Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer
Next‐generation sequencing (NGS) enables the diagnosis of large numbers of gene aberrations during one examination, and precision medicine has been developed for patients with advanced non–small cell lung cancer (NSCLC). However, peripheral lung lesions account for the majority of advanced lung canc...
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| Veröffentlicht in: | Cancer science 2021-01, Vol.112 (1), p.380-387 |
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| creator | Furuya, Naoki Matsumoto, Shingo Kakinuma, Kazutaka Morikawa, Kei Inoue, Takeo Saji, Hisashi Goto, Koichi Mineshita, Masamichi |
| description | Next‐generation sequencing (NGS) enables the diagnosis of large numbers of gene aberrations during one examination, and precision medicine has been developed for patients with advanced non–small cell lung cancer (NSCLC). However, peripheral lung lesions account for the majority of advanced lung cancers, especially lung adenocarcinoma. In these cases, it is difficult to obtain tissue samples which contain sufficient tumor cells by transbronchial biopsy (TBB) with forceps. Even when the target lesions are quite small, bronchial brushing can obtain enough tumor cells by endobronchial ultrasonography using guide sheath (EBUS‐GS). In this study, we investigate the suitability of bronchial brushing cytology specimens obtained by EBUS‐GS‐TBB to evaluate the correlation between the success rate of NGS and extracted DNA/RNA yields according to biopsy method. We prospectively collected 222 tumor samples obtained from patients with advanced lung cancer. All patients were enrolled in a prospective nationwide genomic screening project for lung cancer (LC‐SCRUM‐Japan/Asia). Genomic data were obtained from the clinico‐genomic database of LC‐SCRUM‐Japan/Asia. The extraction yields of DNA/RNA from samples obtained by EBUS‐GS‐TBB were relatively low compared with tissue samples. The success rate of DNA sequencing for EBUS‐GS‐TBB was 97.9%, with no significant differences between biopsy methods. The success rate of RNA sequencing for EBUS‐GS‐TBB was 80.4%, which was relatively low compared with surgical biopsy samples (P = 0.069). However, some rare oncogenic driver aberrations were detected from these specimens. This study demonstrated that cytology samples obtained by transbronchial brushing with EBUS‐GS‐TBB were suitable for NGS analysis.
This study demonstrated that cytology samples obtained by transbronchial brushing with endobronchial ultrasonography using guide sheath (EBUS‐GS) were suitable for next‐generation sequencing (NGS) analysis. We believe the new knowledge of this study can contribute to the best practice and further research for patients with advanced non–small cell lung cancer (NSCLC). |
| doi_str_mv | 10.1111/cas.14714 |
| format | Article |
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This study demonstrated that cytology samples obtained by transbronchial brushing with endobronchial ultrasonography using guide sheath (EBUS‐GS) were suitable for next‐generation sequencing (NGS) analysis. We believe the new knowledge of this study can contribute to the best practice and further research for patients with advanced non–small cell lung cancer (NSCLC).</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/cas.14714</identifier><identifier>PMID: 33124129</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Adenocarcinoma ; Adult ; Aged ; Aged, 80 and over ; Biopsy ; Biopsy, Needle - methods ; bronchial brushing ; Bronchoscopy ; Bronchoscopy - methods ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Cellular biology ; Clinical Research ; Cytology ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; Female ; High-Throughput Nucleotide Sequencing ; Histology ; Humans ; Image-Guided Biopsy - methods ; Laboratories ; Local anesthesia ; Lung cancer ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Lymphatic system ; Male ; Medicine ; Middle Aged ; Mutation ; Navigation systems ; next‐generation sequencing ; Non-small cell lung carcinoma ; nucleic acid yield ; Original ; Physiology ; Pleural effusion ; Precision medicine ; Ribonucleic acid ; RNA ; Small cell lung carcinoma ; Success ; success rate ; Tumor cells ; Ultrasonic imaging ; Ultrasonography, Interventional - methods</subject><ispartof>Cancer science, 2021-01, Vol.112 (1), p.380-387</ispartof><rights>2020 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5334-a80e94c82e388536aedb012dbb8d90ed6712776150e1ed14789ac2efb0c3e6f13</citedby><cites>FETCH-LOGICAL-c5334-a80e94c82e388536aedb012dbb8d90ed6712776150e1ed14789ac2efb0c3e6f13</cites><orcidid>0000-0002-3023-2510 ; 0000-0001-7162-5946</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780058/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780058/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33124129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furuya, Naoki</creatorcontrib><creatorcontrib>Matsumoto, Shingo</creatorcontrib><creatorcontrib>Kakinuma, Kazutaka</creatorcontrib><creatorcontrib>Morikawa, Kei</creatorcontrib><creatorcontrib>Inoue, Takeo</creatorcontrib><creatorcontrib>Saji, Hisashi</creatorcontrib><creatorcontrib>Goto, Koichi</creatorcontrib><creatorcontrib>Mineshita, Masamichi</creatorcontrib><title>Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>Next‐generation sequencing (NGS) enables the diagnosis of large numbers of gene aberrations during one examination, and precision medicine has been developed for patients with advanced non–small cell lung cancer (NSCLC). However, peripheral lung lesions account for the majority of advanced lung cancers, especially lung adenocarcinoma. In these cases, it is difficult to obtain tissue samples which contain sufficient tumor cells by transbronchial biopsy (TBB) with forceps. Even when the target lesions are quite small, bronchial brushing can obtain enough tumor cells by endobronchial ultrasonography using guide sheath (EBUS‐GS). In this study, we investigate the suitability of bronchial brushing cytology specimens obtained by EBUS‐GS‐TBB to evaluate the correlation between the success rate of NGS and extracted DNA/RNA yields according to biopsy method. We prospectively collected 222 tumor samples obtained from patients with advanced lung cancer. All patients were enrolled in a prospective nationwide genomic screening project for lung cancer (LC‐SCRUM‐Japan/Asia). Genomic data were obtained from the clinico‐genomic database of LC‐SCRUM‐Japan/Asia. The extraction yields of DNA/RNA from samples obtained by EBUS‐GS‐TBB were relatively low compared with tissue samples. The success rate of DNA sequencing for EBUS‐GS‐TBB was 97.9%, with no significant differences between biopsy methods. The success rate of RNA sequencing for EBUS‐GS‐TBB was 80.4%, which was relatively low compared with surgical biopsy samples (P = 0.069). However, some rare oncogenic driver aberrations were detected from these specimens. This study demonstrated that cytology samples obtained by transbronchial brushing with EBUS‐GS‐TBB were suitable for NGS analysis.
This study demonstrated that cytology samples obtained by transbronchial brushing with endobronchial ultrasonography using guide sheath (EBUS‐GS) were suitable for next‐generation sequencing (NGS) analysis. We believe the new knowledge of this study can contribute to the best practice and further research for patients with advanced non–small cell lung cancer (NSCLC).</description><subject>Adenocarcinoma</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biopsy</subject><subject>Biopsy, Needle - methods</subject><subject>bronchial brushing</subject><subject>Bronchoscopy</subject><subject>Bronchoscopy - methods</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Cellular biology</subject><subject>Clinical Research</subject><subject>Cytology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Female</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Histology</subject><subject>Humans</subject><subject>Image-Guided Biopsy - methods</subject><subject>Laboratories</subject><subject>Local anesthesia</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Lymphatic system</subject><subject>Male</subject><subject>Medicine</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Navigation systems</subject><subject>next‐generation sequencing</subject><subject>Non-small cell lung carcinoma</subject><subject>nucleic acid yield</subject><subject>Original</subject><subject>Physiology</subject><subject>Pleural effusion</subject><subject>Precision medicine</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Small cell lung carcinoma</subject><subject>Success</subject><subject>success rate</subject><subject>Tumor cells</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonography, Interventional - methods</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kcFuFSEUhomxsbW68AUMiRtdTAsDA8PGpLmp2qRJF9U1YZgz99JwYYQZdXZ9BJ_RJ5Hb2zZqIhtIzseXc86P0CtKTmg5p9bkE8ol5U_QEWVcVZIQ8fTuLStFWH2Inud8QwgTXPFn6JAxWnNaqyM0Xc9uMp3zblpwHPCUTMhdisFunPG4S3PeuLDGdpmij-sF5xGs20LIeIgJB_gx_br9uYYAyUwuBpzh6wzB7v64gEdIbtyUmsd-3mlMsJBeoIPB-Awv7-9j9OXD-efVp-ry6uPF6uyysg1jvDItAcVtWwNr24YJA31HaN13XdsrAr2QtJZS0IYAhb4soFXG1jB0xDIQA2XH6P3eO87dFnoLoYzn9Zjc1qRFR-P035XgNnodv2kpW0Katgje3gtSLGPlSW9dtuC9CRDnrGveCE6VFKSgb_5Bb-KcQhmvUJJL0SouCvVuT9kUc04wPDZDid5lqUuW-i7Lwr7-s_tH8iG8Apzuge_Ow_J_k16dXe-VvwF_xqz1</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Furuya, Naoki</creator><creator>Matsumoto, Shingo</creator><creator>Kakinuma, Kazutaka</creator><creator>Morikawa, Kei</creator><creator>Inoue, Takeo</creator><creator>Saji, Hisashi</creator><creator>Goto, Koichi</creator><creator>Mineshita, Masamichi</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3023-2510</orcidid><orcidid>https://orcid.org/0000-0001-7162-5946</orcidid></search><sort><creationdate>202101</creationdate><title>Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer</title><author>Furuya, Naoki ; Matsumoto, Shingo ; Kakinuma, Kazutaka ; Morikawa, Kei ; Inoue, Takeo ; Saji, Hisashi ; Goto, Koichi ; Mineshita, Masamichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5334-a80e94c82e388536aedb012dbb8d90ed6712776150e1ed14789ac2efb0c3e6f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adenocarcinoma</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biopsy</topic><topic>Biopsy, Needle - methods</topic><topic>bronchial brushing</topic><topic>Bronchoscopy</topic><topic>Bronchoscopy - methods</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Cellular biology</topic><topic>Clinical Research</topic><topic>Cytology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Female</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Histology</topic><topic>Humans</topic><topic>Image-Guided Biopsy - methods</topic><topic>Laboratories</topic><topic>Local anesthesia</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Lymphatic system</topic><topic>Male</topic><topic>Medicine</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Navigation systems</topic><topic>next‐generation sequencing</topic><topic>Non-small cell lung carcinoma</topic><topic>nucleic acid yield</topic><topic>Original</topic><topic>Physiology</topic><topic>Pleural effusion</topic><topic>Precision medicine</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Small cell lung carcinoma</topic><topic>Success</topic><topic>success rate</topic><topic>Tumor cells</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonography, Interventional - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furuya, Naoki</creatorcontrib><creatorcontrib>Matsumoto, Shingo</creatorcontrib><creatorcontrib>Kakinuma, Kazutaka</creatorcontrib><creatorcontrib>Morikawa, Kei</creatorcontrib><creatorcontrib>Inoue, Takeo</creatorcontrib><creatorcontrib>Saji, Hisashi</creatorcontrib><creatorcontrib>Goto, Koichi</creatorcontrib><creatorcontrib>Mineshita, Masamichi</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furuya, Naoki</au><au>Matsumoto, Shingo</au><au>Kakinuma, Kazutaka</au><au>Morikawa, Kei</au><au>Inoue, Takeo</au><au>Saji, Hisashi</au><au>Goto, Koichi</au><au>Mineshita, Masamichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2021-01</date><risdate>2021</risdate><volume>112</volume><issue>1</issue><spage>380</spage><epage>387</epage><pages>380-387</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>Next‐generation sequencing (NGS) enables the diagnosis of large numbers of gene aberrations during one examination, and precision medicine has been developed for patients with advanced non–small cell lung cancer (NSCLC). However, peripheral lung lesions account for the majority of advanced lung cancers, especially lung adenocarcinoma. In these cases, it is difficult to obtain tissue samples which contain sufficient tumor cells by transbronchial biopsy (TBB) with forceps. Even when the target lesions are quite small, bronchial brushing can obtain enough tumor cells by endobronchial ultrasonography using guide sheath (EBUS‐GS). In this study, we investigate the suitability of bronchial brushing cytology specimens obtained by EBUS‐GS‐TBB to evaluate the correlation between the success rate of NGS and extracted DNA/RNA yields according to biopsy method. We prospectively collected 222 tumor samples obtained from patients with advanced lung cancer. All patients were enrolled in a prospective nationwide genomic screening project for lung cancer (LC‐SCRUM‐Japan/Asia). Genomic data were obtained from the clinico‐genomic database of LC‐SCRUM‐Japan/Asia. The extraction yields of DNA/RNA from samples obtained by EBUS‐GS‐TBB were relatively low compared with tissue samples. The success rate of DNA sequencing for EBUS‐GS‐TBB was 97.9%, with no significant differences between biopsy methods. The success rate of RNA sequencing for EBUS‐GS‐TBB was 80.4%, which was relatively low compared with surgical biopsy samples (P = 0.069). However, some rare oncogenic driver aberrations were detected from these specimens. This study demonstrated that cytology samples obtained by transbronchial brushing with EBUS‐GS‐TBB were suitable for NGS analysis.
This study demonstrated that cytology samples obtained by transbronchial brushing with endobronchial ultrasonography using guide sheath (EBUS‐GS) were suitable for next‐generation sequencing (NGS) analysis. We believe the new knowledge of this study can contribute to the best practice and further research for patients with advanced non–small cell lung cancer (NSCLC).</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>33124129</pmid><doi>10.1111/cas.14714</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3023-2510</orcidid><orcidid>https://orcid.org/0000-0001-7162-5946</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma Adult Aged Aged, 80 and over Biopsy Biopsy, Needle - methods bronchial brushing Bronchoscopy Bronchoscopy - methods Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Cellular biology Clinical Research Cytology Deoxyribonucleic acid DNA DNA sequencing Female High-Throughput Nucleotide Sequencing Histology Humans Image-Guided Biopsy - methods Laboratories Local anesthesia Lung cancer Lung Neoplasms - genetics Lung Neoplasms - pathology Lymphatic system Male Medicine Middle Aged Mutation Navigation systems next‐generation sequencing Non-small cell lung carcinoma nucleic acid yield Original Physiology Pleural effusion Precision medicine Ribonucleic acid RNA Small cell lung carcinoma Success success rate Tumor cells Ultrasonic imaging Ultrasonography, Interventional - methods |
| title | Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer |
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