Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection
Background The GABA‐A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. Methods To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2021-01, Vol.35 (1), p.e23584-n/a |
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| creator | Zhang, Zili Wang, Ting Huang, Juanhua Huang, Yonghan Zhang, Qingxue |
| description | Background
The GABA‐A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development.
Methods
To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression dataset, GSE23009, was obtained from the Gene Expression Omnibus database. Genes with differential expression were identified using the R software package, and RT‐qPCR was performed to confirm the microarray results.
Results
Mouse blastocysts derived from ICSI fertilization had decreased expression of GABA‐A receptor signaling pathway genes. However, the mechanisms underlying these changes were not elucidated. The gene expression of the GABA‐A pathway was not significantly different between blastocysts obtained from IVF and in vivo fertilization. However, microinjection after IVF significantly reduced the expression of the GABA‐A pathway gene to levels similar to those in the ICSI group.
Conclusion
Based on our results, decreased gene expression is a result of the microinjection manipulation performed during ICSI.
(A) The red points represent genes that were upregulated based on a |fold change|> 1.0 and a corrected P‐value of |
| doi_str_mv | 10.1002/jcla.23584 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7843277</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2444602607</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4074-ac45a33721b8f6c47486bf08d9977e40f2b29b379afe9a754dee95bfca11abf03</originalsourceid><addsrcrecordid>eNp9kUGO0zAUhi0EYsrAhgMgS2wQUgbbcWJ7gxQqGEBFbGBtOe5L6yqJg53MkB1H4ApcjZPgTIcKWLCyrPf50-_3I_SYkgtKCHtxsK25YHkh-R20okTJjElW3EUrIqXIJKH5GXoQ44EQIhUt76OznKmCspKs0I8Pzgbv-gPY0fked6Z3w9Sam8sWbAATIeJxDxi-DgFiXAa-wZfVq-rnt-8VDmBhGH3A0e1607p-hwcz7q_NjHfQp7cuWf0Uk6Crw-xj0gZ3BVs8xQV2_RiMnUc_tCZ2zuI4QOjwKdJDdK8xbYRHt-c5-vzm9af122zz8fLdutpklhPBM2N5YfJcMFrLprRccFnWDZFbpYQAThpWM1XnQpkGlBEF3wKoom6sodQkMD9HL4_eYao72FpYcrV6CK4zYdbeOP33pHd7vfNXWkieMyGS4NmtIPgvE8RRdy5aaFvTQ_q_ZpzzkqStL-jTf9CDn0La3kJJxgqiyjxRz49UaijGAM0pDCV6aV4vzeub5hP85M_4J_R31QmgR-DatTD_R6XfrzfVUfoLN92_dw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2482250963</pqid></control><display><type>article</type><title>Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection</title><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Zhang, Zili ; Wang, Ting ; Huang, Juanhua ; Huang, Yonghan ; Zhang, Qingxue</creator><creatorcontrib>Zhang, Zili ; Wang, Ting ; Huang, Juanhua ; Huang, Yonghan ; Zhang, Qingxue</creatorcontrib><description>Background
The GABA‐A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development.
Methods
To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression dataset, GSE23009, was obtained from the Gene Expression Omnibus database. Genes with differential expression were identified using the R software package, and RT‐qPCR was performed to confirm the microarray results.
Results
Mouse blastocysts derived from ICSI fertilization had decreased expression of GABA‐A receptor signaling pathway genes. However, the mechanisms underlying these changes were not elucidated. The gene expression of the GABA‐A pathway was not significantly different between blastocysts obtained from IVF and in vivo fertilization. However, microinjection after IVF significantly reduced the expression of the GABA‐A pathway gene to levels similar to those in the ICSI group.
Conclusion
Based on our results, decreased gene expression is a result of the microinjection manipulation performed during ICSI.
(A) The red points represent genes that were upregulated based on a |fold change|> 1.0 and a corrected P‐value of < .05. The blue points represent downregulated genes screened based on a |fold change| < 1.0 and a corrected P‐value of < .05. The black points represent genes with no significant differences in expression. (B) The GO analysis divided DEGs into three functional groups, including molecular function, biological processes, and cellular components.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.23584</identifier><identifier>PMID: 32951260</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Apoptosis ; Blastocysts ; Congenital defects ; Datasets ; DNA damage ; DNA microarrays ; Embryogenesis ; embryonic development ; Embryos ; Fertilization ; GABA‐A receptor signaling pathway ; Gene expression ; Genetic engineering ; Homeostasis ; In vitro fertilization ; Infertility ; intracytoplasmic sperm injection ; Microinjection ; Signal transduction ; Software ; Sperm ; γ-Aminobutyric acid</subject><ispartof>Journal of clinical laboratory analysis, 2021-01, Vol.35 (1), p.e23584-n/a</ispartof><rights>2020 The Authors. published by Wiley Periodicals LLC</rights><rights>2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4074-ac45a33721b8f6c47486bf08d9977e40f2b29b379afe9a754dee95bfca11abf03</cites><orcidid>0000-0001-5402-4604</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843277/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843277/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32951260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zili</creatorcontrib><creatorcontrib>Wang, Ting</creatorcontrib><creatorcontrib>Huang, Juanhua</creatorcontrib><creatorcontrib>Huang, Yonghan</creatorcontrib><creatorcontrib>Zhang, Qingxue</creatorcontrib><title>Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection</title><title>Journal of clinical laboratory analysis</title><addtitle>J Clin Lab Anal</addtitle><description>Background
The GABA‐A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development.
Methods
To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression dataset, GSE23009, was obtained from the Gene Expression Omnibus database. Genes with differential expression were identified using the R software package, and RT‐qPCR was performed to confirm the microarray results.
Results
Mouse blastocysts derived from ICSI fertilization had decreased expression of GABA‐A receptor signaling pathway genes. However, the mechanisms underlying these changes were not elucidated. The gene expression of the GABA‐A pathway was not significantly different between blastocysts obtained from IVF and in vivo fertilization. However, microinjection after IVF significantly reduced the expression of the GABA‐A pathway gene to levels similar to those in the ICSI group.
Conclusion
Based on our results, decreased gene expression is a result of the microinjection manipulation performed during ICSI.
(A) The red points represent genes that were upregulated based on a |fold change|> 1.0 and a corrected P‐value of < .05. The blue points represent downregulated genes screened based on a |fold change| < 1.0 and a corrected P‐value of < .05. The black points represent genes with no significant differences in expression. (B) The GO analysis divided DEGs into three functional groups, including molecular function, biological processes, and cellular components.</description><subject>Apoptosis</subject><subject>Blastocysts</subject><subject>Congenital defects</subject><subject>Datasets</subject><subject>DNA damage</subject><subject>DNA microarrays</subject><subject>Embryogenesis</subject><subject>embryonic development</subject><subject>Embryos</subject><subject>Fertilization</subject><subject>GABA‐A receptor signaling pathway</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Homeostasis</subject><subject>In vitro fertilization</subject><subject>Infertility</subject><subject>intracytoplasmic sperm injection</subject><subject>Microinjection</subject><subject>Signal transduction</subject><subject>Software</subject><subject>Sperm</subject><subject>γ-Aminobutyric acid</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUGO0zAUhi0EYsrAhgMgS2wQUgbbcWJ7gxQqGEBFbGBtOe5L6yqJg53MkB1H4ApcjZPgTIcKWLCyrPf50-_3I_SYkgtKCHtxsK25YHkh-R20okTJjElW3EUrIqXIJKH5GXoQ44EQIhUt76OznKmCspKs0I8Pzgbv-gPY0fked6Z3w9Sam8sWbAATIeJxDxi-DgFiXAa-wZfVq-rnt-8VDmBhGH3A0e1607p-hwcz7q_NjHfQp7cuWf0Uk6Crw-xj0gZ3BVs8xQV2_RiMnUc_tCZ2zuI4QOjwKdJDdK8xbYRHt-c5-vzm9af122zz8fLdutpklhPBM2N5YfJcMFrLprRccFnWDZFbpYQAThpWM1XnQpkGlBEF3wKoom6sodQkMD9HL4_eYao72FpYcrV6CK4zYdbeOP33pHd7vfNXWkieMyGS4NmtIPgvE8RRdy5aaFvTQ_q_ZpzzkqStL-jTf9CDn0La3kJJxgqiyjxRz49UaijGAM0pDCV6aV4vzeub5hP85M_4J_R31QmgR-DatTD_R6XfrzfVUfoLN92_dw</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Zhang, Zili</creator><creator>Wang, Ting</creator><creator>Huang, Juanhua</creator><creator>Huang, Yonghan</creator><creator>Zhang, Qingxue</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5402-4604</orcidid></search><sort><creationdate>202101</creationdate><title>Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection</title><author>Zhang, Zili ; Wang, Ting ; Huang, Juanhua ; Huang, Yonghan ; Zhang, Qingxue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4074-ac45a33721b8f6c47486bf08d9977e40f2b29b379afe9a754dee95bfca11abf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Apoptosis</topic><topic>Blastocysts</topic><topic>Congenital defects</topic><topic>Datasets</topic><topic>DNA damage</topic><topic>DNA microarrays</topic><topic>Embryogenesis</topic><topic>embryonic development</topic><topic>Embryos</topic><topic>Fertilization</topic><topic>GABA‐A receptor signaling pathway</topic><topic>Gene expression</topic><topic>Genetic engineering</topic><topic>Homeostasis</topic><topic>In vitro fertilization</topic><topic>Infertility</topic><topic>intracytoplasmic sperm injection</topic><topic>Microinjection</topic><topic>Signal transduction</topic><topic>Software</topic><topic>Sperm</topic><topic>γ-Aminobutyric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zili</creatorcontrib><creatorcontrib>Wang, Ting</creatorcontrib><creatorcontrib>Huang, Juanhua</creatorcontrib><creatorcontrib>Huang, Yonghan</creatorcontrib><creatorcontrib>Zhang, Qingxue</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zili</au><au>Wang, Ting</au><au>Huang, Juanhua</au><au>Huang, Yonghan</au><au>Zhang, Qingxue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J Clin Lab Anal</addtitle><date>2021-01</date><risdate>2021</risdate><volume>35</volume><issue>1</issue><spage>e23584</spage><epage>n/a</epage><pages>e23584-n/a</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Background
The GABA‐A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development.
Methods
To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression dataset, GSE23009, was obtained from the Gene Expression Omnibus database. Genes with differential expression were identified using the R software package, and RT‐qPCR was performed to confirm the microarray results.
Results
Mouse blastocysts derived from ICSI fertilization had decreased expression of GABA‐A receptor signaling pathway genes. However, the mechanisms underlying these changes were not elucidated. The gene expression of the GABA‐A pathway was not significantly different between blastocysts obtained from IVF and in vivo fertilization. However, microinjection after IVF significantly reduced the expression of the GABA‐A pathway gene to levels similar to those in the ICSI group.
Conclusion
Based on our results, decreased gene expression is a result of the microinjection manipulation performed during ICSI.
(A) The red points represent genes that were upregulated based on a |fold change|> 1.0 and a corrected P‐value of < .05. The blue points represent downregulated genes screened based on a |fold change| < 1.0 and a corrected P‐value of < .05. The black points represent genes with no significant differences in expression. (B) The GO analysis divided DEGs into three functional groups, including molecular function, biological processes, and cellular components.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>32951260</pmid><doi>10.1002/jcla.23584</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5402-4604</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Apoptosis Blastocysts Congenital defects Datasets DNA damage DNA microarrays Embryogenesis embryonic development Embryos Fertilization GABA‐A receptor signaling pathway Gene expression Genetic engineering Homeostasis In vitro fertilization Infertility intracytoplasmic sperm injection Microinjection Signal transduction Software Sperm γ-Aminobutyric acid |
| title | Microinjection manipulation decreases the expression of GABA‐A receptor signaling pathway genes in mouse embryos derived using intracytoplasmic sperm injection |
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