Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)
In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mec...
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| Veröffentlicht in: | Molecular reproduction and development 2021-01, Vol.88 (1), p.15-33 |
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| creator | Zhang, Peng‐fei Huang, Yu‐lin Fu, Qiang He, Weng‐tan Xiao, Kai Zhang, Ming |
| description | In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mechanisms. Protein phosphorylation and ubiquitination are major PTMs in the regulation of many biological processes. In the present study, to our knowledge, we report the first phosphoproteome and ubiquitylome of sperm collected from the caput, corpus, and cauda segments of the epididymis using liquid chromatography–mass spectrometry combined with affinity purification. In total, 647 phosphorylation sites in 294 proteins and 1063 ubiquitination sites in 446 proteins were characterized. Some of these proteins were associated with cellular developmental processes and energy metabolic pathways. Interestingly, 84 proteins were both phosphorylated and ubiquitinated, simultaneously. Some of these proteins were involved in, for example, spermatogenesis, reproduction, and spermatid development. Taken together, these data provide a theoretical basis for further functional analysis of phosphorylation and ubiquitination in epididymal sperm of buffalo and other mammals, and serve as an important resource for exploring the physiological mechanism underlying sperm maturation.
Our study investigated the phosphoproteome and ubiquitylome of buffalo epididymal sperm for the first time. Our research develops our understanding of the molecular mechanisms underlying sperm maturation in buffalo. |
| doi_str_mv | 10.1002/mrd.23432 |
| format | Article |
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Our study investigated the phosphoproteome and ubiquitylome of buffalo epididymal sperm for the first time. Our research develops our understanding of the molecular mechanisms underlying sperm maturation in buffalo.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.23432</identifier><identifier>PMID: 33140506</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; buffalo epididymis ; Buffaloes - metabolism ; Cells, Cultured ; Epididymis ; Epididymis - metabolism ; Fertilization ; Kinases ; Liquid chromatography ; Male ; Mass spectroscopy ; Maturation ; Metabolic pathways ; Phosphoproteins - metabolism ; Phosphorylation ; Phosphorylation - physiology ; Protein purification ; Proteins ; Proteome - metabolism ; proteomics ; Proteomics - methods ; Reproduction - physiology ; Sperm ; sperm maturation ; Sperm Maturation - physiology ; Spermatogenesis ; Spermatozoa - metabolism ; Ubiquitinated Proteins - metabolism ; Ubiquitination ; Ubiquitination - physiology</subject><ispartof>Molecular reproduction and development, 2021-01, Vol.88 (1), p.15-33</ispartof><rights>2020 The Authors. published by Wiley Periodicals LLC</rights><rights>2020 The Authors. Molecular Reproduction and Development published by Wiley Periodicals LLC.</rights><rights>2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4432-f99f7c544eaa4f908301a70fa4636c924b91d8252e15175449d90e9863d6839b3</citedby><cites>FETCH-LOGICAL-c4432-f99f7c544eaa4f908301a70fa4636c924b91d8252e15175449d90e9863d6839b3</cites><orcidid>0000-0002-7840-5038</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.23432$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.23432$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33140506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Peng‐fei</creatorcontrib><creatorcontrib>Huang, Yu‐lin</creatorcontrib><creatorcontrib>Fu, Qiang</creatorcontrib><creatorcontrib>He, Weng‐tan</creatorcontrib><creatorcontrib>Xiao, Kai</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><title>Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)</title><title>Molecular reproduction and development</title><addtitle>Mol Reprod Dev</addtitle><description>In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mechanisms. Protein phosphorylation and ubiquitination are major PTMs in the regulation of many biological processes. In the present study, to our knowledge, we report the first phosphoproteome and ubiquitylome of sperm collected from the caput, corpus, and cauda segments of the epididymis using liquid chromatography–mass spectrometry combined with affinity purification. In total, 647 phosphorylation sites in 294 proteins and 1063 ubiquitination sites in 446 proteins were characterized. Some of these proteins were associated with cellular developmental processes and energy metabolic pathways. Interestingly, 84 proteins were both phosphorylated and ubiquitinated, simultaneously. Some of these proteins were involved in, for example, spermatogenesis, reproduction, and spermatid development. Taken together, these data provide a theoretical basis for further functional analysis of phosphorylation and ubiquitination in epididymal sperm of buffalo and other mammals, and serve as an important resource for exploring the physiological mechanism underlying sperm maturation.
Our study investigated the phosphoproteome and ubiquitylome of buffalo epididymal sperm for the first time. Our research develops our understanding of the molecular mechanisms underlying sperm maturation in buffalo.</description><subject>Animals</subject><subject>buffalo epididymis</subject><subject>Buffaloes - metabolism</subject><subject>Cells, Cultured</subject><subject>Epididymis</subject><subject>Epididymis - metabolism</subject><subject>Fertilization</subject><subject>Kinases</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mass spectroscopy</subject><subject>Maturation</subject><subject>Metabolic pathways</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation</subject><subject>Phosphorylation - physiology</subject><subject>Protein purification</subject><subject>Proteins</subject><subject>Proteome - metabolism</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>Reproduction - physiology</subject><subject>Sperm</subject><subject>sperm maturation</subject><subject>Sperm Maturation - physiology</subject><subject>Spermatogenesis</subject><subject>Spermatozoa - metabolism</subject><subject>Ubiquitinated Proteins - metabolism</subject><subject>Ubiquitination</subject><subject>Ubiquitination - physiology</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kV1r1zAUxoMobk4v_AJS8Ga76Ja3NsmNMOfbYCKIgnchbU73z0ibLmmUfntT_3Oo4EU44Ty_PJycB6HnBJ8SjOnZGO0pZZzRB-iQYCVrKlTzcLtzXPOGfjtAT1K6wRgrJfFjdMAY4bjB7SHaXU4LXEezgK3MZPyaXKrCUM27kMqZY1ggjFA0W-XO3Wa3rH5ruKmC2Vln19H4Ks0Qx-1dl4fB-FAdv86d8TmVRqkunTxFj4qQ4NldPUJf3739cvGhvvr0_vLi_KrueflAPSg1iL7hHIzhg8KSYWIEHgxvWdsryjtFrKQNBdIQUThlFQYlW2ZbyVTHjtCrve-cuxFsD9MSjddzdKOJqw7G6b-Vye30dfiuhVRlVbwYHN8ZxHCbIS16dKkH780EISdNeSOoEES0BX35D3oTcixb3ChJpGJc0UKd7Kk-hpQiDPfDEKy3_HTJT__Kr7Av_pz-nvwdWAHO9sAP52H9v5P--PnN3vIn8IWldw</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Zhang, Peng‐fei</creator><creator>Huang, Yu‐lin</creator><creator>Fu, Qiang</creator><creator>He, Weng‐tan</creator><creator>Xiao, Kai</creator><creator>Zhang, Ming</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7840-5038</orcidid></search><sort><creationdate>202101</creationdate><title>Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)</title><author>Zhang, Peng‐fei ; Huang, Yu‐lin ; Fu, Qiang ; He, Weng‐tan ; Xiao, Kai ; Zhang, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4432-f99f7c544eaa4f908301a70fa4636c924b91d8252e15175449d90e9863d6839b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>buffalo epididymis</topic><topic>Buffaloes - metabolism</topic><topic>Cells, Cultured</topic><topic>Epididymis</topic><topic>Epididymis - metabolism</topic><topic>Fertilization</topic><topic>Kinases</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Mass spectroscopy</topic><topic>Maturation</topic><topic>Metabolic pathways</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation</topic><topic>Phosphorylation - physiology</topic><topic>Protein purification</topic><topic>Proteins</topic><topic>Proteome - metabolism</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>Reproduction - physiology</topic><topic>Sperm</topic><topic>sperm maturation</topic><topic>Sperm Maturation - physiology</topic><topic>Spermatogenesis</topic><topic>Spermatozoa - metabolism</topic><topic>Ubiquitinated Proteins - metabolism</topic><topic>Ubiquitination</topic><topic>Ubiquitination - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Peng‐fei</creatorcontrib><creatorcontrib>Huang, Yu‐lin</creatorcontrib><creatorcontrib>Fu, Qiang</creatorcontrib><creatorcontrib>He, Weng‐tan</creatorcontrib><creatorcontrib>Xiao, Kai</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Peng‐fei</au><au>Huang, Yu‐lin</au><au>Fu, Qiang</au><au>He, Weng‐tan</au><au>Xiao, Kai</au><au>Zhang, Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis)</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol Reprod Dev</addtitle><date>2021-01</date><risdate>2021</risdate><volume>88</volume><issue>1</issue><spage>15</spage><epage>33</epage><pages>15-33</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><abstract>In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mechanisms. Protein phosphorylation and ubiquitination are major PTMs in the regulation of many biological processes. In the present study, to our knowledge, we report the first phosphoproteome and ubiquitylome of sperm collected from the caput, corpus, and cauda segments of the epididymis using liquid chromatography–mass spectrometry combined with affinity purification. In total, 647 phosphorylation sites in 294 proteins and 1063 ubiquitination sites in 446 proteins were characterized. Some of these proteins were associated with cellular developmental processes and energy metabolic pathways. Interestingly, 84 proteins were both phosphorylated and ubiquitinated, simultaneously. Some of these proteins were involved in, for example, spermatogenesis, reproduction, and spermatid development. Taken together, these data provide a theoretical basis for further functional analysis of phosphorylation and ubiquitination in epididymal sperm of buffalo and other mammals, and serve as an important resource for exploring the physiological mechanism underlying sperm maturation.
Our study investigated the phosphoproteome and ubiquitylome of buffalo epididymal sperm for the first time. Our research develops our understanding of the molecular mechanisms underlying sperm maturation in buffalo.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33140506</pmid><doi>10.1002/mrd.23432</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-7840-5038</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular reproduction and development, 2021-01, Vol.88 (1), p.15-33 |
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| language | eng |
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| subjects | Animals buffalo epididymis Buffaloes - metabolism Cells, Cultured Epididymis Epididymis - metabolism Fertilization Kinases Liquid chromatography Male Mass spectroscopy Maturation Metabolic pathways Phosphoproteins - metabolism Phosphorylation Phosphorylation - physiology Protein purification Proteins Proteome - metabolism proteomics Proteomics - methods Reproduction - physiology Sperm sperm maturation Sperm Maturation - physiology Spermatogenesis Spermatozoa - metabolism Ubiquitinated Proteins - metabolism Ubiquitination Ubiquitination - physiology |
| title | Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis) |
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