Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing
Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2021, Vol.2021, p.6642606-8 |
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| creator | Kim, Ye-Ram Nam, Bomi Han, Ah-Reum Kim, Jin-Baek Jin, Chang Hyun |
| description | Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown that compounds from natural products have candidates for healing skin injury. Isoegomaketone (IK), isolated from leaves of Perilla frutescens var. crispa (Lamiaceae), has various bioactivities. However, the effect of IK on cutaneous wound healing processes has not been studied yet. In this study, we demonstrated that IK exhibits therapeutic wound healing effects using the human keratinocyte cell line HaCaT. Notably, IK promoted cell proliferation and migration in a dose-dependent manner in vitro, and treatment with 10 μM IK upregulated these processes by approximately 1.5-fold after 24 h compared with the control. IK induced the activation of the MAPK/ERK pathway and cell cycle progression to the S and G2/M phases. Thus, this study demonstrates IK as a potential candidate to upregulate wound healing that may provide therapeutic benefits to patients with delayed wound healing. |
| doi_str_mv | 10.1155/2021/6642606 |
| format | Article |
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Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing</title><source>Wiley-Blackwell Open Access Titles</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Kim, Ye-Ram ; Nam, Bomi ; Han, Ah-Reum ; Kim, Jin-Baek ; Jin, Chang Hyun</creator><contributor>Kek, Teh Ley ; Teh Ley Kek</contributor><creatorcontrib>Kim, Ye-Ram ; Nam, Bomi ; Han, Ah-Reum ; Kim, Jin-Baek ; Jin, Chang Hyun ; Kek, Teh Ley ; Teh Ley Kek</creatorcontrib><description>Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown that compounds from natural products have candidates for healing skin injury. Isoegomaketone (IK), isolated from leaves of Perilla frutescens var. crispa (Lamiaceae), has various bioactivities. However, the effect of IK on cutaneous wound healing processes has not been studied yet. In this study, we demonstrated that IK exhibits therapeutic wound healing effects using the human keratinocyte cell line HaCaT. Notably, IK promoted cell proliferation and migration in a dose-dependent manner in vitro, and treatment with 10 μM IK upregulated these processes by approximately 1.5-fold after 24 h compared with the control. IK induced the activation of the MAPK/ERK pathway and cell cycle progression to the S and G2/M phases. Thus, this study demonstrates IK as a potential candidate to upregulate wound healing that may provide therapeutic benefits to patients with delayed wound healing.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2021/6642606</identifier><identifier>PMID: 33628306</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Carbon dioxide ; Cell adhesion & migration ; Cell culture ; Cell cycle ; Cell growth ; Cell proliferation ; Cytokines ; Cytotoxicity ; Epidermis ; Extracellular signal-regulated kinase ; Fluorides ; Growth factors ; Keratinocytes ; Kinases ; MAP kinase ; Metabolic pathways ; Metabolism ; Natural products ; Perilla frutescens ; Proteins ; Skin ; Tumor necrosis factor-TNF ; Wound healing</subject><ispartof>Evidence-based complementary and alternative medicine, 2021, Vol.2021, p.6642606-8</ispartof><rights>Copyright © 2021 Ye-Ram Kim et al.</rights><rights>Copyright © 2021 Ye-Ram Kim et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Ye-Ram Kim et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-de74d36dfd7493c0f040c91e5c60de9e13440673e43cdb0de7c3e5b64a5ace313</citedby><cites>FETCH-LOGICAL-c448t-de74d36dfd7493c0f040c91e5c60de9e13440673e43cdb0de7c3e5b64a5ace313</cites><orcidid>0000-0001-5150-3060 ; 0000-0002-6604-8862 ; 0000-0001-7824-4441</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889401/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889401/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33628306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kek, Teh Ley</contributor><contributor>Teh Ley Kek</contributor><creatorcontrib>Kim, Ye-Ram</creatorcontrib><creatorcontrib>Nam, Bomi</creatorcontrib><creatorcontrib>Han, Ah-Reum</creatorcontrib><creatorcontrib>Kim, Jin-Baek</creatorcontrib><creatorcontrib>Jin, Chang Hyun</creatorcontrib><title>Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown that compounds from natural products have candidates for healing skin injury. Isoegomaketone (IK), isolated from leaves of Perilla frutescens var. crispa (Lamiaceae), has various bioactivities. However, the effect of IK on cutaneous wound healing processes has not been studied yet. In this study, we demonstrated that IK exhibits therapeutic wound healing effects using the human keratinocyte cell line HaCaT. Notably, IK promoted cell proliferation and migration in a dose-dependent manner in vitro, and treatment with 10 μM IK upregulated these processes by approximately 1.5-fold after 24 h compared with the control. IK induced the activation of the MAPK/ERK pathway and cell cycle progression to the S and G2/M phases. Thus, this study demonstrates IK as a potential candidate to upregulate wound healing that may provide therapeutic benefits to patients with delayed wound healing.</description><subject>Carbon dioxide</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Epidermis</subject><subject>Extracellular signal-regulated kinase</subject><subject>Fluorides</subject><subject>Growth factors</subject><subject>Keratinocytes</subject><subject>Kinases</subject><subject>MAP kinase</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Natural products</subject><subject>Perilla frutescens</subject><subject>Proteins</subject><subject>Skin</subject><subject>Tumor necrosis factor-TNF</subject><subject>Wound healing</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kc1vEzEQxVeIin7QG2dkiUsrmsZee-3dS6VSFVIliKgFwc1yvLOJ21272F6qHPnP8SohAg6cPJ756enNvCx7RfA5IUUxznFOxpyznGP-LDsggpERy8vy-a4W3_azwxDuMc4rIcSLbJ9SnpcU84Ps501wsHSdeoDoLKDGuw7NwZu2VenTRwgabEAns_NT9M6bGNFdNF3fqjRBHy_n0_H17RTNVVw9qTUyFk36Tlk0Ba-isU6vI6Do0DzpulTePSTkq-ttjSagWmOXL7O9RrUBjrfvUfbl_fXnq8lo9unDzdXlbKQZK-OoBsFqyuumFqyiGjeYYV0RKDTHNVRAKGOYCwqM6nqRWkJTKBacqUJpoIQeZRcb3cd-0UGdtopetfLRm075tXTKyL8n1qzk0v2QoiwrhgeBk62Ad997CFF2Jh0nHcqC64PMky_GKo5pQt_8g9673tu03kARUSSng-DZhtLeheCh2ZkhWA7ZyiFbuc024a__XGAH_w4zAW83wMrYWj2Z_8v9Aq24rOw</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Kim, Ye-Ram</creator><creator>Nam, Bomi</creator><creator>Han, Ah-Reum</creator><creator>Kim, Jin-Baek</creator><creator>Jin, Chang Hyun</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5150-3060</orcidid><orcidid>https://orcid.org/0000-0002-6604-8862</orcidid><orcidid>https://orcid.org/0000-0001-7824-4441</orcidid></search><sort><creationdate>2021</creationdate><title>Isoegomaketone from Perilla frutescens (L.) 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Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><spage>6642606</spage><epage>8</epage><pages>6642606-8</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown that compounds from natural products have candidates for healing skin injury. Isoegomaketone (IK), isolated from leaves of Perilla frutescens var. crispa (Lamiaceae), has various bioactivities. However, the effect of IK on cutaneous wound healing processes has not been studied yet. In this study, we demonstrated that IK exhibits therapeutic wound healing effects using the human keratinocyte cell line HaCaT. Notably, IK promoted cell proliferation and migration in a dose-dependent manner in vitro, and treatment with 10 μM IK upregulated these processes by approximately 1.5-fold after 24 h compared with the control. IK induced the activation of the MAPK/ERK pathway and cell cycle progression to the S and G2/M phases. Thus, this study demonstrates IK as a potential candidate to upregulate wound healing that may provide therapeutic benefits to patients with delayed wound healing.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>33628306</pmid><doi>10.1155/2021/6642606</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5150-3060</orcidid><orcidid>https://orcid.org/0000-0002-6604-8862</orcidid><orcidid>https://orcid.org/0000-0001-7824-4441</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Carbon dioxide Cell adhesion & migration Cell culture Cell cycle Cell growth Cell proliferation Cytokines Cytotoxicity Epidermis Extracellular signal-regulated kinase Fluorides Growth factors Keratinocytes Kinases MAP kinase Metabolic pathways Metabolism Natural products Perilla frutescens Proteins Skin Tumor necrosis factor-TNF Wound healing |
| title | Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing |
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