Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation

We have studied the role of protein dynamics in chemical catalysis in the enzyme dihydrofolate reductase (DHFR), using a pump–probe method that employs pulsed-laser photothermal heating of a gold nanoparticle (AuNP) to directly excite a local region of the protein structure and transient absorbance...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2021-01, Vol.118 (4), p.1-8
Hauptverfasser:	Kozlowski, Rachel, Zhao, Jing, Dyer, R. Brian
Format:	Artikel
Sprache:	eng
Schlagworte:	Physical Sciences
Online-Zugang:	Volltext
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