Smooth Muscle Cell Responses to Poly(ε-Caprolactone) Triacrylate Networks with Different Crosslinking Time
Poly(ε-caprolactone) triacrylate (PCLTA) is attractive in tissue engineering because of its good biocompatibility and processability. The crosslinking time strongly influences PCLTAs cellular behaviors. To investigate these influences, PCLTAs with different molecular weights were crosslinked under U...
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| Veröffentlicht in: | International journal of molecular sciences 2020-11, Vol.21 (23), p.8932 |
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| creator | Wang, Jing Liu, Li Wang, Aoning Liu, Xiang Zhang, Yi Wang, Zhoulu Dou, Jinbo |
| description | Poly(ε-caprolactone) triacrylate (PCLTA) is attractive in tissue engineering because of its good biocompatibility and processability. The crosslinking time strongly influences PCLTAs cellular behaviors. To investigate these influences, PCLTAs with different molecular weights were crosslinked under UV light for times ranging from 1 to 20 min. The crosslinking efficiency of PCLTA increased with decreasing the molecular weight and increasing crosslinking time which could increase the gel fraction and network stiffness and decrease the swelling ratio. Then, the PCLTA networks crosslinked for different time were used as substrates for culturing rat aortic smooth muscle cells (SMCs). SMC attachment and proliferation all increased when the PCLTA molecular weight increased from 8k to 10k and then to 20k at the same crosslinking time. For the same PCLTA, SMC attachment, proliferation, and focal adhesions increased with increasing the crosslinking time, in particular, between the substrates crosslinked for less than 3 min and longer than 5 min. This work will provide a good experimental basis for the application of PCLTA. |
| doi_str_mv | 10.3390/ijms21238932 |
| format | Article |
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The crosslinking time strongly influences PCLTAs cellular behaviors. To investigate these influences, PCLTAs with different molecular weights were crosslinked under UV light for times ranging from 1 to 20 min. The crosslinking efficiency of PCLTA increased with decreasing the molecular weight and increasing crosslinking time which could increase the gel fraction and network stiffness and decrease the swelling ratio. Then, the PCLTA networks crosslinked for different time were used as substrates for culturing rat aortic smooth muscle cells (SMCs). SMC attachment and proliferation all increased when the PCLTA molecular weight increased from 8k to 10k and then to 20k at the same crosslinking time. For the same PCLTA, SMC attachment, proliferation, and focal adhesions increased with increasing the crosslinking time, in particular, between the substrates crosslinked for less than 3 min and longer than 5 min. This work will provide a good experimental basis for the application of PCLTA.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21238932</identifier><identifier>PMID: 33255621</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acrylates - chemistry ; Animals ; Aorta ; Aorta - drug effects ; Aorta - growth & development ; Biocompatibility ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Cell adhesion & migration ; Cell Adhesion - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cross-Linking Reagents - chemistry ; Cross-Linking Reagents - pharmacology ; Crosslinking ; Efficiency ; Humans ; Mechanical properties ; Molecular weight ; Muscles ; Myocytes, Smooth Muscle - drug effects ; Myocytes, Smooth Muscle - metabolism ; Organ Culture Techniques ; Physical properties ; Polycaprolactone ; Polyesters - chemistry ; Polyesters - pharmacology ; Polymers ; Rats ; Smooth muscle ; Stiffness ; Surface Properties ; Swelling ratio ; Tissue Engineering ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>International journal of molecular sciences, 2020-11, Vol.21 (23), p.8932</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c369t-20bd5caaad570d360eef735d81d0546707212d95a7bbd8a60b70c5fc75b823ca3</cites><orcidid>0000-0002-1603-8623</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728059/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728059/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33255621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Wang, Aoning</creatorcontrib><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Wang, Zhoulu</creatorcontrib><creatorcontrib>Dou, Jinbo</creatorcontrib><title>Smooth Muscle Cell Responses to Poly(ε-Caprolactone) Triacrylate Networks with Different Crosslinking Time</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Poly(ε-caprolactone) triacrylate (PCLTA) is attractive in tissue engineering because of its good biocompatibility and processability. The crosslinking time strongly influences PCLTAs cellular behaviors. To investigate these influences, PCLTAs with different molecular weights were crosslinked under UV light for times ranging from 1 to 20 min. The crosslinking efficiency of PCLTA increased with decreasing the molecular weight and increasing crosslinking time which could increase the gel fraction and network stiffness and decrease the swelling ratio. Then, the PCLTA networks crosslinked for different time were used as substrates for culturing rat aortic smooth muscle cells (SMCs). SMC attachment and proliferation all increased when the PCLTA molecular weight increased from 8k to 10k and then to 20k at the same crosslinking time. For the same PCLTA, SMC attachment, proliferation, and focal adhesions increased with increasing the crosslinking time, in particular, between the substrates crosslinked for less than 3 min and longer than 5 min. This work will provide a good experimental basis for the application of PCLTA.</description><subject>Acrylates - chemistry</subject><subject>Animals</subject><subject>Aorta</subject><subject>Aorta - drug effects</subject><subject>Aorta - growth & development</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Cross-Linking Reagents - pharmacology</subject><subject>Crosslinking</subject><subject>Efficiency</subject><subject>Humans</subject><subject>Mechanical properties</subject><subject>Molecular weight</subject><subject>Muscles</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Organ Culture Techniques</subject><subject>Physical properties</subject><subject>Polycaprolactone</subject><subject>Polyesters - chemistry</subject><subject>Polyesters - pharmacology</subject><subject>Polymers</subject><subject>Rats</subject><subject>Smooth muscle</subject><subject>Stiffness</subject><subject>Surface Properties</subject><subject>Swelling ratio</subject><subject>Tissue Engineering</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkd1u1DAQhS0Eou3CHdfIEjdFIjCx13ZyUwmFX6n8CJZry3EmrXedeLGTVvtgvAbPhFct1cLVjDSfzsyZQ8iTEl5yXsMrtx4SKxmvas7ukeNyyVgBINX9g_6InKS0BmCcifohOeK5CsnKY7L5PoQwXdJPc7IeaYPe02-YtmFMmOgU6Nfgd6e_fxWN2cbgjZ3CiM_pKjpj486bCelnnK5D3CR67bLQG9f3GHGcaBNDSt6NGzde0JUb8BF50Buf8PFtXZAf796umg_F-Zf3H5vX54Xlsp4KBm0nrDGmEwo6LgGxV1x0VdmBWEoFKtvtamFU23aVkdAqsKK3SrQV49bwBTm70d3O7YCdzcdE4_U2usHEnQ7G6X8no7vUF-FKK8UqEHUWOL0ViOHnjGnSg0s2v8aMGOak2VJK4FLmdQvy7D90HeY4Znt7SghVAhOZenFD2f1PIvZ3x5Sg9ynqwxQz_vTQwB38Nzb-B-A5mwo</recordid><startdate>20201125</startdate><enddate>20201125</enddate><creator>Wang, Jing</creator><creator>Liu, Li</creator><creator>Wang, Aoning</creator><creator>Liu, Xiang</creator><creator>Zhang, Yi</creator><creator>Wang, Zhoulu</creator><creator>Dou, Jinbo</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1603-8623</orcidid></search><sort><creationdate>20201125</creationdate><title>Smooth Muscle Cell Responses to Poly(ε-Caprolactone) Triacrylate Networks with Different Crosslinking Time</title><author>Wang, Jing ; Liu, Li ; Wang, Aoning ; Liu, Xiang ; Zhang, Yi ; Wang, Zhoulu ; Dou, Jinbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-20bd5caaad570d360eef735d81d0546707212d95a7bbd8a60b70c5fc75b823ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acrylates - chemistry</topic><topic>Animals</topic><topic>Aorta</topic><topic>Aorta - drug effects</topic><topic>Aorta - growth & development</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Cell adhesion & migration</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Cross-Linking Reagents - pharmacology</topic><topic>Crosslinking</topic><topic>Efficiency</topic><topic>Humans</topic><topic>Mechanical properties</topic><topic>Molecular weight</topic><topic>Muscles</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Organ Culture Techniques</topic><topic>Physical properties</topic><topic>Polycaprolactone</topic><topic>Polyesters - chemistry</topic><topic>Polyesters - pharmacology</topic><topic>Polymers</topic><topic>Rats</topic><topic>Smooth muscle</topic><topic>Stiffness</topic><topic>Surface Properties</topic><topic>Swelling ratio</topic><topic>Tissue Engineering</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Wang, Aoning</creatorcontrib><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Wang, Zhoulu</creatorcontrib><creatorcontrib>Dou, Jinbo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jing</au><au>Liu, Li</au><au>Wang, Aoning</au><au>Liu, Xiang</au><au>Zhang, Yi</au><au>Wang, Zhoulu</au><au>Dou, Jinbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Smooth Muscle Cell Responses to Poly(ε-Caprolactone) Triacrylate Networks with Different Crosslinking Time</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-11-25</date><risdate>2020</risdate><volume>21</volume><issue>23</issue><spage>8932</spage><pages>8932-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Poly(ε-caprolactone) triacrylate (PCLTA) is attractive in tissue engineering because of its good biocompatibility and processability. The crosslinking time strongly influences PCLTAs cellular behaviors. To investigate these influences, PCLTAs with different molecular weights were crosslinked under UV light for times ranging from 1 to 20 min. The crosslinking efficiency of PCLTA increased with decreasing the molecular weight and increasing crosslinking time which could increase the gel fraction and network stiffness and decrease the swelling ratio. Then, the PCLTA networks crosslinked for different time were used as substrates for culturing rat aortic smooth muscle cells (SMCs). SMC attachment and proliferation all increased when the PCLTA molecular weight increased from 8k to 10k and then to 20k at the same crosslinking time. For the same PCLTA, SMC attachment, proliferation, and focal adhesions increased with increasing the crosslinking time, in particular, between the substrates crosslinked for less than 3 min and longer than 5 min. This work will provide a good experimental basis for the application of PCLTA.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33255621</pmid><doi>10.3390/ijms21238932</doi><orcidid>https://orcid.org/0000-0002-1603-8623</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acrylates - chemistry Animals Aorta Aorta - drug effects Aorta - growth & development Biocompatibility Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Cell adhesion & migration Cell Adhesion - drug effects Cell proliferation Cell Proliferation - drug effects Cross-Linking Reagents - chemistry Cross-Linking Reagents - pharmacology Crosslinking Efficiency Humans Mechanical properties Molecular weight Muscles Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism Organ Culture Techniques Physical properties Polycaprolactone Polyesters - chemistry Polyesters - pharmacology Polymers Rats Smooth muscle Stiffness Surface Properties Swelling ratio Tissue Engineering Ultraviolet radiation Ultraviolet Rays |
| title | Smooth Muscle Cell Responses to Poly(ε-Caprolactone) Triacrylate Networks with Different Crosslinking Time |
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