Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels
Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibi...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2021-01, Vol.118 (4), p.1-7 |
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| creator | Batalov, Ivan Stevens, Kelly R. DeForest, Cole A. |
| description | Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibility, matrix remodeling, and biological integration. Despite these advantages, natural protein-based gels have lagged behind synthetic alternatives in their tunability; methods to selectively modulate the biochemical properties of these networks in a user-defined and heterogeneous fashion that can drive encapsulated cell function have not yet been established. Here, we report a generalizable strategy utilizing a photomediated oxime ligation to covalently decorate naturally derived hydrogels with bioactive proteins including growth factors. This bioorthogonal photofunctionalization is readily amenable to mask-based and laser-scanning lithographic patterning, enabling full four-dimensional (4D) control over protein immobilization within virtually any natural protein-based biomaterial. Such versatility affords exciting opportunities to probe and direct advanced cell fates inaccessible using purely synthetic approaches in response to anisotropic environmental signaling. |
| doi_str_mv | 10.1073/pnas.2014194118 |
| format | Article |
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In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibility, matrix remodeling, and biological integration. Despite these advantages, natural protein-based gels have lagged behind synthetic alternatives in their tunability; methods to selectively modulate the biochemical properties of these networks in a user-defined and heterogeneous fashion that can drive encapsulated cell function have not yet been established. Here, we report a generalizable strategy utilizing a photomediated oxime ligation to covalently decorate naturally derived hydrogels with bioactive proteins including growth factors. This bioorthogonal photofunctionalization is readily amenable to mask-based and laser-scanning lithographic patterning, enabling full four-dimensional (4D) control over protein immobilization within virtually any natural protein-based biomaterial. 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| subjects | Biological Products - chemistry Biological Products - pharmacology Biological Sciences Cell Culture Techniques - methods Cell Differentiation - drug effects Cell Line Cell Lineage Humans Hydrogels - chemistry Hydrogels - pharmacology Intercellular Signaling Peptides and Proteins - chemistry Intercellular Signaling Peptides and Proteins - pharmacology Polymers - chemistry Proteins - chemistry Tissue Engineering - methods |
| title | Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels |
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