The histone demethylase KDM2B regulates human primordial germ cell-like cells specification
Germline specification is a fundamental step for human reproduction and this biological phenomenon possesses technical challenges to study as it occurs immediately after blastocyst implantation. The establishment of human primordial germ cell-like cells (hPGCLCs) induction system allows sophisticate...
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| Veröffentlicht in: | International journal of biological sciences 2021, Vol.17 (2), p.527-538 |
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| creator | Yuan, Weiyan Yao, Zhaokai Veerapandian, Veeramohan Yang, Xinyan Wang, Xiaoman Chen, Dingyao Ma, Linzi Li, Chaohui Zheng, Yi Luo, Fang Zhao, Xiao-Yang |
| description | Germline specification is a fundamental step for human reproduction and this biological phenomenon possesses technical challenges to study
as it occurs immediately after blastocyst implantation. The establishment of
human primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization of human primordial germ cells (hPGCs) development. However, the underlying molecular mechanisms of hPGCLC specification are not fully elucidated. Here, we observed particularly high expression of the histone demethylase
in male fetal germ cells (FGCs) but not in male somatic cells. Besides,
shared similar expression pattern with hPGC marker genes in hPGCLCs, suggesting an important role of
in germ cell development. Although deletion of
had no significant effects on human embryonic stem cell (hESC)'s pluripotency, loss of
dramatically impaired hPGCLCs differentiation whereas ectopically expressed KDM2B could efficiently rescue such defect, indicating this defect was due to
's loss in hPGCLC specification. Mechanistically, as revealed by the transcriptional profiling,
suppressed the expression of somatic genes thus inhibited somatic differentiation during hPGCLC specification. These data collectively indicate that KDM2B is an indispensable epigenetic regulator for hPGCLC specification, shedding lights on how epigenetic regulations orchestrate transcriptional events in hPGC development for future investigation. |
| doi_str_mv | 10.7150/ijbs.55873 |
| format | Article |
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as it occurs immediately after blastocyst implantation. The establishment of
human primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization of human primordial germ cells (hPGCs) development. However, the underlying molecular mechanisms of hPGCLC specification are not fully elucidated. Here, we observed particularly high expression of the histone demethylase
in male fetal germ cells (FGCs) but not in male somatic cells. Besides,
shared similar expression pattern with hPGC marker genes in hPGCLCs, suggesting an important role of
in germ cell development. Although deletion of
had no significant effects on human embryonic stem cell (hESC)'s pluripotency, loss of
dramatically impaired hPGCLCs differentiation whereas ectopically expressed KDM2B could efficiently rescue such defect, indicating this defect was due to
's loss in hPGCLC specification. Mechanistically, as revealed by the transcriptional profiling,
suppressed the expression of somatic genes thus inhibited somatic differentiation during hPGCLC specification. These data collectively indicate that KDM2B is an indispensable epigenetic regulator for hPGCLC specification, shedding lights on how epigenetic regulations orchestrate transcriptional events in hPGC development for future investigation.</description><identifier>ISSN: 1449-2288</identifier><identifier>EISSN: 1449-2288</identifier><identifier>DOI: 10.7150/ijbs.55873</identifier><identifier>PMID: 33613110</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher Pty Ltd</publisher><subject>Cell Differentiation - physiology ; Cell Lineage ; Cells, Cultured ; Clonal deletion ; Cloning ; Differentiation ; DNA methylation ; Embryogenesis ; Embryonic Stem Cells - cytology ; Epigenetics ; F-Box Proteins - genetics ; F-Box Proteins - physiology ; Fetuses ; Gene expression ; Gene Knockdown Techniques ; Genes ; Germ cells ; Germ Cells - cytology ; Histology ; Histones ; Humans ; In vivo methods and tests ; Jumonji Domain-Containing Histone Demethylases - genetics ; Jumonji Domain-Containing Histone Demethylases - physiology ; Males ; Molecular modelling ; Pluripotency ; Reproduction (biology) ; Research Paper ; Somatic cells ; Specifications ; Spermatogenesis ; Stem cells ; Transcription</subject><ispartof>International journal of biological sciences, 2021, Vol.17 (2), p.527-538</ispartof><rights>The author(s).</rights><rights>2021. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-e5d98f850e0757c1aed3891d99093a103e9509226744c8ff700a8f6fd38913a53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893587/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893587/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33613110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Weiyan</creatorcontrib><creatorcontrib>Yao, Zhaokai</creatorcontrib><creatorcontrib>Veerapandian, Veeramohan</creatorcontrib><creatorcontrib>Yang, Xinyan</creatorcontrib><creatorcontrib>Wang, Xiaoman</creatorcontrib><creatorcontrib>Chen, Dingyao</creatorcontrib><creatorcontrib>Ma, Linzi</creatorcontrib><creatorcontrib>Li, Chaohui</creatorcontrib><creatorcontrib>Zheng, Yi</creatorcontrib><creatorcontrib>Luo, Fang</creatorcontrib><creatorcontrib>Zhao, Xiao-Yang</creatorcontrib><title>The histone demethylase KDM2B regulates human primordial germ cell-like cells specification</title><title>International journal of biological sciences</title><addtitle>Int J Biol Sci</addtitle><description>Germline specification is a fundamental step for human reproduction and this biological phenomenon possesses technical challenges to study
as it occurs immediately after blastocyst implantation. The establishment of
human primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization of human primordial germ cells (hPGCs) development. However, the underlying molecular mechanisms of hPGCLC specification are not fully elucidated. Here, we observed particularly high expression of the histone demethylase
in male fetal germ cells (FGCs) but not in male somatic cells. Besides,
shared similar expression pattern with hPGC marker genes in hPGCLCs, suggesting an important role of
in germ cell development. Although deletion of
had no significant effects on human embryonic stem cell (hESC)'s pluripotency, loss of
dramatically impaired hPGCLCs differentiation whereas ectopically expressed KDM2B could efficiently rescue such defect, indicating this defect was due to
's loss in hPGCLC specification. Mechanistically, as revealed by the transcriptional profiling,
suppressed the expression of somatic genes thus inhibited somatic differentiation during hPGCLC specification. These data collectively indicate that KDM2B is an indispensable epigenetic regulator for hPGCLC specification, shedding lights on how epigenetic regulations orchestrate transcriptional events in hPGC development for future investigation.</description><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>Clonal deletion</subject><subject>Cloning</subject><subject>Differentiation</subject><subject>DNA methylation</subject><subject>Embryogenesis</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Epigenetics</subject><subject>F-Box Proteins - genetics</subject><subject>F-Box Proteins - physiology</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Genes</subject><subject>Germ cells</subject><subject>Germ Cells - cytology</subject><subject>Histology</subject><subject>Histones</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>Jumonji Domain-Containing Histone Demethylases - genetics</subject><subject>Jumonji Domain-Containing Histone Demethylases - physiology</subject><subject>Males</subject><subject>Molecular modelling</subject><subject>Pluripotency</subject><subject>Reproduction (biology)</subject><subject>Research Paper</subject><subject>Somatic cells</subject><subject>Specifications</subject><subject>Spermatogenesis</subject><subject>Stem cells</subject><subject>Transcription</subject><issn>1449-2288</issn><issn>1449-2288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkVtLxDAQhYMoXlZf_AES8EWErrk0bfIieFdUfNEnH0K2nW6zts2atIL_3nZdZRUCMzAfJ2fmILRPyTilgpzY2SSMhZApX0PbNI5VxJiU6yv9FtoJYUYIT4Qkm2iL84RySsk2en0uAZc2tK4BnEMNbflZmQD4_vKRnWMP064yLQRcdrVp8Nzb2vncmgpPwdc4g6qKKvsGiy7gMIfMFjYzrXXNLtooTBVgb1lH6OX66vniNnp4urm7OHuIspgkbQQiV7KQggBJRZpRAzmXiuZKEcUNJRyUIIqxJI3jTBZFSoiRRVIsKG4EH6HTb915N6khz6Bpvan04NX4T-2M1X8njS311H3oVCo-nG2EjpYC3r13EFpd2zAsZBpwXdAs7r-XjMW0Rw__oTPX-aZfTzOhJO8fGwSPv6nMuxA8FL9mKNFDZnrITC8y6-GDVfu_6E9I_AvbE5KR</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Yuan, Weiyan</creator><creator>Yao, Zhaokai</creator><creator>Veerapandian, Veeramohan</creator><creator>Yang, Xinyan</creator><creator>Wang, Xiaoman</creator><creator>Chen, Dingyao</creator><creator>Ma, Linzi</creator><creator>Li, Chaohui</creator><creator>Zheng, Yi</creator><creator>Luo, Fang</creator><creator>Zhao, Xiao-Yang</creator><general>Ivyspring International Publisher Pty Ltd</general><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2021</creationdate><title>The histone demethylase KDM2B regulates human primordial germ cell-like cells specification</title><author>Yuan, Weiyan ; 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as it occurs immediately after blastocyst implantation. The establishment of
human primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization of human primordial germ cells (hPGCs) development. However, the underlying molecular mechanisms of hPGCLC specification are not fully elucidated. Here, we observed particularly high expression of the histone demethylase
in male fetal germ cells (FGCs) but not in male somatic cells. Besides,
shared similar expression pattern with hPGC marker genes in hPGCLCs, suggesting an important role of
in germ cell development. Although deletion of
had no significant effects on human embryonic stem cell (hESC)'s pluripotency, loss of
dramatically impaired hPGCLCs differentiation whereas ectopically expressed KDM2B could efficiently rescue such defect, indicating this defect was due to
's loss in hPGCLC specification. Mechanistically, as revealed by the transcriptional profiling,
suppressed the expression of somatic genes thus inhibited somatic differentiation during hPGCLC specification. These data collectively indicate that KDM2B is an indispensable epigenetic regulator for hPGCLC specification, shedding lights on how epigenetic regulations orchestrate transcriptional events in hPGC development for future investigation.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher Pty Ltd</pub><pmid>33613110</pmid><doi>10.7150/ijbs.55873</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of biological sciences, 2021, Vol.17 (2), p.527-538 |
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| subjects | Cell Differentiation - physiology Cell Lineage Cells, Cultured Clonal deletion Cloning Differentiation DNA methylation Embryogenesis Embryonic Stem Cells - cytology Epigenetics F-Box Proteins - genetics F-Box Proteins - physiology Fetuses Gene expression Gene Knockdown Techniques Genes Germ cells Germ Cells - cytology Histology Histones Humans In vivo methods and tests Jumonji Domain-Containing Histone Demethylases - genetics Jumonji Domain-Containing Histone Demethylases - physiology Males Molecular modelling Pluripotency Reproduction (biology) Research Paper Somatic cells Specifications Spermatogenesis Stem cells Transcription |
| title | The histone demethylase KDM2B regulates human primordial germ cell-like cells specification |
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