Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice
Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from (ATE), enriched in phyto...
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| Veröffentlicht in: | International journal of environmental research and public health 2021-01, Vol.18 (2), p.370 |
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| creator | Lawrence, Marcus M Zwetsloot, Kevin A Arthur, Susan T Sherman, Chase A Huot, Joshua R Badmaev, Vladimir Grace, Mary Lila, Mary Ann Nieman, David C Shanely, R Andrew |
| description | Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from
(ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K-Akt signaling pathway, and the mRNA levels of
,
, and
in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt
and p70S6k
) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of
,
, and
. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice. |
| doi_str_mv | 10.3390/ijerph18020370 |
| format | Article |
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(ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K-Akt signaling pathway, and the mRNA levels of
,
, and
in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt
and p70S6k
) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of
,
, and
. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18020370</identifier><identifier>PMID: 33418916</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>1-Phosphatidylinositol 3-kinase ; Aging ; AKT protein ; Anabolic Agents ; Animals ; Antibodies ; Enzymes ; Laboratory animals ; Male ; Mice ; Mice, Inbred C57BL ; mRNA ; Muscle strength ; Muscle, Skeletal ; Muscles ; Musculoskeletal system ; Myostatin ; Phosphatidylinositol 3-Kinases ; Protein biosynthesis ; Protein synthesis ; Proteins ; Rats ; Sarcopenia ; Sedentary behavior ; Signal transduction ; Skeletal muscle ; Young adults</subject><ispartof>International journal of environmental research and public health, 2021-01, Vol.18 (2), p.370</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-a2ca67082f2ecf41e1bc98c7acf40311b4af933cb5e197250529ea45856d924e3</citedby><cites>FETCH-LOGICAL-c418t-a2ca67082f2ecf41e1bc98c7acf40311b4af933cb5e197250529ea45856d924e3</cites><orcidid>0000-0002-4928-3836 ; 0000-0002-8305-1860 ; 0000-0002-1916-3809</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825148/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825148/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33418916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawrence, Marcus M</creatorcontrib><creatorcontrib>Zwetsloot, Kevin A</creatorcontrib><creatorcontrib>Arthur, Susan T</creatorcontrib><creatorcontrib>Sherman, Chase A</creatorcontrib><creatorcontrib>Huot, Joshua R</creatorcontrib><creatorcontrib>Badmaev, Vladimir</creatorcontrib><creatorcontrib>Grace, Mary</creatorcontrib><creatorcontrib>Lila, Mary Ann</creatorcontrib><creatorcontrib>Nieman, David C</creatorcontrib><creatorcontrib>Shanely, R Andrew</creatorcontrib><title>Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from
(ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K-Akt signaling pathway, and the mRNA levels of
,
, and
in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt
and p70S6k
) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of
,
, and
. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Aging</subject><subject>AKT protein</subject><subject>Anabolic Agents</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Enzymes</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>mRNA</subject><subject>Muscle strength</subject><subject>Muscle, Skeletal</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myostatin</subject><subject>Phosphatidylinositol 3-Kinases</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Rats</subject><subject>Sarcopenia</subject><subject>Sedentary behavior</subject><subject>Signal transduction</subject><subject>Skeletal muscle</subject><subject>Young adults</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkUtLxDAUhYMovrcuJeDGzWheTZuNMIxP8AXqwlVI09uZjJ1mTFph_r3RUVFXNzf5cjiHg9AeJUecK3LsphDmE1oQRnhOVtAmlZIMhCR09dd5A23FOCWEF0KqdbTBuaCFonITPd9PFp0HWy1iB8G7KuJTj299hy_NG-Bha0rfOIvP6hpsF7Fr8cMLNNCZBt_00TbweQUVtJ0JCzwcu3aMb5yFHbRWmybC7tfcRk_nZ4-jy8H13cXVaHg9sMlDNzDMGpmTgtUMbC0o0NKqwuYmLYRTWgpTK85tmQFVOctIxhQYkRWZrBQTwLfRyVJ33pczqGwyEkyj58HNkiHtjdN_X1o30WP_pvOCZVQUSeDwSyD41x5ip2cuWmga04Lvo2Yil5mkiU7owT906vvQpnhLSnGS0UQdLSkbfIwB6h8zlOiP1vTf1tKH_d8RfvDvmvg71HiT_Q</recordid><startdate>20210106</startdate><enddate>20210106</enddate><creator>Lawrence, Marcus M</creator><creator>Zwetsloot, Kevin A</creator><creator>Arthur, Susan T</creator><creator>Sherman, Chase A</creator><creator>Huot, Joshua R</creator><creator>Badmaev, Vladimir</creator><creator>Grace, Mary</creator><creator>Lila, Mary Ann</creator><creator>Nieman, David C</creator><creator>Shanely, R Andrew</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4928-3836</orcidid><orcidid>https://orcid.org/0000-0002-8305-1860</orcidid><orcidid>https://orcid.org/0000-0002-1916-3809</orcidid></search><sort><creationdate>20210106</creationdate><title>Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice</title><author>Lawrence, Marcus M ; 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Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from
(ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K-Akt signaling pathway, and the mRNA levels of
,
, and
in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt
and p70S6k
) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of
,
, and
. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33418916</pmid><doi>10.3390/ijerph18020370</doi><orcidid>https://orcid.org/0000-0002-4928-3836</orcidid><orcidid>https://orcid.org/0000-0002-8305-1860</orcidid><orcidid>https://orcid.org/0000-0002-1916-3809</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of environmental research and public health, 2021-01, Vol.18 (2), p.370 |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; PubMed Central Open Access |
| subjects | 1-Phosphatidylinositol 3-kinase Aging AKT protein Anabolic Agents Animals Antibodies Enzymes Laboratory animals Male Mice Mice, Inbred C57BL mRNA Muscle strength Muscle, Skeletal Muscles Musculoskeletal system Myostatin Phosphatidylinositol 3-Kinases Protein biosynthesis Protein synthesis Proteins Rats Sarcopenia Sedentary behavior Signal transduction Skeletal muscle Young adults |
| title | Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice |
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