Antimicrobial properties of diethylamine NONOate, a nitric oxide donor, against Escherichia coli: a pilot study
The emergence of SARS-CoV-2, the causative agent of COVID-19, highlights the increasing need for new and effective antiviral and antimicrobial agents. The FDA has recently banned several active ingredients used in hand sanitizers, including triclosan and benzethonium chloride. Nitric oxide (NO) is i...
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| Veröffentlicht in: | Journal of antibiotics 2021-04, Vol.74 (4), p.260-265 |
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| creator | Sysel, Annette M. Dunphy, Michael J. Bauer, Joseph A. |
| description | The emergence of SARS-CoV-2, the causative agent of COVID-19, highlights the increasing need for new and effective antiviral and antimicrobial agents. The FDA has recently banned several active ingredients used in hand sanitizers, including triclosan and benzethonium chloride. Nitric oxide (NO) is involved in the innate immune response and is a major component of macrophage-mediated attack on foreign viruses and bacteria. The specific aim of this study was to assess the antibacterial effects of 2-(
N
,
N
-diethylamino)-diazenolate-2-oxide (DEA-NONOate) against
Escherichia coli
(
E. coli
). A bacterial growth assay was compared to an adenosine triphosphate (ATP) activity assay at various time points to assess effects of DEA-NONOate on
E. coli
growth. A UV/Vis spectrophotometer was used to determine concentration of
E. coli
by measuring optical density (OD) at 630 nm. A luminescent assay was used to measure ATP activity correlating to viable cells. DEA-NONOate at a concentration of 65 mM was able to inhibit the growth of
E. coli
with the same efficacy as 1 μg ml
−1
concentration of ciprofloxacin. Both the OD and ATP assays demonstrated a 99.9% reduction in
E. coli
. Both a 1 μg ml
−1
concentration of ciprofloxacin and a 65 mM concentration of DEA-NONOate achieved 99.9% inhibition of
E. coli
, verified using both optical density measurement of bacterial cultures in 96 well plates and a luminescent ATP activity assay. The bactericidal effects of DEA-NONOate against
E. coli
is proof-of-concept to pursue evaluation of nitric oxide-based formulations as antimicrobial and antiviral agents as hand sanitizers. |
| doi_str_mv | 10.1038/s41429-020-00397-9 |
| format | Article |
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N
,
N
-diethylamino)-diazenolate-2-oxide (DEA-NONOate) against
Escherichia coli
(
E. coli
). A bacterial growth assay was compared to an adenosine triphosphate (ATP) activity assay at various time points to assess effects of DEA-NONOate on
E. coli
growth. A UV/Vis spectrophotometer was used to determine concentration of
E. coli
by measuring optical density (OD) at 630 nm. A luminescent assay was used to measure ATP activity correlating to viable cells. DEA-NONOate at a concentration of 65 mM was able to inhibit the growth of
E. coli
with the same efficacy as 1 μg ml
−1
concentration of ciprofloxacin. Both the OD and ATP assays demonstrated a 99.9% reduction in
E. coli
. Both a 1 μg ml
−1
concentration of ciprofloxacin and a 65 mM concentration of DEA-NONOate achieved 99.9% inhibition of
E. coli
, verified using both optical density measurement of bacterial cultures in 96 well plates and a luminescent ATP activity assay. The bactericidal effects of DEA-NONOate against
E. coli
is proof-of-concept to pursue evaluation of nitric oxide-based formulations as antimicrobial and antiviral agents as hand sanitizers.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/s41429-020-00397-9</identifier><identifier>PMID: 33361779</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/106 ; 14/5 ; 631/326/2522 ; 692/420/254 ; Adenosine triphosphate ; Adenosine Triphosphate - metabolism ; Anti-Bacterial Agents - pharmacology ; Antimicrobial agents ; Antiviral agents ; ATP ; Bacteriology ; Biomedical and Life Sciences ; Bioorganic Chemistry ; Ciprofloxacin - pharmacology ; COVID-19 ; Density measurement ; E coli ; Escherichia coli - drug effects ; Hand Sanitizers - chemistry ; Humans ; Hydrazines - pharmacology ; Immune response ; Life Sciences ; Luminescent Measurements ; Medicinal Chemistry ; Microbiology ; Nitric oxide ; Organic Chemistry ; Pilot Projects ; Sanitizers ; Severe acute respiratory syndrome coronavirus 2 ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of antibiotics, 2021-04, Vol.74 (4), p.260-265</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2020</rights><rights>The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-36ffeddc3279415207d764dea83c6606a967a9931a8ec824e21a18513bf57bbc3</citedby><cites>FETCH-LOGICAL-c498t-36ffeddc3279415207d764dea83c6606a967a9931a8ec824e21a18513bf57bbc3</cites><orcidid>0000-0003-4972-6008</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33361779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sysel, Annette M.</creatorcontrib><creatorcontrib>Dunphy, Michael J.</creatorcontrib><creatorcontrib>Bauer, Joseph A.</creatorcontrib><title>Antimicrobial properties of diethylamine NONOate, a nitric oxide donor, against Escherichia coli: a pilot study</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>The emergence of SARS-CoV-2, the causative agent of COVID-19, highlights the increasing need for new and effective antiviral and antimicrobial agents. The FDA has recently banned several active ingredients used in hand sanitizers, including triclosan and benzethonium chloride. Nitric oxide (NO) is involved in the innate immune response and is a major component of macrophage-mediated attack on foreign viruses and bacteria. The specific aim of this study was to assess the antibacterial effects of 2-(
N
,
N
-diethylamino)-diazenolate-2-oxide (DEA-NONOate) against
Escherichia coli
(
E. coli
). A bacterial growth assay was compared to an adenosine triphosphate (ATP) activity assay at various time points to assess effects of DEA-NONOate on
E. coli
growth. A UV/Vis spectrophotometer was used to determine concentration of
E. coli
by measuring optical density (OD) at 630 nm. A luminescent assay was used to measure ATP activity correlating to viable cells. DEA-NONOate at a concentration of 65 mM was able to inhibit the growth of
E. coli
with the same efficacy as 1 μg ml
−1
concentration of ciprofloxacin. Both the OD and ATP assays demonstrated a 99.9% reduction in
E. coli
. Both a 1 μg ml
−1
concentration of ciprofloxacin and a 65 mM concentration of DEA-NONOate achieved 99.9% inhibition of
E. coli
, verified using both optical density measurement of bacterial cultures in 96 well plates and a luminescent ATP activity assay. The bactericidal effects of DEA-NONOate against
E. coli
is proof-of-concept to pursue evaluation of nitric oxide-based formulations as antimicrobial and antiviral agents as hand sanitizers.</description><subject>13/1</subject><subject>13/106</subject><subject>14/5</subject><subject>631/326/2522</subject><subject>692/420/254</subject><subject>Adenosine triphosphate</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Antiviral agents</subject><subject>ATP</subject><subject>Bacteriology</subject><subject>Biomedical and Life Sciences</subject><subject>Bioorganic Chemistry</subject><subject>Ciprofloxacin - pharmacology</subject><subject>COVID-19</subject><subject>Density measurement</subject><subject>E coli</subject><subject>Escherichia coli - drug effects</subject><subject>Hand Sanitizers - chemistry</subject><subject>Humans</subject><subject>Hydrazines - pharmacology</subject><subject>Immune response</subject><subject>Life Sciences</subject><subject>Luminescent Measurements</subject><subject>Medicinal Chemistry</subject><subject>Microbiology</subject><subject>Nitric oxide</subject><subject>Organic Chemistry</subject><subject>Pilot Projects</subject><subject>Sanitizers</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtv1DAUhS0EotOBP8ACWWLDogE_Ej9YVKqq8pCqzgbWluPczLhK7MF2EPPvcZlSHgtWlny-e3yuD0IvKHlDCVdvc0tbphvCSEMI17LRj9CKKkUb2gr9GK0IYbRRipETdJrzbYUkl-opOuGcCyqlXqF4EYqfvUux93bC-xT3kIqHjOOIBw9ld5js7APgm83NxhY4wxYHX5J3OH73A-Ahhpjq7db6kAu-ym4HVd15i12c_LvK7_0UC85lGQ7P0JPRThme359r9OX91efLj8315sOny4vrxrValYaLcYRhcJxJ3dKOETlI0Q5gFXdCEGG1kFZrTq0Cp1gLjFqqOsr7sZN97_ganR9990s_w-AglGQns09-tulgovXmbyX4ndnGb0ZKIQVX1eD1vUGKXxfIxcw-O5gmGyAu2bBWcn6XoKvoq3_Q27ikUNczrCOC0q6rydaIHan61zknGB_CUGLu-jTHPk3t0_zs0-g69PLPNR5GfhVYAX4EcpXCFtLvt_9j-wNOBayh</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Sysel, Annette M.</creator><creator>Dunphy, Michael J.</creator><creator>Bauer, Joseph A.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4972-6008</orcidid></search><sort><creationdate>20210401</creationdate><title>Antimicrobial properties of diethylamine NONOate, a nitric oxide donor, against Escherichia coli: a pilot study</title><author>Sysel, Annette M. ; 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The FDA has recently banned several active ingredients used in hand sanitizers, including triclosan and benzethonium chloride. Nitric oxide (NO) is involved in the innate immune response and is a major component of macrophage-mediated attack on foreign viruses and bacteria. The specific aim of this study was to assess the antibacterial effects of 2-(
N
,
N
-diethylamino)-diazenolate-2-oxide (DEA-NONOate) against
Escherichia coli
(
E. coli
). A bacterial growth assay was compared to an adenosine triphosphate (ATP) activity assay at various time points to assess effects of DEA-NONOate on
E. coli
growth. A UV/Vis spectrophotometer was used to determine concentration of
E. coli
by measuring optical density (OD) at 630 nm. A luminescent assay was used to measure ATP activity correlating to viable cells. DEA-NONOate at a concentration of 65 mM was able to inhibit the growth of
E. coli
with the same efficacy as 1 μg ml
−1
concentration of ciprofloxacin. Both the OD and ATP assays demonstrated a 99.9% reduction in
E. coli
. Both a 1 μg ml
−1
concentration of ciprofloxacin and a 65 mM concentration of DEA-NONOate achieved 99.9% inhibition of
E. coli
, verified using both optical density measurement of bacterial cultures in 96 well plates and a luminescent ATP activity assay. The bactericidal effects of DEA-NONOate against
E. coli
is proof-of-concept to pursue evaluation of nitric oxide-based formulations as antimicrobial and antiviral agents as hand sanitizers.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33361779</pmid><doi>10.1038/s41429-020-00397-9</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-4972-6008</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of antibiotics, 2021-04, Vol.74 (4), p.260-265 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 13/1 13/106 14/5 631/326/2522 692/420/254 Adenosine triphosphate Adenosine Triphosphate - metabolism Anti-Bacterial Agents - pharmacology Antimicrobial agents Antiviral agents ATP Bacteriology Biomedical and Life Sciences Bioorganic Chemistry Ciprofloxacin - pharmacology COVID-19 Density measurement E coli Escherichia coli - drug effects Hand Sanitizers - chemistry Humans Hydrazines - pharmacology Immune response Life Sciences Luminescent Measurements Medicinal Chemistry Microbiology Nitric oxide Organic Chemistry Pilot Projects Sanitizers Severe acute respiratory syndrome coronavirus 2 Spectrophotometry, Ultraviolet |
| title | Antimicrobial properties of diethylamine NONOate, a nitric oxide donor, against Escherichia coli: a pilot study |
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