Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress
Vascular endothelial dysfunction is an essential and early sign of diabetic macroangiopathy, a primary complication of diabetes mellitus. Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-12 |
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| creator | Li, Xiaoli Wang, Zichen Zhang, Xinying Li, Li Zhu, Ranran Chen, Yumin Li, Hui Yin, Yonghui Zhou, Ji |
| description | Vascular endothelial dysfunction is an essential and early sign of diabetic macroangiopathy, a primary complication of diabetes mellitus. Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate the effect of the granule form of the extract produced from the dried root of Astragalus membranaceous (AM) combination with the granule form of the extract produced from the dried Angelica sinensis (AS) on diabetic macroangiopathy and its underlying mechanism. Herein, rats were treated by AM-AS at a ratio of 3 : 2 via intragastric administration. High glucose-induced human umbilical vein vascular endothelial cells (HUVECs) were then treated with drug-containing serum collected from the rats. In high glucose-treated HUVECs, AM-AS combination increased cell viability (P |
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Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate the effect of the granule form of the extract produced from the dried root of Astragalus membranaceous (AM) combination with the granule form of the extract produced from the dried Angelica sinensis (AS) on diabetic macroangiopathy and its underlying mechanism. Herein, rats were treated by AM-AS at a ratio of 3 : 2 via intragastric administration. High glucose-induced human umbilical vein vascular endothelial cells (HUVECs) were then treated with drug-containing serum collected from the rats. In high glucose-treated HUVECs, AM-AS combination increased cell viability (P<0.05), decreased the percentage of apoptotic cells (P<0.05) and the expression of the proapoptosis protein caspase 3 (P<0.05), reduced the proportion of cells in the G0/G1 phase (P<0.05), decreased reactive oxygen species level (P<0.05), enhanced cell migration and invasion (P<0.05), and reduced the level of 8-iso-prostaglandin F2alpha. These results indicate that AM-AS combination at the ratio of 3 : 2 ameliorated HUVEC dysfunction by regulating apoptosis, cell migration, and invasion, which might be mediated by their regulatory effect on reactive oxygen species production. The current study provides a theoretical basis for the treatment of diabetic macroangiopathy using AM-AS.]]></description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/6031782</identifier><identifier>PMID: 33293989</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Angelica sinensis ; Apoptosis ; Astragalus membranaceus ; Caspase-3 ; Cell cycle ; Cell migration ; Cell viability ; Diabetes ; Diabetes mellitus ; Disease prevention ; Drug dosages ; Endothelial cells ; Flow cytometry ; G1 phase ; Glucose ; Mortality ; Oxidative stress ; Reactive oxygen species ; Review ; Umbilical vein</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-12</ispartof><rights>Copyright © 2020 Yonghui Yin et al.</rights><rights>Copyright © 2020 Yonghui Yin et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Yonghui Yin et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-c0363c4ffb572501d8befe9fc32eb5488d8c609daf7b4ea9b2a7ea2992f518343</citedby><cites>FETCH-LOGICAL-c448t-c0363c4ffb572501d8befe9fc32eb5488d8c609daf7b4ea9b2a7ea2992f518343</cites><orcidid>0000-0003-3655-3566 ; 0000-0002-5590-3632</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714576/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714576/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Makino, Toshiaki</contributor><creatorcontrib>Li, Xiaoli</creatorcontrib><creatorcontrib>Wang, Zichen</creatorcontrib><creatorcontrib>Zhang, Xinying</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Zhu, Ranran</creatorcontrib><creatorcontrib>Chen, Yumin</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Yin, Yonghui</creatorcontrib><creatorcontrib>Zhou, Ji</creatorcontrib><title>Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress</title><title>Evidence-based complementary and alternative medicine</title><description><![CDATA[Vascular endothelial dysfunction is an essential and early sign of diabetic macroangiopathy, a primary complication of diabetes mellitus. Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate the effect of the granule form of the extract produced from the dried root of Astragalus membranaceous (AM) combination with the granule form of the extract produced from the dried Angelica sinensis (AS) on diabetic macroangiopathy and its underlying mechanism. Herein, rats were treated by AM-AS at a ratio of 3 : 2 via intragastric administration. High glucose-induced human umbilical vein vascular endothelial cells (HUVECs) were then treated with drug-containing serum collected from the rats. In high glucose-treated HUVECs, AM-AS combination increased cell viability (P<0.05), decreased the percentage of apoptotic cells (P<0.05) and the expression of the proapoptosis protein caspase 3 (P<0.05), reduced the proportion of cells in the G0/G1 phase (P<0.05), decreased reactive oxygen species level (P<0.05), enhanced cell migration and invasion (P<0.05), and reduced the level of 8-iso-prostaglandin F2alpha. These results indicate that AM-AS combination at the ratio of 3 : 2 ameliorated HUVEC dysfunction by regulating apoptosis, cell migration, and invasion, which might be mediated by their regulatory effect on reactive oxygen species production. The current study provides a theoretical basis for the treatment of diabetic macroangiopathy using AM-AS.]]></description><subject>Angelica sinensis</subject><subject>Apoptosis</subject><subject>Astragalus membranaceus</subject><subject>Caspase-3</subject><subject>Cell cycle</subject><subject>Cell migration</subject><subject>Cell viability</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Disease prevention</subject><subject>Drug dosages</subject><subject>Endothelial cells</subject><subject>Flow cytometry</subject><subject>G1 phase</subject><subject>Glucose</subject><subject>Mortality</subject><subject>Oxidative stress</subject><subject>Reactive oxygen species</subject><subject>Review</subject><subject>Umbilical vein</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtv1DAUhSMEoqWwY40ssUGCoX7FdjZIo6FApUpd8BA769qxZ1wldrGTQv8EvxmHGRXBhpUf59O5Pj5N85Tg14S07SnFFJ8KzIhU9F5zTCQnK06Vun-3l1-PmkelXGFMOynlw-aIMdqxTnXHzc9NGk2IMIUUUfJoXaYMWxjmgkY3mgwRrEv1BLFH67h1Q7CASogullDQeqwXKcPkCvoCxc4DZHQW-zTtqgAD2rhhQG9vi5-j_T3D3KLzuAsmTCFu0eWP0NfZNw59nLIr5XHzwMNQ3JPDetJ8fnf2afNhdXH5_nyzvlhZztW0spgJZrn3ppW0xaRXxnnXecuoMy1XqldW4K4HLw130BkK0gHtOupbohhnJ82bve_1bEbXWxdr7EFf5zBCvtUJgv5biWGnt-lGS0l4K0U1eHEwyOnb7Mqkx1BsDQtx-S5NuVBCMCqWWc__Qa_SnGONt1CyxUKSxfDVnrI5lZKdv3sMwXopWi9F60PRFX-5x3ch9vA9_I9-tqddZZyHPzRpRdXZL0FltGU</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Li, Xiaoli</creator><creator>Wang, Zichen</creator><creator>Zhang, Xinying</creator><creator>Li, Li</creator><creator>Zhu, Ranran</creator><creator>Chen, Yumin</creator><creator>Li, Hui</creator><creator>Yin, Yonghui</creator><creator>Zhou, Ji</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3655-3566</orcidid><orcidid>https://orcid.org/0000-0002-5590-3632</orcidid></search><sort><creationdate>2020</creationdate><title>Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress</title><author>Li, Xiaoli ; Wang, Zichen ; Zhang, Xinying ; Li, Li ; Zhu, Ranran ; Chen, Yumin ; Li, Hui ; Yin, Yonghui ; Zhou, Ji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-c0363c4ffb572501d8befe9fc32eb5488d8c609daf7b4ea9b2a7ea2992f518343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angelica sinensis</topic><topic>Apoptosis</topic><topic>Astragalus membranaceus</topic><topic>Caspase-3</topic><topic>Cell cycle</topic><topic>Cell migration</topic><topic>Cell viability</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Disease prevention</topic><topic>Drug dosages</topic><topic>Endothelial cells</topic><topic>Flow cytometry</topic><topic>G1 phase</topic><topic>Glucose</topic><topic>Mortality</topic><topic>Oxidative stress</topic><topic>Reactive oxygen species</topic><topic>Review</topic><topic>Umbilical vein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaoli</creatorcontrib><creatorcontrib>Wang, Zichen</creatorcontrib><creatorcontrib>Zhang, Xinying</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Zhu, Ranran</creatorcontrib><creatorcontrib>Chen, Yumin</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Yin, Yonghui</creatorcontrib><creatorcontrib>Zhou, Ji</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaoli</au><au>Wang, Zichen</au><au>Zhang, Xinying</au><au>Li, Li</au><au>Zhu, Ranran</au><au>Chen, Yumin</au><au>Li, Hui</au><au>Yin, Yonghui</au><au>Zhou, Ji</au><au>Makino, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract><![CDATA[Vascular endothelial dysfunction is an essential and early sign of diabetic macroangiopathy, a primary complication of diabetes mellitus. Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate the effect of the granule form of the extract produced from the dried root of Astragalus membranaceous (AM) combination with the granule form of the extract produced from the dried Angelica sinensis (AS) on diabetic macroangiopathy and its underlying mechanism. Herein, rats were treated by AM-AS at a ratio of 3 : 2 via intragastric administration. High glucose-induced human umbilical vein vascular endothelial cells (HUVECs) were then treated with drug-containing serum collected from the rats. In high glucose-treated HUVECs, AM-AS combination increased cell viability (P<0.05), decreased the percentage of apoptotic cells (P<0.05) and the expression of the proapoptosis protein caspase 3 (P<0.05), reduced the proportion of cells in the G0/G1 phase (P<0.05), decreased reactive oxygen species level (P<0.05), enhanced cell migration and invasion (P<0.05), and reduced the level of 8-iso-prostaglandin F2alpha. These results indicate that AM-AS combination at the ratio of 3 : 2 ameliorated HUVEC dysfunction by regulating apoptosis, cell migration, and invasion, which might be mediated by their regulatory effect on reactive oxygen species production. The current study provides a theoretical basis for the treatment of diabetic macroangiopathy using AM-AS.]]></abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33293989</pmid><doi>10.1155/2020/6031782</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-3655-3566</orcidid><orcidid>https://orcid.org/0000-0002-5590-3632</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Angelica sinensis Apoptosis Astragalus membranaceus Caspase-3 Cell cycle Cell migration Cell viability Diabetes Diabetes mellitus Disease prevention Drug dosages Endothelial cells Flow cytometry G1 phase Glucose Mortality Oxidative stress Reactive oxygen species Review Umbilical vein |
| title | Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress |
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