Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells
With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth's magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been we...
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| Veröffentlicht in: | International journal of molecular sciences 2020-12, Vol.21 (23), p.9304 |
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| creator | Xue, Xunwen Ali, Yasser F Liu, Caorui Hong, Zhiqiang Luo, Wanrong Nie, Jing Li, Bingyan Jiao, Yang Liu, Ning-Ang |
| description | With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth's magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF ( |
| doi_str_mv | 10.3390/ijms21239304 |
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However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21239304</identifier><identifier>PMID: 33291307</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Apoptosis ; Astronauts ; Biological effects ; Brief Report ; Bronchi - cytology ; Cell culture ; Cell cycle ; Cell Line ; Deoxyribonucleic acid ; DNA ; DNA Breaks, Double-Stranded ; DNA damage ; DNA Repair ; Earth ; Epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - radiation effects ; Genotoxicity ; Geomagnetic field ; Geomagnetism ; Histones - metabolism ; Humans ; Ionizing radiation ; Magnetic Fields ; Magnetic shielding ; Radiation shielding ; Radiation Tolerance ; Radiation, Ionizing ; Repair</subject><ispartof>International journal of molecular sciences, 2020-12, Vol.21 (23), p.9304</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-921683d42005f8c56a1c1eb485a27f0ec2e8da424346ed2f508e99df5a2df41e3</citedby><cites>FETCH-LOGICAL-c412t-921683d42005f8c56a1c1eb485a27f0ec2e8da424346ed2f508e99df5a2df41e3</cites><orcidid>0000-0001-7053-0945</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730591/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33291307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Xunwen</creatorcontrib><creatorcontrib>Ali, Yasser F</creatorcontrib><creatorcontrib>Liu, Caorui</creatorcontrib><creatorcontrib>Hong, Zhiqiang</creatorcontrib><creatorcontrib>Luo, Wanrong</creatorcontrib><creatorcontrib>Nie, Jing</creatorcontrib><creatorcontrib>Li, Bingyan</creatorcontrib><creatorcontrib>Jiao, Yang</creatorcontrib><creatorcontrib>Liu, Ning-Ang</creatorcontrib><title>Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>With the advent of long-duration space explorations, ionizing radiation (IR) may pose a constant threat to astronauts without the protection of Earth's magnetic field, or hypomagnetic field (HMF). However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR.</description><subject>Apoptosis</subject><subject>Astronauts</subject><subject>Biological effects</subject><subject>Brief Report</subject><subject>Bronchi - cytology</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Line</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Breaks, Double-Stranded</subject><subject>DNA damage</subject><subject>DNA Repair</subject><subject>Earth</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - radiation effects</subject><subject>Genotoxicity</subject><subject>Geomagnetic field</subject><subject>Geomagnetism</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Ionizing radiation</subject><subject>Magnetic Fields</subject><subject>Magnetic shielding</subject><subject>Radiation shielding</subject><subject>Radiation Tolerance</subject><subject>Radiation, Ionizing</subject><subject>Repair</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1PGzEQxS1EBWnKjTOyxIVDU_y1XxckGtJQKYKKlrPleGezjnbt1N4F5b-vV6FR4DSjeb8Z-fkhdE7JN84Lcm3WbWCU8YITcYRGVDA2ISTNjg_6U_Q5hDUhjLOkOEGnnLOCcpKN0OscXKtWFjqj8e_aQFMau8IzWyurIeAnVRrVGWfxEwQTumGKl1v8y7vWdQN693AbtY0yfhjGnYCNxfd9qyz-7p3VtVENnm1MV0MztFNomvAFfapUE-DsrY7R84_Zn-n9ZPE4_zm9XUy0oKybFIymOS8FIySpcp2kimoKS5EnimUVAc0gL5VggosUSlYlJIeiKKsol5WgwMfoZnd30y9bKDXYzqtGbrxpld9Kp4x8r1hTy5V7kVnGSRI_aYyu3g5497eH0MnWBB0tKAuuD5KJNE8zwrM8opcf0LXrvY32BqqIJmguIvV1R2nvQvBQ7R9DiRwSlYeJRvzi0MAe_h8h_welOZ2t</recordid><startdate>20201206</startdate><enddate>20201206</enddate><creator>Xue, Xunwen</creator><creator>Ali, Yasser F</creator><creator>Liu, Caorui</creator><creator>Hong, Zhiqiang</creator><creator>Luo, Wanrong</creator><creator>Nie, Jing</creator><creator>Li, Bingyan</creator><creator>Jiao, Yang</creator><creator>Liu, Ning-Ang</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7053-0945</orcidid></search><sort><creationdate>20201206</creationdate><title>Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells</title><author>Xue, Xunwen ; 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However, the potential biological effects of a HMF on the cellular response to IR have not been well characterized so far. In this study, immortalized human bronchial epithelial cells were exposed to X-rays under either a geomagnetic field (GMF, ~50 uT) or HMF (<50 nT) culture condition. A significant increase of the cell survival rate in HMF after radiation was observed by colony formation analysis. The kinetics of DNA double-strand breaks (DSBs), determined by γH2AX foci formation and disappearance, presented a faster decrease of foci-positive cells and a significantly lower mean number of γH2AX foci per nucleus in HMF-cultured cells than in GMF-cultured cells after radiation. In addition, a γH2AX/53BP1 colocalization assay showed an upregulated DSB recovery rate in HMF cultured cells. These findings provided the first evidence that HMF exposure may enhance the cellular DSB repair efficiency upon radiation, and consequently modulate the genotoxic effects of IR.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33291307</pmid><doi>10.3390/ijms21239304</doi><orcidid>https://orcid.org/0000-0001-7053-0945</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Apoptosis Astronauts Biological effects Brief Report Bronchi - cytology Cell culture Cell cycle Cell Line Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA damage DNA Repair Earth Epithelial cells Epithelial Cells - metabolism Epithelial Cells - radiation effects Genotoxicity Geomagnetic field Geomagnetism Histones - metabolism Humans Ionizing radiation Magnetic Fields Magnetic shielding Radiation shielding Radiation Tolerance Radiation, Ionizing Repair |
| title | Geomagnetic Shielding Enhances Radiation Resistance by Promoting DNA Repair Process in Human Bronchial Epithelial Cells |
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