Time-resolved cryoEM using Spotiton
We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified...
Gespeichert in:
| Veröffentlicht in: | Nature methods 2020-08, Vol.17 (9), p.897-900 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 900 |
|---|---|
| container_issue | 9 |
| container_start_page | 897 |
| container_title | Nature methods |
| container_volume | 17 |
| creator | Dandey, Venkata P. Budell, William C. Wei, Hui Bobe, Daija Maruthi, Kashyap Kopylov, Mykhailo Eng, Edward T. Kahn, Peter A. Hinshaw, Jenny E. Kundu, Nidhi Nimigean, Crina M. Fan, Chen Sukomon, Nattakan Darst, Seth A. Saecker, Ruth M. Chen, James Malone, Brandon Potter, Clinton S. Carragher, Bridget |
| description | We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest. |
| doi_str_mv | 10.1038/s41592-020-0925-6 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7799389</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_7799389</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_77993893</originalsourceid><addsrcrecordid>eNqljL0KwjAYAIMo1r8HcCs4R78kjUkWF1FcnHQP1cYaaZuStIW-vYsOzk53cHAILQmsCTC5CQnhimKggEFRjrcDNCE8kVgQ4MOvgyIRmobwAmAsoXyMIkaFkJKxCVpdbWmwN8EVncniu-_d4Ry3wVZ5fKldYxtXzdHokRbBLD6cod3xcN2fcN3eSpPdTdX4tNC1t2Xqe-1Sq39LZZ86d50WQikmFft78AZ4rU3u</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Time-resolved cryoEM using Spotiton</title><source>SpringerLink Journals</source><source>Nature</source><creator>Dandey, Venkata P. ; Budell, William C. ; Wei, Hui ; Bobe, Daija ; Maruthi, Kashyap ; Kopylov, Mykhailo ; Eng, Edward T. ; Kahn, Peter A. ; Hinshaw, Jenny E. ; Kundu, Nidhi ; Nimigean, Crina M. ; Fan, Chen ; Sukomon, Nattakan ; Darst, Seth A. ; Saecker, Ruth M. ; Chen, James ; Malone, Brandon ; Potter, Clinton S. ; Carragher, Bridget</creator><creatorcontrib>Dandey, Venkata P. ; Budell, William C. ; Wei, Hui ; Bobe, Daija ; Maruthi, Kashyap ; Kopylov, Mykhailo ; Eng, Edward T. ; Kahn, Peter A. ; Hinshaw, Jenny E. ; Kundu, Nidhi ; Nimigean, Crina M. ; Fan, Chen ; Sukomon, Nattakan ; Darst, Seth A. ; Saecker, Ruth M. ; Chen, James ; Malone, Brandon ; Potter, Clinton S. ; Carragher, Bridget</creatorcontrib><description>We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/s41592-020-0925-6</identifier><identifier>PMID: 32778833</identifier><language>eng</language><ispartof>Nature methods, 2020-08, Vol.17 (9), p.897-900</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids></links><search><creatorcontrib>Dandey, Venkata P.</creatorcontrib><creatorcontrib>Budell, William C.</creatorcontrib><creatorcontrib>Wei, Hui</creatorcontrib><creatorcontrib>Bobe, Daija</creatorcontrib><creatorcontrib>Maruthi, Kashyap</creatorcontrib><creatorcontrib>Kopylov, Mykhailo</creatorcontrib><creatorcontrib>Eng, Edward T.</creatorcontrib><creatorcontrib>Kahn, Peter A.</creatorcontrib><creatorcontrib>Hinshaw, Jenny E.</creatorcontrib><creatorcontrib>Kundu, Nidhi</creatorcontrib><creatorcontrib>Nimigean, Crina M.</creatorcontrib><creatorcontrib>Fan, Chen</creatorcontrib><creatorcontrib>Sukomon, Nattakan</creatorcontrib><creatorcontrib>Darst, Seth A.</creatorcontrib><creatorcontrib>Saecker, Ruth M.</creatorcontrib><creatorcontrib>Chen, James</creatorcontrib><creatorcontrib>Malone, Brandon</creatorcontrib><creatorcontrib>Potter, Clinton S.</creatorcontrib><creatorcontrib>Carragher, Bridget</creatorcontrib><title>Time-resolved cryoEM using Spotiton</title><title>Nature methods</title><description>We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.</description><issn>1548-7091</issn><issn>1548-7105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqljL0KwjAYAIMo1r8HcCs4R78kjUkWF1FcnHQP1cYaaZuStIW-vYsOzk53cHAILQmsCTC5CQnhimKggEFRjrcDNCE8kVgQ4MOvgyIRmobwAmAsoXyMIkaFkJKxCVpdbWmwN8EVncniu-_d4Ry3wVZ5fKldYxtXzdHokRbBLD6cod3xcN2fcN3eSpPdTdX4tNC1t2Xqe-1Sq39LZZ86d50WQikmFft78AZ4rU3u</recordid><startdate>20200810</startdate><enddate>20200810</enddate><creator>Dandey, Venkata P.</creator><creator>Budell, William C.</creator><creator>Wei, Hui</creator><creator>Bobe, Daija</creator><creator>Maruthi, Kashyap</creator><creator>Kopylov, Mykhailo</creator><creator>Eng, Edward T.</creator><creator>Kahn, Peter A.</creator><creator>Hinshaw, Jenny E.</creator><creator>Kundu, Nidhi</creator><creator>Nimigean, Crina M.</creator><creator>Fan, Chen</creator><creator>Sukomon, Nattakan</creator><creator>Darst, Seth A.</creator><creator>Saecker, Ruth M.</creator><creator>Chen, James</creator><creator>Malone, Brandon</creator><creator>Potter, Clinton S.</creator><creator>Carragher, Bridget</creator><scope>5PM</scope></search><sort><creationdate>20200810</creationdate><title>Time-resolved cryoEM using Spotiton</title><author>Dandey, Venkata P. ; Budell, William C. ; Wei, Hui ; Bobe, Daija ; Maruthi, Kashyap ; Kopylov, Mykhailo ; Eng, Edward T. ; Kahn, Peter A. ; Hinshaw, Jenny E. ; Kundu, Nidhi ; Nimigean, Crina M. ; Fan, Chen ; Sukomon, Nattakan ; Darst, Seth A. ; Saecker, Ruth M. ; Chen, James ; Malone, Brandon ; Potter, Clinton S. ; Carragher, Bridget</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_77993893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dandey, Venkata P.</creatorcontrib><creatorcontrib>Budell, William C.</creatorcontrib><creatorcontrib>Wei, Hui</creatorcontrib><creatorcontrib>Bobe, Daija</creatorcontrib><creatorcontrib>Maruthi, Kashyap</creatorcontrib><creatorcontrib>Kopylov, Mykhailo</creatorcontrib><creatorcontrib>Eng, Edward T.</creatorcontrib><creatorcontrib>Kahn, Peter A.</creatorcontrib><creatorcontrib>Hinshaw, Jenny E.</creatorcontrib><creatorcontrib>Kundu, Nidhi</creatorcontrib><creatorcontrib>Nimigean, Crina M.</creatorcontrib><creatorcontrib>Fan, Chen</creatorcontrib><creatorcontrib>Sukomon, Nattakan</creatorcontrib><creatorcontrib>Darst, Seth A.</creatorcontrib><creatorcontrib>Saecker, Ruth M.</creatorcontrib><creatorcontrib>Chen, James</creatorcontrib><creatorcontrib>Malone, Brandon</creatorcontrib><creatorcontrib>Potter, Clinton S.</creatorcontrib><creatorcontrib>Carragher, Bridget</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dandey, Venkata P.</au><au>Budell, William C.</au><au>Wei, Hui</au><au>Bobe, Daija</au><au>Maruthi, Kashyap</au><au>Kopylov, Mykhailo</au><au>Eng, Edward T.</au><au>Kahn, Peter A.</au><au>Hinshaw, Jenny E.</au><au>Kundu, Nidhi</au><au>Nimigean, Crina M.</au><au>Fan, Chen</au><au>Sukomon, Nattakan</au><au>Darst, Seth A.</au><au>Saecker, Ruth M.</au><au>Chen, James</au><au>Malone, Brandon</au><au>Potter, Clinton S.</au><au>Carragher, Bridget</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-resolved cryoEM using Spotiton</atitle><jtitle>Nature methods</jtitle><date>2020-08-10</date><risdate>2020</risdate><volume>17</volume><issue>9</issue><spage>897</spage><epage>900</epage><pages>897-900</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified in liquid ethane, ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.</abstract><pmid>32778833</pmid><doi>10.1038/s41592-020-0925-6</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1548-7091 |
| ispartof | Nature methods, 2020-08, Vol.17 (9), p.897-900 |
| issn | 1548-7091 1548-7105 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7799389 |
| source | SpringerLink Journals; Nature |
| title | Time-resolved cryoEM using Spotiton |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T02%3A28%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Time-resolved%20cryoEM%20using%20Spotiton&rft.jtitle=Nature%20methods&rft.au=Dandey,%20Venkata%20P.&rft.date=2020-08-10&rft.volume=17&rft.issue=9&rft.spage=897&rft.epage=900&rft.pages=897-900&rft.issn=1548-7091&rft.eissn=1548-7105&rft_id=info:doi/10.1038/s41592-020-0925-6&rft_dat=%3Cpubmedcentral%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_7799389%3C/pubmedcentral%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/32778833&rfr_iscdi=true |