Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape ( Brassica napus L.) against Fungal Pathogens
Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of BRZ63, a new endophyte of oilseed rape ( L.) against W70, K, K2291, and . In addition, features crucial for biocontrol, plant growth promotion, and colonization were...
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| Veröffentlicht in: | International journal of molecular sciences 2020-11, Vol.21 (22), p.8740 |
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| creator | Chlebek, Daria Pinski, Artur Żur, Joanna Michalska, Justyna Hupert-Kocurek, Katarzyna |
| description | Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of
BRZ63, a new endophyte of oilseed rape (
L.) against
W70,
K,
K2291, and
. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of
Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion. |
| doi_str_mv | 10.3390/ijms21228740 |
| format | Article |
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BRZ63, a new endophyte of oilseed rape (
L.) against
W70,
K,
K2291, and
. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of
Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21228740</identifier><identifier>PMID: 33228091</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acetic acid ; Ammonia ; Ammonia - metabolism ; Ammonia - pharmacology ; Arabidopsis - microbiology ; Ascomycota - drug effects ; Ascomycota - growth & development ; Ascomycota - pathogenicity ; Bacteria ; Bacterial Proteins - classification ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Biological control ; Biological Control Agents - chemistry ; Biological Control Agents - metabolism ; Biosurfactants ; Brassica napus ; Brassica napus - microbiology ; Carbon-Carbon Lyases - biosynthesis ; Carbon-Carbon Lyases - pharmacology ; Colletotrichum - drug effects ; Colletotrichum - growth & development ; Colletotrichum - pathogenicity ; Colonization ; Data Mining - methods ; Endophytes ; Endophytes - genetics ; Endophytes - metabolism ; Enzymes ; Exopolysaccharides ; Fungi ; Fusarium - drug effects ; Fusarium - growth & development ; Fusarium - pathogenicity ; Genome, Bacterial ; Genomes ; Germination ; Hydrocarbons ; In vitro methods and tests ; Indoleacetic acid ; Indoleacetic Acids - metabolism ; Indoleacetic Acids - pharmacology ; Metabolites ; Microorganisms ; Motility ; Oilseeds ; Pathogens ; Phylogeny ; Plant diseases ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant Roots - microbiology ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - pharmacology ; Pseudomonas fluorescens ; Pseudomonas fluorescens - classification ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - metabolism ; Rape plants ; Rapeseed ; Rhizoctonia - drug effects ; Rhizoctonia - growth & development ; Rhizoctonia - pathogenicity ; Secondary metabolites ; Seedlings ; Seedlings - microbiology ; Seeds ; Siderophores ; Siderophores - biosynthesis ; Siderophores - pharmacology ; Solubilization ; Surface-Active Agents - metabolism ; Surface-Active Agents - pharmacology ; Surfactants</subject><ispartof>International journal of molecular sciences, 2020-11, Vol.21 (22), p.8740</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-22face0674e39357d2c652139b54d02d2282eac0c111ac1915e6034c9033cf9a3</citedby><cites>FETCH-LOGICAL-c412t-22face0674e39357d2c652139b54d02d2282eac0c111ac1915e6034c9033cf9a3</cites><orcidid>0000-0002-2496-6200 ; 0000-0003-2821-568X ; 0000-0002-2616-9539 ; 0000-0001-7467-7418</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699435/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699435/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33228091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chlebek, Daria</creatorcontrib><creatorcontrib>Pinski, Artur</creatorcontrib><creatorcontrib>Żur, Joanna</creatorcontrib><creatorcontrib>Michalska, Justyna</creatorcontrib><creatorcontrib>Hupert-Kocurek, Katarzyna</creatorcontrib><title>Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape ( Brassica napus L.) against Fungal Pathogens</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of
BRZ63, a new endophyte of oilseed rape (
L.) against
W70,
K,
K2291, and
. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of
Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.</description><subject>Acetic acid</subject><subject>Ammonia</subject><subject>Ammonia - metabolism</subject><subject>Ammonia - pharmacology</subject><subject>Arabidopsis - microbiology</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - growth & development</subject><subject>Ascomycota - pathogenicity</subject><subject>Bacteria</subject><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biological control</subject><subject>Biological Control Agents - chemistry</subject><subject>Biological Control Agents - metabolism</subject><subject>Biosurfactants</subject><subject>Brassica napus</subject><subject>Brassica napus - microbiology</subject><subject>Carbon-Carbon Lyases - biosynthesis</subject><subject>Carbon-Carbon Lyases - pharmacology</subject><subject>Colletotrichum - drug effects</subject><subject>Colletotrichum - growth & development</subject><subject>Colletotrichum - pathogenicity</subject><subject>Colonization</subject><subject>Data Mining - methods</subject><subject>Endophytes</subject><subject>Endophytes - genetics</subject><subject>Endophytes - metabolism</subject><subject>Enzymes</subject><subject>Exopolysaccharides</subject><subject>Fungi</subject><subject>Fusarium - drug effects</subject><subject>Fusarium - growth & development</subject><subject>Fusarium - pathogenicity</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Germination</subject><subject>Hydrocarbons</subject><subject>In vitro methods and tests</subject><subject>Indoleacetic acid</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>Metabolites</subject><subject>Microorganisms</subject><subject>Motility</subject><subject>Oilseeds</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant Roots - microbiology</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - pharmacology</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - classification</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - metabolism</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Rhizoctonia - drug effects</subject><subject>Rhizoctonia - growth & development</subject><subject>Rhizoctonia - pathogenicity</subject><subject>Secondary metabolites</subject><subject>Seedlings</subject><subject>Seedlings - microbiology</subject><subject>Seeds</subject><subject>Siderophores</subject><subject>Siderophores - biosynthesis</subject><subject>Siderophores - pharmacology</subject><subject>Solubilization</subject><subject>Surface-Active Agents - metabolism</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Surfactants</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkk1P3DAQhq2qVaFLbz1XlnqhEgv-yEd9QeqihVbawgrRSy_R4EyyXiV2ajsg_hE_s46gaNuTR5pnXs87M4R84OxYSsVOzLYPggvxpczYK7LPMyHmjBXl6514j7wLYcuYkCJXb8melIlniu-Txwu0rkf6w1hjWwq2pss76EaIxlnqGho3SBfGaWejdx1du4g2Guim3DrgWLveWQi06UbnMWi0gS6ufxXyiAK9xHu6tLUbNg8Rp4or0wXEml7DgPSQLjyEYDRQC8MY6Or4M4UWjA2Rno-2Tb-sIW5cm0QPyJsGUvH753dGfp4vb86-zVdXF9_Pvq7mOuMizoVoQGOynKFUMi9roYtccKlu86xmok6-BYJmmnMOmiueY8FkphWTUjcK5IycPukO422PdfITPXTV4E0P_qFyYKp_M9ZsqtbdVWWhVCbzJHD4LODd7xFDrHqTxtJ1YNGNoRJZkfGcTcuYkU__oVs3epvsTZQslcpTWzNy9ERp70Lw2Lw0w1k1nUC1ewIJ_7hr4AX-u3P5B7CQrXY</recordid><startdate>20201119</startdate><enddate>20201119</enddate><creator>Chlebek, Daria</creator><creator>Pinski, Artur</creator><creator>Żur, Joanna</creator><creator>Michalska, Justyna</creator><creator>Hupert-Kocurek, Katarzyna</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2496-6200</orcidid><orcidid>https://orcid.org/0000-0003-2821-568X</orcidid><orcidid>https://orcid.org/0000-0002-2616-9539</orcidid><orcidid>https://orcid.org/0000-0001-7467-7418</orcidid></search><sort><creationdate>20201119</creationdate><title>Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape ( Brassica napus L.) against Fungal Pathogens</title><author>Chlebek, Daria ; Pinski, Artur ; Żur, Joanna ; Michalska, Justyna ; Hupert-Kocurek, Katarzyna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-22face0674e39357d2c652139b54d02d2282eac0c111ac1915e6034c9033cf9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetic acid</topic><topic>Ammonia</topic><topic>Ammonia - metabolism</topic><topic>Ammonia - pharmacology</topic><topic>Arabidopsis - microbiology</topic><topic>Ascomycota - drug effects</topic><topic>Ascomycota - growth & development</topic><topic>Ascomycota - pathogenicity</topic><topic>Bacteria</topic><topic>Bacterial Proteins - classification</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Biological control</topic><topic>Biological Control Agents - chemistry</topic><topic>Biological Control Agents - metabolism</topic><topic>Biosurfactants</topic><topic>Brassica napus</topic><topic>Brassica napus - microbiology</topic><topic>Carbon-Carbon Lyases - biosynthesis</topic><topic>Carbon-Carbon Lyases - pharmacology</topic><topic>Colletotrichum - drug effects</topic><topic>Colletotrichum - growth & development</topic><topic>Colletotrichum - pathogenicity</topic><topic>Colonization</topic><topic>Data Mining - methods</topic><topic>Endophytes</topic><topic>Endophytes - genetics</topic><topic>Endophytes - metabolism</topic><topic>Enzymes</topic><topic>Exopolysaccharides</topic><topic>Fungi</topic><topic>Fusarium - drug effects</topic><topic>Fusarium - growth & development</topic><topic>Fusarium - pathogenicity</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Germination</topic><topic>Hydrocarbons</topic><topic>In vitro methods and tests</topic><topic>Indoleacetic acid</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>Metabolites</topic><topic>Microorganisms</topic><topic>Motility</topic><topic>Oilseeds</topic><topic>Pathogens</topic><topic>Phylogeny</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Plant Roots - microbiology</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - pharmacology</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - classification</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - metabolism</topic><topic>Rape plants</topic><topic>Rapeseed</topic><topic>Rhizoctonia - drug effects</topic><topic>Rhizoctonia - growth & development</topic><topic>Rhizoctonia - pathogenicity</topic><topic>Secondary metabolites</topic><topic>Seedlings</topic><topic>Seedlings - microbiology</topic><topic>Seeds</topic><topic>Siderophores</topic><topic>Siderophores - biosynthesis</topic><topic>Siderophores - pharmacology</topic><topic>Solubilization</topic><topic>Surface-Active Agents - metabolism</topic><topic>Surface-Active Agents - pharmacology</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chlebek, Daria</creatorcontrib><creatorcontrib>Pinski, Artur</creatorcontrib><creatorcontrib>Żur, Joanna</creatorcontrib><creatorcontrib>Michalska, Justyna</creatorcontrib><creatorcontrib>Hupert-Kocurek, Katarzyna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chlebek, Daria</au><au>Pinski, Artur</au><au>Żur, Joanna</au><au>Michalska, Justyna</au><au>Hupert-Kocurek, Katarzyna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape ( Brassica napus L.) against Fungal Pathogens</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-11-19</date><risdate>2020</risdate><volume>21</volume><issue>22</issue><spage>8740</spage><pages>8740-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of
BRZ63, a new endophyte of oilseed rape (
L.) against
W70,
K,
K2291, and
. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of
Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33228091</pmid><doi>10.3390/ijms21228740</doi><orcidid>https://orcid.org/0000-0002-2496-6200</orcidid><orcidid>https://orcid.org/0000-0003-2821-568X</orcidid><orcidid>https://orcid.org/0000-0002-2616-9539</orcidid><orcidid>https://orcid.org/0000-0001-7467-7418</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2020-11, Vol.21 (22), p.8740 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7699435 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acetic acid Ammonia Ammonia - metabolism Ammonia - pharmacology Arabidopsis - microbiology Ascomycota - drug effects Ascomycota - growth & development Ascomycota - pathogenicity Bacteria Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Biological control Biological Control Agents - chemistry Biological Control Agents - metabolism Biosurfactants Brassica napus Brassica napus - microbiology Carbon-Carbon Lyases - biosynthesis Carbon-Carbon Lyases - pharmacology Colletotrichum - drug effects Colletotrichum - growth & development Colletotrichum - pathogenicity Colonization Data Mining - methods Endophytes Endophytes - genetics Endophytes - metabolism Enzymes Exopolysaccharides Fungi Fusarium - drug effects Fusarium - growth & development Fusarium - pathogenicity Genome, Bacterial Genomes Germination Hydrocarbons In vitro methods and tests Indoleacetic acid Indoleacetic Acids - metabolism Indoleacetic Acids - pharmacology Metabolites Microorganisms Motility Oilseeds Pathogens Phylogeny Plant diseases Plant Diseases - microbiology Plant Diseases - prevention & control Plant Roots - microbiology Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - pharmacology Pseudomonas fluorescens Pseudomonas fluorescens - classification Pseudomonas fluorescens - genetics Pseudomonas fluorescens - metabolism Rape plants Rapeseed Rhizoctonia - drug effects Rhizoctonia - growth & development Rhizoctonia - pathogenicity Secondary metabolites Seedlings Seedlings - microbiology Seeds Siderophores Siderophores - biosynthesis Siderophores - pharmacology Solubilization Surface-Active Agents - metabolism Surface-Active Agents - pharmacology Surfactants |
| title | Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape ( Brassica napus L.) against Fungal Pathogens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T05%3A22%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome%20Mining%20and%20Evaluation%20of%20the%20Biocontrol%20Potential%20of%20Pseudomonas%20fluorescens%20BRZ63,%20a%20New%20Endophyte%20of%20Oilseed%20Rape%20(%20Brassica%20napus%20L.)%20against%20Fungal%20Pathogens&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Chlebek,%20Daria&rft.date=2020-11-19&rft.volume=21&rft.issue=22&rft.spage=8740&rft.pages=8740-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms21228740&rft_dat=%3Cproquest_pubme%3E2463799503%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2463799503&rft_id=info:pmid/33228091&rfr_iscdi=true |