Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish
Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2021-01, Vol.25 (2), p.1048-1059 |
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| creator | Khan, Alam Fahad, Talukdar Mohammad Akther, Tanjima Zaman, Tanjeena Hasan, Md Faruk Islam Khan, Md Rafiqual Islam, Mohammad Saiful Kishi, Shuji |
| description | Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence‐associated β‐galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up‐regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double‐transgenic zebrafish line, harbouring EGFP‐tagged LC3‐II and mCherry‐tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome‐mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals. |
| doi_str_mv | 10.1111/jcmm.16171 |
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In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence‐associated β‐galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up‐regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double‐transgenic zebrafish line, harbouring EGFP‐tagged LC3‐II and mCherry‐tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome‐mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.16171</identifier><identifier>PMID: 33277797</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Aging ; Agricultural practices ; Apoptosis ; Autophagy ; Carbofuran ; Cell cycle ; Danio rerio ; Deoxyribonucleic acid ; DNA ; Embryos ; Enzymes ; Experiments ; Fish ; Hippocampus ; life span ; Lysosomes ; Morphology ; Mutants ; Mutation ; Nervous system ; Original ; Oxidative stress ; Pesticides ; Phagocytosis ; Phenotypes ; Plasmids ; Potassium ; RNA polymerase ; Senescence ; spinster homolog 1 ; Toxicity ; Yolk ; Zebrafish ; β-Galactosidase</subject><ispartof>Journal of cellular and molecular medicine, 2021-01, Vol.25 (2), p.1048-1059</ispartof><rights>2020 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd</rights><rights>2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4761-36d3520007402a177193bfe0bce0fc67d678576c72b467ce9a9661d27412659a3</citedby><cites>FETCH-LOGICAL-c4761-36d3520007402a177193bfe0bce0fc67d678576c72b467ce9a9661d27412659a3</cites><orcidid>0000-0002-2482-7403</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812278/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812278/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33277797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Alam</creatorcontrib><creatorcontrib>Fahad, Talukdar Mohammad</creatorcontrib><creatorcontrib>Akther, Tanjima</creatorcontrib><creatorcontrib>Zaman, Tanjeena</creatorcontrib><creatorcontrib>Hasan, Md Faruk</creatorcontrib><creatorcontrib>Islam Khan, Md Rafiqual</creatorcontrib><creatorcontrib>Islam, Mohammad Saiful</creatorcontrib><creatorcontrib>Kishi, Shuji</creatorcontrib><title>Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence‐associated β‐galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up‐regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double‐transgenic zebrafish line, harbouring EGFP‐tagged LC3‐II and mCherry‐tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome‐mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.</description><subject>Aging</subject><subject>Agricultural practices</subject><subject>Apoptosis</subject><subject>Autophagy</subject><subject>Carbofuran</subject><subject>Cell cycle</subject><subject>Danio rerio</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Embryos</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Fish</subject><subject>Hippocampus</subject><subject>life span</subject><subject>Lysosomes</subject><subject>Morphology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nervous system</subject><subject>Original</subject><subject>Oxidative stress</subject><subject>Pesticides</subject><subject>Phagocytosis</subject><subject>Phenotypes</subject><subject>Plasmids</subject><subject>Potassium</subject><subject>RNA 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accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish</title><author>Khan, Alam ; Fahad, Talukdar Mohammad ; Akther, Tanjima ; Zaman, Tanjeena ; Hasan, Md Faruk ; Islam Khan, Md Rafiqual ; Islam, Mohammad Saiful ; Kishi, Shuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4761-36d3520007402a177193bfe0bce0fc67d678576c72b467ce9a9661d27412659a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aging</topic><topic>Agricultural practices</topic><topic>Apoptosis</topic><topic>Autophagy</topic><topic>Carbofuran</topic><topic>Cell cycle</topic><topic>Danio rerio</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Embryos</topic><topic>Enzymes</topic><topic>Experiments</topic><topic>Fish</topic><topic>Hippocampus</topic><topic>life 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Shuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2021-01</date><risdate>2021</risdate><volume>25</volume><issue>2</issue><spage>1048</spage><epage>1059</epage><pages>1048-1059</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence‐associated β‐galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up‐regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double‐transgenic zebrafish line, harbouring EGFP‐tagged LC3‐II and mCherry‐tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome‐mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>33277797</pmid><doi>10.1111/jcmm.16171</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2482-7403</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Journals; DOAJ Directory of Open Access Journals; Wiley-Blackwell Open Access Titles; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Aging Agricultural practices Apoptosis Autophagy Carbofuran Cell cycle Danio rerio Deoxyribonucleic acid DNA Embryos Enzymes Experiments Fish Hippocampus life span Lysosomes Morphology Mutants Mutation Nervous system Original Oxidative stress Pesticides Phagocytosis Phenotypes Plasmids Potassium RNA polymerase Senescence spinster homolog 1 Toxicity Yolk Zebrafish β-Galactosidase |
| title | Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish |
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