Comparative research on nucleocapsid and spike glycoprotein as the rapid immunodetection targets of COVID-19 and establishment of immunoassay strips

•The monoclonal antibodies against S protein of COVID-19 have cross-reaction with HCoV-OC43, HCoV-NL63 and HCoV-HKU1.•The N protein Ag-mAb system is more specific and accurate, suitable for immunoassay.•N protein strips we prepared can quickly and easily screen the swab samples within 11 min with 93...

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