Suitcase Lab for Rapid Detection of SARS-CoV‑2 Based on Recombinase Polymerase Amplification Assay

In March 2020, the SARS-CoV-2 virus outbreak was declared as a world pandemic by the World Health Organization (WHO). The only measures for controlling the outbreak are testing and isolation of infected cases. Molecular real-time polymerase chain reaction (PCR) assays are very sensitive but require...

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Veröffentlicht in:	Analytical chemistry (Washington) 2021-02, Vol.93 (4), p.2627-2634
Hauptverfasser:	El Wahed, Ahmed Abd, Patel, Pranav, Maier, Melanie, Pietsch, Corinna, Rüster, Dana, Böhlken-Fascher, Susanne, Kissenkötter, Jonas, Behrmann, Ole, Frimpong, Michael, Diagne, Moussa Moïse, Faye, Martin, Dia, Ndongo, Shalaby, Mohamed A, Amer, Haitham, Elgamal, Mahmoud, Zaki, Ali, Ismail, Ghada, Kaiser, Marco, Corman, Victor M, Niedrig, Matthias, Landt, Olfert, Faye, Ousmane, Sall, Amadou A, Hufert, Frank T, Truyen, Uwe, Liebert, Uwe G, Weidmann, Manfred
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Assaying Chemistry COVID-19 COVID-19 - diagnosis COVID-19 - virology DNA-directed RNA polymerase Humans Laboratories Molecular chains Nucleic acids Nucleocapsids Outbreaks Pandemics Polymerase chain reaction Proteins Real time Real-Time Polymerase Chain Reaction - methods Recombinase Recombinases - metabolism Respiratory diseases Reverse transcription Ribonucleic acid RNA RNA polymerase RNA-directed RNA polymerase SARS-CoV-2 - isolation & purification Sensitivity and Specificity Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Viral diseases Viral envelope proteins Viruses
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