Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls
The aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore ( Bos taurus indicus ) bulls were randomly allocated into three experimental groups: G1 ( n =12), a single 400 μg dose of anti-GnRH vaccine on day 0;...
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| Veröffentlicht in: | Tropical animal health and production 2021-03, Vol.53 (1), p.153-153, Article 153 |
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| creator | Doroteu, Emanuel M. Viana, Joao H. M. Ferreira Junior, Jair A. Macedo, Juliana T. A. Oliveira, Rodrigo A. Pedroso, Pedro M. O. |
| description | The aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (
Bos taurus indicus
) bulls were randomly allocated into three experimental groups: G1 (
n
=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (
n
=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG,
n
=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (
P
=0.0476), width (
P
=0.0021), and in scrotal circumference (
P
=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (
P |
| doi_str_mv | 10.1007/s11250-021-02600-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7867542</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2540476493</sourcerecordid><originalsourceid>FETCH-LOGICAL-c535t-7fcaefb032a4d3c2c5d59b5816f5ae755f82b03d54982f8915b2b8ea3a2157f13</originalsourceid><addsrcrecordid>eNqFks9vFCEcxYnR2HX1H_BgSLx4GeXnwFxMTFNbk0aTRs-EYWCXhoUVmNr-92W7tbUe2gTC4X3eI3x5ALzF6CNGSHwqGBOOOkRw2z1C3eUzsMBc0E4wKp-DBUJs6Jhg4gC8KuUcoWaT_UtwQClnYpBoAeYj56ypMDmoYfFxFSxMGdY_CU6p2HIjxLaq747j2Qm80Mb42KAIqy3Vm2bYpLxdp87N0VSfog7QrHXWptrsd0iBPsLvNqRs4TiHUF6DF06HYt_cnkvw6-vRz8OT7vTH8bfDL6ed4ZTXTjijrRsRJZpN1BDDJz6MXOLecW0F506Spk6cDZI4OWA-klFaTTVpY3CYLsHnfe52Hjd2MjbWrIPaZr_R-Uol7dVDJfq1WqULJWQvOCMt4MNtQE6_5_ZetfHF2BB0tGkuinCGmOjZQJ9GmRSY97ixS_D-P_Q8zbnNbRdIBoTbZz5OMdlLyTjjjSJ7yuRUSrbu7nUYqV1N1L4mqtVE3dREXTbTu3_ncmf524sG0D1QmhRXNt_f_UjsNaa1yN8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2486884545</pqid></control><display><type>article</type><title>Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls</title><source>SpringerLink Journals</source><creator>Doroteu, Emanuel M. ; Viana, Joao H. M. ; Ferreira Junior, Jair A. ; Macedo, Juliana T. A. ; Oliveira, Rodrigo A. ; Pedroso, Pedro M. O.</creator><creatorcontrib>Doroteu, Emanuel M. ; Viana, Joao H. M. ; Ferreira Junior, Jair A. ; Macedo, Juliana T. A. ; Oliveira, Rodrigo A. ; Pedroso, Pedro M. O.</creatorcontrib><description>The aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (
Bos taurus indicus
) bulls were randomly allocated into three experimental groups: G1 (
n
=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (
n
=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG,
n
=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (
P
=0.0476), width (
P
=0.0021), and in scrotal circumference (
P
=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (
P
<0.01), but in G2, it was also lower than in G1 at day 90 (
P
=0.0006). All sperm parameters were affected by active immunization against GnRH (
P
<0.05), and in G2, averages were lesser (
P
<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.</description><identifier>ISSN: 0049-4747</identifier><identifier>EISSN: 1573-7438</identifier><identifier>DOI: 10.1007/s11250-021-02600-x</identifier><identifier>PMID: 33547980</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; biometry ; blood sampling ; Degeneration ; dose response ; Gonadotropin-releasing hormone ; histopathology ; Immunization ; immunocastration ; Life Sciences ; Nellore ; Regular ; Regular Articles ; Semen ; Seminiferous tubule ; Slaughter ; spermatozoa ; Testes ; Testosterone ; Vaccines ; Veterinary Medicine/Veterinary Science ; zebu ; Zoology</subject><ispartof>Tropical animal health and production, 2021-03, Vol.53 (1), p.153-153, Article 153</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-7fcaefb032a4d3c2c5d59b5816f5ae755f82b03d54982f8915b2b8ea3a2157f13</citedby><cites>FETCH-LOGICAL-c535t-7fcaefb032a4d3c2c5d59b5816f5ae755f82b03d54982f8915b2b8ea3a2157f13</cites><orcidid>0000-0002-7029-6586</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11250-021-02600-x$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11250-021-02600-x$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33547980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doroteu, Emanuel M.</creatorcontrib><creatorcontrib>Viana, Joao H. M.</creatorcontrib><creatorcontrib>Ferreira Junior, Jair A.</creatorcontrib><creatorcontrib>Macedo, Juliana T. A.</creatorcontrib><creatorcontrib>Oliveira, Rodrigo A.</creatorcontrib><creatorcontrib>Pedroso, Pedro M. O.</creatorcontrib><title>Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls</title><title>Tropical animal health and production</title><addtitle>Trop Anim Health Prod</addtitle><addtitle>Trop Anim Health Prod</addtitle><description>The aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (
Bos taurus indicus
) bulls were randomly allocated into three experimental groups: G1 (
n
=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (
n
=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG,
n
=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (
P
=0.0476), width (
P
=0.0021), and in scrotal circumference (
P
=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (
P
<0.01), but in G2, it was also lower than in G1 at day 90 (
P
=0.0006). All sperm parameters were affected by active immunization against GnRH (
P
<0.05), and in G2, averages were lesser (
P
<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.</description><subject>Biomedical and Life Sciences</subject><subject>biometry</subject><subject>blood sampling</subject><subject>Degeneration</subject><subject>dose response</subject><subject>Gonadotropin-releasing hormone</subject><subject>histopathology</subject><subject>Immunization</subject><subject>immunocastration</subject><subject>Life Sciences</subject><subject>Nellore</subject><subject>Regular</subject><subject>Regular Articles</subject><subject>Semen</subject><subject>Seminiferous tubule</subject><subject>Slaughter</subject><subject>spermatozoa</subject><subject>Testes</subject><subject>Testosterone</subject><subject>Vaccines</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>zebu</subject><subject>Zoology</subject><issn>0049-4747</issn><issn>1573-7438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFks9vFCEcxYnR2HX1H_BgSLx4GeXnwFxMTFNbk0aTRs-EYWCXhoUVmNr-92W7tbUe2gTC4X3eI3x5ALzF6CNGSHwqGBOOOkRw2z1C3eUzsMBc0E4wKp-DBUJs6Jhg4gC8KuUcoWaT_UtwQClnYpBoAeYj56ypMDmoYfFxFSxMGdY_CU6p2HIjxLaq747j2Qm80Mb42KAIqy3Vm2bYpLxdp87N0VSfog7QrHXWptrsd0iBPsLvNqRs4TiHUF6DF06HYt_cnkvw6-vRz8OT7vTH8bfDL6ed4ZTXTjijrRsRJZpN1BDDJz6MXOLecW0F506Spk6cDZI4OWA-klFaTTVpY3CYLsHnfe52Hjd2MjbWrIPaZr_R-Uol7dVDJfq1WqULJWQvOCMt4MNtQE6_5_ZetfHF2BB0tGkuinCGmOjZQJ9GmRSY97ixS_D-P_Q8zbnNbRdIBoTbZz5OMdlLyTjjjSJ7yuRUSrbu7nUYqV1N1L4mqtVE3dREXTbTu3_ncmf524sG0D1QmhRXNt_f_UjsNaa1yN8</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Doroteu, Emanuel M.</creator><creator>Viana, Joao H. 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M. ; Ferreira Junior, Jair A. ; Macedo, Juliana T. A. ; Oliveira, Rodrigo A. ; Pedroso, Pedro M. 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M.</au><au>Ferreira Junior, Jair A.</au><au>Macedo, Juliana T. A.</au><au>Oliveira, Rodrigo A.</au><au>Pedroso, Pedro M. O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls</atitle><jtitle>Tropical animal health and production</jtitle><stitle>Trop Anim Health Prod</stitle><addtitle>Trop Anim Health Prod</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>53</volume><issue>1</issue><spage>153</spage><epage>153</epage><pages>153-153</pages><artnum>153</artnum><issn>0049-4747</issn><eissn>1573-7438</eissn><abstract>The aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (
Bos taurus indicus
) bulls were randomly allocated into three experimental groups: G1 (
n
=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (
n
=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG,
n
=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (
P
=0.0476), width (
P
=0.0021), and in scrotal circumference (
P
=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (
P
<0.01), but in G2, it was also lower than in G1 at day 90 (
P
=0.0006). All sperm parameters were affected by active immunization against GnRH (
P
<0.05), and in G2, averages were lesser (
P
<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33547980</pmid><doi>10.1007/s11250-021-02600-x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7029-6586</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-4747 |
| ispartof | Tropical animal health and production, 2021-03, Vol.53 (1), p.153-153, Article 153 |
| issn | 0049-4747 1573-7438 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Biomedical and Life Sciences biometry blood sampling Degeneration dose response Gonadotropin-releasing hormone histopathology Immunization immunocastration Life Sciences Nellore Regular Regular Articles Semen Seminiferous tubule Slaughter spermatozoa Testes Testosterone Vaccines Veterinary Medicine/Veterinary Science zebu Zoology |
| title | Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls |
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