Quantitative evaluation of human sperm viability using MTT assay: A laboratory study
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability. This study was performed to assess human sperm viability using MTT assay. In this laboratory study, human...
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| Veröffentlicht in: | International Journal of Reproductive BioMedicine 2020-11, Vol.18 (11), p.983-988 |
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| container_title | International Journal of Reproductive BioMedicine |
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| creator | Momeni, Hamid Reza Abnosi, Mohammad Hussein Eskandari, Najmeh |
| description | 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
This study was performed to assess human sperm viability using MTT assay.
In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings. |
| doi_str_mv | 10.18502/ijrm.v13i11.7966 |
| format | Article |
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This study was performed to assess human sperm viability using MTT assay.
In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.</description><identifier>ISSN: 2476-4108</identifier><identifier>EISSN: 2476-3772</identifier><identifier>DOI: 10.18502/ijrm.v13i11.7966</identifier><identifier>PMID: 33349807</identifier><language>eng</language><publisher>Iran: Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</publisher><subject>human sperm, viability, mtt assay ; Laboratories ; Motility ; Short Communication ; Sperm</subject><ispartof>International Journal of Reproductive BioMedicine, 2020-11, Vol.18 (11), p.983-988</ispartof><rights>Copyright © 2020 Momeni et al.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020 Momeni et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-a228c92cd0b2af9809043bde09d3abe6f120884d5590981659ddffc2163876033</citedby><cites>FETCH-LOGICAL-c493t-a228c92cd0b2af9809043bde09d3abe6f120884d5590981659ddffc2163876033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749972/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749972/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33349807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Momeni, Hamid Reza</creatorcontrib><creatorcontrib>Abnosi, Mohammad Hussein</creatorcontrib><creatorcontrib>Eskandari, Najmeh</creatorcontrib><title>Quantitative evaluation of human sperm viability using MTT assay: A laboratory study</title><title>International Journal of Reproductive BioMedicine</title><addtitle>Int J Reprod Biomed</addtitle><description>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
This study was performed to assess human sperm viability using MTT assay.
In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.</description><subject>human sperm, viability, mtt assay</subject><subject>Laboratories</subject><subject>Motility</subject><subject>Short Communication</subject><subject>Sperm</subject><issn>2476-4108</issn><issn>2476-3772</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNpdkktr3DAUhU1paUKaH9BNEXTTzUz0sPXoohBCH4GUUpiuxbUkTzTY1lSSDf730WSmIelKF-ncT0e6p6reE7wmssH0yu_isJ4J84SsheL8VXVOa8FXTAj6-lTXBMuz6jKlHcaY8IYQyt5WZ4yxWkkszqvN7wnG7DNkPzvkZuinUoYRhQ7dTwOMKO1dHNDsofW9zwuakh-36OdmgyAlWD6ja9RDGyLkEBeU8mSXd9WbDvrkLk_rRfXn29fNzY_V3a_vtzfXdytTK5ZXQKk0ihqLWwpd8aNwzVrrsLIMWsc7QrGUtW0ahZUs7pW1XWco4UwKjhm7qG6PXBtgp_fRDxAXHcDrx40Qtxpi9qZ3WgjScku5sJLW2DgoeIHLNdQ2prOisL4cWfupHZw1bswR-hfQlyejv9fbMBdyrZSgBfDpBIjh7-RS1oNPxvU9jC5MSZd5UIKbmvMi_fifdBemOJav0pRLKnEZzsEROapMDClF1z2ZIVg_RkAfIqCPEdCHCJSeD89f8dTxb-DsAbCsrmI</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Momeni, Hamid Reza</creator><creator>Abnosi, Mohammad Hussein</creator><creator>Eskandari, Najmeh</creator><general>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</general><general>Knowledge E</general><general>Shahid Sadoughi University of Medical Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20201101</creationdate><title>Quantitative evaluation of human sperm viability using MTT assay: A laboratory study</title><author>Momeni, Hamid Reza ; Abnosi, Mohammad Hussein ; Eskandari, Najmeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-a228c92cd0b2af9809043bde09d3abe6f120884d5590981659ddffc2163876033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>human sperm, viability, mtt assay</topic><topic>Laboratories</topic><topic>Motility</topic><topic>Short Communication</topic><topic>Sperm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Momeni, Hamid Reza</creatorcontrib><creatorcontrib>Abnosi, Mohammad Hussein</creatorcontrib><creatorcontrib>Eskandari, Najmeh</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International Journal of Reproductive BioMedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Momeni, Hamid Reza</au><au>Abnosi, Mohammad Hussein</au><au>Eskandari, Najmeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative evaluation of human sperm viability using MTT assay: A laboratory study</atitle><jtitle>International Journal of Reproductive BioMedicine</jtitle><addtitle>Int J Reprod Biomed</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>18</volume><issue>11</issue><spage>983</spage><epage>988</epage><pages>983-988</pages><issn>2476-4108</issn><eissn>2476-3772</eissn><abstract>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
This study was performed to assess human sperm viability using MTT assay.
In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.</abstract><cop>Iran</cop><pub>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</pub><pmid>33349807</pmid><doi>10.18502/ijrm.v13i11.7966</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | human sperm, viability, mtt assay Laboratories Motility Short Communication Sperm |
| title | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
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