NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach

Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled with NMR‐active nuclei. We report on the incorporation of 13...

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Veröffentlicht in:	Angewandte Chemie International Edition 2020-11, Vol.59 (46), p.20659-20665
Hauptverfasser:	Jonker, Hendrik R. A., Saxena, Krishna, Shcherbakova, Aleksandra, Tiemann, Birgit, Bakker, Hans, Schwalbe, Harald
Format:	Artikel
Sprache:	eng
Schlagworte:	C-mannosylation conformation analysis Glycan Glycoproteins Mannose NMR NMR spectroscopy Nuclear magnetic resonance Polysaccharides Protein folding Protein structure Proteins selective isotopic labeling Thrombospondin Tryptophan
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