Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir
To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) Wes...
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| Veröffentlicht in: | Ecology and evolution 2020-12, Vol.10 (23), p.13403-13411 |
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| creator | Johnson, Benziwa Nathalie Quashie, Marie Luce Akossiwoa Chaix, Gilles Camus‐Kulandaivelu, Letizia Adjonou, Kossi Segla, Kossi Novinyo Kokutse, Adzo Dzifa Ouinsavi, Christine Bationo, Babou André Rabiou, Habou Kokou, Kouami Vignes, Hélène |
| description | To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
In order to understand the genetic structure of populations and to assess the genetic diversity of Pterocarpus erinaceus (Fabaceae) in West Africa, genetic markers, in this case nuclear microsatellites, were isolated from individuals come from 4 countries, Togo, Benin, Burkina Faso, and Niger. The knowledge acquired will allow us to understand the functioning and evolution of the species in order to implement effective conservation strategies essential for the sustainable management of genetic resources. |
| doi_str_mv | 10.1002/ece3.6944 |
| format | Article |
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In order to understand the genetic structure of populations and to assess the genetic diversity of Pterocarpus erinaceus (Fabaceae) in West Africa, genetic markers, in this case nuclear microsatellites, were isolated from individuals come from 4 countries, Togo, Benin, Burkina Faso, and Niger. The knowledge acquired will allow us to understand the functioning and evolution of the species in order to implement effective conservation strategies essential for the sustainable management of genetic resources.</description><identifier>ISSN: 2045-7758</identifier><identifier>EISSN: 2045-7758</identifier><identifier>DOI: 10.1002/ece3.6944</identifier><identifier>PMID: 33304547</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>African tree species ; Deoxyribonucleic acid ; DNA ; Ecology, environment ; Endemic species ; Genetic diversity ; Genetic markers ; Heterozygosity ; International trade ; Life Sciences ; Markers ; Microsatellites ; next‐generation sequencing (NGS) ; nuclear microsatellites ; Original Research ; Polymorphism ; Population differentiation ; Population genetics ; Pterocarpus erinaceus ; Species diversity ; Threatened species ; Trees ; Wildlife conservation ; Wood</subject><ispartof>Ecology and evolution, 2020-12, Vol.10 (23), p.13403-13411</ispartof><rights>2020 The Authors. published by John Wiley & Sons Ltd</rights><rights>2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4774-7b6bb70920cf90694952be1d66b590aaf234f84c1a7fb7dcbecf6afbd4fcfa4f3</citedby><cites>FETCH-LOGICAL-c4774-7b6bb70920cf90694952be1d66b590aaf234f84c1a7fb7dcbecf6afbd4fcfa4f3</cites><orcidid>0000-0003-0018-8085 ; 0000-0003-2015-0551</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713909/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713909/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33304547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03014633$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, Benziwa Nathalie</creatorcontrib><creatorcontrib>Quashie, Marie Luce Akossiwoa</creatorcontrib><creatorcontrib>Chaix, Gilles</creatorcontrib><creatorcontrib>Camus‐Kulandaivelu, Letizia</creatorcontrib><creatorcontrib>Adjonou, Kossi</creatorcontrib><creatorcontrib>Segla, Kossi Novinyo</creatorcontrib><creatorcontrib>Kokutse, Adzo Dzifa</creatorcontrib><creatorcontrib>Ouinsavi, Christine</creatorcontrib><creatorcontrib>Bationo, Babou André</creatorcontrib><creatorcontrib>Rabiou, Habou</creatorcontrib><creatorcontrib>Kokou, Kouami</creatorcontrib><creatorcontrib>Vignes, Hélène</creatorcontrib><title>Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir</title><title>Ecology and evolution</title><addtitle>Ecol Evol</addtitle><description>To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
In order to understand the genetic structure of populations and to assess the genetic diversity of Pterocarpus erinaceus (Fabaceae) in West Africa, genetic markers, in this case nuclear microsatellites, were isolated from individuals come from 4 countries, Togo, Benin, Burkina Faso, and Niger. The knowledge acquired will allow us to understand the functioning and evolution of the species in order to implement effective conservation strategies essential for the sustainable management of genetic resources.</description><subject>African tree species</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecology, environment</subject><subject>Endemic species</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Heterozygosity</subject><subject>International trade</subject><subject>Life Sciences</subject><subject>Markers</subject><subject>Microsatellites</subject><subject>next‐generation sequencing (NGS)</subject><subject>nuclear microsatellites</subject><subject>Original Research</subject><subject>Polymorphism</subject><subject>Population differentiation</subject><subject>Population genetics</subject><subject>Pterocarpus erinaceus</subject><subject>Species diversity</subject><subject>Threatened 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Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7X2</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0018-8085</orcidid><orcidid>https://orcid.org/0000-0003-2015-0551</orcidid></search><sort><creationdate>202012</creationdate><title>Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir</title><author>Johnson, Benziwa Nathalie ; Quashie, Marie Luce Akossiwoa ; Chaix, Gilles ; Camus‐Kulandaivelu, Letizia ; Adjonou, Kossi ; Segla, Kossi Novinyo ; Kokutse, Adzo Dzifa ; Ouinsavi, Christine ; Bationo, Babou André ; Rabiou, Habou ; Kokou, Kouami ; Vignes, Hélène</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4774-7b6bb70920cf90694952be1d66b590aaf234f84c1a7fb7dcbecf6afbd4fcfa4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>African tree species</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ecology, environment</topic><topic>Endemic species</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Heterozygosity</topic><topic>International trade</topic><topic>Life Sciences</topic><topic>Markers</topic><topic>Microsatellites</topic><topic>next‐generation sequencing (NGS)</topic><topic>nuclear microsatellites</topic><topic>Original Research</topic><topic>Polymorphism</topic><topic>Population differentiation</topic><topic>Population genetics</topic><topic>Pterocarpus erinaceus</topic><topic>Species diversity</topic><topic>Threatened species</topic><topic>Trees</topic><topic>Wildlife conservation</topic><topic>Wood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, Benziwa Nathalie</creatorcontrib><creatorcontrib>Quashie, Marie Luce Akossiwoa</creatorcontrib><creatorcontrib>Chaix, Gilles</creatorcontrib><creatorcontrib>Camus‐Kulandaivelu, Letizia</creatorcontrib><creatorcontrib>Adjonou, Kossi</creatorcontrib><creatorcontrib>Segla, Kossi Novinyo</creatorcontrib><creatorcontrib>Kokutse, Adzo Dzifa</creatorcontrib><creatorcontrib>Ouinsavi, Christine</creatorcontrib><creatorcontrib>Bationo, Babou 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UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Ecology and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnson, Benziwa Nathalie</au><au>Quashie, Marie Luce Akossiwoa</au><au>Chaix, Gilles</au><au>Camus‐Kulandaivelu, Letizia</au><au>Adjonou, Kossi</au><au>Segla, Kossi Novinyo</au><au>Kokutse, Adzo Dzifa</au><au>Ouinsavi, Christine</au><au>Bationo, Babou André</au><au>Rabiou, Habou</au><au>Kokou, Kouami</au><au>Vignes, Hélène</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir</atitle><jtitle>Ecology and evolution</jtitle><addtitle>Ecol Evol</addtitle><date>2020-12</date><risdate>2020</risdate><volume>10</volume><issue>23</issue><spage>13403</spage><epage>13411</epage><pages>13403-13411</pages><issn>2045-7758</issn><eissn>2045-7758</eissn><abstract>To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
In order to understand the genetic structure of populations and to assess the genetic diversity of Pterocarpus erinaceus (Fabaceae) in West Africa, genetic markers, in this case nuclear microsatellites, were isolated from individuals come from 4 countries, Togo, Benin, Burkina Faso, and Niger. The knowledge acquired will allow us to understand the functioning and evolution of the species in order to implement effective conservation strategies essential for the sustainable management of genetic resources.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>33304547</pmid><doi>10.1002/ece3.6944</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-0018-8085</orcidid><orcidid>https://orcid.org/0000-0003-2015-0551</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | African tree species Deoxyribonucleic acid DNA Ecology, environment Endemic species Genetic diversity Genetic markers Heterozygosity International trade Life Sciences Markers Microsatellites next‐generation sequencing (NGS) nuclear microsatellites Original Research Polymorphism Population differentiation Population genetics Pterocarpus erinaceus Species diversity Threatened species Trees Wildlife conservation Wood |
| title | Isolation and characterization of microsatellite markers for the threatened African endemic tree species Pterocarpus erinaceus Poir |
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