Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD
Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis...
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| creator | Tang, Wen-fu Wan, Mei-hua Long, Dan Zhao, Xian-lin Kang, Hong-xin Miao, Yi-fan Zhu, Lv Xiao-yu, Dai Hu, Qian Li, Juan Yao, Jia-qi |
| description | Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis of NAFLD. Sheng-Jiang Powder (SJP), an empirical Chinese medicine formula to treat NAFLD, showed great hepatoprotective properties, but the impact on gut microbiota has never been identified. Therefore, we performed this study to investigate the effect of SJP on gut microbiota in NAFLD mice. Methods. NAFLD was induced by 12 weeks’ high-fat diet (HFD) feeding. Mice were treated with SJP/normal saline daily for 6 weeks. Blood samples were obtained for serum biochemical indices and inflammatory cytokines measurement. Liver tissues were obtained for pathological evaluation and oil red O staining. The expression of lipid metabolism-related genes was quantified by RT-PCR and Western blotting. Changes in gut microbiota composition were analyzed by the 16s rDNA sequencing technique. Results. HFD feeding induced significant increase in bodyweight and serum levels of TG, TC, ALT, and AST. The pathological examination revealed obvious hepatic steatosis in HFD feeding mice. Coadministration of SJP effectively protected against bodyweight increase and lipid accumulation in blood and liver. Increased expression of PPARγ mRNA was observed in HFD feeding mice, but a steady elevation of PPARγ protein level was only found in SJP-treated mice. Meanwhile, the expression of FASN was much higher in HFD feeding mice. Microbiome analysis revealed obvious changes in gut microbiota composition among diverse groups. SJP treatment modulated the relative abundance of short-chain fatty acids (SCFAs) producing bacteria, including norank-f-Erysipelotrichaceae and Roseburia. Conclusions. SJP is efficient in attenuating HFD-induced NAFLD, and it might be partly attributed to the regulation of gut microbiota. |
| doi_str_mv | 10.1155/2020/6697638 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7714571</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2468660584</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-9690ac62014dbbbbe7fd89488d7136792c087f1fcc20f57d98ee6aab5bc86913</originalsourceid><addsrcrecordid>eNqN0ctrVDEUBvAgiq3VnWsJuBH02jzuzUk2hdJ22spUBbtwF3KTk5mUmaTeh8X_3jvMOD5WZpNAfnzk5CPkJWfvOW-aY8EEO1bKgJL6ETnkUPOqFlo_3p_h6wF51vd3jAkDAE_JgZTCSGPEIbm5iBH9QEukX5aYF9WH5PKCfi4PATtaMr0cB3qTfFfaVAZHU6ZXabGsZm6g5wmH6jqH0WOgH09n8_Pn5El0qx5f7PYjcju7uD27quafLq_PTueVr4EPlVGGOa8E43Vop4UQgza11gG4VGCEZxoij94LFhsIRiMq59qm9VoZLo_IyTb2fmzXGDzmoXMre9-ltet-2OKS_fsmp6VdlO8WgNcNbALe7AK68m3EfrDr1HtcrVzGMvZW1EorxRpdT_T1P_SujF2eptsoaJjSUkzq3VZNH9X3Hcb9Yzizm5rspia7q2nir_4cYI9_9TKBt1uwTDm4h_SfcTgZjO635g0wCfInNxCiZQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2467506832</pqid></control><display><type>article</type><title>Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Tang, Wen-fu ; Wan, Mei-hua ; Long, Dan ; Zhao, Xian-lin ; Kang, Hong-xin ; Miao, Yi-fan ; Zhu, Lv ; Xiao-yu, Dai ; Hu, Qian ; Li, Juan ; Yao, Jia-qi</creator><contributor>Zhang, Hongwei ; Hongwei Zhang</contributor><creatorcontrib>Tang, Wen-fu ; Wan, Mei-hua ; Long, Dan ; Zhao, Xian-lin ; Kang, Hong-xin ; Miao, Yi-fan ; Zhu, Lv ; Xiao-yu, Dai ; Hu, Qian ; Li, Juan ; Yao, Jia-qi ; Zhang, Hongwei ; Hongwei Zhang</creatorcontrib><description>Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis of NAFLD. Sheng-Jiang Powder (SJP), an empirical Chinese medicine formula to treat NAFLD, showed great hepatoprotective properties, but the impact on gut microbiota has never been identified. Therefore, we performed this study to investigate the effect of SJP on gut microbiota in NAFLD mice. Methods. NAFLD was induced by 12 weeks’ high-fat diet (HFD) feeding. Mice were treated with SJP/normal saline daily for 6 weeks. Blood samples were obtained for serum biochemical indices and inflammatory cytokines measurement. Liver tissues were obtained for pathological evaluation and oil red O staining. The expression of lipid metabolism-related genes was quantified by RT-PCR and Western blotting. Changes in gut microbiota composition were analyzed by the 16s rDNA sequencing technique. Results. HFD feeding induced significant increase in bodyweight and serum levels of TG, TC, ALT, and AST. The pathological examination revealed obvious hepatic steatosis in HFD feeding mice. Coadministration of SJP effectively protected against bodyweight increase and lipid accumulation in blood and liver. Increased expression of PPARγ mRNA was observed in HFD feeding mice, but a steady elevation of PPARγ protein level was only found in SJP-treated mice. Meanwhile, the expression of FASN was much higher in HFD feeding mice. Microbiome analysis revealed obvious changes in gut microbiota composition among diverse groups. SJP treatment modulated the relative abundance of short-chain fatty acids (SCFAs) producing bacteria, including norank-f-Erysipelotrichaceae and Roseburia. Conclusions. SJP is efficient in attenuating HFD-induced NAFLD, and it might be partly attributed to the regulation of gut microbiota.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/6697638</identifier><identifier>PMID: 33293992</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Chinese medicine ; Cirrhosis ; Cytokines ; Diet ; Drug dosages ; Fatty acids ; Fatty liver ; Feeding ; Feeds ; Gene expression ; Hepatocellular carcinoma ; High fat diet ; Inflammation ; Insulin resistance ; Intestinal microflora ; Lipid metabolism ; Lipids ; Liver cirrhosis ; Liver diseases ; Liver transplantation ; Medical research ; Metabolism ; Microbiomes ; Microbiota ; mRNA ; Oxidative stress ; Pathogenesis ; Polymerase chain reaction ; Public health ; rRNA 16S ; Serum levels ; Steatosis ; Transplants & implants ; Western blotting</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-15</ispartof><rights>Copyright © 2020 Juan Li et al.</rights><rights>Copyright © 2020 Juan Li et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Juan Li et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-9690ac62014dbbbbe7fd89488d7136792c087f1fcc20f57d98ee6aab5bc86913</citedby><cites>FETCH-LOGICAL-c471t-9690ac62014dbbbbe7fd89488d7136792c087f1fcc20f57d98ee6aab5bc86913</cites><orcidid>0000-0001-8137-5610 ; 0000-0002-1237-9455 ; 0000-0002-7822-910X ; 0000-0002-4088-7975 ; 0000-0001-8212-0134 ; 0000-0002-4302-3339 ; 0000-0002-5775-9355 ; 0000-0002-2110-2941 ; 0000-0002-5783-7085 ; 0000-0002-3483-2345 ; 0000-0001-9294-6634</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714571/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714571/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4014,27914,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33293992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Hongwei</contributor><contributor>Hongwei Zhang</contributor><creatorcontrib>Tang, Wen-fu</creatorcontrib><creatorcontrib>Wan, Mei-hua</creatorcontrib><creatorcontrib>Long, Dan</creatorcontrib><creatorcontrib>Zhao, Xian-lin</creatorcontrib><creatorcontrib>Kang, Hong-xin</creatorcontrib><creatorcontrib>Miao, Yi-fan</creatorcontrib><creatorcontrib>Zhu, Lv</creatorcontrib><creatorcontrib>Xiao-yu, Dai</creatorcontrib><creatorcontrib>Hu, Qian</creatorcontrib><creatorcontrib>Li, Juan</creatorcontrib><creatorcontrib>Yao, Jia-qi</creatorcontrib><title>Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis of NAFLD. Sheng-Jiang Powder (SJP), an empirical Chinese medicine formula to treat NAFLD, showed great hepatoprotective properties, but the impact on gut microbiota has never been identified. Therefore, we performed this study to investigate the effect of SJP on gut microbiota in NAFLD mice. Methods. NAFLD was induced by 12 weeks’ high-fat diet (HFD) feeding. Mice were treated with SJP/normal saline daily for 6 weeks. Blood samples were obtained for serum biochemical indices and inflammatory cytokines measurement. Liver tissues were obtained for pathological evaluation and oil red O staining. The expression of lipid metabolism-related genes was quantified by RT-PCR and Western blotting. Changes in gut microbiota composition were analyzed by the 16s rDNA sequencing technique. Results. HFD feeding induced significant increase in bodyweight and serum levels of TG, TC, ALT, and AST. The pathological examination revealed obvious hepatic steatosis in HFD feeding mice. Coadministration of SJP effectively protected against bodyweight increase and lipid accumulation in blood and liver. Increased expression of PPARγ mRNA was observed in HFD feeding mice, but a steady elevation of PPARγ protein level was only found in SJP-treated mice. Meanwhile, the expression of FASN was much higher in HFD feeding mice. Microbiome analysis revealed obvious changes in gut microbiota composition among diverse groups. SJP treatment modulated the relative abundance of short-chain fatty acids (SCFAs) producing bacteria, including norank-f-Erysipelotrichaceae and Roseburia. Conclusions. SJP is efficient in attenuating HFD-induced NAFLD, and it might be partly attributed to the regulation of gut microbiota.</description><subject>Chinese medicine</subject><subject>Cirrhosis</subject><subject>Cytokines</subject><subject>Diet</subject><subject>Drug dosages</subject><subject>Fatty acids</subject><subject>Fatty liver</subject><subject>Feeding</subject><subject>Feeds</subject><subject>Gene expression</subject><subject>Hepatocellular carcinoma</subject><subject>High fat diet</subject><subject>Inflammation</subject><subject>Insulin resistance</subject><subject>Intestinal microflora</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liver cirrhosis</subject><subject>Liver diseases</subject><subject>Liver transplantation</subject><subject>Medical research</subject><subject>Metabolism</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>mRNA</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>rRNA 16S</subject><subject>Serum levels</subject><subject>Steatosis</subject><subject>Transplants & implants</subject><subject>Western 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of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD</title><author>Tang, Wen-fu ; Wan, Mei-hua ; Long, Dan ; Zhao, Xian-lin ; Kang, Hong-xin ; Miao, Yi-fan ; Zhu, Lv ; Xiao-yu, Dai ; Hu, Qian ; Li, Juan ; Yao, Jia-qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-9690ac62014dbbbbe7fd89488d7136792c087f1fcc20f57d98ee6aab5bc86913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chinese medicine</topic><topic>Cirrhosis</topic><topic>Cytokines</topic><topic>Diet</topic><topic>Drug dosages</topic><topic>Fatty acids</topic><topic>Fatty liver</topic><topic>Feeding</topic><topic>Feeds</topic><topic>Gene expression</topic><topic>Hepatocellular carcinoma</topic><topic>High fat diet</topic><topic>Inflammation</topic><topic>Insulin resistance</topic><topic>Intestinal microflora</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Liver cirrhosis</topic><topic>Liver diseases</topic><topic>Liver transplantation</topic><topic>Medical research</topic><topic>Metabolism</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>mRNA</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>rRNA 16S</topic><topic>Serum levels</topic><topic>Steatosis</topic><topic>Transplants & implants</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Wen-fu</creatorcontrib><creatorcontrib>Wan, Mei-hua</creatorcontrib><creatorcontrib>Long, Dan</creatorcontrib><creatorcontrib>Zhao, Xian-lin</creatorcontrib><creatorcontrib>Kang, Hong-xin</creatorcontrib><creatorcontrib>Miao, Yi-fan</creatorcontrib><creatorcontrib>Zhu, Lv</creatorcontrib><creatorcontrib>Xiao-yu, Dai</creatorcontrib><creatorcontrib>Hu, Qian</creatorcontrib><creatorcontrib>Li, 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Hong-xin</au><au>Miao, Yi-fan</au><au>Zhu, Lv</au><au>Xiao-yu, Dai</au><au>Hu, Qian</au><au>Li, Juan</au><au>Yao, Jia-qi</au><au>Zhang, Hongwei</au><au>Hongwei Zhang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>15</epage><pages>1-15</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis of NAFLD. Sheng-Jiang Powder (SJP), an empirical Chinese medicine formula to treat NAFLD, showed great hepatoprotective properties, but the impact on gut microbiota has never been identified. Therefore, we performed this study to investigate the effect of SJP on gut microbiota in NAFLD mice. Methods. NAFLD was induced by 12 weeks’ high-fat diet (HFD) feeding. Mice were treated with SJP/normal saline daily for 6 weeks. Blood samples were obtained for serum biochemical indices and inflammatory cytokines measurement. Liver tissues were obtained for pathological evaluation and oil red O staining. The expression of lipid metabolism-related genes was quantified by RT-PCR and Western blotting. Changes in gut microbiota composition were analyzed by the 16s rDNA sequencing technique. Results. HFD feeding induced significant increase in bodyweight and serum levels of TG, TC, ALT, and AST. The pathological examination revealed obvious hepatic steatosis in HFD feeding mice. Coadministration of SJP effectively protected against bodyweight increase and lipid accumulation in blood and liver. Increased expression of PPARγ mRNA was observed in HFD feeding mice, but a steady elevation of PPARγ protein level was only found in SJP-treated mice. Meanwhile, the expression of FASN was much higher in HFD feeding mice. Microbiome analysis revealed obvious changes in gut microbiota composition among diverse groups. SJP treatment modulated the relative abundance of short-chain fatty acids (SCFAs) producing bacteria, including norank-f-Erysipelotrichaceae and Roseburia. Conclusions. SJP is efficient in attenuating HFD-induced NAFLD, and it might be partly attributed to the regulation of gut microbiota.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33293992</pmid><doi>10.1155/2020/6697638</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-8137-5610</orcidid><orcidid>https://orcid.org/0000-0002-1237-9455</orcidid><orcidid>https://orcid.org/0000-0002-7822-910X</orcidid><orcidid>https://orcid.org/0000-0002-4088-7975</orcidid><orcidid>https://orcid.org/0000-0001-8212-0134</orcidid><orcidid>https://orcid.org/0000-0002-4302-3339</orcidid><orcidid>https://orcid.org/0000-0002-5775-9355</orcidid><orcidid>https://orcid.org/0000-0002-2110-2941</orcidid><orcidid>https://orcid.org/0000-0002-5783-7085</orcidid><orcidid>https://orcid.org/0000-0002-3483-2345</orcidid><orcidid>https://orcid.org/0000-0001-9294-6634</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Chinese medicine Cirrhosis Cytokines Diet Drug dosages Fatty acids Fatty liver Feeding Feeds Gene expression Hepatocellular carcinoma High fat diet Inflammation Insulin resistance Intestinal microflora Lipid metabolism Lipids Liver cirrhosis Liver diseases Liver transplantation Medical research Metabolism Microbiomes Microbiota mRNA Oxidative stress Pathogenesis Polymerase chain reaction Public health rRNA 16S Serum levels Steatosis Transplants & implants Western blotting |
| title | Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T05%3A29%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Sheng-Jiang%20Powder%20on%20Gut%20Microbiota%20in%20High-Fat%20Diet-Induced%20NAFLD&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Tang,%20Wen-fu&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=15&rft.pages=1-15&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2020/6697638&rft_dat=%3Cproquest_pubme%3E2468660584%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2467506832&rft_id=info:pmid/33293992&rfr_iscdi=true |