad2a89dcb9a46194776be4d6ee58419baa575913</citedby><cites>FETCH-LOGICAL-c418t-8203cdb190888dc5511eba34ad2a89dcb9a46194776be4d6ee58419baa575913</cites><orcidid>0000-0001-8956-2779 ; 0000-0003-2684-1492</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915220/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915220/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33567599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Queiroz Andrade, Ediane</creatorcontrib><creatorcontrib>Gomes, Gabriela Martins Costa</creatorcontrib><creatorcontrib>Collison, Adam</creatorcontrib><creatorcontrib>Grehan, Jane</creatorcontrib><creatorcontrib>Murphy, Vanessa E</creatorcontrib><creatorcontrib>Gibson, Peter</creatorcontrib><creatorcontrib>Mattes, Joerg</creatorcontrib><creatorcontrib>Karmaus, Wilfried</creatorcontrib><title>Variation of DNA Methylation in Newborns Associated with Exhaled Carbon Monoxide during Pregnancy</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Fetal exposure to tobacco smoke is an adverse risk factor for newborns. A plausible mechanism of how this exposure may negatively impact long term health is differential methylation of deoxyribonucleic acid (DNAm) and its relation to birth weight. We examined whether self-reported gestational smoking status and maternal exhaled carbon monoxide (eCO) during early pregnancy were associated with methylation of cytosine by guanines (CpG) sites that themselves predicted birth weight. We focused first on CpGs associated with maternal smoking, and secondly, among these, on CpGs related to birth weight found in another cohort. Then in 94 newborns from the Breathing for Life Trial (BLT) DNAm levels in cord blood were determined using Infinium Methylation EPIC BeadChip measuring >850K CpGs. We regressed CpGs on eCO and tested via mediation analysis whether CpGs link eCO to birth weight. Nine smoking related CpG sites were significantly associated with birth weight. Among these nine CpGs the methylation of cg02264407 on the
gene was statistically significant and linked with eCO measurements. eCO greater than six ppm showed a 2.3% decrease in infant DNAm (
= 0.035) on the
gene. A 1% decrease in methylation at this site resulted in decreased birth weight by 44.8 g (
= 0.003). None of the nine CpGs tested was associated with self-reported smoking. This is the first study to report potential mediation of DNA methylation, linking eCO measurements during early pregnancy with birth weight.</description><subject>Age</subject><subject>Asthma</subject><subject>Birth Weight</subject><subject>Carbon Monoxide</subject><subject>Cord blood</subject><subject>CpG islands</subject><subject>Cytosine</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>Exposure</subject><subject>Female</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Guanines</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Maternal Exposure</subject><subject>Mediation</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Pregnancy</subject><subject>Prenatal exposure</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Smoking</subject><subject>Socioeconomic factors</subject><subject>Software</subject><subject>Statistical analysis</subject><subject>Tobacco</subject><subject>Womens health</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkU1vGyEQhlGVqkncXnuskHLpxSkssAuXSpbzKSVpD1GviIWxF2sNLuwm8b8vlpMo6WlmmIcZXl6EvlJyypgiP_wK0qajknAqVPMBHdG6JlNeE3rwJj9ExzmvCGGS1-oTOmRM1I1Q6giZPyZ5M_gYcFzgs7sZvoWh2_b7Ix_wHTy2MYWMZzlHW1Bw-NEPHT5_6kxfirlJbUFvY4hP3gF2Y_JhiX8nWAYT7PYz-rgwfYYvz3GC7i_O7-dX05tfl9fz2c3UciqHqawIs66likgpnRWCUmgN48ZVRipnW2V4TRVvmroF7moAITlVrTGiKKFsgn7ux27Gdg3OQhiS6fUm-bVJWx2N1-87wXd6GR90o6ioyvIJ-v48IMW_I-RBr3220PcmQByzrriUu19rqoKe_Ieu4phCUbejFKkoE6xQp3vKpphzgsXrYyjRO_P0e_PKhW9vJbziL26xf2HTl18</recordid><startdate>20210208</startdate><enddate>20210208</enddate><creator>De Queiroz Andrade, Ediane</creator><creator>Gomes, Gabriela Martins Costa</creator><creator>Collison, Adam</creator><creator>Grehan, Jane</creator><creator>Murphy, Vanessa E</creator><creator>Gibson, Peter</creator><creator>Mattes, Joerg</creator><creator>Karmaus, Wilfried</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8956-2779</orcidid><orcidid>https://orcid.org/0000-0003-2684-1492</orcidid></search><sort><creationdate>20210208</creationdate><title>Variation of DNA Methylation in Newborns Associated with Exhaled Carbon Monoxide during Pregnancy</title><author>De Queiroz Andrade, Ediane ; 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A plausible mechanism of how this exposure may negatively impact long term health is differential methylation of deoxyribonucleic acid (DNAm) and its relation to birth weight. We examined whether self-reported gestational smoking status and maternal exhaled carbon monoxide (eCO) during early pregnancy were associated with methylation of cytosine by guanines (CpG) sites that themselves predicted birth weight. We focused first on CpGs associated with maternal smoking, and secondly, among these, on CpGs related to birth weight found in another cohort. Then in 94 newborns from the Breathing for Life Trial (BLT) DNAm levels in cord blood were determined using Infinium Methylation EPIC BeadChip measuring >850K CpGs. We regressed CpGs on eCO and tested via mediation analysis whether CpGs link eCO to birth weight. Nine smoking related CpG sites were significantly associated with birth weight. Among these nine CpGs the methylation of cg02264407 on the
gene was statistically significant and linked with eCO measurements. eCO greater than six ppm showed a 2.3% decrease in infant DNAm (
= 0.035) on the
gene. A 1% decrease in methylation at this site resulted in decreased birth weight by 44.8 g (
= 0.003). None of the nine CpGs tested was associated with self-reported smoking. This is the first study to report potential mediation of DNA methylation, linking eCO measurements during early pregnancy with birth weight.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33567599</pmid><doi>10.3390/ijerph18041597</doi><orcidid>https://orcid.org/0000-0001-8956-2779</orcidid><orcidid>https://orcid.org/0000-0003-2684-1492</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Age Asthma Birth Weight Carbon Monoxide Cord blood CpG islands Cytosine Deoxyribonucleic acid DNA DNA Methylation Epigenesis, Genetic Exposure Female Fetuses Gene expression Guanines Humans Infant, Newborn Maternal Exposure Mediation Neonates Newborn babies Pregnancy Prenatal exposure Risk analysis Risk factors Smoking Socioeconomic factors Software Statistical analysis Tobacco Womens health |
| title | Variation of DNA Methylation in Newborns Associated with Exhaled Carbon Monoxide during Pregnancy |
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