Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots
The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators of plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusiv...
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| Veröffentlicht in: | International journal of molecular sciences 2020-11, Vol.21 (22), p.8441 |
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| creator | Aslam, Mohammad Sugita, Kenji Qin, Yuan Rahman, Abidur |
| description | The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators of plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA (miR) in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of
with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4 °C revealed that the auxin signaling mutant, solitary root 1 (
; mutation in
), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRs in the wild-type and
mutant roots using next-generation sequencing revealed 180 known and 71 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26-31 nt small RNA population in
compared with wild type. Comparative analysis of microRNA expression shows significant differential expression of 13 known and 7 novel miRs in
at 4 °C compared with wild type. Target gene expression analysis of the members from one potential candidate miR, miR169, revealed the possible involvement of miR169/
module in the Aux/IAA14-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRs in auxin and cold responses. |
| doi_str_mv | 10.3390/ijms21228441 |
| format | Article |
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with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4 °C revealed that the auxin signaling mutant, solitary root 1 (
; mutation in
), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRs in the wild-type and
mutant roots using next-generation sequencing revealed 180 known and 71 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26-31 nt small RNA population in
compared with wild type. Comparative analysis of microRNA expression shows significant differential expression of 13 known and 7 novel miRs in
at 4 °C compared with wild type. Target gene expression analysis of the members from one potential candidate miR, miR169, revealed the possible involvement of miR169/
module in the Aux/IAA14-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRs in auxin and cold responses.</description><identifier>ISSN: 1422-0067</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21228441</identifier><identifier>PMID: 33182739</identifier><language>eng</language><publisher>Switzerland: MDPI</publisher><subject>Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - physiology ; CCAAT-Binding Factor - genetics ; CCAAT-Binding Factor - metabolism ; Cold-Shock Response - genetics ; Cold-Shock Response - physiology ; Gene Expression Regulation, Plant ; Genes, Plant ; High-Throughput Nucleotide Sequencing ; Indoleacetic Acids - metabolism ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Mutation ; Plant Roots - genetics ; Plant Roots - physiology ; RNA, Plant - genetics ; RNA, Plant - metabolism ; Sequence Analysis, RNA ; Signal Transduction ; Transcription Factors - genetics ; Transcription Factors - physiology</subject><ispartof>International journal of molecular sciences, 2020-11, Vol.21 (22), p.8441</ispartof><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-83c7156a1b714c01e6bfa4e1d771f5e62908ace245d168067e72822bcecd22a03</citedby><cites>FETCH-LOGICAL-c493t-83c7156a1b714c01e6bfa4e1d771f5e62908ace245d168067e72822bcecd22a03</cites><orcidid>0000-0003-4713-6151 ; 0000-0002-3910-1699 ; 0000-0003-3006-899X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697755/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697755/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33182739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aslam, Mohammad</creatorcontrib><creatorcontrib>Sugita, Kenji</creatorcontrib><creatorcontrib>Qin, Yuan</creatorcontrib><creatorcontrib>Rahman, Abidur</creatorcontrib><title>Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators of plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA (miR) in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of
with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4 °C revealed that the auxin signaling mutant, solitary root 1 (
; mutation in
), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRs in the wild-type and
mutant roots using next-generation sequencing revealed 180 known and 71 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26-31 nt small RNA population in
compared with wild type. Comparative analysis of microRNA expression shows significant differential expression of 13 known and 7 novel miRs in
at 4 °C compared with wild type. Target gene expression analysis of the members from one potential candidate miR, miR169, revealed the possible involvement of miR169/
module in the Aux/IAA14-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRs in auxin and cold responses.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - physiology</subject><subject>CCAAT-Binding Factor - genetics</subject><subject>CCAAT-Binding Factor - metabolism</subject><subject>Cold-Shock Response - genetics</subject><subject>Cold-Shock Response - physiology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Indoleacetic Acids - metabolism</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Mutation</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - physiology</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>Sequence Analysis, RNA</subject><subject>Signal Transduction</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><issn>1422-0067</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1Lw0AQXUSxWr15lhw9GLtfySYXIRQ_CtVC1fOy2Z3ULUk2ZhPRf2-ktdTTDDOPN2_eQ-iC4BvGUjyx68pTQmnCOTlAJ4RTGmIci8O9foROvV9jTBmN0mM0YowkVLD0BC2y_msyyzLCgyWs-lJ14IPK6tYtn7PwCYwdJiaYutIEL10L3g8437jaQ2DrIGtVbo1rvB3mznX-DB0VqvRwvq1j9HZ_9zp9DOeLh9k0m4eap6wLE6YFiWJFckG4xgTivFAciBGCFBHENMWJ0kB5ZEicDB-AoAmluQZtKFWYjdHthrfp8wqMhrprVSmb1laq_ZZOWfl_U9t3uXKfUsSpEFE0EFxtCVr30YPvZGW9hrJUNbjeS8pjLOKIDHLH6HoDHVzxvoVid4Zg-ZuB3M9ggF_uS9uB_0xnPxBjglc</recordid><startdate>20201110</startdate><enddate>20201110</enddate><creator>Aslam, Mohammad</creator><creator>Sugita, Kenji</creator><creator>Qin, Yuan</creator><creator>Rahman, Abidur</creator><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4713-6151</orcidid><orcidid>https://orcid.org/0000-0002-3910-1699</orcidid><orcidid>https://orcid.org/0000-0003-3006-899X</orcidid></search><sort><creationdate>20201110</creationdate><title>Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots</title><author>Aslam, Mohammad ; Sugita, Kenji ; Qin, Yuan ; Rahman, Abidur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-83c7156a1b714c01e6bfa4e1d771f5e62908ace245d168067e72822bcecd22a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - physiology</topic><topic>CCAAT-Binding Factor - genetics</topic><topic>CCAAT-Binding Factor - metabolism</topic><topic>Cold-Shock Response - genetics</topic><topic>Cold-Shock Response - physiology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Indoleacetic Acids - metabolism</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Mutation</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - physiology</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>Sequence Analysis, RNA</topic><topic>Signal Transduction</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aslam, Mohammad</creatorcontrib><creatorcontrib>Sugita, Kenji</creatorcontrib><creatorcontrib>Qin, Yuan</creatorcontrib><creatorcontrib>Rahman, Abidur</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aslam, Mohammad</au><au>Sugita, Kenji</au><au>Qin, Yuan</au><au>Rahman, Abidur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-11-10</date><risdate>2020</risdate><volume>21</volume><issue>22</issue><spage>8441</spage><pages>8441-</pages><issn>1422-0067</issn><eissn>1422-0067</eissn><abstract>The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators of plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA (miR) in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of
with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4 °C revealed that the auxin signaling mutant, solitary root 1 (
; mutation in
), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRs in the wild-type and
mutant roots using next-generation sequencing revealed 180 known and 71 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26-31 nt small RNA population in
compared with wild type. Comparative analysis of microRNA expression shows significant differential expression of 13 known and 7 novel miRs in
at 4 °C compared with wild type. Target gene expression analysis of the members from one potential candidate miR, miR169, revealed the possible involvement of miR169/
module in the Aux/IAA14-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRs in auxin and cold responses.</abstract><cop>Switzerland</cop><pub>MDPI</pub><pmid>33182739</pmid><doi>10.3390/ijms21228441</doi><orcidid>https://orcid.org/0000-0003-4713-6151</orcidid><orcidid>https://orcid.org/0000-0002-3910-1699</orcidid><orcidid>https://orcid.org/0000-0003-3006-899X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - physiology CCAAT-Binding Factor - genetics CCAAT-Binding Factor - metabolism Cold-Shock Response - genetics Cold-Shock Response - physiology Gene Expression Regulation, Plant Genes, Plant High-Throughput Nucleotide Sequencing Indoleacetic Acids - metabolism MicroRNAs - genetics MicroRNAs - metabolism Mutation Plant Roots - genetics Plant Roots - physiology RNA, Plant - genetics RNA, Plant - metabolism Sequence Analysis, RNA Signal Transduction Transcription Factors - genetics Transcription Factors - physiology |
| title | Aux/IAA14 Regulates microRNA-Mediated Cold Stress Response in Arabidopsis Roots |
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