Detection of SARS-CoV‑2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription Amplification

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global health emergency, and its gene mutation and evolution further posed uncertainty of epidemic risk. Herein, we reported a light-up CRISPR-Cas13 transcription amplification method, which enables the detection o...

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Veröffentlicht in:	Analytical chemistry (Washington) 2021-02, Vol.93 (7), p.3393-3402
Hauptverfasser:	Wang, Yuxi, Zhang, Yong, Chen, Junbo, Wang, Minjin, Zhang, Ting, Luo, Wenxin, Li, Yalun, Wu, Yangping, Zeng, Bo, Zhang, Kaixiang, Deng, Ruijie, Li, Weimin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Aptamers Chemistry Clustered Regularly Interspaced Short Palindromic Repeats - genetics Coronaviridae Coronaviruses COVID-19 - virology COVID-19 Nucleic Acid Testing - methods CRISPR CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics Epidemics Global health Humans Influenza Molecular Diagnostic Techniques Mutation Nucleic Acid Amplification Techniques Point mutation Public health Respiratory diseases Ribonuclease Ribonucleic acid RNA RNA viruses RNA, Viral - genetics SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Transcription Viral diseases Viruses
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