Hyperpolarized NMR Metabolomics at Natural 13C Abundance
Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on 1H 1D spectroscopy, severely limited by peak overlap. 13C NMR benefits from a larger signal dispersion but...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-11, Vol.92 (22), p.14867-14871 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Dey, Arnab Charrier, Benoît Martineau, Estelle Deborde, Catherine Gandriau, Elodie Moing, Annick Jacob, Daniel Eshchenko, Dmitry Schnell, Marc Melzi, Roberto Kurzbach, Dennis Ceillier, Morgan Chappuis, Quentin Cousin, Samuel F Kempf, James G Jannin, Sami Dumez, Jean-Nicolas Giraudeau, Patrick |
| description | Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on 1H 1D spectroscopy, severely limited by peak overlap. 13C NMR benefits from a larger signal dispersion but is barely used in metabolomics due to ca. 6000-fold lower sensitivity. We introduce a new approach, based on hyperpolarized 13C NMR at natural abundance, that circumvents this limitation. A new untargeted NMR-based metabolomic workflow based on dissolution dynamic nuclear polarization (d-DNP) for the first time enabled hyperpolarized natural abundance 13C metabolomics. Statistical analysis of resulting hyperpolarized 13C data distinguishes two groups of plant (tomato) extracts and highlights biomarkers, in full agreement with previous results on the same biological model. We also optimize parameters of the semiautomated d-DNP system suitable for high-throughput studies. |
| doi_str_mv | 10.1021/acs.analchem.0c03510 |
| format | Article |
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Most NMR metabolomic studies are based on 1H 1D spectroscopy, severely limited by peak overlap. 13C NMR benefits from a larger signal dispersion but is barely used in metabolomics due to ca. 6000-fold lower sensitivity. We introduce a new approach, based on hyperpolarized 13C NMR at natural abundance, that circumvents this limitation. A new untargeted NMR-based metabolomic workflow based on dissolution dynamic nuclear polarization (d-DNP) for the first time enabled hyperpolarized natural abundance 13C metabolomics. Statistical analysis of resulting hyperpolarized 13C data distinguishes two groups of plant (tomato) extracts and highlights biomarkers, in full agreement with previous results on the same biological model. 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| source | American Chemical Society Journals |
| subjects | Abundance Analytical chemistry Biological models (mathematics) Biomarkers Chemistry Letter Metabolomics NMR Nuclear magnetic resonance Plant extracts Spectroscopy Statistical analysis Tomatoes Workflow |
| title | Hyperpolarized NMR Metabolomics at Natural 13C Abundance |
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