L-carnitine suppresses cisplatin-induced renal injury in rats: impact on cytoskeleton proteins expression

L-carnitine suppresses cisplatin-induced renal injury in rats: impact on cytoskeleton proteins expression

We designed this work to examine the curative role of L-carnitine (LCAR) in a rat model of cisplatin (CDDP)-induced kidney injury. We induced kidney injury in rats by a single intraperitoneal injection of 5 mg/kg of CDDP. Fifteen days post injection, rats were orally supplemented with 354 mg/kg of L...

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Veröffentlicht in:Toxicology research (Cambridge) 2021-01, Vol.10 (1), p.51-59
Hauptverfasser: Ebrahim, Osama Fouad Ahmed, Nafea, Ola Elsayed, Samy, Walaa, Shawky, Lamiaa Mohamed
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creator Ebrahim, Osama Fouad Ahmed
Nafea, Ola Elsayed
Samy, Walaa
Shawky, Lamiaa Mohamed
description We designed this work to examine the curative role of L-carnitine (LCAR) in a rat model of cisplatin (CDDP)-induced kidney injury. We induced kidney injury in rats by a single intraperitoneal injection of 5 mg/kg of CDDP. Fifteen days post injection, rats were orally supplemented with 354 mg/kg of LCAR for another 15 days. Kidney tissues were subjected to histo-biochemical analysis along with mRNA gene expression quantification for cytoskeleton proteins encoding genes (vimentin, nestin, and connexin 43) by real-time reverse transcription polymerase chain reaction. LCAR reversed CDDP-induced renal structural and functional impairments. LCAR significantly declined serum urea and creatinine concentrations, restored oxidant/antioxidant balance, reversed inflammation, and antagonized caspase 3-mediated apoptotic cell death in renal tissues. Moreover, LCAR effectively down-regulated cytoskeleton proteins mRNA levels, reflecting amelioration of CDDP-provoked podocyte injury. We concluded that LCAR has a favorable therapeutic utility against CDDP-induced kidney injury.
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title L-carnitine suppresses cisplatin-induced renal injury in rats: impact on cytoskeleton proteins expression
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