Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study
Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell‐derived cardiomyocytes (hiPS‐CMs) can restore contractile function. However, cells within...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2020-10, Vol.108 (10), p.2123-2132 |
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| creator | Robbins, Emily R. Pins, George D. Laflamme, Michael A. Gaudette, Glenn R. |
| description | Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell‐derived cardiomyocytes (hiPS‐CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS‐CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS‐CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21 days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS‐CMs adhered to coated and noncoated decellularized leaves for 21 days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25 ± 0.39% vs. 1.54 ± 0.60%; p |
| doi_str_mv | 10.1002/jbm.a.36971 |
| format | Article |
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Tissue engineered cardiac patches containing human induced pluripotent stem cell‐derived cardiomyocytes (hiPS‐CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS‐CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS‐CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21 days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS‐CMs adhered to coated and noncoated decellularized leaves for 21 days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25 ± 0.39% vs. 1.54 ± 0.60%; p < .05). However, no differences in contraction were found between coated leaves, coated tissue culture plastic (TCP), noncoated leaves, or noncoated TCP at other time points. No significant differences were observed in hiPS‐CM spreading or sarcomere lengths on leaves with or without coating. This study demonstrates that cardiac scaffolds can be created from decellularized leaves without ECM coatings. Noncoated decellularized leaf surfaces facilitate robust cell attachment for an engineered tissue patch.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.36971</identifier><identifier>PMID: 32323417</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Biomaterials ; Biomedical materials ; Blood flow ; Cardiomyocytes ; Cell adhesion ; Cell culture ; Coatings ; Collagen ; Collagen (type IV) ; Congestive heart failure ; Contraction ; decellularized leaves ; Extracellular matrix ; Fibronectin ; high‐speed imaging ; Immunofluorescence ; Leaves ; mechanical contraction ; Muscle contraction ; Myocardial infarction ; Pluripotency ; pluripotent stem cell‐derived cardiomyocytes ; Proteins ; Recovery of function ; Scaffolds ; Spinach ; Stem cells ; Tissue culture</subject><ispartof>Journal of biomedical materials research. Part A, 2020-10, Vol.108 (10), p.2123-2132</ispartof><rights>2020 Wiley Periodicals, Inc.</rights><rights>2020 Wiley Periodicals LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4891-b5a3597dad841f2ea65a6693a241f99d395bb29f49bcbdc169ac1fc26053af1a3</citedby><cites>FETCH-LOGICAL-c4891-b5a3597dad841f2ea65a6693a241f99d395bb29f49bcbdc169ac1fc26053af1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.36971$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.36971$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32323417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robbins, Emily R.</creatorcontrib><creatorcontrib>Pins, George D.</creatorcontrib><creatorcontrib>Laflamme, Michael A.</creatorcontrib><creatorcontrib>Gaudette, Glenn R.</creatorcontrib><title>Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study</title><title>Journal of biomedical materials research. Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell‐derived cardiomyocytes (hiPS‐CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS‐CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS‐CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21 days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS‐CMs adhered to coated and noncoated decellularized leaves for 21 days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25 ± 0.39% vs. 1.54 ± 0.60%; p < .05). However, no differences in contraction were found between coated leaves, coated tissue culture plastic (TCP), noncoated leaves, or noncoated TCP at other time points. No significant differences were observed in hiPS‐CM spreading or sarcomere lengths on leaves with or without coating. This study demonstrates that cardiac scaffolds can be created from decellularized leaves without ECM coatings. Noncoated decellularized leaf surfaces facilitate robust cell attachment for an engineered tissue patch.</description><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Blood flow</subject><subject>Cardiomyocytes</subject><subject>Cell adhesion</subject><subject>Cell culture</subject><subject>Coatings</subject><subject>Collagen</subject><subject>Collagen (type IV)</subject><subject>Congestive heart failure</subject><subject>Contraction</subject><subject>decellularized leaves</subject><subject>Extracellular matrix</subject><subject>Fibronectin</subject><subject>high‐speed imaging</subject><subject>Immunofluorescence</subject><subject>Leaves</subject><subject>mechanical contraction</subject><subject>Muscle contraction</subject><subject>Myocardial infarction</subject><subject>Pluripotency</subject><subject>pluripotent stem cell‐derived cardiomyocytes</subject><subject>Proteins</subject><subject>Recovery of function</subject><subject>Scaffolds</subject><subject>Spinach</subject><subject>Stem cells</subject><subject>Tissue culture</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kcuLFDEQh4Mo7kNP3iXgRZAeO8-eeFgYh_XFLl70HKrTyU6G7s6YpHcY__pNO-uiHiSHpKiPj6r8EHpB6gWpa_p22w4LWDCpGvIInRIhaMWVFI_nN1cVo0qeoLOUtgWWtaBP0Qmj5XDSnKL9OlrIPow4OAzYhDFHMNn3Frc-DJBt9NBjF8NQ2p01tu-nHqL_aTucdn4Es8G9BYf3Pm_ClPHl-hrvYsjWj0VX3OPNO7wacSlvfY4Bpzx1h2foiYM-2ef39zn6_uHy2_pTdfX14-f16qoyfKlI1QpgQjUddEtOHLUgBUipGNBSKtUxJdqWKsdVa9rOEKnAEGfmPRk4AuwcXRy9u6kdbGfsvF-vd9EPEA86gNd_d0a_0TfhVjcNFUzIInh9L4jhx2RT1oNP8y_AaMOUNGWKU7FsOCnoq3_QbZjiWNbTlDPaLAlTTaHeHCkTQ0rRuodhSK3nQHUJVIP-FWihX_45_wP7O8EC0COwL5kd_ufSX95fr47WOxu4rf8</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Robbins, Emily R.</creator><creator>Pins, George D.</creator><creator>Laflamme, Michael A.</creator><creator>Gaudette, Glenn R.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>202010</creationdate><title>Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study</title><author>Robbins, Emily R. ; Pins, George D. ; Laflamme, Michael A. ; Gaudette, Glenn R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4891-b5a3597dad841f2ea65a6693a241f99d395bb29f49bcbdc169ac1fc26053af1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Blood flow</topic><topic>Cardiomyocytes</topic><topic>Cell adhesion</topic><topic>Cell culture</topic><topic>Coatings</topic><topic>Collagen</topic><topic>Collagen (type IV)</topic><topic>Congestive heart failure</topic><topic>Contraction</topic><topic>decellularized leaves</topic><topic>Extracellular matrix</topic><topic>Fibronectin</topic><topic>high‐speed imaging</topic><topic>Immunofluorescence</topic><topic>Leaves</topic><topic>mechanical contraction</topic><topic>Muscle contraction</topic><topic>Myocardial infarction</topic><topic>Pluripotency</topic><topic>pluripotent stem cell‐derived cardiomyocytes</topic><topic>Proteins</topic><topic>Recovery of function</topic><topic>Scaffolds</topic><topic>Spinach</topic><topic>Stem cells</topic><topic>Tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robbins, Emily R.</creatorcontrib><creatorcontrib>Pins, George D.</creatorcontrib><creatorcontrib>Laflamme, Michael A.</creatorcontrib><creatorcontrib>Gaudette, Glenn R.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robbins, Emily R.</au><au>Pins, George D.</au><au>Laflamme, Michael A.</au><au>Gaudette, Glenn R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2020-10</date><risdate>2020</risdate><volume>108</volume><issue>10</issue><spage>2123</spage><epage>2132</epage><pages>2123-2132</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Myocardial infarction (MI) results in the death of cardiac tissue, decreases regional contraction, and can lead to heart failure. Tissue engineered cardiac patches containing human induced pluripotent stem cell‐derived cardiomyocytes (hiPS‐CMs) can restore contractile function. However, cells within thick patches require vasculature for blood flow. Recently, we demonstrated fibronectin coated decellularized leaves provide a suitable scaffold for hiPS‐CMs. Yet, the necessity of this additional coating step is unclear. Therefore, we compared hiPS‐CM behavior on decellularized leaves coated with collagen IV or fibronectin extracellular matrix (ECM) proteins to noncoated leaves for up to 21 days. Successful coating was verified by immunofluorescence. Similar numbers of hiPS‐CMs adhered to coated and noncoated decellularized leaves for 21 days. At Day 14, collagen IV coated leaves contracted more than noncoated leaves (3.25 ± 0.39% vs. 1.54 ± 0.60%; p < .05). However, no differences in contraction were found between coated leaves, coated tissue culture plastic (TCP), noncoated leaves, or noncoated TCP at other time points. No significant differences were observed in hiPS‐CM spreading or sarcomere lengths on leaves with or without coating. This study demonstrates that cardiac scaffolds can be created from decellularized leaves without ECM coatings. Noncoated decellularized leaf surfaces facilitate robust cell attachment for an engineered tissue patch.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>32323417</pmid><doi>10.1002/jbm.a.36971</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley-Blackwell Journals |
| subjects | Biomaterials Biomedical materials Blood flow Cardiomyocytes Cell adhesion Cell culture Coatings Collagen Collagen (type IV) Congestive heart failure Contraction decellularized leaves Extracellular matrix Fibronectin high‐speed imaging Immunofluorescence Leaves mechanical contraction Muscle contraction Myocardial infarction Pluripotency pluripotent stem cell‐derived cardiomyocytes Proteins Recovery of function Scaffolds Spinach Stem cells Tissue culture |
| title | Creation of a contractile biomaterial from a decellularized spinach leaf without ECM protein coating: An in vitro study |
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