Preexisting and de novo humoral immunity to SARS-CoV-2 in humans

Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2020-12, Vol.370 (6522), p.1339-1343
Hauptverfasser: Ng, Kevin W, Faulkner, Nikhil, Cornish, Georgina H, Rosa, Annachiara, Harvey, Ruth, Hussain, Saira, Ulferts, Rachel, Earl, Christopher, Wrobel, Antoni G, Benton, Donald J, Roustan, Chloe, Bolland, William, Thompson, Rachael, Agua-Doce, Ana, Hobson, Philip, Heaney, Judith, Rickman, Hannah, Paraskevopoulou, Stavroula, Houlihan, Catherine F, Thomson, Kirsty, Sanchez, Emilie, Shin, Gee Yen, Spyer, Moira J, Joshi, Dhira, O'Reilly, Nicola, Walker, Philip A, Kjaer, Svend, Riddell, Andrew, Moore, Catherine, Jebson, Bethany R, Wilkinson, Meredyth, Marshall, Lucy R, Rosser, Elizabeth C, Radziszewska, Anna, Peckham, Hannah, Ciurtin, Coziana, Wedderburn, Lucy R, Beale, Rupert, Swanton, Charles, Gandhi, Sonia, Stockinger, Brigitta, McCauley, John, Gamblin, Steve J, McCoy, Laura E, Cherepanov, Peter, Nastouli, Eleni, Kassiotis, George
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container_end_page 1343
container_issue 6522
container_start_page 1339
container_title Science (American Association for the Advancement of Science)
container_volume 370
creator Ng, Kevin W
Faulkner, Nikhil
Cornish, Georgina H
Rosa, Annachiara
Harvey, Ruth
Hussain, Saira
Ulferts, Rachel
Earl, Christopher
Wrobel, Antoni G
Benton, Donald J
Roustan, Chloe
Bolland, William
Thompson, Rachael
Agua-Doce, Ana
Hobson, Philip
Heaney, Judith
Rickman, Hannah
Paraskevopoulou, Stavroula
Houlihan, Catherine F
Thomson, Kirsty
Sanchez, Emilie
Shin, Gee Yen
Spyer, Moira J
Joshi, Dhira
O'Reilly, Nicola
Walker, Philip A
Kjaer, Svend
Riddell, Andrew
Moore, Catherine
Jebson, Bethany R
Wilkinson, Meredyth
Marshall, Lucy R
Rosser, Elizabeth C
Radziszewska, Anna
Peckham, Hannah
Ciurtin, Coziana
Wedderburn, Lucy R
Beale, Rupert
Swanton, Charles
Gandhi, Sonia
Stockinger, Brigitta
McCauley, John
Gamblin, Steve J
McCoy, Laura E
Cherepanov, Peter
Nastouli, Eleni
Kassiotis, George
description Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.
doi_str_mv 10.1126/science.abe1107
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Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. 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Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.</description><subject>Adolescents</subject><subject>Adult</subject><subject>Age composition</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Children</subject><subject>Coronaviridae</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 - blood</subject><subject>COVID-19 - immunology</subject><subject>Cross-protection</subject><subject>Epitope Mapping</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Glycoproteins</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Humoral immunity</subject><subject>IgG antibody</subject><subject>Immunity</subject><subject>Immunity, 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and de novo humoral immunity to SARS-CoV-2 in humans</title><author>Ng, Kevin W ; Faulkner, Nikhil ; Cornish, Georgina H ; Rosa, Annachiara ; Harvey, Ruth ; Hussain, Saira ; Ulferts, Rachel ; Earl, Christopher ; Wrobel, Antoni G ; Benton, Donald J ; Roustan, Chloe ; Bolland, William ; Thompson, Rachael ; Agua-Doce, Ana ; Hobson, Philip ; Heaney, Judith ; Rickman, Hannah ; Paraskevopoulou, Stavroula ; Houlihan, Catherine F ; Thomson, Kirsty ; Sanchez, Emilie ; Shin, Gee Yen ; Spyer, Moira J ; Joshi, Dhira ; O'Reilly, Nicola ; Walker, Philip A ; Kjaer, Svend ; Riddell, Andrew ; Moore, Catherine ; Jebson, Bethany R ; Wilkinson, Meredyth ; Marshall, Lucy R ; Rosser, Elizabeth C ; Radziszewska, Anna ; Peckham, Hannah ; Ciurtin, Coziana ; Wedderburn, Lucy R ; Beale, Rupert ; Swanton, Charles ; Gandhi, Sonia ; Stockinger, Brigitta ; McCauley, John ; Gamblin, Steve J ; McCoy, Laura E ; Cherepanov, Peter ; Nastouli, Eleni ; Kassiotis, George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-94e8c832a40a1ad6f18e5be1135a8a1b48bfdeed8aa69051b179c5cf8b9130043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescents</topic><topic>Adult</topic><topic>Age composition</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Children</topic><topic>Coronaviridae</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>COVID-19 - blood</topic><topic>COVID-19 - immunology</topic><topic>Cross-protection</topic><topic>Epitope Mapping</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Glycoproteins</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Humoral immunity</topic><topic>IgG antibody</topic><topic>Immunity</topic><topic>Immunity, Humoral</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin A - blood</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M</topic><topic>Immunoglobulin M - blood</topic><topic>Immunoglobulins</topic><topic>Immunological memory</topic><topic>Immunology</topic><topic>Infections</topic><topic>Male</topic><topic>Microbio</topic><topic>Middle Aged</topic><topic>Neutralizing</topic><topic>Proteins</topic><topic>Respiratory diseases</topic><topic>SARS-CoV-2 - chemistry</topic><topic>SARS-CoV-2 - immunology</topic><topic>Severe acute respiratory syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Spike glycoprotein</topic><topic>Spike Glycoprotein, Coronavirus - chemistry</topic><topic>Spike Glycoprotein, Coronavirus - immunology</topic><topic>Spike protein</topic><topic>Teenagers</topic><topic>Viral diseases</topic><topic>Viral Zoonoses - blood</topic><topic>Viral Zoonoses - immunology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, Kevin W</creatorcontrib><creatorcontrib>Faulkner, Nikhil</creatorcontrib><creatorcontrib>Cornish, Georgina H</creatorcontrib><creatorcontrib>Rosa, Annachiara</creatorcontrib><creatorcontrib>Harvey, Ruth</creatorcontrib><creatorcontrib>Hussain, Saira</creatorcontrib><creatorcontrib>Ulferts, Rachel</creatorcontrib><creatorcontrib>Earl, Christopher</creatorcontrib><creatorcontrib>Wrobel, Antoni G</creatorcontrib><creatorcontrib>Benton, Donald J</creatorcontrib><creatorcontrib>Roustan, Chloe</creatorcontrib><creatorcontrib>Bolland, William</creatorcontrib><creatorcontrib>Thompson, Rachael</creatorcontrib><creatorcontrib>Agua-Doce, Ana</creatorcontrib><creatorcontrib>Hobson, Philip</creatorcontrib><creatorcontrib>Heaney, Judith</creatorcontrib><creatorcontrib>Rickman, Hannah</creatorcontrib><creatorcontrib>Paraskevopoulou, Stavroula</creatorcontrib><creatorcontrib>Houlihan, Catherine F</creatorcontrib><creatorcontrib>Thomson, Kirsty</creatorcontrib><creatorcontrib>Sanchez, Emilie</creatorcontrib><creatorcontrib>Shin, Gee Yen</creatorcontrib><creatorcontrib>Spyer, Moira J</creatorcontrib><creatorcontrib>Joshi, Dhira</creatorcontrib><creatorcontrib>O'Reilly, Nicola</creatorcontrib><creatorcontrib>Walker, Philip A</creatorcontrib><creatorcontrib>Kjaer, Svend</creatorcontrib><creatorcontrib>Riddell, Andrew</creatorcontrib><creatorcontrib>Moore, Catherine</creatorcontrib><creatorcontrib>Jebson, Bethany R</creatorcontrib><creatorcontrib>Wilkinson, Meredyth</creatorcontrib><creatorcontrib>Marshall, Lucy R</creatorcontrib><creatorcontrib>Rosser, Elizabeth C</creatorcontrib><creatorcontrib>Radziszewska, Anna</creatorcontrib><creatorcontrib>Peckham, Hannah</creatorcontrib><creatorcontrib>Ciurtin, Coziana</creatorcontrib><creatorcontrib>Wedderburn, Lucy R</creatorcontrib><creatorcontrib>Beale, Rupert</creatorcontrib><creatorcontrib>Swanton, Charles</creatorcontrib><creatorcontrib>Gandhi, Sonia</creatorcontrib><creatorcontrib>Stockinger, Brigitta</creatorcontrib><creatorcontrib>McCauley, John</creatorcontrib><creatorcontrib>Gamblin, Steve J</creatorcontrib><creatorcontrib>McCoy, Laura E</creatorcontrib><creatorcontrib>Cherepanov, Peter</creatorcontrib><creatorcontrib>Nastouli, Eleni</creatorcontrib><creatorcontrib>Kassiotis, George</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and 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for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Kevin W</au><au>Faulkner, Nikhil</au><au>Cornish, Georgina H</au><au>Rosa, Annachiara</au><au>Harvey, Ruth</au><au>Hussain, Saira</au><au>Ulferts, Rachel</au><au>Earl, Christopher</au><au>Wrobel, Antoni G</au><au>Benton, Donald J</au><au>Roustan, Chloe</au><au>Bolland, William</au><au>Thompson, Rachael</au><au>Agua-Doce, Ana</au><au>Hobson, Philip</au><au>Heaney, Judith</au><au>Rickman, Hannah</au><au>Paraskevopoulou, Stavroula</au><au>Houlihan, Catherine F</au><au>Thomson, Kirsty</au><au>Sanchez, Emilie</au><au>Shin, Gee Yen</au><au>Spyer, Moira J</au><au>Joshi, Dhira</au><au>O'Reilly, Nicola</au><au>Walker, Philip A</au><au>Kjaer, Svend</au><au>Riddell, Andrew</au><au>Moore, Catherine</au><au>Jebson, Bethany R</au><au>Wilkinson, Meredyth</au><au>Marshall, Lucy R</au><au>Rosser, Elizabeth C</au><au>Radziszewska, Anna</au><au>Peckham, Hannah</au><au>Ciurtin, Coziana</au><au>Wedderburn, Lucy R</au><au>Beale, Rupert</au><au>Swanton, Charles</au><au>Gandhi, Sonia</au><au>Stockinger, Brigitta</au><au>McCauley, John</au><au>Gamblin, Steve J</au><au>McCoy, Laura E</au><au>Cherepanov, Peter</au><au>Nastouli, Eleni</au><au>Kassiotis, George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preexisting and de novo humoral immunity to SARS-CoV-2 in humans</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2020-12-11</date><risdate>2020</risdate><volume>370</volume><issue>6522</issue><spage>1339</spage><epage>1343</epage><pages>1339-1343</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>33159009</pmid><doi>10.1126/science.abe1107</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1984-6677</orcidid><orcidid>https://orcid.org/0000-0001-9767-8683</orcidid><orcidid>https://orcid.org/0000-0001-8781-336X</orcidid><orcidid>https://orcid.org/0000-0001-9503-7946</orcidid><orcidid>https://orcid.org/0000-0001-6748-9339</orcidid><orcidid>https://orcid.org/0000-0002-9091-9955</orcidid><orcidid>https://orcid.org/0000-0002-8911-4113</orcidid><orcidid>https://orcid.org/0000-0002-9668-4683</orcidid><orcidid>https://orcid.org/0000-0002-7539-1566</orcidid><orcidid>https://orcid.org/0000-0002-3625-776X</orcidid><orcidid>https://orcid.org/0000-0002-1684-2013</orcidid><orcidid>https://orcid.org/0000-0002-6705-8560</orcidid><orcidid>https://orcid.org/0000-0002-2794-8606</orcidid><orcidid>https://orcid.org/0000-0002-8457-2633</orcidid><orcidid>https://orcid.org/0000-0001-8660-2528</orcidid><orcidid>https://orcid.org/0000-0002-4204-6491</orcidid><orcidid>https://orcid.org/0000-0001-5117-2994</orcidid><orcidid>https://orcid.org/0000-0002-3502-0744</orcidid><orcidid>https://orcid.org/0000-0002-3564-1970</orcidid><orcidid>https://orcid.org/0000-0002-4062-530X</orcidid><orcidid>https://orcid.org/0000-0002-4299-3018</orcidid><orcidid>https://orcid.org/0000-0001-8934-0930</orcidid><orcidid>https://orcid.org/0000-0003-1635-6768</orcidid><orcidid>https://orcid.org/0000-0002-6680-5587</orcidid><orcidid>https://orcid.org/0000-0002-7972-8066</orcidid><orcidid>https://orcid.org/0000-0001-9149-6029</orcidid><orcidid>https://orcid.org/0000-0002-3885-3477</orcidid><orcidid>https://orcid.org/0000-0002-0545-244X</orcidid><orcidid>https://orcid.org/0000-0003-4800-4695</orcidid><orcidid>https://orcid.org/0000-0001-5331-639X</orcidid><orcidid>https://orcid.org/0000-0002-0634-538X</orcidid><orcidid>https://orcid.org/0000-0002-7148-4634</orcidid><orcidid>https://orcid.org/0000-0001-7433-6357</orcidid><orcidid>https://orcid.org/0000-0002-4744-6347</orcidid><orcidid>https://orcid.org/0000-0002-8439-8997</orcidid><orcidid>https://orcid.org/0000-0002-0466-7297</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0036-8075
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issn 0036-8075
1095-9203
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7857411
source MEDLINE; American Association for the Advancement of Science
subjects Adolescents
Adult
Age composition
Aged
Aged, 80 and over
Amino Acid Sequence
Animals
Antibodies
Antibodies, Viral - blood
Children
Coronaviridae
Coronaviruses
COVID-19
COVID-19 - blood
COVID-19 - immunology
Cross-protection
Epitope Mapping
Female
Flow cytometry
Glycoproteins
HEK293 Cells
Humans
Humoral immunity
IgG antibody
Immunity
Immunity, Humoral
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M
Immunoglobulin M - blood
Immunoglobulins
Immunological memory
Immunology
Infections
Male
Microbio
Middle Aged
Neutralizing
Proteins
Respiratory diseases
SARS-CoV-2 - chemistry
SARS-CoV-2 - immunology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Spike glycoprotein
Spike Glycoprotein, Coronavirus - chemistry
Spike Glycoprotein, Coronavirus - immunology
Spike protein
Teenagers
Viral diseases
Viral Zoonoses - blood
Viral Zoonoses - immunology
Young Adult
title Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
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