The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in spec...
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| Veröffentlicht in: | Plant biotechnology journal 2021-02, Vol.19 (2), p.221-223 |
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| creator | Li, Bo Liang, Sijia Alariqi, Muna Wang, Fuqiu Wang, Guanying Wang, Qiongqiong Xu, Zhongping Yu, Lu Naeem Zafar, Muhammad Sun, Lin Si, Huan Yuan, Daojun Guo, Weifeng Wang, Yanqin Lindsey, Keith Zhang, Xianlong Jin, Shuangxia |
| description | Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soya bean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. [...]we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding. With the increase of temperature, the bleaching phenotype of leaves becomes more obvious in T1 LbCpf1‐GhCLA1 transgenic plants grown in the field. (b) Phenotypes of T1 cotton plants with the target mutations in the GhCLA1 gene, grown at different temperatures. |
| doi_str_mv | 10.1111/pbi.13470 |
| format | Article |
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CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soya bean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. [...]we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding. With the increase of temperature, the bleaching phenotype of leaves becomes more obvious in T1 LbCpf1‐GhCLA1 transgenic plants grown in the field. (b) Phenotypes of T1 cotton plants with the target mutations in the GhCLA1 gene, grown at different temperatures.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/pbi.13470</identifier><identifier>PMID: 32854160</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Bleaching ; Brief Communication ; Brief Communications ; Clustered Regularly Interspaced Short Palindromic Repeats ; Cotton ; Cpf1 ; CRISPR ; CRISPR-Cas Systems - genetics ; Editing ; Efficiency ; Gene Editing ; Genome editing ; Genomes ; Genotype & phenotype ; Germplasm ; glandless ; Gossypium - genetics ; Gossypium hirsutum ; Gossypol ; gossypol‐free cotton ; Mutation ; nontransgenic ; Phenotypes ; Plant breeding ; Seeds ; Soybeans ; Temperature ; temperature sensitive ; Tobacco ; Transfer RNA ; Transgenic plants</subject><ispartof>Plant biotechnology journal, 2021-02, Vol.19 (2), p.221-223</ispartof><rights>2020 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4710-4b592cd7da81d44752cddd5b5676d92a89b95497d350a7f20793377fd032d0e03</citedby><cites>FETCH-LOGICAL-c4710-4b592cd7da81d44752cddd5b5676d92a89b95497d350a7f20793377fd032d0e03</cites><orcidid>0000-0002-9471-9168 ; 0000-0002-7703-524X ; 0000-0003-2559-9091 ; 0000-0002-1495-9154 ; 0000-0003-2103-6136 ; 0000-0001-5994-1882</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpbi.13470$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpbi.13470$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32854160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Liang, Sijia</creatorcontrib><creatorcontrib>Alariqi, Muna</creatorcontrib><creatorcontrib>Wang, Fuqiu</creatorcontrib><creatorcontrib>Wang, Guanying</creatorcontrib><creatorcontrib>Wang, Qiongqiong</creatorcontrib><creatorcontrib>Xu, Zhongping</creatorcontrib><creatorcontrib>Yu, Lu</creatorcontrib><creatorcontrib>Naeem Zafar, Muhammad</creatorcontrib><creatorcontrib>Sun, Lin</creatorcontrib><creatorcontrib>Si, Huan</creatorcontrib><creatorcontrib>Yuan, Daojun</creatorcontrib><creatorcontrib>Guo, Weifeng</creatorcontrib><creatorcontrib>Wang, Yanqin</creatorcontrib><creatorcontrib>Lindsey, Keith</creatorcontrib><creatorcontrib>Zhang, Xianlong</creatorcontrib><creatorcontrib>Jin, Shuangxia</creatorcontrib><title>The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soya bean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. [...]we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding. With the increase of temperature, the bleaching phenotype of leaves becomes more obvious in T1 LbCpf1‐GhCLA1 transgenic plants grown in the field. (b) Phenotypes of T1 cotton plants with the target mutations in the GhCLA1 gene, grown at different temperatures.</description><subject>Bleaching</subject><subject>Brief Communication</subject><subject>Brief Communications</subject><subject>Clustered Regularly Interspaced Short Palindromic Repeats</subject><subject>Cotton</subject><subject>Cpf1</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>Editing</subject><subject>Efficiency</subject><subject>Gene Editing</subject><subject>Genome editing</subject><subject>Genomes</subject><subject>Genotype & phenotype</subject><subject>Germplasm</subject><subject>glandless</subject><subject>Gossypium - genetics</subject><subject>Gossypium hirsutum</subject><subject>Gossypol</subject><subject>gossypol‐free cotton</subject><subject>Mutation</subject><subject>nontransgenic</subject><subject>Phenotypes</subject><subject>Plant breeding</subject><subject>Seeds</subject><subject>Soybeans</subject><subject>Temperature</subject><subject>temperature sensitive</subject><subject>Tobacco</subject><subject>Transfer RNA</subject><subject>Transgenic plants</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kkGO0zAUhiMEYobCggsgS2xaiba249jJBgkqGCpVYjQMa8uJX1qPUjvYzqDuOAJH4BpsOQonwaWlAiTw5vnJvz__z_qz7DHBM5LWvK_NjORM4DvZOWFcTAUv6N3TnrGz7EEINxhTwgt-PzvLaVkwwvF59vV6A0j1fWcaFY2zyLUowrYHr-LgAQWwwURza-IOLa6W7y6v5qt60bcEjVNVgVA1QVvQRkXQaA3WbQGlNhq7RsYi1XUuQvSq75zRqHExpkfGF7NvXzbGhyEO2wlSNp14OBmwzqYbNiScaZ6htQth17vu-6fPrQc4Qh5m91rVBXh0rKPs_etX14s309Xbi-XixWraMEHwlNVFRRsttCqJZkwUqdG6qAsuuK6oKqu6KlgldF5gJVqKRZXnQrQa51RjwPkoe37g9kOdBm1g762TvTdb5XfSKSP_PLFmI9fuVoqSl1WijbLxEeDdhwFClFsTGug6ZcENQVKWl8kMK2mSPv1LeuMGb9N4knJSEU6I4P9VsVLgSpT5njU5qBqf_s9De7JMsNzHRqbYyJ-xSdonv894Uv7KSRLMD4KPpoPdv0ny8uXygPwB98zP_g</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Li, Bo</creator><creator>Liang, Sijia</creator><creator>Alariqi, Muna</creator><creator>Wang, Fuqiu</creator><creator>Wang, Guanying</creator><creator>Wang, Qiongqiong</creator><creator>Xu, Zhongping</creator><creator>Yu, Lu</creator><creator>Naeem Zafar, Muhammad</creator><creator>Sun, Lin</creator><creator>Si, Huan</creator><creator>Yuan, Daojun</creator><creator>Guo, Weifeng</creator><creator>Wang, Yanqin</creator><creator>Lindsey, Keith</creator><creator>Zhang, Xianlong</creator><creator>Jin, Shuangxia</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9471-9168</orcidid><orcidid>https://orcid.org/0000-0002-7703-524X</orcidid><orcidid>https://orcid.org/0000-0003-2559-9091</orcidid><orcidid>https://orcid.org/0000-0002-1495-9154</orcidid><orcidid>https://orcid.org/0000-0003-2103-6136</orcidid><orcidid>https://orcid.org/0000-0001-5994-1882</orcidid></search><sort><creationdate>202102</creationdate><title>The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton</title><author>Li, Bo ; Liang, Sijia ; Alariqi, Muna ; Wang, Fuqiu ; Wang, Guanying ; Wang, Qiongqiong ; Xu, Zhongping ; Yu, Lu ; Naeem Zafar, Muhammad ; Sun, Lin ; Si, Huan ; Yuan, Daojun ; Guo, Weifeng ; Wang, Yanqin ; Lindsey, Keith ; Zhang, Xianlong ; Jin, Shuangxia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4710-4b592cd7da81d44752cddd5b5676d92a89b95497d350a7f20793377fd032d0e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bleaching</topic><topic>Brief Communication</topic><topic>Brief Communications</topic><topic>Clustered Regularly Interspaced Short Palindromic Repeats</topic><topic>Cotton</topic><topic>Cpf1</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems - 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CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al., 2019) and has been successfully applied in species such as rice, soya bean, tobacco, maize and cotton (Lee et al., 2019; Li et al., 2018; Tang et al., 2017; Xu et al., 2019). In order to improve LbCpf1 efficiency and determine the optimum temperature for cotton genome editing, we investigated the effects of different temperatures on LbCpf1 activity and genome editing efficiency. [...]we created nontransgenic and glandless cotton plants with seeds free of gossypol, representing a valuable germplasm resource for cotton breeding. With the increase of temperature, the bleaching phenotype of leaves becomes more obvious in T1 LbCpf1‐GhCLA1 transgenic plants grown in the field. (b) Phenotypes of T1 cotton plants with the target mutations in the GhCLA1 gene, grown at different temperatures.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>32854160</pmid><doi>10.1111/pbi.13470</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0002-9471-9168</orcidid><orcidid>https://orcid.org/0000-0002-7703-524X</orcidid><orcidid>https://orcid.org/0000-0003-2559-9091</orcidid><orcidid>https://orcid.org/0000-0002-1495-9154</orcidid><orcidid>https://orcid.org/0000-0003-2103-6136</orcidid><orcidid>https://orcid.org/0000-0001-5994-1882</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant biotechnology journal, 2021-02, Vol.19 (2), p.221-223 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Wiley-Blackwell Open Access Titles; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals |
| subjects | Bleaching Brief Communication Brief Communications Clustered Regularly Interspaced Short Palindromic Repeats Cotton Cpf1 CRISPR CRISPR-Cas Systems - genetics Editing Efficiency Gene Editing Genome editing Genomes Genotype & phenotype Germplasm glandless Gossypium - genetics Gossypium hirsutum Gossypol gossypol‐free cotton Mutation nontransgenic Phenotypes Plant breeding Seeds Soybeans Temperature temperature sensitive Tobacco Transfer RNA Transgenic plants |
| title | The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton |
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