Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study
Lately, in orthodontic treatments, the use of transparent aligners for the correction of malocclusions has become prominent owing to their intrinsic advantages such as esthetics, comfort, and minimal maintenance. Attempts at improving upon this technology by varying various parameters to investigate...
Gespeichert in:
Veröffentlicht in: | Materials 2021-01, Vol.14 (2), p.324 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 2 |
container_start_page | 324 |
container_title | Materials |
container_volume | 14 |
creator | Seo, Jeong-Hee Eghan-Acquah, Emmanuel Kim, Min-Seok Lee, Jeong-Hyeon Jeong, Yong-Hoon Jung, Tae-Gon Hong, Mihee Kim, Won-Hyeon Kim, Bongju Lee, Sung-Jae |
description | Lately, in orthodontic treatments, the use of transparent aligners for the correction of malocclusions has become prominent owing to their intrinsic advantages such as esthetics, comfort, and minimal maintenance. Attempts at improving upon this technology by varying various parameters to investigate the effects on treatments have been carried out by several researchers. Here, we aimed to investigate the biomechanical and clinical effects of aligner thickness on stress distributions in the periodontal ligament and changes in the tooth's center of rotation. Dental finite element models comprising the cortical and cancellous bones, gingiva, teeth, and nonlinear viscoelastic periodontal ligaments were constructed, validated, and used together with aligner finite element models of different aligner thicknesses to achieve the goal of this study. The finite element analyses were conducted to simulate the actual orthodontic aligner treatment process for the correction of malocclusions by generating pre-stresses in the aligner and allowing the aligner stresses to relax to induce tooth movement. The results of the analyses showed that orthodontic treatment in lingual inclination and axial rotation with a 0.75 mm-thick aligner resulted in 6% and 0.03% higher principal stresses in the periodontal ligament than the same treatment using a 0.05 mm-thick aligner, respectively. Again, for both aligner thicknesses, the tooth's center of rotation moved lingually and towards the root direction in lingual inclination, and diagonally from the long axis of the tooth in axial rotation. Taken together, orthodontic treatment for simple malocclusions using transparent aligners of different thicknesses will produce a similar effect on the principal stresses in the periodontal ligament and similar changes in the tooth's center of rotation, as well as sufficient tooth movement. These findings provide orthodontists and researchers clinical and biomechanical evidence about the effect of transparent aligner thickness selection and its effect on orthodontic treatment. |
doi_str_mv | 10.3390/ma14020324 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7826543</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2477819731</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-4e22204a9cddcb714115e6cdce5411fbc00fced6ba56fd708d7b1f19596b9c143</originalsourceid><addsrcrecordid>eNpdkl1rFDEUhgdRbKm98QdIwBsRpiaTzEduhGXaWmGhYtfrIZOc2UnNJGuSKeyP87-Z3W3Xam5yIM_z8gZOlr0l-IJSjj9NgjBcYFqwF9kp4bzKCWfs5bP5JDsP4R6nQylpCv46O6GU0ZKV9Wn2u3XTRngR9QOghRVmG3RAbkB30UMISFsUR0DfwGunnI3CoKVeiwlsRMIq1KYB_E747mJKcfZJWTkXRySG3fOtj-Ne1xKtPIi49y9hA1Zpu0bJag0IjxZGr20SVqOWP20qkF9rqyOgKwN751jxLs5q-yZ7NQgT4PzxPst-XF-t2pt8efvla7tY5pLhKuYMiqLATHCplOxrwggpoZJKQpnGoZcYDxJU1YuyGlSNG1X3ZCC85FXPJWH0LPt8yN3M_QTJs9EL0228noTfdk7o7t8Xq8du7R66uimqktEU8OExwLtfM4TYTTpIMEZYcHPoClbXJSYNKxL6_j_03s0-fftANYTXlCTq44GS3oXgYTiWIbjbLUb3dzES_O55_SP6tAb0DwSlt64</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2477819731</pqid></control><display><type>article</type><title>Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study</title><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Seo, Jeong-Hee ; Eghan-Acquah, Emmanuel ; Kim, Min-Seok ; Lee, Jeong-Hyeon ; Jeong, Yong-Hoon ; Jung, Tae-Gon ; Hong, Mihee ; Kim, Won-Hyeon ; Kim, Bongju ; Lee, Sung-Jae</creator><creatorcontrib>Seo, Jeong-Hee ; Eghan-Acquah, Emmanuel ; Kim, Min-Seok ; Lee, Jeong-Hyeon ; Jeong, Yong-Hoon ; Jung, Tae-Gon ; Hong, Mihee ; Kim, Won-Hyeon ; Kim, Bongju ; Lee, Sung-Jae</creatorcontrib><description>Lately, in orthodontic treatments, the use of transparent aligners for the correction of malocclusions has become prominent owing to their intrinsic advantages such as esthetics, comfort, and minimal maintenance. Attempts at improving upon this technology by varying various parameters to investigate the effects on treatments have been carried out by several researchers. Here, we aimed to investigate the biomechanical and clinical effects of aligner thickness on stress distributions in the periodontal ligament and changes in the tooth's center of rotation. Dental finite element models comprising the cortical and cancellous bones, gingiva, teeth, and nonlinear viscoelastic periodontal ligaments were constructed, validated, and used together with aligner finite element models of different aligner thicknesses to achieve the goal of this study. The finite element analyses were conducted to simulate the actual orthodontic aligner treatment process for the correction of malocclusions by generating pre-stresses in the aligner and allowing the aligner stresses to relax to induce tooth movement. The results of the analyses showed that orthodontic treatment in lingual inclination and axial rotation with a 0.75 mm-thick aligner resulted in 6% and 0.03% higher principal stresses in the periodontal ligament than the same treatment using a 0.05 mm-thick aligner, respectively. Again, for both aligner thicknesses, the tooth's center of rotation moved lingually and towards the root direction in lingual inclination, and diagonally from the long axis of the tooth in axial rotation. Taken together, orthodontic treatment for simple malocclusions using transparent aligners of different thicknesses will produce a similar effect on the principal stresses in the periodontal ligament and similar changes in the tooth's center of rotation, as well as sufficient tooth movement. These findings provide orthodontists and researchers clinical and biomechanical evidence about the effect of transparent aligner thickness selection and its effect on orthodontic treatment.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma14020324</identifier><identifier>PMID: 33435457</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biomechanics ; Bones ; Dental care ; Design ; Enamel ; Finite element analysis ; Finite element method ; Inclination ; Ligaments ; Mathematical models ; Orthodontics ; Rotation ; Stress analysis ; Teeth ; Thickness</subject><ispartof>Materials, 2021-01, Vol.14 (2), p.324</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-4e22204a9cddcb714115e6cdce5411fbc00fced6ba56fd708d7b1f19596b9c143</citedby><cites>FETCH-LOGICAL-c406t-4e22204a9cddcb714115e6cdce5411fbc00fced6ba56fd708d7b1f19596b9c143</cites><orcidid>0000-0002-0011-6981 ; 0000-0002-4793-9125 ; 0000-0002-6702-1960 ; 0000-0001-6015-1482 ; 0000-0001-7309-5977 ; 0000-0002-9742-7945</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826543/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826543/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33435457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seo, Jeong-Hee</creatorcontrib><creatorcontrib>Eghan-Acquah, Emmanuel</creatorcontrib><creatorcontrib>Kim, Min-Seok</creatorcontrib><creatorcontrib>Lee, Jeong-Hyeon</creatorcontrib><creatorcontrib>Jeong, Yong-Hoon</creatorcontrib><creatorcontrib>Jung, Tae-Gon</creatorcontrib><creatorcontrib>Hong, Mihee</creatorcontrib><creatorcontrib>Kim, Won-Hyeon</creatorcontrib><creatorcontrib>Kim, Bongju</creatorcontrib><creatorcontrib>Lee, Sung-Jae</creatorcontrib><title>Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study</title><title>Materials</title><addtitle>Materials (Basel)</addtitle><description>Lately, in orthodontic treatments, the use of transparent aligners for the correction of malocclusions has become prominent owing to their intrinsic advantages such as esthetics, comfort, and minimal maintenance. Attempts at improving upon this technology by varying various parameters to investigate the effects on treatments have been carried out by several researchers. Here, we aimed to investigate the biomechanical and clinical effects of aligner thickness on stress distributions in the periodontal ligament and changes in the tooth's center of rotation. Dental finite element models comprising the cortical and cancellous bones, gingiva, teeth, and nonlinear viscoelastic periodontal ligaments were constructed, validated, and used together with aligner finite element models of different aligner thicknesses to achieve the goal of this study. The finite element analyses were conducted to simulate the actual orthodontic aligner treatment process for the correction of malocclusions by generating pre-stresses in the aligner and allowing the aligner stresses to relax to induce tooth movement. The results of the analyses showed that orthodontic treatment in lingual inclination and axial rotation with a 0.75 mm-thick aligner resulted in 6% and 0.03% higher principal stresses in the periodontal ligament than the same treatment using a 0.05 mm-thick aligner, respectively. Again, for both aligner thicknesses, the tooth's center of rotation moved lingually and towards the root direction in lingual inclination, and diagonally from the long axis of the tooth in axial rotation. Taken together, orthodontic treatment for simple malocclusions using transparent aligners of different thicknesses will produce a similar effect on the principal stresses in the periodontal ligament and similar changes in the tooth's center of rotation, as well as sufficient tooth movement. These findings provide orthodontists and researchers clinical and biomechanical evidence about the effect of transparent aligner thickness selection and its effect on orthodontic treatment.</description><subject>Biomechanics</subject><subject>Bones</subject><subject>Dental care</subject><subject>Design</subject><subject>Enamel</subject><subject>Finite element analysis</subject><subject>Finite element method</subject><subject>Inclination</subject><subject>Ligaments</subject><subject>Mathematical models</subject><subject>Orthodontics</subject><subject>Rotation</subject><subject>Stress analysis</subject><subject>Teeth</subject><subject>Thickness</subject><issn>1996-1944</issn><issn>1996-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkl1rFDEUhgdRbKm98QdIwBsRpiaTzEduhGXaWmGhYtfrIZOc2UnNJGuSKeyP87-Z3W3Xam5yIM_z8gZOlr0l-IJSjj9NgjBcYFqwF9kp4bzKCWfs5bP5JDsP4R6nQylpCv46O6GU0ZKV9Wn2u3XTRngR9QOghRVmG3RAbkB30UMISFsUR0DfwGunnI3CoKVeiwlsRMIq1KYB_E747mJKcfZJWTkXRySG3fOtj-Ne1xKtPIi49y9hA1Zpu0bJag0IjxZGr20SVqOWP20qkF9rqyOgKwN751jxLs5q-yZ7NQgT4PzxPst-XF-t2pt8efvla7tY5pLhKuYMiqLATHCplOxrwggpoZJKQpnGoZcYDxJU1YuyGlSNG1X3ZCC85FXPJWH0LPt8yN3M_QTJs9EL0228noTfdk7o7t8Xq8du7R66uimqktEU8OExwLtfM4TYTTpIMEZYcHPoClbXJSYNKxL6_j_03s0-fftANYTXlCTq44GS3oXgYTiWIbjbLUb3dzES_O55_SP6tAb0DwSlt64</recordid><startdate>20210109</startdate><enddate>20210109</enddate><creator>Seo, Jeong-Hee</creator><creator>Eghan-Acquah, Emmanuel</creator><creator>Kim, Min-Seok</creator><creator>Lee, Jeong-Hyeon</creator><creator>Jeong, Yong-Hoon</creator><creator>Jung, Tae-Gon</creator><creator>Hong, Mihee</creator><creator>Kim, Won-Hyeon</creator><creator>Kim, Bongju</creator><creator>Lee, Sung-Jae</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0011-6981</orcidid><orcidid>https://orcid.org/0000-0002-4793-9125</orcidid><orcidid>https://orcid.org/0000-0002-6702-1960</orcidid><orcidid>https://orcid.org/0000-0001-6015-1482</orcidid><orcidid>https://orcid.org/0000-0001-7309-5977</orcidid><orcidid>https://orcid.org/0000-0002-9742-7945</orcidid></search><sort><creationdate>20210109</creationdate><title>Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study</title><author>Seo, Jeong-Hee ; Eghan-Acquah, Emmanuel ; Kim, Min-Seok ; Lee, Jeong-Hyeon ; Jeong, Yong-Hoon ; Jung, Tae-Gon ; Hong, Mihee ; Kim, Won-Hyeon ; Kim, Bongju ; Lee, Sung-Jae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-4e22204a9cddcb714115e6cdce5411fbc00fced6ba56fd708d7b1f19596b9c143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomechanics</topic><topic>Bones</topic><topic>Dental care</topic><topic>Design</topic><topic>Enamel</topic><topic>Finite element analysis</topic><topic>Finite element method</topic><topic>Inclination</topic><topic>Ligaments</topic><topic>Mathematical models</topic><topic>Orthodontics</topic><topic>Rotation</topic><topic>Stress analysis</topic><topic>Teeth</topic><topic>Thickness</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seo, Jeong-Hee</creatorcontrib><creatorcontrib>Eghan-Acquah, Emmanuel</creatorcontrib><creatorcontrib>Kim, Min-Seok</creatorcontrib><creatorcontrib>Lee, Jeong-Hyeon</creatorcontrib><creatorcontrib>Jeong, Yong-Hoon</creatorcontrib><creatorcontrib>Jung, Tae-Gon</creatorcontrib><creatorcontrib>Hong, Mihee</creatorcontrib><creatorcontrib>Kim, Won-Hyeon</creatorcontrib><creatorcontrib>Kim, Bongju</creatorcontrib><creatorcontrib>Lee, Sung-Jae</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seo, Jeong-Hee</au><au>Eghan-Acquah, Emmanuel</au><au>Kim, Min-Seok</au><au>Lee, Jeong-Hyeon</au><au>Jeong, Yong-Hoon</au><au>Jung, Tae-Gon</au><au>Hong, Mihee</au><au>Kim, Won-Hyeon</au><au>Kim, Bongju</au><au>Lee, Sung-Jae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study</atitle><jtitle>Materials</jtitle><addtitle>Materials (Basel)</addtitle><date>2021-01-09</date><risdate>2021</risdate><volume>14</volume><issue>2</issue><spage>324</spage><pages>324-</pages><issn>1996-1944</issn><eissn>1996-1944</eissn><abstract>Lately, in orthodontic treatments, the use of transparent aligners for the correction of malocclusions has become prominent owing to their intrinsic advantages such as esthetics, comfort, and minimal maintenance. Attempts at improving upon this technology by varying various parameters to investigate the effects on treatments have been carried out by several researchers. Here, we aimed to investigate the biomechanical and clinical effects of aligner thickness on stress distributions in the periodontal ligament and changes in the tooth's center of rotation. Dental finite element models comprising the cortical and cancellous bones, gingiva, teeth, and nonlinear viscoelastic periodontal ligaments were constructed, validated, and used together with aligner finite element models of different aligner thicknesses to achieve the goal of this study. The finite element analyses were conducted to simulate the actual orthodontic aligner treatment process for the correction of malocclusions by generating pre-stresses in the aligner and allowing the aligner stresses to relax to induce tooth movement. The results of the analyses showed that orthodontic treatment in lingual inclination and axial rotation with a 0.75 mm-thick aligner resulted in 6% and 0.03% higher principal stresses in the periodontal ligament than the same treatment using a 0.05 mm-thick aligner, respectively. Again, for both aligner thicknesses, the tooth's center of rotation moved lingually and towards the root direction in lingual inclination, and diagonally from the long axis of the tooth in axial rotation. Taken together, orthodontic treatment for simple malocclusions using transparent aligners of different thicknesses will produce a similar effect on the principal stresses in the periodontal ligament and similar changes in the tooth's center of rotation, as well as sufficient tooth movement. These findings provide orthodontists and researchers clinical and biomechanical evidence about the effect of transparent aligner thickness selection and its effect on orthodontic treatment.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33435457</pmid><doi>10.3390/ma14020324</doi><orcidid>https://orcid.org/0000-0002-0011-6981</orcidid><orcidid>https://orcid.org/0000-0002-4793-9125</orcidid><orcidid>https://orcid.org/0000-0002-6702-1960</orcidid><orcidid>https://orcid.org/0000-0001-6015-1482</orcidid><orcidid>https://orcid.org/0000-0001-7309-5977</orcidid><orcidid>https://orcid.org/0000-0002-9742-7945</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1996-1944 |
ispartof | Materials, 2021-01, Vol.14 (2), p.324 |
issn | 1996-1944 1996-1944 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7826543 |
source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Biomechanics Bones Dental care Design Enamel Finite element analysis Finite element method Inclination Ligaments Mathematical models Orthodontics Rotation Stress analysis Teeth Thickness |
title | Comparative Analysis of Stress in the Periodontal Ligament and Center of Rotation in the Tooth after Orthodontic Treatment Depending on Clear Aligner Thickness-Finite Element Analysis Study |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T12%3A29%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20Analysis%20of%20Stress%20in%20the%20Periodontal%20Ligament%20and%20Center%20of%20Rotation%20in%20the%20Tooth%20after%20Orthodontic%20Treatment%20Depending%20on%20Clear%20Aligner%20Thickness-Finite%20Element%20Analysis%20Study&rft.jtitle=Materials&rft.au=Seo,%20Jeong-Hee&rft.date=2021-01-09&rft.volume=14&rft.issue=2&rft.spage=324&rft.pages=324-&rft.issn=1996-1944&rft.eissn=1996-1944&rft_id=info:doi/10.3390/ma14020324&rft_dat=%3Cproquest_pubme%3E2477819731%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2477819731&rft_id=info:pmid/33435457&rfr_iscdi=true |