Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates
Background: This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates. Methods: A retrospective analysis was performed on CRKP strains isolated from a teachi...
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| Veröffentlicht in: | Infection and drug resistance 2021-01, Vol.14, p.237-247 |
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| creator | Liu, Hongmao Lin, Hailong Sun, Zhewei Zhu, Xinyi Zhang, Xueya Li, Qiaoling Lu, Junwan Lin, Xi Lin, Li Li, Kewei Zhu, Mei Bao, Qiyu Xu, Teng Hu, Yunliang Zhang, Hailin |
| description | Background: This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.
Methods: A retrospective analysis was performed on CRKP strains isolated from a teaching hospital of Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification and whole-genome sequencing (WGS) were used to analyze the genetic context of the bla(KPC-2) gene. Conjugation experiments were performed to evaluate the transferability of bla(KPC-2)-bearing plasmids. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to investigate the clonal relatedness of bla(KPC-2)-producing strains.
Results: The bla(KPC-2) gene was identified from 13.61% (40/294) of clinical K. pneumoniae isolates. Three different sequence types (ST11, ST15 and ST656) and 5 PFGE subtypes (A to E) were classified among them. ST11 was the dominant sequence type (92.50%, 37/40). Plasmid-oriented antibiotic resistance genes, such as extended spectrum-beta-lactamases (ESBL5) and other antimicrobial resistance genes, were also found in KPC-positive K. pneumoniae (KPC-Kp) isolates. Mapping PCR and genomic sequencing revealed that the bla(KPC-2)-bearing sequence regions, which are related to different mobile elements, including Tn1721- and IS26-based transposons, were mainly located in but not restricted to IncFII-like plasmids and were structurally divergent.
Conclusion: The bla(KPC-2) genes related to divergent mobile genetic elements encoded on transferable plasmids may transfer widely, facilitating the spread of carbapenem resistance among bacteria with different genetic backgrounds. The dissemination of bla(KPC)-bearing plasmids that collectively carry additional multidrug resistance genes has caused widespread public concern, further limiting the antibiotics available to treat infections caused by KPC-producing pathogens. |
| doi_str_mv | 10.2147/IDR.S290434 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7847768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_979a4d52ff464d0d810b067355397ffd</doaj_id><sourcerecordid>2484316016</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-a6617465d7fffa245751767004161d2c34b52bc9389fdd3078cea64eb72c54903</originalsourceid><addsrcrecordid>eNqNkt1uFCEUxydGY5vaK1-AxEszFQYGhhsTM9W66SY2flyTM8CsbGZhBcaPB_CFfBCfSaa7qfZObiDwO7-TQ_5V9ZTgi4Yw8WJ1-f7iQyMxo-xBdUqI6GouBX34z_mkOk9pi8uikjPRPK5OKG0pF5yfVj8vXcrRDXN2waMwot-_6jXoDDtIFl1ZbxMCb25P2WnUB5_t97yQwwTXN33dIOdRPznvNEyohzjAvsBLfX0Tg5m18xt0PdkhOTtNgPbezrvgHVi0SmGCbNOT6tEIU7Lnx_2s-vTm9cf-bb1-d7XqX61rzYjMNXBOBOOtEeM4QsNa0RLBBcaMcGIaTdnQNoOWtJOjMRSLTlvgzA6i0S2TmJ5Vq4PXBNiqfXQ7iD9UAKduL0LcKIhlzMkqKSQw0zbjyDgz2HQED5gL2rZUlvamuF4eXPt52Fmjrc8RpnvS-y_efVab8FWJjgnBuyJ4dhTE8GW2KattmKMv86uGdYwSjgkv1PMDpWNIKdrxrgPBaomAKhFQxwj8pb_ZIYxJO-u1vasoESgfyJlkSxpIobv_p3uXYQlJH2af6R-KD8PJ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2484316016</pqid></control><display><type>article</type><title>Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates</title><source>Taylor & Francis Open Access</source><source>DOVE Medical Press Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><source>PubMed Central</source><creator>Liu, Hongmao ; Lin, Hailong ; Sun, Zhewei ; Zhu, Xinyi ; Zhang, Xueya ; Li, Qiaoling ; Lu, Junwan ; Lin, Xi ; Lin, Li ; Li, Kewei ; Zhu, Mei ; Bao, Qiyu ; Xu, Teng ; Hu, Yunliang ; Zhang, Hailin</creator><creatorcontrib>Liu, Hongmao ; Lin, Hailong ; Sun, Zhewei ; Zhu, Xinyi ; Zhang, Xueya ; Li, Qiaoling ; Lu, Junwan ; Lin, Xi ; Lin, Li ; Li, Kewei ; Zhu, Mei ; Bao, Qiyu ; Xu, Teng ; Hu, Yunliang ; Zhang, Hailin</creatorcontrib><description>Background: This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.
Methods: A retrospective analysis was performed on CRKP strains isolated from a teaching hospital of Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification and whole-genome sequencing (WGS) were used to analyze the genetic context of the bla(KPC-2) gene. Conjugation experiments were performed to evaluate the transferability of bla(KPC-2)-bearing plasmids. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to investigate the clonal relatedness of bla(KPC-2)-producing strains.
Results: The bla(KPC-2) gene was identified from 13.61% (40/294) of clinical K. pneumoniae isolates. Three different sequence types (ST11, ST15 and ST656) and 5 PFGE subtypes (A to E) were classified among them. ST11 was the dominant sequence type (92.50%, 37/40). Plasmid-oriented antibiotic resistance genes, such as extended spectrum-beta-lactamases (ESBL5) and other antimicrobial resistance genes, were also found in KPC-positive K. pneumoniae (KPC-Kp) isolates. Mapping PCR and genomic sequencing revealed that the bla(KPC-2)-bearing sequence regions, which are related to different mobile elements, including Tn1721- and IS26-based transposons, were mainly located in but not restricted to IncFII-like plasmids and were structurally divergent.
Conclusion: The bla(KPC-2) genes related to divergent mobile genetic elements encoded on transferable plasmids may transfer widely, facilitating the spread of carbapenem resistance among bacteria with different genetic backgrounds. The dissemination of bla(KPC)-bearing plasmids that collectively carry additional multidrug resistance genes has caused widespread public concern, further limiting the antibiotics available to treat infections caused by KPC-producing pathogens.</description><identifier>ISSN: 1178-6973</identifier><identifier>EISSN: 1178-6973</identifier><identifier>DOI: 10.2147/IDR.S290434</identifier><identifier>PMID: 33536766</identifier><language>eng</language><publisher>ALBANY: Dove Medical Press Ltd</publisher><subject>Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Antimicrobial resistance ; Bacterial infections ; blakpc-2 ; Carbapenemase ; Chromosomes ; Cloning ; Conjugation ; crkp ; Deoxyribonucleic acid ; DNA ; Drug resistance ; Gel electrophoresis ; Gene mapping ; Genes ; Genomes ; Genomics ; Infectious Diseases ; Klebsiella pneumoniae ; kpc-kp ; Life Sciences & Biomedicine ; Multidrug resistance ; Multilocus sequence typing ; Original Research ; Pharmacology & Pharmacy ; Plasmids ; Polymerase chain reaction ; Proteins ; Pulsed-field gel electrophoresis ; Science & Technology ; st11 ; Strains (organisms) ; tn1721 ; Transposons ; Whole genome sequencing ; β Lactamase</subject><ispartof>Infection and drug resistance, 2021-01, Vol.14, p.237-247</ispartof><rights>2021. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Liu et al. 2021 Liu et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>12</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000617649400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c419t-a6617465d7fffa245751767004161d2c34b52bc9389fdd3078cea64eb72c54903</citedby><cites>FETCH-LOGICAL-c419t-a6617465d7fffa245751767004161d2c34b52bc9389fdd3078cea64eb72c54903</cites><orcidid>0000-0002-1421-5593 ; 0000-0002-8136-2235</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847768/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847768/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,3863,27929,27930,39263,53796,53798</link.rule.ids></links><search><creatorcontrib>Liu, Hongmao</creatorcontrib><creatorcontrib>Lin, Hailong</creatorcontrib><creatorcontrib>Sun, Zhewei</creatorcontrib><creatorcontrib>Zhu, Xinyi</creatorcontrib><creatorcontrib>Zhang, Xueya</creatorcontrib><creatorcontrib>Li, Qiaoling</creatorcontrib><creatorcontrib>Lu, Junwan</creatorcontrib><creatorcontrib>Lin, Xi</creatorcontrib><creatorcontrib>Lin, Li</creatorcontrib><creatorcontrib>Li, Kewei</creatorcontrib><creatorcontrib>Zhu, Mei</creatorcontrib><creatorcontrib>Bao, Qiyu</creatorcontrib><creatorcontrib>Xu, Teng</creatorcontrib><creatorcontrib>Hu, Yunliang</creatorcontrib><creatorcontrib>Zhang, Hailin</creatorcontrib><title>Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates</title><title>Infection and drug resistance</title><addtitle>INFECT DRUG RESIST</addtitle><description>Background: This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.
Methods: A retrospective analysis was performed on CRKP strains isolated from a teaching hospital of Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification and whole-genome sequencing (WGS) were used to analyze the genetic context of the bla(KPC-2) gene. Conjugation experiments were performed to evaluate the transferability of bla(KPC-2)-bearing plasmids. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to investigate the clonal relatedness of bla(KPC-2)-producing strains.
Results: The bla(KPC-2) gene was identified from 13.61% (40/294) of clinical K. pneumoniae isolates. Three different sequence types (ST11, ST15 and ST656) and 5 PFGE subtypes (A to E) were classified among them. ST11 was the dominant sequence type (92.50%, 37/40). Plasmid-oriented antibiotic resistance genes, such as extended spectrum-beta-lactamases (ESBL5) and other antimicrobial resistance genes, were also found in KPC-positive K. pneumoniae (KPC-Kp) isolates. Mapping PCR and genomic sequencing revealed that the bla(KPC-2)-bearing sequence regions, which are related to different mobile elements, including Tn1721- and IS26-based transposons, were mainly located in but not restricted to IncFII-like plasmids and were structurally divergent.
Conclusion: The bla(KPC-2) genes related to divergent mobile genetic elements encoded on transferable plasmids may transfer widely, facilitating the spread of carbapenem resistance among bacteria with different genetic backgrounds. The dissemination of bla(KPC)-bearing plasmids that collectively carry additional multidrug resistance genes has caused widespread public concern, further limiting the antibiotics available to treat infections caused by KPC-producing pathogens.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacterial infections</subject><subject>blakpc-2</subject><subject>Carbapenemase</subject><subject>Chromosomes</subject><subject>Cloning</subject><subject>Conjugation</subject><subject>crkp</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Gel electrophoresis</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Infectious Diseases</subject><subject>Klebsiella pneumoniae</subject><subject>kpc-kp</subject><subject>Life Sciences & Biomedicine</subject><subject>Multidrug resistance</subject><subject>Multilocus sequence typing</subject><subject>Original Research</subject><subject>Pharmacology & Pharmacy</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Pulsed-field gel electrophoresis</subject><subject>Science & Technology</subject><subject>st11</subject><subject>Strains (organisms)</subject><subject>tn1721</subject><subject>Transposons</subject><subject>Whole genome sequencing</subject><subject>β Lactamase</subject><issn>1178-6973</issn><issn>1178-6973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNqNkt1uFCEUxydGY5vaK1-AxEszFQYGhhsTM9W66SY2flyTM8CsbGZhBcaPB_CFfBCfSaa7qfZObiDwO7-TQ_5V9ZTgi4Yw8WJ1-f7iQyMxo-xBdUqI6GouBX34z_mkOk9pi8uikjPRPK5OKG0pF5yfVj8vXcrRDXN2waMwot-_6jXoDDtIFl1ZbxMCb25P2WnUB5_t97yQwwTXN33dIOdRPznvNEyohzjAvsBLfX0Tg5m18xt0PdkhOTtNgPbezrvgHVi0SmGCbNOT6tEIU7Lnx_2s-vTm9cf-bb1-d7XqX61rzYjMNXBOBOOtEeM4QsNa0RLBBcaMcGIaTdnQNoOWtJOjMRSLTlvgzA6i0S2TmJ5Vq4PXBNiqfXQ7iD9UAKduL0LcKIhlzMkqKSQw0zbjyDgz2HQED5gL2rZUlvamuF4eXPt52Fmjrc8RpnvS-y_efVab8FWJjgnBuyJ4dhTE8GW2KattmKMv86uGdYwSjgkv1PMDpWNIKdrxrgPBaomAKhFQxwj8pb_ZIYxJO-u1vasoESgfyJlkSxpIobv_p3uXYQlJH2af6R-KD8PJ</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Liu, Hongmao</creator><creator>Lin, Hailong</creator><creator>Sun, Zhewei</creator><creator>Zhu, Xinyi</creator><creator>Zhang, Xueya</creator><creator>Li, Qiaoling</creator><creator>Lu, Junwan</creator><creator>Lin, Xi</creator><creator>Lin, Li</creator><creator>Li, Kewei</creator><creator>Zhu, Mei</creator><creator>Bao, Qiyu</creator><creator>Xu, Teng</creator><creator>Hu, Yunliang</creator><creator>Zhang, Hailin</creator><general>Dove Medical Press Ltd</general><general>Taylor & Francis Ltd</general><general>Dove</general><general>Dove Medical Press</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1421-5593</orcidid><orcidid>https://orcid.org/0000-0002-8136-2235</orcidid></search><sort><creationdate>20210101</creationdate><title>Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates</title><author>Liu, Hongmao ; Lin, Hailong ; Sun, Zhewei ; Zhu, Xinyi ; Zhang, Xueya ; Li, Qiaoling ; Lu, Junwan ; Lin, Xi ; Lin, Li ; Li, Kewei ; Zhu, Mei ; Bao, Qiyu ; Xu, Teng ; Hu, Yunliang ; Zhang, Hailin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-a6617465d7fffa245751767004161d2c34b52bc9389fdd3078cea64eb72c54903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Bacterial infections</topic><topic>blakpc-2</topic><topic>Carbapenemase</topic><topic>Chromosomes</topic><topic>Cloning</topic><topic>Conjugation</topic><topic>crkp</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Gel electrophoresis</topic><topic>Gene mapping</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Infectious Diseases</topic><topic>Klebsiella pneumoniae</topic><topic>kpc-kp</topic><topic>Life Sciences & Biomedicine</topic><topic>Multidrug resistance</topic><topic>Multilocus sequence typing</topic><topic>Original Research</topic><topic>Pharmacology & Pharmacy</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Pulsed-field gel electrophoresis</topic><topic>Science & Technology</topic><topic>st11</topic><topic>Strains (organisms)</topic><topic>tn1721</topic><topic>Transposons</topic><topic>Whole genome sequencing</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hongmao</creatorcontrib><creatorcontrib>Lin, Hailong</creatorcontrib><creatorcontrib>Sun, Zhewei</creatorcontrib><creatorcontrib>Zhu, Xinyi</creatorcontrib><creatorcontrib>Zhang, Xueya</creatorcontrib><creatorcontrib>Li, Qiaoling</creatorcontrib><creatorcontrib>Lu, Junwan</creatorcontrib><creatorcontrib>Lin, Xi</creatorcontrib><creatorcontrib>Lin, Li</creatorcontrib><creatorcontrib>Li, Kewei</creatorcontrib><creatorcontrib>Zhu, Mei</creatorcontrib><creatorcontrib>Bao, Qiyu</creatorcontrib><creatorcontrib>Xu, Teng</creatorcontrib><creatorcontrib>Hu, Yunliang</creatorcontrib><creatorcontrib>Zhang, Hailin</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Infection and drug resistance</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hongmao</au><au>Lin, Hailong</au><au>Sun, Zhewei</au><au>Zhu, Xinyi</au><au>Zhang, Xueya</au><au>Li, Qiaoling</au><au>Lu, Junwan</au><au>Lin, Xi</au><au>Lin, Li</au><au>Li, Kewei</au><au>Zhu, Mei</au><au>Bao, Qiyu</au><au>Xu, Teng</au><au>Hu, Yunliang</au><au>Zhang, Hailin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates</atitle><jtitle>Infection and drug resistance</jtitle><stitle>INFECT DRUG RESIST</stitle><date>2021-01-01</date><risdate>2021</risdate><volume>14</volume><spage>237</spage><epage>247</epage><pages>237-247</pages><issn>1178-6973</issn><eissn>1178-6973</eissn><abstract>Background: This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.
Methods: A retrospective analysis was performed on CRKP strains isolated from a teaching hospital of Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification and whole-genome sequencing (WGS) were used to analyze the genetic context of the bla(KPC-2) gene. Conjugation experiments were performed to evaluate the transferability of bla(KPC-2)-bearing plasmids. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed to investigate the clonal relatedness of bla(KPC-2)-producing strains.
Results: The bla(KPC-2) gene was identified from 13.61% (40/294) of clinical K. pneumoniae isolates. Three different sequence types (ST11, ST15 and ST656) and 5 PFGE subtypes (A to E) were classified among them. ST11 was the dominant sequence type (92.50%, 37/40). Plasmid-oriented antibiotic resistance genes, such as extended spectrum-beta-lactamases (ESBL5) and other antimicrobial resistance genes, were also found in KPC-positive K. pneumoniae (KPC-Kp) isolates. Mapping PCR and genomic sequencing revealed that the bla(KPC-2)-bearing sequence regions, which are related to different mobile elements, including Tn1721- and IS26-based transposons, were mainly located in but not restricted to IncFII-like plasmids and were structurally divergent.
Conclusion: The bla(KPC-2) genes related to divergent mobile genetic elements encoded on transferable plasmids may transfer widely, facilitating the spread of carbapenem resistance among bacteria with different genetic backgrounds. The dissemination of bla(KPC)-bearing plasmids that collectively carry additional multidrug resistance genes has caused widespread public concern, further limiting the antibiotics available to treat infections caused by KPC-producing pathogens.</abstract><cop>ALBANY</cop><pub>Dove Medical Press Ltd</pub><pmid>33536766</pmid><doi>10.2147/IDR.S290434</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1421-5593</orcidid><orcidid>https://orcid.org/0000-0002-8136-2235</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Infection and drug resistance, 2021-01, Vol.14, p.237-247 |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; PubMed Central |
| subjects | Antibiotic resistance Antibiotics Antimicrobial agents Antimicrobial resistance Bacterial infections blakpc-2 Carbapenemase Chromosomes Cloning Conjugation crkp Deoxyribonucleic acid DNA Drug resistance Gel electrophoresis Gene mapping Genes Genomes Genomics Infectious Diseases Klebsiella pneumoniae kpc-kp Life Sciences & Biomedicine Multidrug resistance Multilocus sequence typing Original Research Pharmacology & Pharmacy Plasmids Polymerase chain reaction Proteins Pulsed-field gel electrophoresis Science & Technology st11 Strains (organisms) tn1721 Transposons Whole genome sequencing β Lactamase |
| title | Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T11%3A35%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution%20of%20%CE%B2-Lactamase%20Genes%20and%20Genetic%20Context%20of%20blaKPC-2%20in%20Clinical%20Carbapenemase-Producing%20Klebsiella%20pneumoniae%20Isolates&rft.jtitle=Infection%20and%20drug%20resistance&rft.au=Liu,%20Hongmao&rft.date=2021-01-01&rft.volume=14&rft.spage=237&rft.epage=247&rft.pages=237-247&rft.issn=1178-6973&rft.eissn=1178-6973&rft_id=info:doi/10.2147/IDR.S290434&rft_dat=%3Cproquest_pubme%3E2484316016%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2484316016&rft_id=info:pmid/33536766&rft_doaj_id=oai_doaj_org_article_979a4d52ff464d0d810b067355397ffd&rfr_iscdi=true |