Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study
The role of and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of...
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| Veröffentlicht in: | International Journal of Reproductive BioMedicine 2020-11, Vol.18 (11), p.961-968 |
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| container_title | International Journal of Reproductive BioMedicine |
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| creator | Norioun, Hamid Motovali-Bashi, Majid Javadirad, Seyed Morteza |
| description | The role of
and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of
is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.
The aim of this study was to evaluate the expression level of
in azoospermic men to reveal its possible association with infertility.
This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and
was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR) method was used and statistical analysis was performed to compare the expression level of
in both OA and NOA individuals.
In silico analysis suggested
, with its potential binding ability to target
transcripts. The expression analysis of candidate
indicated its significant overexpression in NOA men.
The
was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly
and indirectly
and
. Therefore, spermatogenesis could be misled and male infertility could be developed. |
| doi_str_mv | 10.18502/ijrm.v13i11.7963 |
| format | Article |
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and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of
is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.
The aim of this study was to evaluate the expression level of
in azoospermic men to reveal its possible association with infertility.
This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and
was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR) method was used and statistical analysis was performed to compare the expression level of
in both OA and NOA individuals.
In silico analysis suggested
, with its potential binding ability to target
transcripts. The expression analysis of candidate
indicated its significant overexpression in NOA men.
The
was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly
and indirectly
and
. Therefore, spermatogenesis could be misled and male infertility could be developed.</description><identifier>ISSN: 2476-4108</identifier><identifier>EISSN: 2476-3772</identifier><identifier>DOI: 10.18502/ijrm.v13i11.7963</identifier><identifier>PMID: 33349804</identifier><language>eng</language><publisher>Iran: Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</publisher><subject>hsa-mir-27a-3p, male infertility, kdm3a ; Infertility ; Male reproductive system ; Mens health ; Spermatogenesis</subject><ispartof>International Journal of Reproductive BioMedicine, 2020-11, Vol.18 (11), p.961-968</ispartof><rights>Copyright © 2020 Norioun et al.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020 Norioun et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-c67368170d342e9442aa895c1bd49934ec72cdbd0dcdcb559a29649c3e44825c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749971/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749971/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33349804$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Norioun, Hamid</creatorcontrib><creatorcontrib>Motovali-Bashi, Majid</creatorcontrib><creatorcontrib>Javadirad, Seyed Morteza</creatorcontrib><title>Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study</title><title>International Journal of Reproductive BioMedicine</title><addtitle>Int J Reprod Biomed</addtitle><description>The role of
and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of
is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.
The aim of this study was to evaluate the expression level of
in azoospermic men to reveal its possible association with infertility.
This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and
was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR) method was used and statistical analysis was performed to compare the expression level of
in both OA and NOA individuals.
In silico analysis suggested
, with its potential binding ability to target
transcripts. The expression analysis of candidate
indicated its significant overexpression in NOA men.
The
was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly
and indirectly
and
. Therefore, spermatogenesis could be misled and male infertility could be developed.</description><subject>hsa-mir-27a-3p, male infertility, kdm3a</subject><subject>Infertility</subject><subject>Male reproductive system</subject><subject>Mens health</subject><subject>Spermatogenesis</subject><issn>2476-4108</issn><issn>2476-3772</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNpdkk9v1DAQxSMEolXpB-CCInHhksX_EtsckKoKaKVKSAguXKzJeLb1KokXO1konx63u1SUky3Pm59m_F5VveRsxU3LxNuwSeNqx2XgfKVtJ59Ux0LprpFai6eHu-LMHFWnOW8YY7xrORfyeXUkpVTWMHVcfb_I0IzhSyM0NHJbxx0l-rVNlHOIUx2meqSp_hnmm3qKU-zznBacw45q-B1j3lIaA7yrz2qETA3GaU5xqPO8-NsX1bM1DJlOD-dJ9e3jh6_nF83V50-X52dXDSor5wY7LTvDNfNSCbJKCQBjW-S9V9ZKRagF-t4zjx77trUgbKcsSlLKiBblSXW55_oIG7dNYYR06yIEd_8Q07WDNAccyGneghRMIaJQwCzYNTPAvFmbXnTECuv9nrVd-pE8UtkHhkfQx5Up3LjruHNal2E1L4A3B0CKPxbKsxtDRhoGmCgu2RVXRPFEcF2kr_-TbuKSpvJVTnRGFHuMlUXF9ypMMedE64dhOHP3QXB3QXD7ILi7IJSeV_9u8dDx13b5B0xtsAY</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Norioun, Hamid</creator><creator>Motovali-Bashi, Majid</creator><creator>Javadirad, Seyed Morteza</creator><general>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</general><general>Knowledge E</general><general>Shahid Sadoughi University of Medical Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20201101</creationdate><title>Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study</title><author>Norioun, Hamid ; Motovali-Bashi, Majid ; Javadirad, Seyed Morteza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-c67368170d342e9442aa895c1bd49934ec72cdbd0dcdcb559a29649c3e44825c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>hsa-mir-27a-3p, male infertility, kdm3a</topic><topic>Infertility</topic><topic>Male reproductive system</topic><topic>Mens health</topic><topic>Spermatogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Norioun, Hamid</creatorcontrib><creatorcontrib>Motovali-Bashi, Majid</creatorcontrib><creatorcontrib>Javadirad, Seyed Morteza</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International Journal of Reproductive BioMedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Norioun, Hamid</au><au>Motovali-Bashi, Majid</au><au>Javadirad, Seyed Morteza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study</atitle><jtitle>International Journal of Reproductive BioMedicine</jtitle><addtitle>Int J Reprod Biomed</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>18</volume><issue>11</issue><spage>961</spage><epage>968</epage><pages>961-968</pages><issn>2476-4108</issn><eissn>2476-3772</eissn><abstract>The role of
and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of
is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility.
The aim of this study was to evaluate the expression level of
in azoospermic men to reveal its possible association with infertility.
This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and
was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RT-qPCR) method was used and statistical analysis was performed to compare the expression level of
in both OA and NOA individuals.
In silico analysis suggested
, with its potential binding ability to target
transcripts. The expression analysis of candidate
indicated its significant overexpression in NOA men.
The
was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly
and indirectly
and
. Therefore, spermatogenesis could be misled and male infertility could be developed.</abstract><cop>Iran</cop><pub>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</pub><pmid>33349804</pmid><doi>10.18502/ijrm.v13i11.7963</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | hsa-mir-27a-3p, male infertility, kdm3a Infertility Male reproductive system Mens health Spermatogenesis |
| title | Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study |
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