A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A
An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemical method, and aptamer technologies. The assembly of the aptasensor was based on the activation of silani...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2020-09, Vol.187 (9), p.535-535, Article 535 |
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| container_title | Mikrochimica acta (1966) |
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| creator | El-Moghazy, Ahmed Y. Amaly, Noha Istamboulie, Georges Nitin, Nitin Sun, Gang |
| description | An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemical method, and aptamer technologies. The assembly of the aptasensor was based on the activation of silanized cellulose nanofibrous membranes as a supporting matrix for methylene blue (MB) redox probe-labeled aptamer tethering. Cellulose nanofibrous membranes were regenerated by deacetylating electrospun cellulose acetate nanofibrous membranes with deacetylation efficacy of 97%, followed by silanization of the nanofiber surfaces by using (3-aminopropyl)triethoxysilane (APTES). A replacement of conventionally casted membranes by the nanofibrous membranes increased the active surface area on the working electrode of a screen-printed three-electrode sensor by more than two times, consequently enhancing the fabricated aptasensor performance. The developed aptasensor demonstrated high sensitivity and specificity toward OTA in a range 0.002–2 ng mL
−1
, with a detection limit of 0.81 pg mL
−1
. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over long storage time.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-020-04509-y |
| format | Article |
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−1
, with a detection limit of 0.81 pg mL
−1
. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over long storage time.
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−1
, with a detection limit of 0.81 pg mL
−1
. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over long storage time.
Graphical abstract</description><subject>Aminopropyltriethoxysilane</subject><subject>Analytical Chemistry</subject><subject>Cellulose</subject><subject>Cellulose acetate</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical Sciences</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Coffee</subject><subject>Electric properties</subject><subject>Electrodes</subject><subject>Membranes</subject><subject>Methylene blue</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanofibers</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Pretreatment</subject><subject>Sensors</subject><subject>Tethering</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9ks1u1DAUhS0EokPhBVigSKxYuFz_xE42SKOKUqSR2MDachx7xlXGDnZSddjw6vWQ0lKEUBaO7e-ca18fhF4TOCMA8n0GEMAxUMDAa2jx4QlaEc4ErkGyp2gFQAVmQtIT9CLnKwAiBeXP0QmjjQTWyhX6ua6y3wY94BgqO1gzpWh2du-NHio9TjrbkGOquvLTV4XJftDB_ygTY4dhHmK2VdAhOt_ZlCtX2KRH31dj9GHC0eG5EL2dirUv-uiqUiDpKd74UK1fomdOD9m-uhtP0beLj1_PL_Hmy6fP5-sNNrWsJ2yJM5wKabjrSdMIoXsghBnZMN46pp2sBWUaCO2ahnSWS0aZ7eu-Y8b1lrNT9GHxHedub3tjw5T0oMbk9zodVNRePd4Jfqe28VpJ2UBTHw3eLQa7v2SX6406rpV-csKIuCaFfXtXLMXvs82TuopzKk3OinLWsnITgAdqqwerfHCxFDZ7n41aS1JDLSRvC3X2D6p8_fGRYrDOl_VHAroITIo5J-vuT0tAHXOjltyokhv1KzfqUERv_mzPveR3UArAFiCXrbC16eFK_7G9BcuIzyo</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>El-Moghazy, Ahmed Y.</creator><creator>Amaly, Noha</creator><creator>Istamboulie, Georges</creator><creator>Nitin, Nitin</creator><creator>Sun, Gang</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5743-7305</orcidid><orcidid>https://orcid.org/0000-0002-1083-4282</orcidid></search><sort><creationdate>20200901</creationdate><title>A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A</title><author>El-Moghazy, Ahmed Y. ; Amaly, Noha ; Istamboulie, Georges ; Nitin, Nitin ; Sun, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-e1fc4267c4fd18866ad0113c78349f3af75623a012b881be47323ed5db3cfde43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aminopropyltriethoxysilane</topic><topic>Analytical Chemistry</topic><topic>Cellulose</topic><topic>Cellulose acetate</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical Sciences</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Coffee</topic><topic>Electric properties</topic><topic>Electrodes</topic><topic>Membranes</topic><topic>Methylene blue</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanofibers</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Pretreatment</topic><topic>Sensors</topic><topic>Tethering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Moghazy, Ahmed Y.</creatorcontrib><creatorcontrib>Amaly, Noha</creatorcontrib><creatorcontrib>Istamboulie, Georges</creatorcontrib><creatorcontrib>Nitin, Nitin</creatorcontrib><creatorcontrib>Sun, Gang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Moghazy, Ahmed Y.</au><au>Amaly, Noha</au><au>Istamboulie, Georges</au><au>Nitin, Nitin</au><au>Sun, Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>187</volume><issue>9</issue><spage>535</spage><epage>535</epage><pages>535-535</pages><artnum>535</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemical method, and aptamer technologies. The assembly of the aptasensor was based on the activation of silanized cellulose nanofibrous membranes as a supporting matrix for methylene blue (MB) redox probe-labeled aptamer tethering. Cellulose nanofibrous membranes were regenerated by deacetylating electrospun cellulose acetate nanofibrous membranes with deacetylation efficacy of 97%, followed by silanization of the nanofiber surfaces by using (3-aminopropyl)triethoxysilane (APTES). A replacement of conventionally casted membranes by the nanofibrous membranes increased the active surface area on the working electrode of a screen-printed three-electrode sensor by more than two times, consequently enhancing the fabricated aptasensor performance. The developed aptasensor demonstrated high sensitivity and specificity toward OTA in a range 0.002–2 ng mL
−1
, with a detection limit of 0.81 pg mL
−1
. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over long storage time.
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| subjects | Aminopropyltriethoxysilane Analytical Chemistry Cellulose Cellulose acetate Characterization and Evaluation of Materials Chemical Sciences Chemistry Chemistry and Materials Science Coffee Electric properties Electrodes Membranes Methylene blue Microengineering Nanochemistry Nanofibers Nanotechnology Original Paper Pretreatment Sensors Tethering |
| title | A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A |
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