Effects of N‐Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats
N‐acetylcysteine (NAC) has been found to enhance the protective ability of cells to counter balance oxidative stress and inflammation. To investigate the effects of dietary NAC supplementation on the reproductive performance of goats, the reproductive performance and endometrial transcriptome of goa...
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| description | N‐acetylcysteine (NAC) has been found to enhance the protective ability of cells to counter balance oxidative stress and inflammation. To investigate the effects of dietary NAC supplementation on the reproductive performance of goats, the reproductive performance and endometrial transcriptome of goats fed with diets with NAC (NAC group) and without NAC supplementation (control group) were compared. Results showed that the goats fed with 0.03% and 0.05% NAC had similar litter size, birth weight, nitric oxide (NO), sex hormones and amino acids levels compared with the goats of the control group. However, feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of goats. The goats of the 0.07% NAC group presented increased levels of NO relative to the control group, but their sex hormones and amino acids showed no differences. Comparative transcriptome analysis identified 207 differentially expressed genes (DEGs) in the endometrium between the control and the 0.07% NAC groups. These DEGs included 146 upregulated genes and 61 downregulated genes in the 0.07% NAC group. They were primarily involved in the cellular response to toxic substances, oxidoreductase activity, immune receptor activity, signalling receptor binding, cytokine–cytokine receptor interactions, PI3K‐Akt signalling pathway and PPAR signalling pathway. In conclusion, results showed that dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of goat embryos at the early pregnancy stage. Such positive outcome might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.
Feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of Nubian ewes. Dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of ewe embryos at the early pregnancy stage might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium. |
| doi_str_mv | 10.1002/vms3.338 |
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Feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of Nubian ewes. Dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of ewe embryos at the early pregnancy stage might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.</description><identifier>ISSN: 2053-1095</identifier><identifier>EISSN: 2053-1095</identifier><identifier>DOI: 10.1002/vms3.338</identifier><identifier>PMID: 32812379</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>1-Phosphatidylinositol 3-kinase ; Acetylcysteine ; AKT protein ; Amino acids ; Biotechnology industry ; Birth weight ; Diet ; Dietary supplements ; embryo survival ; Embryos ; Endometrium ; Gene expression ; Gestation ; Goats ; Hormones, Sex ; Inflammation ; Litter size ; Nitric oxide ; Nitrogen ; N‐acetylcysteine ; oestrous synchronization ; Original ; Oxidative stress ; Oxidoreductase ; Peroxisome proliferator-activated receptors ; Pregnancy ; Ribonucleic acid ; RNA ; RNA sequencing ; RNA‐Seq ; Sex hormones ; Signal transduction ; Transcriptomes ; Vagina</subject><ispartof>Veterinary Medicine and Science, 2021-01, Vol.7 (1), p.156-163</ispartof><rights>2020 The Authors. Published by John Wiley & Sons Ltd</rights><rights>2020 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5058-a794ab691ddf15b451bcb1a47dadd4132718c22fcadee389dd7a74af8f191f693</citedby><cites>FETCH-LOGICAL-c5058-a794ab691ddf15b451bcb1a47dadd4132718c22fcadee389dd7a74af8f191f693</cites><orcidid>0000-0002-7999-8752</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840200/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840200/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32812379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Jinhong</creatorcontrib><creatorcontrib>Ao, Zheng</creatorcontrib><creatorcontrib>Duan, Zhiqiang</creatorcontrib><creatorcontrib>Ao, Ye</creatorcontrib><creatorcontrib>Wei, Shinan</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Chen, Xiang</creatorcontrib><title>Effects of N‐Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats</title><title>Veterinary Medicine and Science</title><addtitle>Vet Med Sci</addtitle><description>N‐acetylcysteine (NAC) has been found to enhance the protective ability of cells to counter balance oxidative stress and inflammation. To investigate the effects of dietary NAC supplementation on the reproductive performance of goats, the reproductive performance and endometrial transcriptome of goats fed with diets with NAC (NAC group) and without NAC supplementation (control group) were compared. Results showed that the goats fed with 0.03% and 0.05% NAC had similar litter size, birth weight, nitric oxide (NO), sex hormones and amino acids levels compared with the goats of the control group. However, feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of goats. The goats of the 0.07% NAC group presented increased levels of NO relative to the control group, but their sex hormones and amino acids showed no differences. Comparative transcriptome analysis identified 207 differentially expressed genes (DEGs) in the endometrium between the control and the 0.07% NAC groups. These DEGs included 146 upregulated genes and 61 downregulated genes in the 0.07% NAC group. They were primarily involved in the cellular response to toxic substances, oxidoreductase activity, immune receptor activity, signalling receptor binding, cytokine–cytokine receptor interactions, PI3K‐Akt signalling pathway and PPAR signalling pathway. In conclusion, results showed that dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of goat embryos at the early pregnancy stage. Such positive outcome might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.
Feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of Nubian ewes. Dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of ewe embryos at the early pregnancy stage might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Acetylcysteine</subject><subject>AKT protein</subject><subject>Amino acids</subject><subject>Biotechnology industry</subject><subject>Birth weight</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>embryo survival</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Gene expression</subject><subject>Gestation</subject><subject>Goats</subject><subject>Hormones, Sex</subject><subject>Inflammation</subject><subject>Litter size</subject><subject>Nitric oxide</subject><subject>Nitrogen</subject><subject>N‐acetylcysteine</subject><subject>oestrous synchronization</subject><subject>Original</subject><subject>Oxidative stress</subject><subject>Oxidoreductase</subject><subject>Peroxisome proliferator-activated receptors</subject><subject>Pregnancy</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>RNA‐Seq</subject><subject>Sex hormones</subject><subject>Signal transduction</subject><subject>Transcriptomes</subject><subject>Vagina</subject><issn>2053-1095</issn><issn>2053-1095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kU1uFDEQhVuIiERJJE6ALLFhkZ74p912b5BGUSBISZD421puuzxxNG0PdvfA7DgCZ8xJ8GRCFBasbJVfffXKr6peEjwjGNPT9ZDZjDH5rDqgmLOa4I4_f3Lfr45zvsUYE94IxsWLap9RSSgT3UGVzp0DM2YUHbq--_V7bmDcLM0mj-ADoBjQeAMowSpFO5nRrwGtILmYBh0MnKD401t9X85jgpyRDhZ9up6jDN8nCMaHxT166r0OaBH1mI-qPaeXGY4fzsPq67vzL2cX9eXH9x_O5pe14ZjLWouu0X3bEWsd4X3DSW96ohthtbUNYVQQaSh1RlsAJjtrhRaNdtKRjri2Y4fV2x13NfUDWANhTHqpVskPOm1U1F79-xL8jVrEtRKywRTjAnj9AEixLJNHdRunFIpnRZvygW3LO1lUs51qoZegfHCxwIze2hq8iQGcL_W5YIK2uJNNaXizazAp5pzAPVoiWG0TVdtEVUm0SF89XeFR-De_Iqh3gh9lyOa_IPXt6jPbAv8ATa-tkQ</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Luo, Jinhong</creator><creator>Ao, Zheng</creator><creator>Duan, Zhiqiang</creator><creator>Ao, Ye</creator><creator>Wei, Shinan</creator><creator>Chen, Wei</creator><creator>Chen, Xiang</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7999-8752</orcidid></search><sort><creationdate>202101</creationdate><title>Effects of N‐Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats</title><author>Luo, Jinhong ; Ao, Zheng ; Duan, Zhiqiang ; Ao, Ye ; Wei, Shinan ; Chen, Wei ; Chen, Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5058-a794ab691ddf15b451bcb1a47dadd4132718c22fcadee389dd7a74af8f191f693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Acetylcysteine</topic><topic>AKT protein</topic><topic>Amino acids</topic><topic>Biotechnology industry</topic><topic>Birth weight</topic><topic>Diet</topic><topic>Dietary supplements</topic><topic>embryo survival</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Gene expression</topic><topic>Gestation</topic><topic>Goats</topic><topic>Hormones, Sex</topic><topic>Inflammation</topic><topic>Litter size</topic><topic>Nitric oxide</topic><topic>Nitrogen</topic><topic>N‐acetylcysteine</topic><topic>oestrous synchronization</topic><topic>Original</topic><topic>Oxidative stress</topic><topic>Oxidoreductase</topic><topic>Peroxisome proliferator-activated receptors</topic><topic>Pregnancy</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>RNA‐Seq</topic><topic>Sex hormones</topic><topic>Signal transduction</topic><topic>Transcriptomes</topic><topic>Vagina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jinhong</creatorcontrib><creatorcontrib>Ao, Zheng</creatorcontrib><creatorcontrib>Duan, Zhiqiang</creatorcontrib><creatorcontrib>Ao, Ye</creatorcontrib><creatorcontrib>Wei, Shinan</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Chen, Xiang</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary Medicine and Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jinhong</au><au>Ao, Zheng</au><au>Duan, Zhiqiang</au><au>Ao, Ye</au><au>Wei, Shinan</au><au>Chen, Wei</au><au>Chen, Xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of N‐Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats</atitle><jtitle>Veterinary Medicine and Science</jtitle><addtitle>Vet Med Sci</addtitle><date>2021-01</date><risdate>2021</risdate><volume>7</volume><issue>1</issue><spage>156</spage><epage>163</epage><pages>156-163</pages><issn>2053-1095</issn><eissn>2053-1095</eissn><abstract>N‐acetylcysteine (NAC) has been found to enhance the protective ability of cells to counter balance oxidative stress and inflammation. To investigate the effects of dietary NAC supplementation on the reproductive performance of goats, the reproductive performance and endometrial transcriptome of goats fed with diets with NAC (NAC group) and without NAC supplementation (control group) were compared. Results showed that the goats fed with 0.03% and 0.05% NAC had similar litter size, birth weight, nitric oxide (NO), sex hormones and amino acids levels compared with the goats of the control group. However, feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of goats. The goats of the 0.07% NAC group presented increased levels of NO relative to the control group, but their sex hormones and amino acids showed no differences. Comparative transcriptome analysis identified 207 differentially expressed genes (DEGs) in the endometrium between the control and the 0.07% NAC groups. These DEGs included 146 upregulated genes and 61 downregulated genes in the 0.07% NAC group. They were primarily involved in the cellular response to toxic substances, oxidoreductase activity, immune receptor activity, signalling receptor binding, cytokine–cytokine receptor interactions, PI3K‐Akt signalling pathway and PPAR signalling pathway. In conclusion, results showed that dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of goat embryos at the early pregnancy stage. Such positive outcome might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.
Feeding with 0.07% NAC supplementation from day 0 to day 30 of gestation remarkably increased the litter size of Nubian ewes. Dietary 0.07% NAC supplementation exerted a beneficial effect on the survival of ewe embryos at the early pregnancy stage might be due to the increased NO production and affected expression of genes involved in the anti‐inflammation pathways of the endometrium.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>32812379</pmid><doi>10.1002/vms3.338</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7999-8752</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase Acetylcysteine AKT protein Amino acids Biotechnology industry Birth weight Diet Dietary supplements embryo survival Embryos Endometrium Gene expression Gestation Goats Hormones, Sex Inflammation Litter size Nitric oxide Nitrogen N‐acetylcysteine oestrous synchronization Original Oxidative stress Oxidoreductase Peroxisome proliferator-activated receptors Pregnancy Ribonucleic acid RNA RNA sequencing RNA‐Seq Sex hormones Signal transduction Transcriptomes Vagina |
| title | Effects of N‐Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats |
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