Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis
Background Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of...
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| Veröffentlicht in: | Thoracic cancer 2021-01, Vol.12 (2), p.251-258 |
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| creator | Tone, Mari Inomata, Minoru Awano, Nobuyasu Kuse, Naoyuki Takada, Kohei Minami, Jonsu Muto, Yutaka Fujimoto, Kazushi Kumasaka, Toshio Izumo, Takehiro |
| description | Background
Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of these samples for next‐generation sequencing (NGS) analysis remains unclear. This study aimed to compare the adequacy between TBLC and EBUS‐TBNA samples for NGS analysis.
Methods
This retrospective cohort study included patients whose lung samples were collected via TBLC or EBUS‐TBNA and analyzed using NGS. Out of 46 genes, the number of genes in TBNA and TBLC samples that could not be assessed via NGS analysis was mainly evaluated.
Results
A total of 37 patients were included and classified into two groups (TBLC group, n = 18 and TBNA group, n = 19). The mean number of genes that could not be evaluated via NGS analysis was significantly lower in the TBLC group than in the TBNA group (0.9 vs. 10.3, P = 0.024). The median total area of tumor cells in TBLC samples was significantly greater than that in TBNA samples (6.3 [1.6–4.2] vs. 2.6 [0.2–17.3] mm2, P |
| doi_str_mv | 10.1111/1759-7714.13770 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7812063</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2466772709</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5330-59830d6eade2cd2c8c56c21aec02fa0de83f388a9e48b87bcd061931d21084d13</originalsourceid><addsrcrecordid>eNqFks1u1DAUhSMEolXbNTtkiQ2baf2TxM4GqRpBQarEpqwtx76ZunLsYCeU7PoIfSIepk-Cw3SG0g3e2PL97tE9uqco3hB8SvI5I7xqVpyT8pQwzvGL4nD_83L_xvVBcZLSDc6HiQbT6nVxwBjlmNXNYfFrHfpBRZuCR6FDysD3SekZtTDeAng0RuVTG4PX11Y55Ca_QTrOobVhSDNKqh8cJKS8QeBN-EtOLremMHnzcHe_mawB81zMAxgHSKXBRjXaPMFOrgsxV3-OSyt42FXzbOC1zSMor9ycbDouXnXKJTh5vI-Kb58-Xq0_ry6_XnxZn1-udMUYXlWNYNjUkO1RbagWuqo1JQo0pp3CBgTrmBCqgVK0grfa4Jo0jBhKsCgNYUfFh63uMLU9GA0-e3FyiLZXcZZBWflvxdtruQk_JBeE4pplgfePAjFkF2mUvU0anFMewpQkLeua87yVJqPvnqE3YYrZ8EJxwfIOaZmpsy2lY0gpQrcfhmC5xEMuAZBLGOSfeOSOt0897PldGDJQbYFb62D-n568Wp9vhX8D79zONA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2478390224</pqid></control><display><type>article</type><title>Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis</title><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Tone, Mari ; Inomata, Minoru ; Awano, Nobuyasu ; Kuse, Naoyuki ; Takada, Kohei ; Minami, Jonsu ; Muto, Yutaka ; Fujimoto, Kazushi ; Kumasaka, Toshio ; Izumo, Takehiro</creator><creatorcontrib>Tone, Mari ; Inomata, Minoru ; Awano, Nobuyasu ; Kuse, Naoyuki ; Takada, Kohei ; Minami, Jonsu ; Muto, Yutaka ; Fujimoto, Kazushi ; Kumasaka, Toshio ; Izumo, Takehiro</creatorcontrib><description>Background
Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of these samples for next‐generation sequencing (NGS) analysis remains unclear. This study aimed to compare the adequacy between TBLC and EBUS‐TBNA samples for NGS analysis.
Methods
This retrospective cohort study included patients whose lung samples were collected via TBLC or EBUS‐TBNA and analyzed using NGS. Out of 46 genes, the number of genes in TBNA and TBLC samples that could not be assessed via NGS analysis was mainly evaluated.
Results
A total of 37 patients were included and classified into two groups (TBLC group, n = 18 and TBNA group, n = 19). The mean number of genes that could not be evaluated via NGS analysis was significantly lower in the TBLC group than in the TBNA group (0.9 vs. 10.3, P = 0.024). The median total area of tumor cells in TBLC samples was significantly greater than that in TBNA samples (6.3 [1.6–4.2] vs. 2.6 [0.2–17.3] mm2, P < 0.01). In the TBNA group, there were two fully inadequate samples for NGS analysis with a high degree of cell crush or low tumor content, while there was no fully inadequate sample in the TBLC group.
Conclusions
TBLC is more effective in obtaining adequate samples for NGS analysis than EBUS‐TBNA. TBLC should be performed to obtain adequate samples for NGS analysis in lung cancer patients with target lesions in lung fields, even if they have lymphadenopathy.
Key points
Significant findings of the study
The mean number of genes that could not be evaluated was significantly lower in TBLC samples than in EBUS‐TBNA samples (0.9 vs. 10.3, P = 0.024). TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.
What this study adds
To obtain samples for NGS analysis, the use of TBLC should be aggressively considered in lung‐cancer patients with target lesions located in lung fields, even if they have lymphadenopathy.
The adequacy for next generation sequencing (NGS) in transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) samples remains unclear. We found the mean number of genes which could not be assessed via NGS was significantly lower in TBLC samples than EBUS‐TBNA samples. TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.</description><identifier>ISSN: 1759-7706</identifier><identifier>EISSN: 1759-7714</identifier><identifier>DOI: 10.1111/1759-7714.13770</identifier><identifier>PMID: 33270369</identifier><language>eng</language><publisher>Melbourne: John Wiley & Sons Australia, Ltd</publisher><subject>Biopsy ; Bronchoscopy ; Cancer therapies ; Catheters ; Clinical medicine ; cryobiopsy ; endobronchial ultrasound‐guided transbronchial needle aspiration ; Genes ; Lung cancer ; Lymphatic system ; Mutation ; next‐generation sequencing ; Original ; Ultrasonic imaging</subject><ispartof>Thoracic cancer, 2021-01, Vol.12 (2), p.251-258</ispartof><rights>2020 The Authors. published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd</rights><rights>2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5330-59830d6eade2cd2c8c56c21aec02fa0de83f388a9e48b87bcd061931d21084d13</citedby><cites>FETCH-LOGICAL-c5330-59830d6eade2cd2c8c56c21aec02fa0de83f388a9e48b87bcd061931d21084d13</cites><orcidid>0000-0002-8230-0757</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812063/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812063/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33270369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tone, Mari</creatorcontrib><creatorcontrib>Inomata, Minoru</creatorcontrib><creatorcontrib>Awano, Nobuyasu</creatorcontrib><creatorcontrib>Kuse, Naoyuki</creatorcontrib><creatorcontrib>Takada, Kohei</creatorcontrib><creatorcontrib>Minami, Jonsu</creatorcontrib><creatorcontrib>Muto, Yutaka</creatorcontrib><creatorcontrib>Fujimoto, Kazushi</creatorcontrib><creatorcontrib>Kumasaka, Toshio</creatorcontrib><creatorcontrib>Izumo, Takehiro</creatorcontrib><title>Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis</title><title>Thoracic cancer</title><addtitle>Thorac Cancer</addtitle><description>Background
Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of these samples for next‐generation sequencing (NGS) analysis remains unclear. This study aimed to compare the adequacy between TBLC and EBUS‐TBNA samples for NGS analysis.
Methods
This retrospective cohort study included patients whose lung samples were collected via TBLC or EBUS‐TBNA and analyzed using NGS. Out of 46 genes, the number of genes in TBNA and TBLC samples that could not be assessed via NGS analysis was mainly evaluated.
Results
A total of 37 patients were included and classified into two groups (TBLC group, n = 18 and TBNA group, n = 19). The mean number of genes that could not be evaluated via NGS analysis was significantly lower in the TBLC group than in the TBNA group (0.9 vs. 10.3, P = 0.024). The median total area of tumor cells in TBLC samples was significantly greater than that in TBNA samples (6.3 [1.6–4.2] vs. 2.6 [0.2–17.3] mm2, P < 0.01). In the TBNA group, there were two fully inadequate samples for NGS analysis with a high degree of cell crush or low tumor content, while there was no fully inadequate sample in the TBLC group.
Conclusions
TBLC is more effective in obtaining adequate samples for NGS analysis than EBUS‐TBNA. TBLC should be performed to obtain adequate samples for NGS analysis in lung cancer patients with target lesions in lung fields, even if they have lymphadenopathy.
Key points
Significant findings of the study
The mean number of genes that could not be evaluated was significantly lower in TBLC samples than in EBUS‐TBNA samples (0.9 vs. 10.3, P = 0.024). TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.
What this study adds
To obtain samples for NGS analysis, the use of TBLC should be aggressively considered in lung‐cancer patients with target lesions located in lung fields, even if they have lymphadenopathy.
The adequacy for next generation sequencing (NGS) in transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) samples remains unclear. We found the mean number of genes which could not be assessed via NGS was significantly lower in TBLC samples than EBUS‐TBNA samples. TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.</description><subject>Biopsy</subject><subject>Bronchoscopy</subject><subject>Cancer therapies</subject><subject>Catheters</subject><subject>Clinical medicine</subject><subject>cryobiopsy</subject><subject>endobronchial ultrasound‐guided transbronchial needle aspiration</subject><subject>Genes</subject><subject>Lung cancer</subject><subject>Lymphatic system</subject><subject>Mutation</subject><subject>next‐generation sequencing</subject><subject>Original</subject><subject>Ultrasonic imaging</subject><issn>1759-7706</issn><issn>1759-7714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNqFks1u1DAUhSMEolXbNTtkiQ2baf2TxM4GqRpBQarEpqwtx76ZunLsYCeU7PoIfSIepk-Cw3SG0g3e2PL97tE9uqco3hB8SvI5I7xqVpyT8pQwzvGL4nD_83L_xvVBcZLSDc6HiQbT6nVxwBjlmNXNYfFrHfpBRZuCR6FDysD3SekZtTDeAng0RuVTG4PX11Y55Ca_QTrOobVhSDNKqh8cJKS8QeBN-EtOLremMHnzcHe_mawB81zMAxgHSKXBRjXaPMFOrgsxV3-OSyt42FXzbOC1zSMor9ycbDouXnXKJTh5vI-Kb58-Xq0_ry6_XnxZn1-udMUYXlWNYNjUkO1RbagWuqo1JQo0pp3CBgTrmBCqgVK0grfa4Jo0jBhKsCgNYUfFh63uMLU9GA0-e3FyiLZXcZZBWflvxdtruQk_JBeE4pplgfePAjFkF2mUvU0anFMewpQkLeua87yVJqPvnqE3YYrZ8EJxwfIOaZmpsy2lY0gpQrcfhmC5xEMuAZBLGOSfeOSOt0897PldGDJQbYFb62D-n568Wp9vhX8D79zONA</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Tone, Mari</creator><creator>Inomata, Minoru</creator><creator>Awano, Nobuyasu</creator><creator>Kuse, Naoyuki</creator><creator>Takada, Kohei</creator><creator>Minami, Jonsu</creator><creator>Muto, Yutaka</creator><creator>Fujimoto, Kazushi</creator><creator>Kumasaka, Toshio</creator><creator>Izumo, Takehiro</creator><general>John Wiley & Sons Australia, Ltd</general><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8230-0757</orcidid></search><sort><creationdate>202101</creationdate><title>Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis</title><author>Tone, Mari ; Inomata, Minoru ; Awano, Nobuyasu ; Kuse, Naoyuki ; Takada, Kohei ; Minami, Jonsu ; Muto, Yutaka ; Fujimoto, Kazushi ; Kumasaka, Toshio ; Izumo, Takehiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5330-59830d6eade2cd2c8c56c21aec02fa0de83f388a9e48b87bcd061931d21084d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biopsy</topic><topic>Bronchoscopy</topic><topic>Cancer therapies</topic><topic>Catheters</topic><topic>Clinical medicine</topic><topic>cryobiopsy</topic><topic>endobronchial ultrasound‐guided transbronchial needle aspiration</topic><topic>Genes</topic><topic>Lung cancer</topic><topic>Lymphatic system</topic><topic>Mutation</topic><topic>next‐generation sequencing</topic><topic>Original</topic><topic>Ultrasonic imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tone, Mari</creatorcontrib><creatorcontrib>Inomata, Minoru</creatorcontrib><creatorcontrib>Awano, Nobuyasu</creatorcontrib><creatorcontrib>Kuse, Naoyuki</creatorcontrib><creatorcontrib>Takada, Kohei</creatorcontrib><creatorcontrib>Minami, Jonsu</creatorcontrib><creatorcontrib>Muto, Yutaka</creatorcontrib><creatorcontrib>Fujimoto, Kazushi</creatorcontrib><creatorcontrib>Kumasaka, Toshio</creatorcontrib><creatorcontrib>Izumo, Takehiro</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Thoracic cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tone, Mari</au><au>Inomata, Minoru</au><au>Awano, Nobuyasu</au><au>Kuse, Naoyuki</au><au>Takada, Kohei</au><au>Minami, Jonsu</au><au>Muto, Yutaka</au><au>Fujimoto, Kazushi</au><au>Kumasaka, Toshio</au><au>Izumo, Takehiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis</atitle><jtitle>Thoracic cancer</jtitle><addtitle>Thorac Cancer</addtitle><date>2021-01</date><risdate>2021</risdate><volume>12</volume><issue>2</issue><spage>251</spage><epage>258</epage><pages>251-258</pages><issn>1759-7706</issn><eissn>1759-7714</eissn><abstract>Background
Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of these samples for next‐generation sequencing (NGS) analysis remains unclear. This study aimed to compare the adequacy between TBLC and EBUS‐TBNA samples for NGS analysis.
Methods
This retrospective cohort study included patients whose lung samples were collected via TBLC or EBUS‐TBNA and analyzed using NGS. Out of 46 genes, the number of genes in TBNA and TBLC samples that could not be assessed via NGS analysis was mainly evaluated.
Results
A total of 37 patients were included and classified into two groups (TBLC group, n = 18 and TBNA group, n = 19). The mean number of genes that could not be evaluated via NGS analysis was significantly lower in the TBLC group than in the TBNA group (0.9 vs. 10.3, P = 0.024). The median total area of tumor cells in TBLC samples was significantly greater than that in TBNA samples (6.3 [1.6–4.2] vs. 2.6 [0.2–17.3] mm2, P < 0.01). In the TBNA group, there were two fully inadequate samples for NGS analysis with a high degree of cell crush or low tumor content, while there was no fully inadequate sample in the TBLC group.
Conclusions
TBLC is more effective in obtaining adequate samples for NGS analysis than EBUS‐TBNA. TBLC should be performed to obtain adequate samples for NGS analysis in lung cancer patients with target lesions in lung fields, even if they have lymphadenopathy.
Key points
Significant findings of the study
The mean number of genes that could not be evaluated was significantly lower in TBLC samples than in EBUS‐TBNA samples (0.9 vs. 10.3, P = 0.024). TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.
What this study adds
To obtain samples for NGS analysis, the use of TBLC should be aggressively considered in lung‐cancer patients with target lesions located in lung fields, even if they have lymphadenopathy.
The adequacy for next generation sequencing (NGS) in transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) samples remains unclear. We found the mean number of genes which could not be assessed via NGS was significantly lower in TBLC samples than EBUS‐TBNA samples. TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.</abstract><cop>Melbourne</cop><pub>John Wiley & Sons Australia, Ltd</pub><pmid>33270369</pmid><doi>10.1111/1759-7714.13770</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8230-0757</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biopsy Bronchoscopy Cancer therapies Catheters Clinical medicine cryobiopsy endobronchial ultrasound‐guided transbronchial needle aspiration Genes Lung cancer Lymphatic system Mutation next‐generation sequencing Original Ultrasonic imaging |
| title | Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound‐guided transbronchial needle aspiration samples for next‐generation sequencing analysis |
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