Expression of MiR-608 in Nonsmall Cell Lung Cancer and Molecular Mechanism of Apoptosis and Migration of A549 Cells
Objective. This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevan...
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| creator | Tian, Hui Lu, Ming Qi, Lei Si, Libo Gao, Cun Li, Shuhai Li, Lin Yue, Weiming Huang, Chu Cheng, Chuanle |
| description | Objective. This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results. MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion. The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment. |
| doi_str_mv | 10.1155/2020/8824519 |
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This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results. MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion. The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2020/8824519</identifier><identifier>PMID: 33426072</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>A549 Cells ; Analysis ; Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - genetics ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - metabolism ; Cell culture ; Cell migration ; Cell Movement - drug effects ; Cell Movement - genetics ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; Doxorubicin ; Doxorubicin - pharmacology ; Gastric cancer ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - genetics ; Genetic aspects ; Genetic polymorphisms ; Genotyping ; Health aspects ; Humans ; Identification and classification ; Lung cancer ; Lung cancer, Non-small cell ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Metastasis ; Methods ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Morbidity ; Non-small cell lung carcinoma ; Nucleotide sequencing ; Patients ; Physiological aspects ; Polymerase chain reaction ; Polymorphism ; Properties ; Proteins ; Ribonucleic acid ; RNA ; Surgery ; Tissues ; Tumors ; Western blotting ; Western immunoblotting</subject><ispartof>BioMed research international, 2020, Vol.2020 (2020), p.1-8</ispartof><rights>Copyright © 2020 Chu Huang et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Chu Huang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Chu Huang et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-3ee2448e96a432b58b188bcfe980d11622f5b49e312d9a5463c633f0289a9aba3</citedby><cites>FETCH-LOGICAL-c527t-3ee2448e96a432b58b188bcfe980d11622f5b49e312d9a5463c633f0289a9aba3</cites><orcidid>0000-0003-3564-1812 ; 0000-0003-4516-2313</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773458/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773458/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33426072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Xu, Zhenbo</contributor><contributor>Zhenbo Xu</contributor><creatorcontrib>Tian, Hui</creatorcontrib><creatorcontrib>Lu, Ming</creatorcontrib><creatorcontrib>Qi, Lei</creatorcontrib><creatorcontrib>Si, Libo</creatorcontrib><creatorcontrib>Gao, Cun</creatorcontrib><creatorcontrib>Li, Shuhai</creatorcontrib><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Yue, Weiming</creatorcontrib><creatorcontrib>Huang, Chu</creatorcontrib><creatorcontrib>Cheng, Chuanle</creatorcontrib><title>Expression of MiR-608 in Nonsmall Cell Lung Cancer and Molecular Mechanism of Apoptosis and Migration of A549 Cells</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Objective. This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results. MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion. The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment.</description><subject>A549 Cells</subject><subject>Analysis</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Cell culture</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Doxorubicin</subject><subject>Doxorubicin - pharmacology</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Genetic aspects</subject><subject>Genetic polymorphisms</subject><subject>Genotyping</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Lung cancer</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Metastasis</subject><subject>Methods</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Morbidity</subject><subject>Non-small cell lung carcinoma</subject><subject>Nucleotide sequencing</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Properties</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Tumors</subject><subject>Western blotting</subject><subject>Western immunoblotting</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkc1rFDEYhwdRbGl78ywBjzo23x8XYVlaFXYVRM8hk8nspswkYzJT2_--2e661YuYw5sX3icPb_hV1SsE3yPE2CWGGF5KiSlD6ll1igmiNUcUPT_2hJxUFznfwHIk4lDxl9UJIRRzKPBpla_uxuRy9jGA2IG1_1ZzKIEP4EsMeTB9D5aulNUcNmBpgnUJmNCCdeydnXuTwNrZrQk-D7v3izGOU8w-7yG_SWY6qBeMqkdXPq9edKbP7uJwn1U_rq--Lz_Vq68fPy8Xq9oyLKaaOIcplU5xQwlumGyQlI3tnJKwRYhj3LGGKkcQbpVhlBPLCekglsoo0xhyVn3Ye8e5GVxrXZiS6fWY_GDSvY7G678nwW_1Jt5qIQShTBbBm4MgxZ-zy5O-iXMKZWeNqaCSIwT5E7UxvdM-dLHI7OCz1QuuWDExwv5NScKogFQU6t2esinmnFx33BZBvUtc7xLXh8QL_vrPHx7h3_kW4O0e2PrQml_-P3WuMK4zTzQigitJHgAwlLkI</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Tian, Hui</creator><creator>Lu, Ming</creator><creator>Qi, Lei</creator><creator>Si, Libo</creator><creator>Gao, Cun</creator><creator>Li, Shuhai</creator><creator>Li, Lin</creator><creator>Yue, Weiming</creator><creator>Huang, Chu</creator><creator>Cheng, Chuanle</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3564-1812</orcidid><orcidid>https://orcid.org/0000-0003-4516-2313</orcidid></search><sort><creationdate>2020</creationdate><title>Expression of MiR-608 in Nonsmall Cell Lung Cancer and Molecular Mechanism of Apoptosis and Migration of A549 Cells</title><author>Tian, Hui ; Lu, Ming ; Qi, Lei ; Si, Libo ; Gao, Cun ; Li, Shuhai ; Li, Lin ; Yue, Weiming ; Huang, Chu ; Cheng, Chuanle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-3ee2448e96a432b58b188bcfe980d11622f5b49e312d9a5463c633f0289a9aba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>A549 Cells</topic><topic>Analysis</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Cell culture</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Doxorubicin</topic><topic>Doxorubicin - pharmacology</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Genetic aspects</topic><topic>Genetic polymorphisms</topic><topic>Genotyping</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Lung cancer</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Metastasis</topic><topic>Methods</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Morbidity</topic><topic>Non-small cell lung carcinoma</topic><topic>Nucleotide sequencing</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Properties</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Surgery</topic><topic>Tissues</topic><topic>Tumors</topic><topic>Western blotting</topic><topic>Western immunoblotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Hui</creatorcontrib><creatorcontrib>Lu, Ming</creatorcontrib><creatorcontrib>Qi, Lei</creatorcontrib><creatorcontrib>Si, Libo</creatorcontrib><creatorcontrib>Gao, Cun</creatorcontrib><creatorcontrib>Li, Shuhai</creatorcontrib><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Yue, Weiming</creatorcontrib><creatorcontrib>Huang, Chu</creatorcontrib><creatorcontrib>Cheng, Chuanle</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results. MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion. The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33426072</pmid><doi>10.1155/2020/8824519</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3564-1812</orcidid><orcidid>https://orcid.org/0000-0003-4516-2313</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | A549 Cells Analysis Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Apoptosis - genetics Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - metabolism Cell culture Cell migration Cell Movement - drug effects Cell Movement - genetics Deoxyribonucleic acid DNA DNA sequencing Doxorubicin Doxorubicin - pharmacology Gastric cancer Gene expression Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - genetics Genetic aspects Genetic polymorphisms Genotyping Health aspects Humans Identification and classification Lung cancer Lung cancer, Non-small cell Lung Neoplasms - genetics Lung Neoplasms - metabolism Metastasis Methods MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Morbidity Non-small cell lung carcinoma Nucleotide sequencing Patients Physiological aspects Polymerase chain reaction Polymorphism Properties Proteins Ribonucleic acid RNA Surgery Tissues Tumors Western blotting Western immunoblotting |
| title | Expression of MiR-608 in Nonsmall Cell Lung Cancer and Molecular Mechanism of Apoptosis and Migration of A549 Cells |
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