Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA
Radio- and chemoresistance of cancer stem cells (CSCs) is considered as one of the possible causes of adverse results of chemoradiotherapy for various malignancies, including cervical cancer. However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and...
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| Veröffentlicht in: | International journal of molecular sciences 2021-02, Vol.22 (3), p.1445 |
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| creator | Zamulaeva, Irina Selivanova, Elena Matchuk, Olga Kiseleva, Valentina Mkrtchyan, Liana Krikunova, Ludmila |
| description | Radio- and chemoresistance of cancer stem cells (CSCs) is considered as one of the possible causes of adverse results of chemoradiotherapy for various malignancies, including cervical cancer. However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and mechanisms for individual response of CSCs to therapy. The purpose of the study was to evaluate the association of radiation response of cervical CSCs with clinical and morphological parameters of disease and features of human papillomavirus (HPV) infection. The proportion of CD44
CD24
CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86,
= 0.001 for the multiple regression model in the whole). |
| doi_str_mv | 10.3390/ijms22031445 |
| format | Article |
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CD24
CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86,
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CD24
CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86,
= 0.001 for the multiple regression model in the whole).</description><subject>Cancer therapies</subject><subject>CD44 antigen</subject><subject>Cell culture</subject><subject>Cervical cancer</subject><subject>Chemoradiotherapy</subject><subject>Chemoresistance</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Flow cytometry</subject><subject>Genomes</subject><subject>Health services</subject><subject>Human papillomavirus</subject><subject>Infections</subject><subject>Lymph nodes</subject><subject>Lymphatic system</subject><subject>Morphology</subject><subject>Multiple regression models</subject><subject>Patients</subject><subject>Post-radiation</subject><subject>Pretreatment</subject><subject>Radiation dosage</subject><subject>Radiation therapy</subject><subject>Squamous cell carcinoma</subject><subject>Stem cells</subject><subject>Tumors</subject><subject>Uterus</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1PGzEQhi1UBJT2xrmy1AuHBvy1Xu-lUhTagoRoBJSr5diTxtHuOtheKn5C_3VNAijlZHv8zKN5NQgdUXLCeUNO_bJLjBFOhah20AEVjI0IkfW7rfs-ep_SkhDGWdXsoX3OK15Vkh6gv9fGeZN96PE1pFXoE-AwxxOID96aFk9MbyHimwxdKbZtwhcJj1MKtnSBw398XuBphBzB5A76XB5hFeLaWES3CyjGTafpHZ4uHtNafJNNHtITcj69w2dX4w9od27aBB-fz0P06_u328n56PLnj4vJ-HJkBWV5NGNz5hQ4JxQTppGUAREVcO5mSloCgjpXAxA1ayjIhjXcVWBs7ax1zEnLD9HXjXc1zDpwtswcTatX0XcmPupgvP7_p_cL_Ts86FrJmiheBMfPghjuB0hZdz7ZktD0EIakmVBSSEZVXdDPb9BlGGJf4q0ppThltFBfNpSNIaUI89dhKNFPO9bbOy74p-0Ar_DLUvk_QdukZQ</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Zamulaeva, Irina</creator><creator>Selivanova, Elena</creator><creator>Matchuk, Olga</creator><creator>Kiseleva, Valentina</creator><creator>Mkrtchyan, Liana</creator><creator>Krikunova, Ludmila</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6136-8445</orcidid></search><sort><creationdate>20210201</creationdate><title>Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA</title><author>Zamulaeva, Irina ; 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However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and mechanisms for individual response of CSCs to therapy. The purpose of the study was to evaluate the association of radiation response of cervical CSCs with clinical and morphological parameters of disease and features of human papillomavirus (HPV) infection. The proportion of CD44
CD24
CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86,
= 0.001 for the multiple regression model in the whole).</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33535561</pmid><doi>10.3390/ijms22031445</doi><orcidid>https://orcid.org/0000-0002-6136-8445</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Cancer therapies CD44 antigen Cell culture Cervical cancer Chemoradiotherapy Chemoresistance Deoxyribonucleic acid DNA Flow cytometry Genomes Health services Human papillomavirus Infections Lymph nodes Lymphatic system Morphology Multiple regression models Patients Post-radiation Pretreatment Radiation dosage Radiation therapy Squamous cell carcinoma Stem cells Tumors Uterus |
| title | Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA |
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