Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2
The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the...
Gespeichert in:
| Veröffentlicht in: | Experimental and therapeutic medicine 2021-01, Vol.21 (1), p.64-64 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 64 |
|---|---|
| container_issue | 1 |
| container_start_page | 64 |
| container_title | Experimental and therapeutic medicine |
| container_volume | 21 |
| creator | Liang, Ying Kang, Liying Qi, Zihe Gao, Xing Quan, Huili |
| description | The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H2O2-induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit-8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor-α (TNF-α) were measured. Western blotting was performed to detect the protein expression of cleaved caspase-3 and caspase-9. Furthermore, immunocytochemistry was used to detect the expression of TNF-α. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H2O2-induced increased MDA levels, and reversed the H2O2-induced suppression of SOD and GSH activities in KGN cells (P |
| doi_str_mv | 10.3892/etm.2020.9496 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7716637</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2470576314</sourcerecordid><originalsourceid>FETCH-LOGICAL-p240t-e48df3d95b9a410f27cf07b6b3c8671acbf01666d625dc22e4c86901ae1666313</originalsourceid><addsrcrecordid>eNpdj81rGzEQxUVpqYPrY-6CXnKxq49daXUpFNMkhUAObc7LrKS1ZfThrlYG96-vTHxJ5jIzb3485iF0S8mGd4p9s3PYMMLIRjVKfEA3VCq2poS2H68zUR1doFXOB1KrFbTr2s9owTkXLRHNDfK_wZ8c4OD87NI07d0_wDn5UreIIRrs5oxBz-5ksU7hmEo0VQjWVxxmi_clQMS7CWLxKQPW1ntsIMDOYhdN0dbg4Ywf2TP7gj6N4LNdXfsSvdz__LN9XD89P_za_nhaH1lD5rVtOjNyo9pBQUPJyKQeiRzEwHUnJAU9jIQKIYxgrdGM2abqilCwF5VTvkTfX32PZQjWaBvnCXx_nFyA6dwncP3bS3T7fpdOvZTVgctqcHc1mNLfYvPcB5cvwSDaVHLPGsm56hTvKvr1HXpIZYo13oUirawPNfw_AyKCwg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2470576314</pqid></control><display><type>article</type><title>Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2</title><source>PubMed Central</source><creator>Liang, Ying ; Kang, Liying ; Qi, Zihe ; Gao, Xing ; Quan, Huili</creator><creatorcontrib>Liang, Ying ; Kang, Liying ; Qi, Zihe ; Gao, Xing ; Quan, Huili</creatorcontrib><description>The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H2O2-induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit-8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor-α (TNF-α) were measured. Western blotting was performed to detect the protein expression of cleaved caspase-3 and caspase-9. Furthermore, immunocytochemistry was used to detect the expression of TNF-α. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H2O2-induced increased MDA levels, and reversed the H2O2-induced suppression of SOD and GSH activities in KGN cells (P<0.05). Treatment with Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B significantly reduced the overexpression of cleaved caspase-3, cleaved caspase-9 and TNF-α compared with the H2O2-treated group (P<0.05). Therefore, the present results indicated that Salvia miltiorrhiza solution and its main water-soluble compounds, salvianic acid A and salvianolic acid B, ameliorated KGN cell damage induced by H2O2.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2020.9496</identifier><identifier>PMID: 33365064</identifier><language>eng</language><publisher>Athens: Spandidos Publications UK Ltd</publisher><subject>Antibodies ; Biotechnology ; Cell culture ; Embryos ; Engineering ; Infertility ; Lipid peroxidation ; Medical research ; Ovaries ; Ovulation ; Oxidative stress ; Polycystic ovary syndrome ; Proteins ; Reproductive technologies ; Tumor necrosis factor-TNF</subject><ispartof>Experimental and therapeutic medicine, 2021-01, Vol.21 (1), p.64-64</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2021</rights><rights>Copyright: © Liang et al. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716637/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716637/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids></links><search><creatorcontrib>Liang, Ying</creatorcontrib><creatorcontrib>Kang, Liying</creatorcontrib><creatorcontrib>Qi, Zihe</creatorcontrib><creatorcontrib>Gao, Xing</creatorcontrib><creatorcontrib>Quan, Huili</creatorcontrib><title>Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2</title><title>Experimental and therapeutic medicine</title><description>The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H2O2-induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit-8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor-α (TNF-α) were measured. Western blotting was performed to detect the protein expression of cleaved caspase-3 and caspase-9. Furthermore, immunocytochemistry was used to detect the expression of TNF-α. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H2O2-induced increased MDA levels, and reversed the H2O2-induced suppression of SOD and GSH activities in KGN cells (P<0.05). Treatment with Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B significantly reduced the overexpression of cleaved caspase-3, cleaved caspase-9 and TNF-α compared with the H2O2-treated group (P<0.05). Therefore, the present results indicated that Salvia miltiorrhiza solution and its main water-soluble compounds, salvianic acid A and salvianolic acid B, ameliorated KGN cell damage induced by H2O2.</description><subject>Antibodies</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Embryos</subject><subject>Engineering</subject><subject>Infertility</subject><subject>Lipid peroxidation</subject><subject>Medical research</subject><subject>Ovaries</subject><subject>Ovulation</subject><subject>Oxidative stress</subject><subject>Polycystic ovary syndrome</subject><subject>Proteins</subject><subject>Reproductive technologies</subject><subject>Tumor necrosis factor-TNF</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdj81rGzEQxUVpqYPrY-6CXnKxq49daXUpFNMkhUAObc7LrKS1ZfThrlYG96-vTHxJ5jIzb3485iF0S8mGd4p9s3PYMMLIRjVKfEA3VCq2poS2H68zUR1doFXOB1KrFbTr2s9owTkXLRHNDfK_wZ8c4OD87NI07d0_wDn5UreIIRrs5oxBz-5ksU7hmEo0VQjWVxxmi_clQMS7CWLxKQPW1ntsIMDOYhdN0dbg4Ywf2TP7gj6N4LNdXfsSvdz__LN9XD89P_za_nhaH1lD5rVtOjNyo9pBQUPJyKQeiRzEwHUnJAU9jIQKIYxgrdGM2abqilCwF5VTvkTfX32PZQjWaBvnCXx_nFyA6dwncP3bS3T7fpdOvZTVgctqcHc1mNLfYvPcB5cvwSDaVHLPGsm56hTvKvr1HXpIZYo13oUirawPNfw_AyKCwg</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Liang, Ying</creator><creator>Kang, Liying</creator><creator>Qi, Zihe</creator><creator>Gao, Xing</creator><creator>Quan, Huili</creator><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210101</creationdate><title>Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2</title><author>Liang, Ying ; Kang, Liying ; Qi, Zihe ; Gao, Xing ; Quan, Huili</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-e48df3d95b9a410f27cf07b6b3c8671acbf01666d625dc22e4c86901ae1666313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Embryos</topic><topic>Engineering</topic><topic>Infertility</topic><topic>Lipid peroxidation</topic><topic>Medical research</topic><topic>Ovaries</topic><topic>Ovulation</topic><topic>Oxidative stress</topic><topic>Polycystic ovary syndrome</topic><topic>Proteins</topic><topic>Reproductive technologies</topic><topic>Tumor necrosis factor-TNF</topic><toplevel>online_resources</toplevel><creatorcontrib>Liang, Ying</creatorcontrib><creatorcontrib>Kang, Liying</creatorcontrib><creatorcontrib>Qi, Zihe</creatorcontrib><creatorcontrib>Gao, Xing</creatorcontrib><creatorcontrib>Quan, Huili</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Ying</au><au>Kang, Liying</au><au>Qi, Zihe</au><au>Gao, Xing</au><au>Quan, Huili</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2</atitle><jtitle>Experimental and therapeutic medicine</jtitle><date>2021-01-01</date><risdate>2021</risdate><volume>21</volume><issue>1</issue><spage>64</spage><epage>64</epage><pages>64-64</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H2O2-induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit-8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor-α (TNF-α) were measured. Western blotting was performed to detect the protein expression of cleaved caspase-3 and caspase-9. Furthermore, immunocytochemistry was used to detect the expression of TNF-α. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H2O2-induced increased MDA levels, and reversed the H2O2-induced suppression of SOD and GSH activities in KGN cells (P<0.05). Treatment with Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B significantly reduced the overexpression of cleaved caspase-3, cleaved caspase-9 and TNF-α compared with the H2O2-treated group (P<0.05). Therefore, the present results indicated that Salvia miltiorrhiza solution and its main water-soluble compounds, salvianic acid A and salvianolic acid B, ameliorated KGN cell damage induced by H2O2.</abstract><cop>Athens</cop><pub>Spandidos Publications UK Ltd</pub><pmid>33365064</pmid><doi>10.3892/etm.2020.9496</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-0981 |
| ispartof | Experimental and therapeutic medicine, 2021-01, Vol.21 (1), p.64-64 |
| issn | 1792-0981 1792-1015 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7716637 |
| source | PubMed Central |
| subjects | Antibodies Biotechnology Cell culture Embryos Engineering Infertility Lipid peroxidation Medical research Ovaries Ovulation Oxidative stress Polycystic ovary syndrome Proteins Reproductive technologies Tumor necrosis factor-TNF |
| title | Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T10%3A13%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Salvia%20miltiorrhiza%20solution%20and%20its%20active%20compounds%20ameliorate%20human%20granulosa%20cell%20damage%20induced%20by%20H2O2&rft.jtitle=Experimental%20and%20therapeutic%20medicine&rft.au=Liang,%20Ying&rft.date=2021-01-01&rft.volume=21&rft.issue=1&rft.spage=64&rft.epage=64&rft.pages=64-64&rft.issn=1792-0981&rft.eissn=1792-1015&rft_id=info:doi/10.3892/etm.2020.9496&rft_dat=%3Cproquest_pubme%3E2470576314%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2470576314&rft_id=info:pmid/33365064&rfr_iscdi=true |