Large pH oscillations promote host defense against human airways infection
The airway mucosal microenvironment is crucial for host defense against inhaled pathogens but remains poorly understood. We report here that the airway surface normally undergoes surprisingly large excursions in pH during breathing that can reach pH 9.0 during inhalation, making it the most alkaline...
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| Veröffentlicht in: | The Journal of experimental medicine 2021-04, Vol.218 (4) |
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| container_title | The Journal of experimental medicine |
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| creator | Kim, Dusik Liao, Jie Scales, Nathan B Martini, Carolina Luan, Xiaojie Abu-Arish, Asmahan Robert, Renaud Luo, Yishan McKay, Geoffrey A Nguyen, Dao Tewfik, Marc A Poirier, Charles D Matouk, Elias Ianowski, Juan P Frenkiel, Saul Hanrahan, John W |
| description | The airway mucosal microenvironment is crucial for host defense against inhaled pathogens but remains poorly understood. We report here that the airway surface normally undergoes surprisingly large excursions in pH during breathing that can reach pH 9.0 during inhalation, making it the most alkaline fluid in the body. Transient alkalinization requires luminal bicarbonate and membrane-bound carbonic anhydrase 12 (CA12) and is antimicrobial. Luminal bicarbonate concentration and CA12 expression are both reduced in cystic fibrosis (CF), and mucus accumulation both buffers the pH and obstructs airflow, further suppressing the oscillations and bacterial-killing efficacy. Defective pH oscillations may compromise airway host defense in other respiratory diseases and explain CF-like airway infections in people with CA12 mutations. |
| doi_str_mv | 10.1084/jem.20201831 |
| format | Article |
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We report here that the airway surface normally undergoes surprisingly large excursions in pH during breathing that can reach pH 9.0 during inhalation, making it the most alkaline fluid in the body. Transient alkalinization requires luminal bicarbonate and membrane-bound carbonic anhydrase 12 (CA12) and is antimicrobial. Luminal bicarbonate concentration and CA12 expression are both reduced in cystic fibrosis (CF), and mucus accumulation both buffers the pH and obstructs airflow, further suppressing the oscillations and bacterial-killing efficacy. 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| title | Large pH oscillations promote host defense against human airways infection |
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