Wilms' Tumor 1 (WT1): A Novel Immunomarker of Dermatofibrosarcoma Protuberans-An Immunohistochemical Study on a Series of 114 Cases of Bland-Looking Mesenchymal Spindle Cell Lesions of the Dermis/Subcutaneous Tissues
to investigate the immunohistochemical expression and distribution of Wilms' tumor 1 (WT1) (transcription factor produced by the tumor suppressor gene of the same name) in a series of 114 cases of bland-looking mesenchymal spindle cell lesions of the dermis/subcutaneous tissues to establish whe...
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| Veröffentlicht in: | Cancers 2021-01, Vol.13 (2), p.252 |
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| creator | Piombino, Eliana Broggi, Giuseppe Barbareschi, Mattia Castorina, Sergio Parenti, Rosalba Bartoloni, Giovanni Salvatorelli, Lucia Magro, Gaetano |
| description | to investigate the immunohistochemical expression and distribution of Wilms' tumor 1 (WT1) (transcription factor produced by the tumor suppressor gene of the same name) in a series of 114 cases of bland-looking mesenchymal spindle cell lesions of the dermis/subcutaneous tissues to establish whether this immunomarker is differentially expressed in dermatofibrosarcoma protuberans (DFSP) versus its potential morphological mimickers.
This retrospective multi-centric immunohistochemical study included 57 DFSP cases, 15 dermatofibromas, 5 deep fibrous histiocytomas, 8 neurofibromas, 5 spindle cell lipomas, 8 dermal scars, 6 nodular fasciitis, 5 cutaneous leiomyomas and 5 solitary fibrous tumors. Among the 57 DFSP cases, 11 were recurrent lesions; 2 non-recurrent cases exhibited an additional "
" overgrowth and 1 recurrent and 2 primary tumors contained a minority of "
" components.
Most DFSP (95% of cases) exhibited cytoplasmic staining for WT1; 11/11 residual/recurrent tumors showed diffuse and strong WT1 cytoplasmic immunoreactivity; apart from neurofibromas, WT1 expression was lacking in all the other cases studied.
The cytoplasmic expression of WT1 may be exploitable as a complementary diagnostic immunomarker to CD34 in confirming the diagnosis of DFSP and to better evaluate the residual/recurrent tumor component. |
| doi_str_mv | 10.3390/cancers13020252 |
| format | Article |
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This retrospective multi-centric immunohistochemical study included 57 DFSP cases, 15 dermatofibromas, 5 deep fibrous histiocytomas, 8 neurofibromas, 5 spindle cell lipomas, 8 dermal scars, 6 nodular fasciitis, 5 cutaneous leiomyomas and 5 solitary fibrous tumors. Among the 57 DFSP cases, 11 were recurrent lesions; 2 non-recurrent cases exhibited an additional "
" overgrowth and 1 recurrent and 2 primary tumors contained a minority of "
" components.
Most DFSP (95% of cases) exhibited cytoplasmic staining for WT1; 11/11 residual/recurrent tumors showed diffuse and strong WT1 cytoplasmic immunoreactivity; apart from neurofibromas, WT1 expression was lacking in all the other cases studied.
The cytoplasmic expression of WT1 may be exploitable as a complementary diagnostic immunomarker to CD34 in confirming the diagnosis of DFSP and to better evaluate the residual/recurrent tumor component.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers13020252</identifier><identifier>PMID: 33445443</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Age ; Biopsy ; CD34 antigen ; Cytoplasm ; Dermatofibrosarcoma protuberans ; Dermis ; Fasciitis ; Fibroids ; Immunohistochemistry ; Immunoreactivity ; Lesions ; Mesenchyme ; Morphology ; Musculoskeletal system ; Proteins ; Sarcoma ; Transcription factors ; Tumor suppressor genes ; Tumors</subject><ispartof>Cancers, 2021-01, Vol.13 (2), p.252</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-544c21f04acbc219739f075b5c1eebdf5917fc0cbfc8fc76ec81bc4ca1efd8f23</citedby><cites>FETCH-LOGICAL-c421t-544c21f04acbc219739f075b5c1eebdf5917fc0cbfc8fc76ec81bc4ca1efd8f23</cites><orcidid>0000-0003-2576-6523 ; 0000-0002-1614-4696</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826654/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826654/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33445443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Piombino, Eliana</creatorcontrib><creatorcontrib>Broggi, Giuseppe</creatorcontrib><creatorcontrib>Barbareschi, Mattia</creatorcontrib><creatorcontrib>Castorina, Sergio</creatorcontrib><creatorcontrib>Parenti, Rosalba</creatorcontrib><creatorcontrib>Bartoloni, Giovanni</creatorcontrib><creatorcontrib>Salvatorelli, Lucia</creatorcontrib><creatorcontrib>Magro, Gaetano</creatorcontrib><title>Wilms' Tumor 1 (WT1): A Novel Immunomarker of Dermatofibrosarcoma Protuberans-An Immunohistochemical Study on a Series of 114 Cases of Bland-Looking Mesenchymal Spindle Cell Lesions of the Dermis/Subcutaneous Tissues</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>to investigate the immunohistochemical expression and distribution of Wilms' tumor 1 (WT1) (transcription factor produced by the tumor suppressor gene of the same name) in a series of 114 cases of bland-looking mesenchymal spindle cell lesions of the dermis/subcutaneous tissues to establish whether this immunomarker is differentially expressed in dermatofibrosarcoma protuberans (DFSP) versus its potential morphological mimickers.
This retrospective multi-centric immunohistochemical study included 57 DFSP cases, 15 dermatofibromas, 5 deep fibrous histiocytomas, 8 neurofibromas, 5 spindle cell lipomas, 8 dermal scars, 6 nodular fasciitis, 5 cutaneous leiomyomas and 5 solitary fibrous tumors. Among the 57 DFSP cases, 11 were recurrent lesions; 2 non-recurrent cases exhibited an additional "
" overgrowth and 1 recurrent and 2 primary tumors contained a minority of "
" components.
Most DFSP (95% of cases) exhibited cytoplasmic staining for WT1; 11/11 residual/recurrent tumors showed diffuse and strong WT1 cytoplasmic immunoreactivity; apart from neurofibromas, WT1 expression was lacking in all the other cases studied.
The cytoplasmic expression of WT1 may be exploitable as a complementary diagnostic immunomarker to CD34 in confirming the diagnosis of DFSP and to better evaluate the residual/recurrent tumor component.</description><subject>Age</subject><subject>Biopsy</subject><subject>CD34 antigen</subject><subject>Cytoplasm</subject><subject>Dermatofibrosarcoma protuberans</subject><subject>Dermis</subject><subject>Fasciitis</subject><subject>Fibroids</subject><subject>Immunohistochemistry</subject><subject>Immunoreactivity</subject><subject>Lesions</subject><subject>Mesenchyme</subject><subject>Morphology</subject><subject>Musculoskeletal system</subject><subject>Proteins</subject><subject>Sarcoma</subject><subject>Transcription factors</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdUstuFDEQHCEQiULO3JAlDoTDsH7Ni0OkZQkQaXlIuyjHkaennXUyYy_2TKT903wO3gdRiC9uq6tKVe1OkteMfhCiohNQFtAHJiinPOPPkmNOC57meSWfP6qPktMQbmg8QrAiL14mR0JImUkpjpP7K9P14R1Zjr3zhJGzqyV7_5FMyQ93hx257PvRul75W_TEafIZfa8Gp03jXVAeYov88m4YG_TKhnRqD5SVCYODFfYGVEcWw9huiLNEkQV6g2GrxZgkMxX2j0-dsm06d-7W2GvyHQNaWG36LXdtbNshmWHXkTkG4-yOMaxwZ8eEyWJsYByURTcGsjQhjBheJS-06gKeHu6T5PeXi-XsWzr_-fVyNp2nIDkb0jgF4ExTqaCJRVWIStMiazJgiE2rs4oVGig0GkoNRY5QsgYkKIa6LTUXJ8n5Xnc9Nj22gHbwqqvX3sSpbWqnTP1_x5pVfe3u6qLkeZ7JKHB2EPDuTzQ-1DESxLD7PDWXRZlVNOMiQt8-gd640dsYb4cSWV7QKqImexTEPwoe9YMZRuvt4tRPFicy3jzO8ID_tybiL4bBxHM</recordid><startdate>20210112</startdate><enddate>20210112</enddate><creator>Piombino, Eliana</creator><creator>Broggi, Giuseppe</creator><creator>Barbareschi, Mattia</creator><creator>Castorina, Sergio</creator><creator>Parenti, Rosalba</creator><creator>Bartoloni, Giovanni</creator><creator>Salvatorelli, Lucia</creator><creator>Magro, Gaetano</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2576-6523</orcidid><orcidid>https://orcid.org/0000-0002-1614-4696</orcidid></search><sort><creationdate>20210112</creationdate><title>Wilms' Tumor 1 (WT1): A Novel Immunomarker of Dermatofibrosarcoma Protuberans-An Immunohistochemical Study on a Series of 114 Cases of Bland-Looking Mesenchymal Spindle Cell Lesions of the Dermis/Subcutaneous Tissues</title><author>Piombino, Eliana ; Broggi, Giuseppe ; Barbareschi, Mattia ; Castorina, Sergio ; Parenti, Rosalba ; Bartoloni, Giovanni ; Salvatorelli, Lucia ; Magro, Gaetano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-544c21f04acbc219739f075b5c1eebdf5917fc0cbfc8fc76ec81bc4ca1efd8f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Age</topic><topic>Biopsy</topic><topic>CD34 antigen</topic><topic>Cytoplasm</topic><topic>Dermatofibrosarcoma protuberans</topic><topic>Dermis</topic><topic>Fasciitis</topic><topic>Fibroids</topic><topic>Immunohistochemistry</topic><topic>Immunoreactivity</topic><topic>Lesions</topic><topic>Mesenchyme</topic><topic>Morphology</topic><topic>Musculoskeletal system</topic><topic>Proteins</topic><topic>Sarcoma</topic><topic>Transcription factors</topic><topic>Tumor suppressor genes</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Piombino, Eliana</creatorcontrib><creatorcontrib>Broggi, Giuseppe</creatorcontrib><creatorcontrib>Barbareschi, Mattia</creatorcontrib><creatorcontrib>Castorina, Sergio</creatorcontrib><creatorcontrib>Parenti, Rosalba</creatorcontrib><creatorcontrib>Bartoloni, Giovanni</creatorcontrib><creatorcontrib>Salvatorelli, Lucia</creatorcontrib><creatorcontrib>Magro, Gaetano</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Piombino, Eliana</au><au>Broggi, Giuseppe</au><au>Barbareschi, Mattia</au><au>Castorina, Sergio</au><au>Parenti, Rosalba</au><au>Bartoloni, Giovanni</au><au>Salvatorelli, Lucia</au><au>Magro, Gaetano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wilms' Tumor 1 (WT1): A Novel Immunomarker of Dermatofibrosarcoma Protuberans-An Immunohistochemical Study on a Series of 114 Cases of Bland-Looking Mesenchymal Spindle Cell Lesions of the Dermis/Subcutaneous Tissues</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2021-01-12</date><risdate>2021</risdate><volume>13</volume><issue>2</issue><spage>252</spage><pages>252-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>to investigate the immunohistochemical expression and distribution of Wilms' tumor 1 (WT1) (transcription factor produced by the tumor suppressor gene of the same name) in a series of 114 cases of bland-looking mesenchymal spindle cell lesions of the dermis/subcutaneous tissues to establish whether this immunomarker is differentially expressed in dermatofibrosarcoma protuberans (DFSP) versus its potential morphological mimickers.
This retrospective multi-centric immunohistochemical study included 57 DFSP cases, 15 dermatofibromas, 5 deep fibrous histiocytomas, 8 neurofibromas, 5 spindle cell lipomas, 8 dermal scars, 6 nodular fasciitis, 5 cutaneous leiomyomas and 5 solitary fibrous tumors. Among the 57 DFSP cases, 11 were recurrent lesions; 2 non-recurrent cases exhibited an additional "
" overgrowth and 1 recurrent and 2 primary tumors contained a minority of "
" components.
Most DFSP (95% of cases) exhibited cytoplasmic staining for WT1; 11/11 residual/recurrent tumors showed diffuse and strong WT1 cytoplasmic immunoreactivity; apart from neurofibromas, WT1 expression was lacking in all the other cases studied.
The cytoplasmic expression of WT1 may be exploitable as a complementary diagnostic immunomarker to CD34 in confirming the diagnosis of DFSP and to better evaluate the residual/recurrent tumor component.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33445443</pmid><doi>10.3390/cancers13020252</doi><orcidid>https://orcid.org/0000-0003-2576-6523</orcidid><orcidid>https://orcid.org/0000-0002-1614-4696</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Cancers, 2021-01, Vol.13 (2), p.252 |
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| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Age Biopsy CD34 antigen Cytoplasm Dermatofibrosarcoma protuberans Dermis Fasciitis Fibroids Immunohistochemistry Immunoreactivity Lesions Mesenchyme Morphology Musculoskeletal system Proteins Sarcoma Transcription factors Tumor suppressor genes Tumors |
| title | Wilms' Tumor 1 (WT1): A Novel Immunomarker of Dermatofibrosarcoma Protuberans-An Immunohistochemical Study on a Series of 114 Cases of Bland-Looking Mesenchymal Spindle Cell Lesions of the Dermis/Subcutaneous Tissues |
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