Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection
Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant (r- ) expressing a novel...
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| Veröffentlicht in: | Viruses 2020-11, Vol.12 (12), p.1350 |
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| creator | Wang, Zhihao Mi, Jielan Wang, Yulong Wang, Tingting Qi, Xiaole Li, Kai Pan, Qing Gao, Yulong Gao, Li Liu, Changjun Zhang, Yanping Wang, Xiaomei Cui, Hongyu |
| description | Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant
(r-
) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-
successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-
-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-
-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain. |
| doi_str_mv | 10.3390/v12121350 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7760868</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2466036981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-c5af1fcabf11fbdb61c8b4d905072fb90b254b4ce7b64f843019b26544b43d503</originalsourceid><addsrcrecordid>eNpdks1u1DAUhSMEoj-w4AWQJTbtYqgd_yTeIJVpgUqjUgGdbWQ7161LYgc7GQFPx6PhaNpRQV7Yuv587vHVKYpXBL-lVOKTDSnzohw_KfaJlHLBJOFPH533ioOU7jAWQuLqebFHacl5xcr94s8XMKHXzis_opUyYzDBmCmh859DhJScv0EKXYYNdGitopuxYNGFt2BGFzL4fopJdejMJVAJ0NrFXFxflegqhhGcR0vlM99OBtC1dz8mQF8HMM46gy5hGqPq3O-5zakfnQ6tg4TmV7fOfAefkPLtLLVxLaBl6IcORthqzwb8i-KZVV2Cl_f7YXH94fzb8tNi9fnjxfJ0tTAM03FhuLLEGqUtIVa3WhBTa9ZKzHFVWi2xLjnTzEClBbM1o5hIXQrOcpG2HNPD4t1Wd5h0D60BPztvhuh6FX81Qbnm3xvvbpubsGmqSuBa1Fng6F4ghjyENDa9Swa6TnnIc2xKJgSmQtYko2_-Q-_CFH3-3kxxSmrCWKaOt5SJIaUIdmeG4GbORbPLRWZfP3a_Ix-CQP8CIK22-w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2465318144</pqid></control><display><type>article</type><title>Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wang, Zhihao ; Mi, Jielan ; Wang, Yulong ; Wang, Tingting ; Qi, Xiaole ; Li, Kai ; Pan, Qing ; Gao, Yulong ; Gao, Li ; Liu, Changjun ; Zhang, Yanping ; Wang, Xiaomei ; Cui, Hongyu</creator><creatorcontrib>Wang, Zhihao ; Mi, Jielan ; Wang, Yulong ; Wang, Tingting ; Qi, Xiaole ; Li, Kai ; Pan, Qing ; Gao, Yulong ; Gao, Li ; Liu, Changjun ; Zhang, Yanping ; Wang, Xiaomei ; Cui, Hongyu</creatorcontrib><description>Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant
(r-
) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-
successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-
-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-
-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain.</description><identifier>ISSN: 1999-4915</identifier><identifier>EISSN: 1999-4915</identifier><identifier>DOI: 10.3390/v12121350</identifier><identifier>PMID: 33255742</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Antibodies ; Antibodies, Neutralizing - immunology ; Antibodies, Viral - immunology ; Antigens ; Bacteria ; Biomarkers ; Biotechnology industry ; Birnaviridae Infections - veterinary ; Chickens - immunology ; Cytokines - blood ; Cytokines - metabolism ; Enzyme-Linked Immunosorbent Assay ; Fusion protein ; Gene Expression ; Gram-positive bacteria ; Immune system ; Immunization ; Infections ; Infectious bursal disease virus - immunology ; Laboratories ; Lactococcus - genetics ; Neutralization Tests ; Plasmids - genetics ; Poultry ; Poultry Diseases - immunology ; Poultry Diseases - prevention & control ; Poultry Diseases - virology ; Proteins ; Recombinant Proteins ; Salmonella ; Vaccines ; Viral Structural Proteins - genetics ; Viral Structural Proteins - immunology ; Viral Vaccines - immunology ; Virulence ; VP2 protein ; Western blotting</subject><ispartof>Viruses, 2020-11, Vol.12 (12), p.1350</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-c5af1fcabf11fbdb61c8b4d905072fb90b254b4ce7b64f843019b26544b43d503</citedby><cites>FETCH-LOGICAL-c403t-c5af1fcabf11fbdb61c8b4d905072fb90b254b4ce7b64f843019b26544b43d503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760868/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760868/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33255742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Zhihao</creatorcontrib><creatorcontrib>Mi, Jielan</creatorcontrib><creatorcontrib>Wang, Yulong</creatorcontrib><creatorcontrib>Wang, Tingting</creatorcontrib><creatorcontrib>Qi, Xiaole</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Pan, Qing</creatorcontrib><creatorcontrib>Gao, Yulong</creatorcontrib><creatorcontrib>Gao, Li</creatorcontrib><creatorcontrib>Liu, Changjun</creatorcontrib><creatorcontrib>Zhang, Yanping</creatorcontrib><creatorcontrib>Wang, Xiaomei</creatorcontrib><creatorcontrib>Cui, Hongyu</creatorcontrib><title>Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection</title><title>Viruses</title><addtitle>Viruses</addtitle><description>Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant
(r-
) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-
successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-
-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-
-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Neutralizing - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens</subject><subject>Bacteria</subject><subject>Biomarkers</subject><subject>Biotechnology industry</subject><subject>Birnaviridae Infections - veterinary</subject><subject>Chickens - immunology</subject><subject>Cytokines - blood</subject><subject>Cytokines - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fusion protein</subject><subject>Gene Expression</subject><subject>Gram-positive bacteria</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Infections</subject><subject>Infectious bursal disease virus - immunology</subject><subject>Laboratories</subject><subject>Lactococcus - genetics</subject><subject>Neutralization Tests</subject><subject>Plasmids - genetics</subject><subject>Poultry</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Poultry Diseases - virology</subject><subject>Proteins</subject><subject>Recombinant Proteins</subject><subject>Salmonella</subject><subject>Vaccines</subject><subject>Viral Structural Proteins - genetics</subject><subject>Viral Structural Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virulence</subject><subject>VP2 protein</subject><subject>Western blotting</subject><issn>1999-4915</issn><issn>1999-4915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdks1u1DAUhSMEoj-w4AWQJTbtYqgd_yTeIJVpgUqjUgGdbWQ7161LYgc7GQFPx6PhaNpRQV7Yuv587vHVKYpXBL-lVOKTDSnzohw_KfaJlHLBJOFPH533ioOU7jAWQuLqebFHacl5xcr94s8XMKHXzis_opUyYzDBmCmh859DhJScv0EKXYYNdGitopuxYNGFt2BGFzL4fopJdejMJVAJ0NrFXFxflegqhhGcR0vlM99OBtC1dz8mQF8HMM46gy5hGqPq3O-5zakfnQ6tg4TmV7fOfAefkPLtLLVxLaBl6IcORthqzwb8i-KZVV2Cl_f7YXH94fzb8tNi9fnjxfJ0tTAM03FhuLLEGqUtIVa3WhBTa9ZKzHFVWi2xLjnTzEClBbM1o5hIXQrOcpG2HNPD4t1Wd5h0D60BPztvhuh6FX81Qbnm3xvvbpubsGmqSuBa1Fng6F4ghjyENDa9Swa6TnnIc2xKJgSmQtYko2_-Q-_CFH3-3kxxSmrCWKaOt5SJIaUIdmeG4GbORbPLRWZfP3a_Ix-CQP8CIK22-w</recordid><startdate>20201125</startdate><enddate>20201125</enddate><creator>Wang, Zhihao</creator><creator>Mi, Jielan</creator><creator>Wang, Yulong</creator><creator>Wang, Tingting</creator><creator>Qi, Xiaole</creator><creator>Li, Kai</creator><creator>Pan, Qing</creator><creator>Gao, Yulong</creator><creator>Gao, Li</creator><creator>Liu, Changjun</creator><creator>Zhang, Yanping</creator><creator>Wang, Xiaomei</creator><creator>Cui, Hongyu</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201125</creationdate><title>Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection</title><author>Wang, Zhihao ; Mi, Jielan ; Wang, Yulong ; Wang, Tingting ; Qi, Xiaole ; Li, Kai ; Pan, Qing ; Gao, Yulong ; Gao, Li ; Liu, Changjun ; Zhang, Yanping ; Wang, Xiaomei ; Cui, Hongyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-c5af1fcabf11fbdb61c8b4d905072fb90b254b4ce7b64f843019b26544b43d503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Neutralizing - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens</topic><topic>Bacteria</topic><topic>Biomarkers</topic><topic>Biotechnology industry</topic><topic>Birnaviridae Infections - veterinary</topic><topic>Chickens - immunology</topic><topic>Cytokines - blood</topic><topic>Cytokines - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fusion protein</topic><topic>Gene Expression</topic><topic>Gram-positive bacteria</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Infections</topic><topic>Infectious bursal disease virus - immunology</topic><topic>Laboratories</topic><topic>Lactococcus - genetics</topic><topic>Neutralization Tests</topic><topic>Plasmids - genetics</topic><topic>Poultry</topic><topic>Poultry Diseases - immunology</topic><topic>Poultry Diseases - prevention & control</topic><topic>Poultry Diseases - virology</topic><topic>Proteins</topic><topic>Recombinant Proteins</topic><topic>Salmonella</topic><topic>Vaccines</topic><topic>Viral Structural Proteins - genetics</topic><topic>Viral Structural Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virulence</topic><topic>VP2 protein</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhihao</creatorcontrib><creatorcontrib>Mi, Jielan</creatorcontrib><creatorcontrib>Wang, Yulong</creatorcontrib><creatorcontrib>Wang, Tingting</creatorcontrib><creatorcontrib>Qi, Xiaole</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Pan, Qing</creatorcontrib><creatorcontrib>Gao, Yulong</creatorcontrib><creatorcontrib>Gao, Li</creatorcontrib><creatorcontrib>Liu, Changjun</creatorcontrib><creatorcontrib>Zhang, Yanping</creatorcontrib><creatorcontrib>Wang, Xiaomei</creatorcontrib><creatorcontrib>Cui, Hongyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Viruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhihao</au><au>Mi, Jielan</au><au>Wang, Yulong</au><au>Wang, Tingting</au><au>Qi, Xiaole</au><au>Li, Kai</au><au>Pan, Qing</au><au>Gao, Yulong</au><au>Gao, Li</au><au>Liu, Changjun</au><au>Zhang, Yanping</au><au>Wang, Xiaomei</au><au>Cui, Hongyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection</atitle><jtitle>Viruses</jtitle><addtitle>Viruses</addtitle><date>2020-11-25</date><risdate>2020</risdate><volume>12</volume><issue>12</issue><spage>1350</spage><pages>1350-</pages><issn>1999-4915</issn><eissn>1999-4915</eissn><abstract>Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant
(r-
) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-
successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-
-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-
-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33255742</pmid><doi>10.3390/v12121350</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Antibodies Antibodies, Neutralizing - immunology Antibodies, Viral - immunology Antigens Bacteria Biomarkers Biotechnology industry Birnaviridae Infections - veterinary Chickens - immunology Cytokines - blood Cytokines - metabolism Enzyme-Linked Immunosorbent Assay Fusion protein Gene Expression Gram-positive bacteria Immune system Immunization Infections Infectious bursal disease virus - immunology Laboratories Lactococcus - genetics Neutralization Tests Plasmids - genetics Poultry Poultry Diseases - immunology Poultry Diseases - prevention & control Poultry Diseases - virology Proteins Recombinant Proteins Salmonella Vaccines Viral Structural Proteins - genetics Viral Structural Proteins - immunology Viral Vaccines - immunology Virulence VP2 protein Western blotting |
| title | Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection |
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